composition fractions and structure of leaf protein from green biomass

Isolation and characterization of anticoagulant protein from the venom of hemachatus haemachatus (african ringhals cobra

Ngày tải lên : 14/09/2015, 13:36

... proteins Aim and scope of the thesis 60 Chapter Two Purification of the Anticoagulant Protein Isolation and Purification of hemextin A and hemextin B Assessment of homogeneity of hemextin A and ... activation of protein C Activated protein C counteracts coagulation by cleaving and inhibiting the cofactors FVa and FVIIIa The free form of protein S in blood serves as cofactor to activated protein ... Rechromatography of hemextin A and B ESI-MS of hemextin A and B Comparison of amino acid sequence of hemextin A and hemextin B with other sequences of the three-finger toxin family CD spectra Effects of hemextins...

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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

Ngày tải lên : 07/03/2014, 03:20

... constituent of the Tic motor complex (see below) The present minireview provides a short description of recent advances in the understanding of the channel-, motor- and regulatory components of the ... the Toc and Tic complexes Tic22 may be involved in the stabilization of the Toc/Tic/preprotein supercomplex In this model, Tic110 forms the channel protein and also acts in the recruitment of Hsp93 ... motif of the mammalian co-chaperones Hsp70-interacting protein (Hip) and Hsp70/Hsp90organizing protein (Hop) and (b) the region immediately preceding this domain is predicted to form a structure...

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Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldﬁsh pot

Ngày tải lên : 07/03/2014, 10:20

... domains of goldfish RICH (gRICH68), human and rat CNPase (hCNP and rCNP, respectively) and a homologous protein from puffer fish (Gi:47207595) The secondary structure elements refer to the solution structure ... similarity of these proteins suggests a similar function The structure of RICH and nucleotide binding studies presented here represent another step towards understanding of CNP ⁄ RICH function and suggest ... representation of the RICH catalytic domain Secondary structure elements and the N- and C-termini are labeled (C) Backbone overlay of catalytic domains of goldfish RICH (in cyan) and human CNPase...

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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

Ngày tải lên : 07/03/2014, 16:20

... 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... h of IPTG induction (Fig 1) Puriﬁcation and refolding of Rv1399c Given the large quantity of insoluble material obtained [up to 160 mg of protein from L of cell culture (Table 1, Fig lane 4)], ... consists of three domains The largest domain encompasses strands b1 to b6 and strand b7 to the C-terminus The central b sheet is composed of parallel b strands associated to an antiparallel strand...

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Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

Ngày tải lên : 08/03/2014, 02:20

... factor of 18.8% and an Rfree of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2) The resulting model is depicted in Fig The Ca backbones of the ... 1) All a-helices and b-strands are labeled Fig B-factor plots of the main chains of the two molecules of CDase in the asymmetric unit The averages of molecules A (solid line) and B (broken ˚ ˚ ... chains of Asp125, Asp146, Asn119 and Asn124, the main-chain oxygens of Gly144 and Asp121, and by a water molecule Ca-II stabilizes a surface region far away from the active center The B factors of...

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Báo cáo khoa học: Phosphorylation mechanism and structure of serine-arginine protein kinases pot

Ngày tải lên : 15/03/2014, 00:20

... SRPK1 and SRSF1, together with crystal structures of SRPK1 bound to peptide and protein substrates and the recent structures of CLK1 and CLK3, suggest an elegant mechanism of recognition and phosphorylation ... SRPK structure and mechanism G Ghosh and J A Adams spliceosome, a macromolecular complex composed of ﬁve small nuclear ribonucleoproteins (U1, U2, U4, U5, and U6) and over 100 auxiliary proteins ... entry of SR proteins It has been shown that phosphorylation of the RS domain leads to enhanced interactions with the nuclear import receptor, transportin SR, and entry of SRPK structure and mechanism...

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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

Ngày tải lên : 17/03/2014, 03:20

... crystal structure of Anx(Gh1) from cotton emphasizes the high conservation of the unique annexin fold even among the members of the plant subfamily of annexin proteins The fold is comprised of the ... shows separation of the two modules (I/IV and II/III) leading to greater accessibility of the intermodular space than in the case of AnxA5 and to formation of a Table Conservation of salt bridges ... binding loops The IAB loops of Anx(Gh1) (A) and AnxA5 (B) are shown in the same view from the front (C) and (D) show the IIAB loops of Anx(Gh1) and AnxA5, respectively, from the top (membrane-binding)...

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Báo cáo khoa học: A peptide derived from cyclin-dependent kinase activator (p35) speciﬁcally inhibits Cdk5 activity and phosphorylation of tau protein in transfected cells pdf

Ngày tải lên : 23/03/2014, 21:21

... were fixed and double-stained with polyclonal antip35 (C-19) and anti-AT8 Ig (a, c, and e) and with polyclonal antip35 (N-20) and anti-AT8 Ig (b, d, and f) a and b expressed transfected p25 and p35, ... large number of proteins including NF-H and NF-M, neurofilament high and middle molecular-mass proteins [31], and the tau protein [25] Cdk5 hyperactivity is toxic to cultured neurons and may underlie ... Repositioning of the T-loop in both cases appears to be a consequence of the rigidity of the cyclin box structure of the activator compared to the relatively more flexible T-loop Judging from the crystal structure, ...

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Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc

Ngày tải lên : 31/03/2014, 01:20

... unpublished observations) Under conditions of dilute protein and in the absence of free PLP, loss of oligomer structure is accompanied by immediate release of cofactor from the StP subunit Therefore, it ... mL of a solution of 0.4 M NaCl, 0.7% (w/v) SDS and mgÆmL)1 proteinase K (Sigma) in 10 mM Tris/HCl buffer, pH 8.2 After incubation at 50 °C for h, protein was precipitated with mL of M NaCl and ... Identiﬁcation of dimer contact regions in StP from the structural alignment of StP with other GPs An alignment of the amino acid sequences of StP, rmGP and maltodextrin phosphorylase from Escherichia...

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Management ownership structure, audit quality and impairment of assets--evidence from China

Ngày tải lên : 01/06/2014, 14:02

... words, the demand for financial reporting by the public and the government's participation in setting and enforcing rules and standards affect the ownership structure of enterprises and in turn ... academic professionals, Professor Ferdinand Gul, Professor Jeong Bong Kim, Professor Bin Srinidi, Professor T.J Wong, Dr Shimin Chen, Dr Peter Cheng, Dr Agnes Lo, Dr Raymond Wong, Dr Xiaodong Xu and ... decision of writing down the value of assets and the magnitude of write-down allow management of listed companies to exercise discretion in determining the recoverable value of relevant assets and...

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Báo cáo khoa học: "Structure and development of vegetative buds, from the lower crown of Picea abies" pptx

Ngày tải lên : 08/08/2014, 19:21

... iii) dates of onset and termination of shoot elongation; iv) seasonal changes of starch accumulation in the embryonic shoot; and v) changes in the metabolism of tannin vacuoles weekly, and 07.11, ... termination of shoot growth, as well as the rate of shoot elongation, were established on branches from the same part of the crown of trees Terminal and distal lateral buds/shoots were measured weekly from ... There crown 1992 - from 03.05 to 11.10 of vegetative buds from the in Picea abies was studied weekly Also information was used from another study on the same clone and on ramets of the same age...

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the meaning and structure of a narrative a systemic functional analysis

Ngày tải lên : 07/09/2013, 13:48

... relationships of co-ordination (or parataxis the linking of elements of equal status, the initiating and continuing elements are free) and subordination (or hypotaxis the linking of elements of unequal ... model of language which comprises levels of language phonology, lexico-grammar, and semantics and the social of language in which language occurs Furthermore, different from the other models of ... rationale of the study, the aims of the study, scope of the study, methods of the study, data collection, and the research design 3 Chapter two: The literature review provides some fundamental and...

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Tài liệu Báo cáo " The meaning and structure of a science fiction story: a sysyemic functional analysis " doc

Ngày tải lên : 12/02/2014, 20:20

... meaning and structure of a science fiction story: A sysyemic 39 said to the man We can breathe the air its a mixture of oxygen and R:P R:P R:D nitrogen. Both of them took off their helmets and ... mixture of oxygen and nitrogen. (16) ||| Both of them took off the helmets (17) || and breathed deeply.||| (18) || They looked at everything carefully (19) || All the plants and animals looked new and ... astronauts landing in a new planet for the first time The what is going on? of the text is well represented in the experiential component of meaning From the point of view of transitivity, of the...

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**Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc**

Ngày tải lên : 16/02/2014, 15:20

... Cu(I)4,Cu(I)4MT3 and Cu(I)6,Cu(I)6MT3] The determination of a 3D structure seems crucial for understanding the differences between Cu–MT3 and Cu–MT1 ⁄ (structure also not known) and the presence and role of ... Thiolate is a soft ligand and therefore shows a preference for soft metals Moreover the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ion ... in the protein The modiﬁcation of the surface-accessible sulfur of cysteine ligands is thought to be the key that unlocks the metals from the protein [38] Thus, in general, reactions of the cysteine...

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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

Ngày tải lên : 18/02/2014, 11:20

... PDI family proteins of yeast and animals [9–12], but this is the first report to confirm expression of such proteins in the ER of plants A CXXS motif and a CXXC motif in the N-terminal and C-terminal ... folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer Biochemistry 30, 613–619 43 Morjana NA & Gilbert HF (1991) Effect of protein and ... S1 Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S2 Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants...

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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx

Ngày tải lên : 19/02/2014, 07:20

... leads to formation of the active complex, whereas binding of a minimum of m Mn2+ ions forms the inactive complex The sizes of the active and inactive fractions are the result of competitive metal ... binding to RNA The values of n and m are estimated from Hill analysis of G-dependent cleavage of 23S.5DGb It should be noted that Scheme indicates only the initial and nal states of the preRNA; it does ... the P9 subdomains, most of P9 is protected, but most of P9.1 is neutral The 5Â strand of P9.0 is protected despite the absence of the 3Â strand For the idiosyncratic P7.1 and P7.2 domains, the...

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Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf

Ngày tải lên : 20/02/2014, 04:20

... Usage of all this knowledge allows us to select the correct value of 1.695m and reject other values Thus we can increase the precision of value extraction by ﬁnding and analyzing an entire group of ... the acquisition of numerical attributes from the Web and attempt to deal with ambiguity and noise of the retrieved attribute values (Aramaki et al., 2007) utilize a small set of patterns to extract ... the textual context of these values A signiﬁcant body of recent research deals with extraction of various data from web tables and lists (e.g., (Cafarella et al., 2008; Crestan and Pantel, 2010))...

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Tài liệu Báo cáo khoa học: Structure of peptidase T from Salmonella typhimurium doc

Ngày tải lên : 21/02/2014, 03:20

... sites of APP, CG2 and peptidase T have homologous zinc ion ligands and are more closely related to each other than to LAP and CPA The similarity between the structure of the second domain in CG2 and ... observations) The amino-acid sequences of LAP and CPA are not similar and the three-dimensional structures of these proteins are too different from that of peptidase T to allow a meaningful superimposition ... for APP The position of this ligand and comparison of the zinc ions and the binding of amastatin to LAP suggest the location of the S1 subsite As peptidase T cleaves a variety of tripeptides, albeit...

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Tài liệu logic and structure of the computer game potx

Ngày tải lên : 23/02/2014, 19:20

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Báo cáo khoa học: Branched chain mechanism of polymerization and ultrastructure of prion protein amyloid ﬁbrils ppt

Ngày tải lên : 07/03/2014, 10:20

... conformational stability of the scrapie prion protein Protein Sci 10, 854–863 Kuczius T & Groschup MH (1999) Differences in proteinase K resistance and neuronal deposition of abnormal prion proteins characterize ... cross-b-sheet structure of amyloid fibrils is substantially more stable kinetically and thermodynamically than the structure of nonfibrillar oligomeric species, ensuring that fibrils remain assembled and ... for extraction and purification of PrPSc involved sonication, treatment with detergents and, sometimes, freezing and thawing [49–51] All of these steps cause severe fragmentation of fibrils In our...

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