complete the sentence with the correct form of the adjective julián y francisco son
Tài liệu Supply the correct form of the verbs1 docx
Ngày tải lên :
23/01/2014, 07:20
... snake.
14. My brother managed to kill the snake just at the time when I were almost exhausted.
Supply the correct form of the verbs
1. Cats could fly if they (have) wings.
2. If Peter (study) harder, ... Cats could fly if they (have) had wings.
2. If Peter (study) studied harder, he would get better marks.
3. You will be late for class if you (not, hurry) don't hurry
4. Mary would not have ... better marks.
3. You will be late for class if you (not, hurry).
4. Mary would not have got wet if she (wear) a raincoat.
5. If today (be) a holiday, I would stay in bed for all day long.
6. If...
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Báo cáo khoa học: The pharmacological chaperone isofagomine increases the activity of the Gaucher disease L444P mutant form of b-glucosidase docx
Ngày tải lên :
22/03/2014, 21:20
... c-octyldeoxynojorimycin
[33]. As a result of the very low level of GCase activity
in the L444P fibroblasts, alternative methods were
developed to reduce the amount of IFG in the assay,
thereby increasing ... 38% of non-Jewish
patients with Gaucher disease carry the L444P allele
[5–7].
Currently, enzyme replacement therapy (ERT) and
small-molecule substrate reduction therapy (SRT) are
the only approved ... can
increase wild-type human GCase activity in vitro [25].
The ability of IFG to cross the blood–brain barrier
and to increase the activity of L444P GCase encour-
Effect of isofagomine on L444P...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
Ngày tải lên :
07/03/2014, 21:20
... subfamily
of GPCRs.
The aim of this study was to understand the structural
requirements at this key locus of the V
1a
R which endow
high affinity agonist binding, by systematically mutating the
critical ... rescued
by a chimeric construct in which the N-terminus of the
OTR is replaced with the corresponding sequence of
the V
1a
R [10]. The role of the N-terminus has been
addressed recently by alanine-scanning ... receptor protein. In contrast to the three classes of
antagonist, the binding of AVP was dramatically affected
by the substitution of Arg46, with the affinity of AVP for all
the 19 mutant receptor constructs...
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Economic Impact of the Abolition of the Milk Quota Regime – Regional Analysis of the Milk Production in the EU – doc
Ngày tải lên :
18/03/2014, 00:20
...
assessment of possible implications of the dairy policy reform, with an explicit focus on
regional effects in the EU-27 of a milk quota abolition in the year 2015.
Specification of the Model
The ... just 7% of global dairy
Overview of the EU dairy sector
- 7 -
Besides the differences in the number of dairy cows between the MS, the number of dairy
cows also varies significantly at regional ... on average, with +35.5% in the EU-10 and
+13.8 in the EU-15. This excess supply makes the net trade position of the EU-27 stronger
towards the Rest of the World. Within the EU there is a quite...
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Báo cáo khoa học: Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization pptx
Ngày tải lên :
23/03/2014, 07:20
... together,
these data suggest that the RS-rich region of the SARS-
CoV N protein is possibly phosphorylated by SRPK1.
Phosphorylation of multiple serines within the
RS motif
The RS motif of the ... activity of the SARS-CoV N protein
is modulated by phosphorylation. Translation of an in vitro tran-
scribed fire y luciferase mRNA was performed in reticulocyte
lysate in the presence of different ... crosslinking of the N
protein confirmed that phosphorylated N was less
capable of forming oligomers than the nonphosphory-
lated one (Fig. 4E). Therefore, it is likely that phos-
phorylation of the RS...
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Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
Ngày tải lên :
23/03/2014, 17:22
... 2D), locates
the residue side chain hydroxyl proton.
Comparison to the alternate crystal structures
The pattern of NOESY cross peaks of the Tyr66(E7) ring
to the heme (Fig. 4A–C), Tyr32(B10) (Fig. ... supported by the relaxation
properties of the C
e
Hs signal (see above). The hydroxyl
proton position is not directly determined in the crystal
structure, but can be inferred by the position of other ... in the immediate vicinity. The
proposal that the Tyr32(B10) hydroxyl proton acted as a
donor to the carbonyl of Tyr66(E7) in the rWT
metHbH
2
O crystal structure [16] places it % 5.9 A
˚
from
theironwithanangleof%...
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Báo cáo khoa học: Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164 doc
Ngày tải lên :
23/03/2014, 18:20
... all the other monosaccharides
were confirmed by GC-MS data of the methylated products
to be as presented in Scheme 1.
Deamination of the products of complete deacylation of
the LPS led to the ... analysis of com-
pound 4 was the determination of the anomeric configur-
ation of Kdo D (see above).
Mild acid hydrolysis of the LPS with subsequent borohy-
dride reduction and separation of the ... was depolymerized by acid methano-
lysis, treated with NaBD
4
to reduce carboxy groups,
hydrolysed, reduced with NaBD
4
, and acetylated. The
second procedure led to the reduction of the GlcA...
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Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
Ngày tải lên :
23/03/2014, 21:20
... enzymes typically have high
levels of cytochrome c reductase activity, with electron
transfer to the cytochrome mediated by the FMN cofactor.
A more substantial increase in specific activity for ... recon-
stituted only % 2% of the activity of the full length CPR. In
thecaseofNR1,thek
cat
for the full length enzyme is low
(1.3 s
)1
) and is clearly gated largely by the hydride transfer
event [1]. While ... previously [1]. For
purification of the FMN domain, cell suspensions were
lysed by incubating at 30 °C for 15 min in the presence of
100 lgặmL
)1
lysozyme, followed by 30 min at 4 Cinthe
presence of...
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Báo cáo khoa học: Structure of the O-polysaccharide fromProteus myxofaciens Classification of the bacterium into a newProteusO-serogroup pptx
Ngày tải lên :
23/03/2014, 21:20
... of
glucuronic acid (GlcA). The
D
configuration of GlcN and
GalN was established by GLC of the acetylated (S)-2-butyl
glycosides. The
D
configuration of GlcA was determined by
analysis of the
13
C NMR chemical-shift ... spectrum of the O-polysac-
charide of P. myxofaciens.
3184 Z. Sidorczyk et al.(Eur. J. Biochem. 270) Ó FEBS 2003
Structure of the O-polysaccharide from
Proteus myxofaciens
Classification of the bacterium ... Shashkov &
Knirel, Y. A. (1998) Identification of N
e
-[(R)-1-carboxyethyl]-
L
-lysine in, and the complete structure of, the repeating unit of the
O-specific polysaccharide of Providencia alcalifaciens...
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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot
Ngày tải lên :
30/03/2014, 10:20
... extracellular
domain of the human alpha7 acetylcholine receptor
expressed in yeast: replacement of the hydrophobic
cysteine loop by the hydrophilic loop of the ACh-bind-
ing protein enhances protein solubility. ... to verify the identity of the inserts.
Yeast transformation and dot-blot screening
of positive clones
Plasmids (10 lg) encoding the b1–221 ECD (with or with-
out the FLAG epitope) and the various ... mass
of 29 kDa (Fig. 2C), confirming that the proteins
were glycosylated.
Gel-filtration analysis of polypeptides
To examine the solubility and oligomerization state of
the recombinant polypeptides,...
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Báo cáo khoa học: Role of the N- and C-terminal regions of the PufX protein in the structural organization of the photosynthetic core complex of Rhodobacter sphaeroides pptx
Ngày tải lên :
31/03/2014, 09:20
... ability f or photosynthetic growth, the
insertion of PufX in the core LH1–RC complex, the stability
of the dimers and the kinetics of flash-induced reduction of
cytochrome b
561
of the cytochrome ... LH1-a polypeptide has an inhibitory effect
on the formation of the LH1 complex. This result suggests
that the central core of the PufX protein is responsible of the
break in the continuity of the ... monitoring the reduction of the
cytochrome b
561
induced by a short actinic light fl ash in the
presence of the inhibitor a ntimycin A [ 29]. Reduction of
the cytochrome b
561
typically shows a...
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Review of the Literature Regarding Critical Information Needs of the American Public
Ngày tải lên :
02/06/2014, 09:39
... the
fulfillment of these information needs.
Clearly then, policy-relevant research must capture the increasingly complex functioning
of local media systems in ways that fully account for the ... systems within the analysis of the ways different kinds of local
communities actually function, and the extent to which local community information needs
are being effectively served.
5) The ... account of the increasing failure of the news ecosystem to adequately
report on education nationally and locally, with some suggestions for the formation of a non-
profit reporting sector. The third,...
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Báo cáo sinh học: " Viroporin potential of the lentivirus lytic peptide (LLP) domains of the HIV-1 gp41 protein" docx
Ngày tải lên :
18/06/2014, 18:20
... of the
repeated charged residues found on the hydrophilic sur-
face of LLP-1 and -2. This discovery has led to the theory
that the LLP-3 domain of TM may play a role in oligomer-
ization of the ... synthesized by solid-phase synthesis meth-
odology using a semi-automated peptide synthesizer and
conventional N-alpha-9-fluorenylmethyloxycarbonyl
(Fmoc) chemistry. LLP-3 peptides were synthesized ... secondary structures of the LLP domains. (A) Wimley-White hydrophobicity plot of TM
predicted amino acid sequence of HIV
HXB2
. Highlighted cylinders show the locations of the LLP domains. A hydrophibicity...
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