collection processing and banking of umbilical cord blood stem cells for transplantation and regenerative medicine
báo cáo hóa học:" Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels" docx
... impact of the fetal milieu The UC is an exceptional source of vascular cells, which can offer valuable information about the cellular characteristics of the blood vessels of the individual and their ... human umbilical cords (UCs) Cell proliferation kinetics of vascular cell types obtained from human umbilical cords (UCs) Human umbilical artery and vein endothelial (HUAECs and HUVECs, panels A and ... http://www.translational -medicine. com/content/7/1/30 Figure Projection area of human umbilical artery and vein endothelial cells grown to confluence Projection area of human umbilical artery and vein endothelial cells...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates " pps
... mechanism of action of MSCs, and KGF and angiopoietin-1 are Figure Bacterial Growth in vivo (A and B) and in vitro (C) Bacterial colony forming units in BAL fluid (A) and blood (B) at 1, 3, and days ... therapeutic modality for the treatment of ALI Among the various sources of stem cells, human umbilical cord blood (UCB) provides readily available MSCs with low immunogenicity [11-13] Therefore, human ... the number of colony forming units (CFUs) was counted in BAL fluids and blood from animals in the E, F, and M groups at postinjury days 1, 3, and Bacterial counts in both BAL and blood were significantly...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
... ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu erew ,gk 95.0 ± 77.3 dehgiew ... wef a ,revewoh ;noitamrof ytivac fo egatnecrep tsellams eht detartsnomed puorg PC eht ,noitidda nI spuorg GBCU dna CSM-BCU eht ni noitamrof ytivac fo egatnecrep rellams a dnuof ew ,sllec mets detnalpsnart ... sllec detnalpsnart eht rof troppus reffo yam droc derujni eht ,droc lanips eht fo sdne lamixorp dna latsid eht ot noitcennoc rof ,slairetam ro sllec suoirav fo pu edam dloffacs ro egdirb a htiw...
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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
... mesenchymal stem cells from human bone marrow and umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages and ... osteoblast progenitors from umbilical cord blood stem cells by BIO-PLEX technology Cell Biol Int 2008, 32, 320-325 27 Rogers I, Casper RF Umbilical cord blood stem cells Best 393 Pract Res Clin ... precultured with cells in osteogenic medium or vacuumed for uniform loading and retention of cells [13,34] In the present study, β-TCP granules were mixed with cells immediately before implantation;...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury Spinal Cord 2007, 45, 15-24 12 Hipp J, Atala A Sources of stem cells for regenerative ... maintaining cell culture for experimental analysis A guarantee of enough numbers of multipotent stem cells out of a very small quantity of cord blood sample from the canine umbilical cord blood is attractive ... isolation of mesenchymal stem cells from umbilical cord blood Stem Cells 2004, 22, 625-634 Breems DA, Löwenberg B Acute myeloid leukemia and the position of autologous stem cell transplantation Semin...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... concentration and annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and ... from seven days for P1 and P2, 10 days for P3 to three weeks for P4 to P6 The total number of passages before cell growth diminished varied according to the age of the donor sand rat In general, ... multipotent mesenchymal stem cell markers CD105 and CD29 and negative localisation of CD45 and CD34 [23] For cluster of differentiation (CD) immunohistochemical assessment of stem cell markers, AD-MSC...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of protein by lipolytic ... potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma ... isolation and systematic identification of human adipose-derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript to BioMed Central and take full advantage of: ...
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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
... performed with RT step (1 cycle at 50°C for 20 min) followed by initial activation of HotStar Taq DNA Polymerase at 94°C for 15 and 40 cycles in three steps: 94°C for 10 s, 60°C for 30 s, 72°C for ... performed with RT step (1 cycle at 50°C for 20 min) followed by initial activation of HotStar Taq DNA Polymerase at 95°C for 15 and 40 cycles in three steps: 94°C for 10 s, 60°C for 15 s, 72°C for ... of stem cells, such as c-kit, BCRP-1, Notch-1, Sox-2 and BMI-1 (data not shown) To determine whether these cells also expressed the mRNAs of classical HIV receptor CD4 and co-receptor CXCR4 and...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... percentages of MSC surface markers of CD45+ and CD271+ cells did not differ between day and day 14 of cell culture However, compared with day 0, the percentage of cells positive for MSC surface ... for each animal was then determined by summation of all numbers divided by Immunofluorescent (IF) Studies and Crowded Score of Lung Parenchyma IF staining was performed for the examinations of ... (Affidavit of Approval of Animal Use Protocol No 2008121108) and performed in accordance with the Guide for the Care and Use of Laboratory Animals (NIH publication No Page of 13 85-23, National...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
... marrow-derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone marrow-derived MSCs administration on the blood reperfusion ... (No 20100471352 for DZ) and the Social Development Project of Wujin Bureau of Technology(No WS2009009 for YL) Author details Department of Cardiology, the First Affiliated Hospital of Nanjing Medical ... of Cardiology, the Affiliated Wujin Hospital of Jiangsu University, 213002, P.R China 3The Research Center for Bone And Stem Cells, Nanjing Medical University, 210029, P.R China 4Department of...
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Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
... cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal and ... During trauma and disease conditions, loss of tissue may occur and instead of being in homeostasis state, the stem cells migrate out of the niche and start their proliferative and differentiation ... am one of the few who have worked under the very best, both Prof Chan and Prof Seeram To Joan, thank you for being the sweet person that you are I am blessed to know both of you and Prof Chan...
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Báo cáo sinh học: " Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia'''' docx
... Jiang S, Guo SQ: Clinical Efficacy and Safety of Transplantation of Umbilical Cord Blood Source Neural Stem Cells for Multiple Sclerosis Practical Preventive Medicine 2008, 15(4):1186-1188 28 ... PR, Chung YG, Cho TH: Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury Stem Cell Rev 2011, 7(1):181-194 34 Dasari VR, Spomar DG, Gondi CS, Sloffer CA, Saving KL, ... Endothelial Colony Forming Cells and Mesenchymal Stem Cells are Enriched at Different Gestational Ages in Human Umbilical Cord Blood Pediatr Res 2008, 64:68-73 Yelnik A, Bonan I: Clinical tools for assessing...
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báo cáo hóa học:" Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia" pdf
... Jiang S, Guo SQ: Clinical Efficacy and Safety of Transplantation of Umbilical Cord Blood Source Neural Stem Cells for Multiple Sclerosis Practical Preventive Medicine 2008, 15(4):1186-1188 28 ... PR, Chung YG, Cho TH: Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury Stem Cell Rev 2011, 7(1):181-194 34 Dasari VR, Spomar DG, Gondi CS, Sloffer CA, Saving KL, ... Endothelial Colony Forming Cells and Mesenchymal Stem Cells are Enriched at Different Gestational Ages in Human Umbilical Cord Blood Pediatr Res 2008, 64:68-73 Yelnik A, Bonan I: Clinical tools for assessing...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
... Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells ... cardiac myocyte and cardiomyocytes sheets from differentiated human umbilical cord mesenchymal stem cells We assessed viability of the cells of sheets at room temperature No indication of cell apoptosis ... immunostaining of MHC of cells from these groups after and 10 days’ culturing Cells from CMCM and CMCM+S1P groups show strong expression of both a-actinin and MHC proteins, but not those cells from...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow Stem Cells 2007, 25:1384-1392 Weiss ML, Anderson ... for the treatment of inflammatory arthritis [11] Mesenchymal stem cells (MSCs) are cells of stromal origin that can exert profound immunosuppression by modulating T and B cell proliferation and ... Expansion of UC-MSCs in vitro The UC-MSCs were successfully isolated and expanded from all the umbilical cords They had a fibroblast-like morphology, uniformly negative for CD14, CD45, CD34 and HLA-DR,...
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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model
... application for CLECs has been tested in the treatment of a type diabetes mouse model xiv LIST OF FIGURES AND TABLES Figure 3.1 Schematic of human umbilical cord and the isolation of cord lining cells ... characterization of a novel primary epithelial cell-type from the human umbilical cord Two types of cells can be derived from the umbilical cord lining membrane, termed cord lining epithelial cells (CLECs) and ... characterization of definitive MSC markers 2.4 Fetal/neonatal stem cells 2.4.1 Background Fetal and neonatal stem cells come in between embryonic and adult stem cells in the developmental continuum Studies of...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... calculated for the N-terminal antibodies SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-terminal antibodies 6H4, SAF60 and SAF70 for ... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... means and SE according to the antibody used for PrP detection Calculation of the banding patterns of 16 gels using antibody SAF34 gave an SE of 1.2 for the di-glycosylated isoform, 0.9 for the...
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Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf
... hamster cells decreases formation of 8-oxoG in mitochondrial DNA [20], but the role of this protein in protecting nuclear DNA from oxidation in human cells and the mechanisms of regulation of the ... test for the left pair of bars (no treatment) showed P ¼ 0.0032, and for the right pair of bars (menadione) the P ¼ 0.032 In (B) KD-1 cells were treated with 50 lM menadione (MEN) for h, and immunoblotting ... higher abundance of a-satellite and satellite III RNA in KD-1 cells than in control cells (Fig 6C) Therefore, knockdown of the PRDX5 gene stimulated transcription of some families of human satellite...
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