... depth-averaged
model in a nonorthogonal curvilinear coordinates for prediction of flow pattern in
meandering channel with 60
o
and 90
o
bend, and also with compound meandering channel.
In this study, ... surface profile into vertical intake. In this regard, the model showed its
applicability in computing the flow into intake with air-entrainment.
The model was also applied to investigate the ... classified into two groups namely:
the depth-averaged models in Cartesian coordinate system and the depth-averaged models
in generalized curvilinear coordinate system. The basic assumption in deriving...
...
“field, tenor and mode”. Field, tenor and mode determine the experiential meaning,
interpersonal meaning and textual meaning, respectively.
In order to understand a text, apart from its register, ... defining (restrictive) and non-defining (non-restrictive) relative
clauses. According to Halliday (1994: 227) in terms of meaning, these clauses do not
define subsets, in the way that a defining ... main
character’s point of view. He is an old man left in a clearing by his tribe and his son
with only a fire and some wood to burn in a few hours. And during this short moment
he realized and...
... In Chang
cells, stained occludin was observed in the cytoplasmic
region. Additionally, in Occ
WT
-overexpressing Hep3B
and Huh7 cells, regions stained with antibody to hem-
agglutinin (HA), indicating ... level
increased in Occ
WT
-overexpressing cells, and that
2-APB inhibited the effects of Occ
WT
. Therefore,
the increase in intracellular calcium may explain the
induction of apoptosis and reduction ... findings, our research provides new insights into
the role of exon 9 in the regulation of apoptosis and
invasion in liver cell lines.
A
B
Fig. 7. Effects of occludin shRNA on apoptosis and invasiveness....
... of
copper binding could still allow domain swapping to
occur. It is of interest that a N-terminally truncated pri-
on protein, lacking the copper binding domain is cap-
able of domain swapping and forms ... places, but in particular, stefin B has a number
of histidines in the C-terminus (box in Fig. 1) at sites:
92, 75, 66 and 58. As histidine residues are central to
copper binding in many proteins they ... fibril
formation of this protein is diminished in presence
of Cu
2+
ions. Another interesting finding is that
copper binding gets less strong at pH 5, under fibril
promoting conditions and that a mutant,...
... mutations in the AUH gene. This indi-
cated that AUH, in addition to its RNA binding func-
tion, must play an important role in leucine catabolism.
AUH was overproduced in E. coli and characterized
by ... using
crotonyl-CoA as a substrate [15]. The gene encoding
this bifunctional protein was named AUH (‘AU bind-
ing homolog of enoyl-CoA hydratase’). The RNA-
binding activity of the human protein ... For the
kinetic characterization of AUH described in the work
at hand, AUH was overproduced in Escherichia coli as
a maltose binding protein fusion (MBP-AUH). The
complete AUH gene (producing MBP-AUHp40...
... dynamic
and complex class of vesicles, in which lysosomal cath-
epsins are distributed in a nonrandom manner. Murine
J774 macrophages concentrate cathepsin H in early
endosomes and cathepsin S in ... proline in cystatin F, thus
partially explaining the overall lowered affinity of cyst-
atin F for these enzymes. Proline in the S
2
site is a fea-
ture of human stefin A and cystatins F, S and ... general domain-swapping in the cystatin family
[20]. Although both additional cysteines in cystatin F
at positions 1 and 37 (cystatin C numbering) can form
a disulfide bond [13], the cysteine at position...
... P
i
released min
)1
Æmg
)1
of protein.
Enzyme kinetic study
The K
m
(Michaelis constant) and V
max
(maximum velocity)
values were determined by incubating the diluted recombin-
ant proteins in the standard ... deparaffinized and
dehydrated using a graded series of alcohol and then
rehydrated in NaCl/P
i
. The slides were blocked for 30 min
in 1% H
2
O
2
in NaCl/P
i
containing 10% ethanol to inacti-
vate ... at 26 000 g for 30 min
at 4 °C. The supernatants containing recombinant proteins
of AsPPase were purified using ProBond
TM
resin (Invitro-
gen) under nondenaturing conditions and subsequently
eluted...
... erythrocytes,
and the difference between acetylcholinesterases of kid-
ney and erythrocytes in the extent of binding with the
lectins concanavalin A, Lens culinaris agglutinin
(LCA), and Ricinus communis ... nicotinic receptors [19], amyloid b-peptide and
presenilin-1 [20], neuronal enolase, the scaffold protein
RACK1 and protein kinase C [21], the corepressor
CtBP [22], and Ran-binding protein [23]. ... of
Alzheimer’s disease in the brain in vivo.
Neurobiol Aging doi:10.1016/j.neurobiolaging.2010.04.
028.
26 Vidal CJ (2005) Expression of cholinesterases in brain
and non-brain tumours. Chem Biol Interact 157–158,
227–232.
27...
... resol-
ving cysteine (Cys-S
R
). In typical 2-Cys, S
p
at the N
terminus and S
R
at the C terminus of the protein
belong to different subunits and condense to form an
intersubunit disulfide bond; in ... S
p
peroxidatic cysteine;
X unidentified electron donor; S
R
resolving
cysteine; RSH protein or domain with CXXC
motif (e.g. thioredoxin). In typical 2-Cys, S
p
at the N terminus and S
R
at the C terminus
belong ... protein instability caused
by oxidative stress conditions, are important in deter-
mining the level of Bcp2 protein. Similar results are
observed for genes and related proteins involved in
oxidative...
... a protein
containing a single ankyrin repeat that interacts with
HIV-1 viral protein R (Vpr) and we designated this
protein as Vpr-binding ankyrin repeat protein
(VBARP). This interaction was ... aprotinin, and protease inhibitors leupep-
tin, chymostatin, and pepstatin (1 lgÆmL
)1
; Sigma) as des-
cribed [14] for 15 min in ice. Cell lysates were centrifuged
at 22 000 g for 10 min at ... play an important role
in protein–protein interactions. ANKHD1 proteins are known to possess
multiple ankyrin repeat domains and a single KH domain with no known
function. Using yeast two-hybrid...
... a-actinin, either in direct contact or in a
complex including the two proteins.
In conclusion, our study proves the interaction between
a-actinin and calpain 1 and locates binding motifs within
regions ... Calp1 binding site in
the C-terminal region of a-actinin [56] situates the protease in
the vicinity of titin [57] and CapZ [34], two proteins described
as a-actinin partners in the Z-line and known ... cytoskeletal
proteins [25,51].
Binding interface between Calp 1 and ask was further
located in this study. Calpain binding structures were found
within the 10 kDa C-terminal domain of the a-actinin
molecule....
... listed in the right-hand column of Table 2.1, indicates the mission’s
objectives, and outlines its relevance to the space exploration initiative. Finally, one-page descriptions of
the missions and ... vehicles into ionospheres and atmospheres,
communication within and through planetary ionospheres, and survivability in the radiation environments
of interplanetary space and within planetary ... weather on humans, instrumentation, and
communications and spacecraft systems. To that end, input from space physics observations and
analysis will help in modeling and developing predictive capabilities...
... lean and thin. Body skin is dry
and thin. Face turns pale and eyes sink. The facial
bones become prominent and bones and skin come in
contact due to wasting. Both temporal sink.
Clinical features ... days and is of low grade and does not
have the symptoms of infectious fever like chills,
rigors, thirst, irritability, dryness of tongue and foul
smelling urine.
The patient becomes lean and ... disease, Sil and the
causing agent Ajsame Khabitha (microorganisms).
Symptoms and signs
Pulse is subtle, firm, continuous and weak. Fever is
continuous till morning. Fever is constant and due...
... this
decision-making process in practice: Wheal Jane and South Crofty (Cornwall), Quaking Houses
(Co Durham), Hlobane Colliery (South Africa) and Milluni Tin Mine (Bolivia). In many cases,
particularly ... frequent
contaminants are Fe, Mn, Al and SO
4
, with locally important contributions by other metals /
metalloids including (in order of decreasing frequency) Zn, Cu, As, Ni, Cd and Pb. Remedial ... 1
THE MINE WATER POLLUTION THREAT TO WATER RESOURCES ANDITS
REMEDIATION IN PRACTICE
PAUL L. YOUNGER
Pollution by mine drainage is a major problem in many parts of the world....