cloning expression function structure and immunoreactivities of a sphingomyelinase d from loxosceles adelaida a brazilian brown spider from karstic areas
Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
Ngày tải lên :
06/03/2014, 22:21
... displayed as side chains and located in domain III, domain V and the interface of domains G, III and V (B) Mutation sites in domain III that may affect the FA-binding pocket Mutation sites are ... a Pilatus detector Later, a 1.9 A resolution dataset was collected at beamline ID14-1, ESRF (Grenoble, France), with an ADSC Q210 CCD detector Data were ˚ collected at 100 K and 0.9334 A Data ... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded b-sheet...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
Ngày tải lên :
12/08/2014, 03:20
... TACTTTCTACAACGAGCTTC ACATGATTTGAGTCATCTTC GGGTTTCATATGAAGATCGAGGTGAGAGAA CGGGATCCTTAGATATCATATAGGAACTTGC ATATTCACGACGACTCCGATAGCGGTATCG CACGTCGGCTTCGACTGTAGGTCGACT CACGAGACCAAGTCAATGCACTCAAAGGA GATTCGGGCACTTAAACGTATGAGCCCC ... HQT-OuterRev AAGCCNWSNAARCC CCCCANCCRAARTC GGGTTTCATATGACTATCGGAGCTCGTGAT CGGGATCCCTAGAAGTCATACAAGCATTT TTTTTAAGCTAACACGAGAC TCTCATAGGAGCTGTAATTG TAAAATGGACGATCAGTATC TTATGTTCAGATTTGGACTC TACTTTCTACAACGAGCTTC ... GATTCGGGCACTTAAACGTATGAGCCCC CGTGGACTATCAGACGATCAACCATCC TCGTCCGTCAGTAGCCACGTACAGTATC CACAAAACCAAAACTTCACATCCCATCC CTCACTATGGATTCTCCTAGCGGTGTCG Page 10 of 13 (page number not for citation purposes) BMC Plant...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
Ngày tải lên :
14/03/2014, 23:20
... enzymatic activity of the recombinant AATB3 expressed and purified from E coli was analyzed Native PAGE analysis showed that the wild-type AATB3 had AAT activity when l-aspartate and the a- ketoglutarate ... Shanghai, China) using BSA as the standard A paper chromatography assay for amino acids was also used to detect the activity toward tryptophan The reaction was performed as described above, and a- ketoglutarate ... a- ketoglutarate was used as the amino acceptor; L-Glu, standard L-Glu; L-Try, standard L-Try; 1-3, reaction sample (B) The oxaloacetate was used as the amino acceptor; L-Asp, standard L-Asp; L-Try, standard...
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Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca pptx
Ngày tải lên :
23/03/2014, 21:20
... were removed and added to 1.5 mL of p-hydroxybenzoic acid hydrazide (PAHBAH) reagent [16] and boiled for according to the published procedure The absorbance (A) at 410 nm was read and a reference ... oligosaccharides (XGOs) was calculated from the manufacturer’s data and was found to be 1293 Da This value agreed with the MS analysis of the tamXG digestion products Exhaustive digestion of tamXG ... of acetic acid, mL of p-anisaldehyde, mL of concentrated sulfuric acid) and then heated for h at 95 °C, as described by Chirco & Brown [19] and Jung et al [12] Glucose and xylose oligomer standards...
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Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
Ngày tải lên :
30/03/2014, 13:20
... with a sequence complementary to the 5¢ end of poneratoxin gene and 3¢ end of a signal peptide: 5¢-CAGAAGCGGAA GAAAGCATGCAAAGGCAGA-3¢ (number 2), were used In the second PCR the plasmid pFastBacPx ... GAAGAGAGCCAAGGATCAGAAGCGGAAGAAA CATG-3¢, were used to synthesize the N-terminal fragment of the poneratoxin gene Two others: forward 5¢-GCC GCCCGTGATACAGGCGATCCACGATGCGCAGA GGTAGTAATGAG-3¢ and reverse 5¢-AATTCTCATTA CTACCTCTGCGCATCGTGGATCGCCTGTATCAC ... of 100 averaged laser pulses Aliquots of lL samples and of lL of a saturated solution of a- cyano-4-hydroxycinnamic acid prepared in 50% aqueous acetonitrile/0.3% trifluoroacetic acid (TFA; v/v/v)...
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Báo cáo khoa học: Genomic structure, expression and characterization of a STAT5 homologue from pufferfish (Tetraodon fluviatilis) ppt
Ngày tải lên :
31/03/2014, 07:20
... exon tcccaactag acgtatgtag tctctttaag tatttaacag ccccacacag atgataccag tgcataacag ttttctacag tctgccacag atccttgtag ctctgcacag ctgtccacag ctgtttacag ttttttttag atgtttccag tcacctaaag ccgtgcacag tcctgtccag ... GAGCCTGATTTCTTTGAAATGATGAGC STAT5 mut STAT5 AGATTTCTAGGAATTCAATCC AGATTTAGTTTAATTCAATCC STAT6 CCGCTGTTGCTCAATCGACTTCCCAA GAACA CCGCTGTTGCTCAATCGACTAGCCAA GAACA GCCGTGTAGTTTCTTGGAAATTTCTGG GCCGTGTAGTTTAGATTAAATTTCTGG ... 354 GAA GGC AAG AG GGG CAG CTG TG AGT CAG CTT AAG GAG GCC ACC AAT CGT ATC CAG G AAA TAC CGA CTG CTG CAG TCA TG CTG GTT ACC AG AAC ACA AGG AA TTC AGG AAC ATG TTT CAA GTG AAG TTT GCA GAG CCG CAG...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
Ngày tải lên :
31/03/2014, 08:20
... (Promega, Madison, WI, USA) and [a- 32P]dCTP as the labeled nucleotide ORF analysis was based on the specific codon usage of Streptomyces [25] Deduced amino-acid sequence was compared with the database ... determined from a standard curve of pI and migration distance (cm) of protein standards Enzyme assay and analytical methods E coli BL21(DE3) containing plasmid pNA101 was grown at 37 °C for 12–14 ... recombinant NaoA after Sephadex G75 chromatography; lane 5, purified recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers (phosphorylase b, 97 kDa;...
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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot
Ngày tải lên :
21/02/2014, 15:20
... The backscattered Raman and ROA spectra of disordered poly(L-glutamic acid) (top pair) and poly(L-lysine) (middle pair) in aqueous solution at pH 3.0 and 12.6, respectively, and of native human ... bands from buer salts in the parent Raman spectra are marked with ÔbÕ measurements were made at this reduced pH As the native proteins are already in an unfolded state, such mild acidic conditions ... caseins are largely ÔstructurelessÕ with little extended secondary structure, but later UVCD studies suggested that, although largely Ôrandom coilÕ, aS1- and b-casein may contain » 20% a helix and...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
Ngày tải lên :
07/03/2014, 16:20
... associated to an antiparallel strand (b2) and is surrounded by helices (a1 , a2 , a3 , a7 and a8 ) The second domain consists of helices a4 , a5 and a6 all clustered on the top of the enzyme, as described ... enzymes share a functional catalytic triad made of a catalytic nucleophile serine, associated to a proton carrier histidine and a charge relaying aspartic (or glutamic) acid To further investigate ... 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and 10 A wide The pocket is made by three distinct surface regions: a 10-residue...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
Ngày tải lên :
07/03/2014, 16:20
... used for RT-PCR were: for the I7 OR (5¢-CGTCAAGGAGAAAAAACCCCGGATCT AAAAAATGGAGCGAAGGAACCACAG-3¢) and (5¢-AG CTGCCTGCAGGTCGACTCTAGAGGATCCTAACCAA TTTTGCTGCC-3¢); for OR17-40 (5¢-CGTCAAGGAG AAAAAACCCCGGATCTAAAAAATGGAGCAGAAAC ... AAAAAACCCCGGATCTAAAAAATGGAGCAGAAAC TCATCTCTGAAGAGGATCTG-3¢) and (5¢-GCATG CCTGCAGGTCGACTCTAGAGGATCTCAAGCCAGT GACCGCCTCCC-3¢); for Golf (5¢-GGTACCGCTGCAA TGGGGTGTTTGGGCAAC-3¢) and (5¢-GCGGCCGCCT CAGATCACAAGAGTTCGTACTGC-3¢); ... introducing the c-myc-OR17-40 coding sequence, using primers (5¢-CGTCAAGGAGAAAAAAC CCCGGATCTAAAAAATGGAGCAGAAACTCATCTC TGAAGAGGATCTG-3¢) and (5¢-GCATGCCTGCAGG TCGACTCTAGAGGATCTCAAGCCAGTGACCGCCT...
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Báo cáo khoa học: Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase doc
Ngày tải lên :
16/03/2014, 18:20
... plate This was allowed to dry at room temperature to form cocrystals of protein and matrix BSA was used as an external mass standard Single and double charged peaks arising from BSA were used ... falciparum NF54 IDLVKKYPIVSIEDPFDQDDWENYAKLTAAIGKDVQIVGDDLLVTNPTRITKALEKNACN 351 P falciparum K1 IDLVKKYPIVSIEDPFDQDDWENYAKLTAAIGKDVQIVGDDLLVTNPTRITKALEKNACN 351 P yoelii IELVKKYPIISIEDPFDQDDWENYAKLTEAIGKDVQIVGDDLLVTNPTRIEKALEKKACN ... results of these experiments are presented in this paper Materials and methods Materials Taq DNA polymerase, T4 DNA ligase, endonucleases (KpnI and PstI), 4¢,6¢-diamidinophenylindole (DAPI) and 2,2¢-azinobis(3-ethylbenzo-6-thiazolinesulfonic...
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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
Ngày tải lên :
16/03/2014, 23:20
... arrows indicate predicted b-strands and black lines indicate predicted random coils The four Ca2+-binding domains were boxed; homologous and identical amino acids are indicated by dots and stars, ... sequence of oyster P fucata CaLP and CaM (B) CaMPRED and CaLPPRED represent the predicted secondary structures of CaM and CaLP, respectively Green barrels indicate predicted a- helices, yellow arrows ... CaLP cDNA was obtained by using 5¢- and 3¢-rapid amplification of cDNA ends technique (RACE) To obtain the 3¢-terminal of CaLP cDNA ends, the initial round of PCR reaction was conducted with a...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
Ngày tải lên :
23/03/2014, 09:21
... AY032675 DQ650638 AY206412 AY206413 AF244923 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA At5g40150 NA NA NA NA NA NA NA NA NA NA NA NA At5g05340 NA NA NA NA NA NA NA NA NA Unpublished Unpublished ... transferred to another tube, and Triton X-100 (final concentration of 2%) was added from a 10% stock in STE and well mixed In addition, 400 lL of washed 50% GST beads were also added and agitated ... lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and #PFLF-1 and...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
Ngày tải lên :
23/03/2014, 21:20
... (1977) Analysis of singleand double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange Proc Natl Acad Sci USA 74, 4835–4838 Savonet, V., Maenhaut, C., ... thank here Dr M Spaargaren and Dr J Camonis for their kind gift of cDNAs We want to thank Patricia Cornet for the kind gift of uterine mRNAs and Vanessa Van Vooren for human thyroid RNA preparation ... AIP1/Alix [40] and nematode Caenorhabditis elegans YNK1 [41](Fig 1) Functional and genetic evidences relate Bro1 to components of the yeast PKC1p-MAP kinase cascade [37] and Pal A to pHdependent...
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Báo cáo khoa học: Functional aspects of the solution structure and dynamics of PAF – a highly-stable antifungal protein from Penicillium chrysogenum pdf
Ngày tải lên :
29/03/2014, 23:20
... CGTTCTTAGAAGCGGTGCATTTTCC GTTTGATAACAAGGCTTGCACCAAGG CCTTGGTGCAAGCCTTGTTATCAAAC GAAGTGCACCGCTGATAATAACAAATG CATTTGTTATTATCAGCGGTGCACTTC GTTTGATAACAAGGCTTGCACCGCTG CAGCGGTGCAAGCCTTGTTATCAAAC GGAAAATGCACCGCTTCTAAGAACG ... S2), all amide NH-s and Asn side-chain NH2 groups were clearly resolved and assigned Many amides appear as doublets as a result of large 3JHN,HA couplings ($ Hz) that are characteristic of a dominant ... Oligonucleotide Sequence (5¢- to 3¢) PCR template opafK9Ase opafK9Arev opafK35Ase opafK35Arev opafK38Ase opafK38Arev opafK35,38Ase opafK35,38Arev opafK9Ase opafK9Arev GGAAAATGCACCGCTTCTAAGAACG CGTTCTTAGAAGCGGTGCATTTTCC...
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Báo cáo hóa học: " Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-" pot
Ngày tải lên :
21/06/2014, 19:20
... 5’-GGGCATCACAGACCTGTTAT-3’ 5’-AAYATGGAYATHGAYTGYGA-3’ 5’-RTCDCCCCADATNCCRTA-3’ 5-CCAGAGCACGTTGGCATCAA-3 5-ACCATAGTCGGACTTGACCT-3 5’-GGTCTGCAACAACAAGCTCATC-3’ 5’–GAGTCGATGCCGTCTTGATC-3’ 5’-CGCCATATGAAAACAGCTGCCATT-3 ... activity and stability of chitosanases Chitosanase activity was measured at the pH range of 2.5 to 8.0 for 30 before assayed by standard assay method The residual chitosanase activity was also measured ... signal peptide of 20 amino acid residues (accession number JF950270) The mature CSN has a predicated molecular mass of 24.6 kDa and a deduced pI value of 4.18 Homology analysis of amino acid sequence...
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báo cáo khoa học: "Expression of a quantitative character radius incompletus, temperature effects, and localization of a mobile genetic element Dm-412 in Drosophila melanogaster" pptx
Ngày tải lên :
09/08/2014, 22:22
... used Hybridization was done in x SSC and 10 % ARDUE dextransulfate Hybridization in situ followed the method of GALL & P (1971) A method of building the similarity tree on the data of hybridization ... Drosophila melanogaster lines riC, riSN, riSP, riTI13, and riT149 were used as described above DNA extraction and hybridization in situ The extraction of plasmid DNA, containing the MGE Dm-412 (mdg-2) ... &dquo;C and were cultured at this temperature until the imago stage The age of individuals was counted from the hour of egg laying The generation treated by temperature was considered as F&dquo;...
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The intellectual structure and substance of the knowledge utilization field: A longitudinal author co-citation analysis, 1945 to 2004 pdf
Ngày tải lên :
11/08/2014, 05:21
... and interpretation, drafted the final manuscript, and approved the final submitted manuscript LD conducted data analysis and made major contributions to interpretation of findings, writing, and ... process and final products CW coordinated and conducted all searches, ran all analyses, produced all maps, Additional file Search strategy A complete search strategy used in the Web of Science ... Caplan, and Havelock appear in the third decade and remain in each subsequent decade under the knowledge utilization domain EBM first appears in the 1985 to 1994 decade with three authors, Eddy,...
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báo cáo khoa học: " The intellectual structure and substance of the knowledge utilization field: A longitudinal author co-citation analysis, 1945 to 2004" potx
Ngày tải lên :
11/08/2014, 16:21
... and interpretation, drafted the final manuscript, and approved the final submitted manuscript LD conducted data analysis and made major contributions to interpretation of findings, writing, and ... process and final products CW coordinated and conducted all searches, ran all analyses, produced all maps, Additional file Search strategy A complete search strategy used in the Web of Science ... Caplan, and Havelock appear in the third decade and remain in each subsequent decade under the knowledge utilization domain EBM first appears in the 1985 to 1994 decade with three authors, Eddy,...
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