clinical applications of the polymerase chain reaction pdf
Báo cáo y học: "Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit" pdf
Ngày tải lên :
13/08/2014, 21:21
... detected in one of these tests, then other samples taken from the infection site were analyzed If this second culture test of the suspected infectious site revealed the presence of the same organism, ... for identification of the causative pathogens Table shows the underlying diseases of the patients studied The total number of underlying diseases exceeds the total number of enrolled patients ... from the other sample of the same patient Thus, it was decided that both organisms were pathogens Table summarizes the number of samples in which each of the listed organisms was identified The...
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A COMPARATIVE STUDY OF THE DIAGNOSIS OF PULMONARY TUBERCULOSIS USING CONVENTIONAL TOOLS AND POLYMERASE CHAIN REACTION* pot
Ngày tải lên :
06/03/2014, 04:20
... available for comparison of the performance of the individual tests, an analysis of results was done using a variety of standards Efficiency of microscopy, culture and PCR in terms of sensitivity, specificity, ... of six months after being diagnosed as not having tuberculosis, the PCR profile of the individual had changed markedly, suggesting an increase in the bacterial load in the sputum Only seven of ... available clinical information Radiological data was available from 61 subjects The study was conducted blind The purpose of this study was to determine the efficiency of PCR as compared to other...
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Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
Ngày tải lên :
07/08/2014, 18:20
... primer set of 3' NTR had good results but the primer sets of prM and 5' NTR didn’t Therefore, primers and probe of 3' NTR for the diagnostic purpose of JEV were selected The concentration of primers ... out in a micro reaction tube (Corbett Research, Australia) The reaction mixture for each one tube TaqMan reaction mix consisted of µl of Universal 5X buffer, µl of 10 mM dNTP, µl of enzyme mix ... synthesis of cDNA The RT-mix consisted of µl of 5X Universal buffer, µl of 0.1 µl M DDT, µl of 10 mM dNTP, µl of reverse primer (20 pM), of Superscript reverse transcriptase (50 U/ul), µl of DEPC water...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
Ngày tải lên :
07/08/2014, 18:21
... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... elbaliava gnisu dengised erew stegrat lla rof seborp dna sremirp RCP-TR* emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5 ... eborp/remirp redael fo gningised eht rof ylno detcelfer saw noiger B2 laitrap ot redaeL morf gnirevoc ,revewoh ,niarts 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
Ngày tải lên :
09/08/2014, 01:24
... for the messages of both target gene and β-actin gene in the same sample tube when the intensity of the reporter fluorescent signal reached 10 times the standard deviation of the baseline of fluorescent ... calculated as follows: the ∆CT of each target message was obtained by subtracting the CT of β-actin message from the CT of each target message in the same RNA sample The ∆CT values of the same target ... all the members of the Department of Orthopaedic Surgery, The Jikei University School of Medicine, for the collection of samples 23 24 References R519 Saari H, Kontinen YT: Determination of the...
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Báo cáo y học: " “pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies.”" pptx
Ngày tải lên :
12/08/2014, 02:20
... from the study The mean age of the recipients was 48.5 year and the female to male ratio was 2:3 The age range among recipient males was 23 to 56 and female was 23 to 59 The mean age of the donors ... of them were positive for HCMV by pp65 antigenemia assay Of the seventy six donors, eleven (14.47%) of them were positive for HCMV by real time PCR while one (1.3%) of them was positive for the ... presence of antigenemia in 32/33 patients The HCMV infection observed among twenty six recipients of sub-group II is probably due to the reactivation of the latent virus present in them or other...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
Ngày tải lên :
12/08/2014, 04:20
... performances of the duplex primer/probe and singleplex primer/probe assays in the testing of the same serum sample Obviously, the fluorescence value of the sets of primers/probes is higher than that of the ... http://www.virologyj.com/content/7/1/117 Page of The HCV genome is extremely heterogeneous The reason for this genetic heterogeneity is the high error rate due to the lack of proofreading ability of the RNA-dependent RNA polymerase, ... primer/probe A reaction system B: amplification plot of the HCV sample in the singleplex primer/probe B reaction system C: amplification plot of the HCV sample in the duplex primer/probe A and B reaction...
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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx
Ngày tải lên :
12/08/2014, 04:20
... were generated by employing the successively diluted known copy numbers of pVP3 for real-time PCR reaction under the optimized conditions On the basis of the results of correlation coefficient ... Ten-fold dilutions of the pVP3 plasmid DNA were tested by the established FQ-PCR assay to evaluate the sensitivity of the system, and the detection limit was found to be 2.8 × 101 copies /reaction Comparisons ... detected in all the segments of the organs at 8-h PI The copy numbers of GPV in each tissue reached a peak at 48-72-h PI Numbers of GPV DNA decreased at days, and by days, the level of GPV DNA decreased...
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Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction" pptx
Ngày tải lên :
12/08/2014, 04:21
... to determine the subtypes of dengue virus circulating in the island over the last five years by molecular techniques and so assess the efficacy of this method in the adaptation of the current ... average of 20% of the total number of cases selected per year for analysis Twenty-two patients had unknown dates of onset, however the average duration of onset was 6.88 days with a range of – 90 ... not provide information on the serotype of the virus However, single-step reverse transcriptase polymerase chain reaction (RT-PCR) detection and typing of dengue virus offers a sensitive, specific,...
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Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
Ngày tải lên :
12/08/2014, 18:22
... sequence of the applied enzyme that allows assessing of DNA methylation status of the particular locations throughout the genome The alterations of PCR products in the digested DNA template provide the ... Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) The PCR reaction consisted of DNA sample (genomic or digested template), Taq polymerase enzyme (AmpliTaq® Stoffel fragment, Applied ... marker between leukocytes and the others and D marker between fibroblasts and the others The summary of the individual variations mentioned above is shown in Fig Discussion The AMP PCR is a PCR based...
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Báo cáo y học: "Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection" pptx
Ngày tải lên :
12/08/2014, 23:23
... considered the gold standard for the detection of respiratory viruses, but its limitation is that it can only detect a small number Furthermore, the yield of viral culture depends on the quality of sampling, ... and data analysis, and revised the report NJ participated in the design of the study, directed the acquisition and interpretation of clinical data, and revised the report AvL played a substantial ... central origin of respiratory failure, and overt sepsis at the time of admission The study described here was conducted as part of normal patient care; therefore, according to the Medical Ethical...
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Báo cáo y học: "Bench-to-bedside review: The promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detection" pptx
Ngày tải lên :
13/08/2014, 16:20
... therapy [1] While a number of clinical and technical factors may affect the isolation of the infecting organism [8], the volume of blood sampled is the most critical factor in the detection of ... evaluating the use of SepsiTest™ in a clinical setting Key issues in the application of polymerase chain reaction to the diagnosis and management of sepsis The application of PCR techniques to ... determined by the design of oligonucleotide primers, short pieces of synthesised DNA that bind to either end of the sequence and form the starting point for DNA replication by DNA polymerase For...
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Tài liệu Multimedia Applications of the Wavelet Transform pdf
Ngày tải lên :
19/01/2014, 19:20
... in the expectation that a wellsuited transformation will facilitate the reading, i.e., the analysis of the relevant information Of course, the choice of the transform depends on the nature of the ... reviews the fundamentals of the wavelet theory: We discuss the timefrequency resolution of the wavelet transform and compare it to the common shorttime Fourier transform The multiscale property of the ... of keeping the size of the window xed and lling it with oscillations of different frequencies, he tried the reverse: He kept the number of oscillations within the window constant and varied the...
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Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
Ngày tải lên :
13/03/2014, 18:39
... specific DNA segments • Polymerase Chain Reaction • Requires knowledge of the DNA sequence in the region of interest • As more sequence information becomes available, the uses of PCR expand • With ... primers, and synthesize new DNA: 14 duplex molecules of desired product PCR: make large amounts of a particular sequence • The number of molecules of the DNA fragment between the primers increases ... primers, one can amplify the desired region from even miniscule amounts of DNA • Not limited by the distribution of restriction endonuclease cleavage sites Polymerase chain reaction, cycle Primer...
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Báo cáo Y học: Differential scanning calorimetric study of myosin subfragment 1 with tryptic cleavage at the N-terminal region of the heavy chain pdf
Ngày tải lên :
17/03/2014, 10:20
... result of the present work is that the N-terminal cleavage of the S1 heavy chain completely prevents the changes in the thermal unfolding of S1, i.e a significant increase in the protein thermal ... obtained by addition of 0.5 mM BeCl2 in the presence of mM NaF The formation of the complexes was controlled by measuring the K+-EDTA ATPase activity of the protein The ATPase activity of S1, t-S1, or ... structural changes in the myosin head The main goal of these studies was to understand the mechanism of these changes, i.e the mechanism of transmission of structural changes from the nucleotide- and...
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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
Ngày tải lên :
18/06/2014, 16:20
... apoptosis and the antitumor immune response of the host These immunized macrophages may sensitize the cytotoxic T lymphocytes of the host, and the sensitized T lymphocytes may attack the residual ... found that the maximum value of CEA mRNA in patients without malignancy was 8.6, they therefore set the cutoff value as 9.0 [20] Schuster R et al.[21] also used the maximum value of healthy volunteer ... The reporter dye (6-carboxy-fluorescein: FAM) was covalently attached to the 5’ end of the probe, and the quencher dye (6-carboxy-tetramethyl-rhodamine: TAMRA) was attached to the 3’ end of the...
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Novel Applications of the UWB Technologies Part 1 pdf
Ngày tải lên :
19/06/2014, 14:20
... is the reference signal for one of the four constellation points Then the soft value of Ymk and Ysk is used after deciding the Euclidean distance, as the following: Soft( b2 k ) Re(Ymk ) Soft( ... for the demapper outputs two soft bits being the symbol likelihood The demapping process can be simply the process of outputting real part (I value) and then the imaginary part (Q value) of the ... repetition of the same information (complex number) mapping over the OFDM symbols for the slowest out of the coding schemes In conjunction with the FDS approach, 50 QPSK symbols and 50 of their respective...
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Báo cáo nghiên cứu khoa học: " ỨNG DỤNG KỸ THUẬT PCR (POLYMERASE CHAIN REACTION) ĐỂ PHÁT HIỆN NHIỄM SẮC THỂ PHILADELPHIA TRÊN BỆNH NHÂN UNG THƯ BẠCH CẦU MÃN TÍNH DÒNG HẠT (CHRONIC MYELOID LEUKEMIA )" pptx
Ngày tải lên :
23/07/2014, 00:21
... Results show that the BCR-ABL fusion gene was found in 59 (98,33%) of these 60 blood samples In these 59 positive cases, the b3a2 type has the highest rate of 44,07%, followed by the b2a2 type with ... University of Medicine and Pharmaceutics HCM City SUMMARY Peripheral blood samples from 60 Chronic Myeloid Leukemia patients were tested by RT-PCR (Reverse Trancription -Polymerase Chain Reaction) ... molecular Mechanisms of Leukemia induction to treatment of chronic myelogenous Leukemia.Oncogene 21 (56), (2002), 8547- 59 Sawyers, C.L Chronic myeloid leukemia The New England Journal of Medicine 340(17),...
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Báo cáo khoa học: "Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction" ppsx
Ngày tải lên :
07/08/2014, 18:21
... nim rof C 27 ta noitaziremylop lanoitidda yb dewollof ,nim rof C 27 ta noitaziremylop dna ces 03 rof C 65 ta gnilaenna ,ces 03 rof C 59 ta noitarutaned fo gnitsisnoc hcae ,selcyc 54 yb dewollof ... moor ta rh rof gnidnats retfa ces 02 rof g × 000,41 ta degufirtnec neht saw dna dexetrov ,reffub sisyl ni dednepsus saw )g 2.0( nemiceps laceF C 02− ta derots saw tnatanrepus eht ,nim rof g × 000,21 ... ,siralullecartni L fo noitacifilpma cificeps rof sremirp ehT o o o o o noitcaer RCP dna seditoelcunogilO etartlif lasocum morf AND fo noitcartxe eht rof evoba debircsed sa emas eht saw dedeen gnissecorp...
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