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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx
... from either aperfect shield of site B afforded by bilirubin, or, other-wise, by an alternative conformation of the bilirubin–bilitranslocase complex, totally missed by antibody B.Cyanidin 3-glucoside ... was not stimulated by ATP(data not shown).Immunoblots of carnation petal membranefractionsMembrane proteins from subcellular fractions of carna-tion petals were separated by SDS ⁄ PAGE and ... – University of Trieste) for the immunizationand bleeding of rabbits; to Silvia Zezlina for the affin-ity purification of antibody A from rabbit sera; to DrPaolo Ermacora and Prof Giorgio Honsell...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... purification of HbpR itself and has beenshown previously not to disturb HbpR-mediated tran-scription activation [19]. SDS ⁄ PAGE of the soluble cellextracts of E. coli BL21 (pHB171) showed a protein of 43 ... harvested by centrifu-gation (5 min, 1575 g, at room temperature) and resuspendedin Mops medium [5.5 gặL)1 of Mops free acid, 5.1 gặL)1 of Mops sodium salt, 0.5 g ặ L)1 of NaCl, 1 gặL)1 of NH4Cl,0.06 ... activa-tion of HbpR must proceed differently from that of XylR, a fusion protein between DA-HbpR and theA-domain of XylR was found to be incapable of acti-vating the hbpC promoter (not shown). Hence,...
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... filter.RESULTSBinding of Yb3+to apo-hTfThe thermodynamics of binding of Yb3+to apoTf.Binding of Yb3+to apo-Tf was investigated by addition of aliquots of Yb3+to a solution of apo-TF in 100 ... concentration of Yb-Tf (Fig. 7C). TheFF decreased to half of its original valuewhen 18 mol equiv of Yb-Tf was incubated with cells in thepresence of FITC-Fe2-Tf. Therefore part of the FITC-Fe2-Tf ... NMR spectrum of apo-Tf in the presence of 10 mMbicarbonate was recorded after addition of Yb3+(or Lu3+)Fig. 2. Kinetics of binding of Yb3+to apo-Tf. The final concentration of the protein...
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Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism doc
... instead of Azo (Fig. 1). Clear-cut on–offphotoregulation of DNA transcription was obtainedbecause of the efficient suppression of T7 RNApolymerase (RNAP) binding to the DM-azo in thetrans form. By ... stability of the cis form betweenAzo and DM-azo. (A) Illustration of the reversible photoisomeriza-tion of Azo and thermal isomerization of cis-Azo. (B) Quantitativecalculation of cis-form ... Photoregulation of DNA transcription by usingphotoresponsive T7 promoters and clarification of its mechanismXingguo Liang1, Ryuji Wakuda1, Kenta Fujioka1and Hiroyuki Asanuma1,21 Department of Molecular...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... sequence islocated inside the iron box of desA (nucleotides 7–12 of the iron box) , explaining the regulation of desAexpression by binding of DmdR1 to the iron box. Thesame overlapping was found ... the decarboxylation of lysine by a lysinedecarboxylase encoded by the desA gene [8,9]. Lysinedecarboxylases occur in different Streptomyces speciesand are involved in the utilization of l-lysine ... Production of desferrioxamine was restored by complementation with the whole wild-type desABCD cluster, but not by desA alone, because of a polar effect of the desA gene replacement onexpression of...
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Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx
... study of Pem-GIH inthe shrimp.Biological assay for vitellogenesis-inhibitingactivity of Pem-GIH by dsRNA-mediatedfunctional knockdownTo test whether the knockdown of Pem-GIH expres-sion by ... ratio of Vg to actin transcripts in the GIH-depletedbackground suggested that functional knockdown of Pem-GIH led to the induced expression of Vg in theovary.DiscussionBecause of the lack of ... the5Â-region of Pem-GIH. The negative PCR is indicated by ) ve. The actin transcript of P. monodon was used as an internal control of theamount of RNA template. Each panel shows tissue distribution of...
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Báo cáo khoa học: Characterization of testis-specific serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc
... Characterization and regulation of TSSK3 TSSK3 activation is different from its involvement inthe activation of PKB. Inhibitors of other protein kin-ases, known to be activated by insulin (like p70S6K,p38, ... the release of radioactivity duringthe fifth cycle (data not shown). Substitution of Val toTyr in peptide 2 reconstituted phosphorylation of thispeptide by TSSK3 almost to level of peptide 4 ... insulintreatment of cells may also slightly (twofold) enhancePDK1 kinase activity [33]. Therefore, it is thought thatthe role of PDK1 in the activation of other kinases isgoverned by its cellular...
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Báo cáo khoa học: "Unsupervised Discovery of Generic Relationships Using Pattern Clusters and its Evaluation by Automatically Generated SAT Analogy Questions" pot
... resource itself and how to evaluate its intrinsicquality. In (Davidov and Rappoport, 2008) we showhow to use the resource for a known task of a to-tally different nature, classification of relationshipsbetween ... in related work.6.1 Discovery of Known RelationshipsTo estimate recall of our pattern cluster set, weattempted to estimate whether (at least) a subset of known relationships have corresponding ... clustering methods that as-sign given sets of pairs into several clusters, whereeach cluster corresponds to one of a known set of re-lationship types. Their classification setting is thusvery different...
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Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx
... attack by kallikrein generated by activation of PPK as aconsequense of vessel injury. The activation of FXIIand PPK/HK may start with a local increase in theconcentration of Zn2+as a result of ... although the activity of FXIIageneratedonthecellsbyactivationofFXIIinthepresence of Zn2+was significantly higher than thatgenerated in the absence of Zn2+, the fraction of boundFXII that became ... activation of FXII and thereby the entiresystem [6]. In blood plasma the proteolytic efficiency of theproteinases is attenuated by endogenous inactivation of FXIIa and kallikrein by serine proteinase...
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Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
... degree of accuracy, but only if obtained on the same gel.The density of UCP3 protein (i.e. the intensity of thesignal expressed per mg of proteins) was similar in the SSmitochondria of the ... therelative amounts of UCP3 protein and mRNA in the threemuscles are comparable.Effect of fasting on UCP3 protein expressionWe and others have shown that fasting induces upregula-tion of UCP3 mRNA ... consisted of 2 min of denatur-ing at 95 °C, followed by 30 cycles with 95 °Cdenaturingfor 1 s, 56 °C annealing for 5 s and 72 °C extension for16 s. The fluorescent product was detected at the end of...
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Báo cáo khoa học: Binding of the viral immunogenic octapeptide VSV8 to native glucose-regulated protein Grp94 (gp96) and its inhibition by the physiological ligands ATP and Ca2+ Ming Ying and Torgeir Flatmark pot
... increase in its hydrodynamic radius, upon binding of Ca2+[30].Peptide-binding properties of native rGrp94 and its inhibition by physiological ER ligandsSince the original studies by Srivastava ... lM of VSV8 and a globalRmax(obs) of 27 RUặ(ng proteinặmm)2). (C) D ouble-reciprocal plot of the equilibrium-binding isotherms obtained in the absence of ATP ⁄ Ca2+(d), in the presence of ... properties of native Grp94, inclu-ding its binding parameters. However, interactions of Grp94 with its two related ER lumenal chaperonesGrp78 ⁄ BiP and calreticulin were excluded from thisstudy. By...
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Báo cáo khoa học: Regulation of phospholipid biosynthesis by phosphatidylinositol transfer protein Sec14p and its homologues pot
... condition of high PtdCho turnover, the addition of inositol canconsume part of the PA pool, permitting that partialrepression of INO1 transc ription. Alternatively, the a ddi-tion of inositol ... the inability of Sfh2p todecrease PtdCho turnover to wild-type levels i s responsiblefor the inab ility of S fh2p to complement the INO1regulatory defect caused by the absence of Sec14p, wemeasured ... by the following lines of evidence (Fig. 1), namely that(a) mutations in genes encoding structural enzymes of thecytidine diphosphate choline (CDP-choline) pathway forPtdCho biosynthesis bypass...
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Báo cáo khoa học: Regulation of ascidian Rel by its alternative splice variant pdf
... repre-sentation of the structures of the As-rel genome and mRNAs of As-rel1 and As-rel2. (B) Genome sequence of splicing sites. Note thatAs-rel1 mRNA is generated by splicing at the sites shown by openarrowheads, ... amounts of transfected DNA were kept constant(1.5 lg) by adding an empty vector (pBIND or pCI-neo-Flag vector).The level of activity was normalized on the basis of the level of activity of control ... was verified by Western blotting of the fusion protein.Preparation of the genome and genomic PCRThe genome of the ascidian, H. roretzi, was prepared frommuscles. A 5 mL volume of extraction...
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Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
... panel)are shown by bold lines. The concentration of acetonitrile is shown by a dotted line. Arrows indicate the position of substance X.Fig. 7. Sucrose density-gradient analysis of nucleosome ... transglutaminase.Determination of the DCA-binding sites of histone H2BBecause the carboxamide group of Gln9 of histone H2B isselectively transamidated with the e-amino group of Lys5 orLys12 of histone H4 in ... analyzed by MALDI-TOF-MS, and wasfound to have a relative molecular mass (Mr) of 650.32. ThisMrvalue is 17 units less than the sum of the Mr of DCA(335.50) and that of a tripeptide, Gly–Gln–Lys...
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Báo cáo hóa học: " Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome" pdf
... the root of contemporary strains, suggesting that VP0 may have beenacquired by a more recent ancestor of the group by recom-bination.DiscussionEnteritis is caused by a spectrum of viruses ... of the first stagePCR product, under a touchdown cycling protocol: 95°C,4 min, followed by 10 cycles of 94°C/30s, 65°C, 30s(temperature decrease of 1°C per cycle), 72°C 1 min, fol-lowed by ... CentralPage 1 of 10(page number not for citation purposes)Virology JournalOpen AccessResearchIdentification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full...
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