city and colour the urgency lyrics
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
Ngày tải lên :
18/02/2014, 14:20
... resulted in 55% and 51%
loss of the initial activities of BHLmL2 and BHAb,
respectively. These results strongly suggest that Ser is
involved in the catalytic activity of BHLmL2 and
BHAb and are consistent ... respectively.
The influence of pH on the activities of the BH
enzymes was investigated in the pH range 6–11. The
pH optima were determined to be in the basic range
(pH 8.0–10.0) for BHLmL2 and BHAb ... 6 and
Fig. 4, the Hill equation provided an excellent fit, with
coefficients of determination of 0.992 for BHLmL2
and 0.997 for BHAb. In addition, the fits of the
V = f(S) curves were sigmoidal and...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
Ngày tải lên :
18/02/2014, 16:20
... evaluated by the statistical potential
used in the present study. In the case of the RNA muta-
tions to the SRP complex, the mutated RNA residues
are located in a double-stranded region of RNA, and do
not ... onto the RNA bases nearest the center of
the b-sheet structure of the RRM. We then scored
these sequence-variant structures with the distance-
dependent potential function.
Figure 2 shows the ... score; the blue line represents the rank of these
sequences using the distance-dependent potential. The points in
each colored line are sorted independently by rank; the x-axis is the
sort order. The...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... investigation
of the catalytic mechanism and substrate speci city
and to understand the differences therein for this class
of enzymes. In order to identify the subsites of the
ervatamins and to understand ... both the molecules of the
asymmetric unit runs along the extended backbone of
residues 65–64 and is partly exposed to the solvent
(Fig. 2B). On the other hand, the P3 moiety of E-64 in
each of the ... using the programs cns [58] and detwin and
sfcheck in the CCP4 suite [59] confirmed that the data
were twinned, and allowed us to calculate the twinning
fraction (0.394) (Fig. 6A,B) and the twinning...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
Ngày tải lên :
18/02/2014, 17:20
... binding of the
Sfbgly interactions with the aglycone. Thus, the effect
of the alteration of the glycone structure in the stabil-
ity of the ES
à
complex (DDG
à
) for the wild-type and
mutant ... another important issue in understand-
ing the aglycone speci city is the contribution of the
interactions with the aglycone to the catalysis in
b-glycosidases. Mutational studies with ZmGlu1 and
SbDhr1 ... is that the
interactions with aglycone do not affect the binding of
the glycone within the Sfbgly active site.
This hypothesis was further investigated by deter-
mining the influence on the glycone...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... internal membrane side is
the rate-limiting s tep and not – as proposed for most of the
other electrogenic symporters – the return of the unloaded
transporter to the outside of the membrane [17].
In ... alanine, serine
and proline but also osmolytes such as sarcosine and
betaine, and the
D
-enantiomers of serine and alanine. The
apparent affinities of PAT1 substrates are mainly in the
range of 2–15 ... The apparent K
m
values of
D
-Pro,
D
-Ala and OH-Pro increased only 1.5- and 2.1-fold by
depolarizing the membrane from )120 to )20 mV. On the
other hand, the affinity of b-Ala did not change significantly
within...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
Ngày tải lên :
08/03/2014, 08:20
... 3B). Thus for the SBD tree there is not an
obvious border between the hydrolases and transglycosi-
dases, but rather there may be one between the compact
cluster of Bacillus and Thermoanaerobacter ... 251 CGTase with the SBDs
from the CGTases from Thermococcus sp. B1001, Bacillus
ohbensis,andThermoanaerobacter thermosulfurogenes are
37.3%, 63.8% and 74.0%, respectively. Even for the
acarviose ... supports the idea
that there has been a separate evolution of this domain [30].
This together with the findings of the present study indicates
a separate evolution of the domains C and E compared to
the...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
Ngày tải lên :
16/03/2014, 04:20
... synthesis.
Conclusions
The N-termini of the catalytic domains of the GTFA
and GTFO reuteransucrases are the main determinants
for glucosidic linkage speci city. Within these N-ter-
mini, the ... a)
8
barrel with the three catalytic resi-
dues (Fig. 1C), determine the types and ratios of glu-
cosidic linkages synthesized (Table 1).
The A1
⁄
O1 and A2
⁄
O2 regions within the
N-termini of the catalytic ... sequencing),
both p15-GTFA-dN-RS and p15-GTFO-dN-RS were
digested with XbaI and SalI, SalI and SacI, and SacI and
KpnI, and corresponding fragments were exchanged, yielding
the six constructs p15-GTFA-O1-dN-RS,...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
Ngày tải lên :
16/03/2014, 16:20
... speci city is more
pronounced and the synthetic capacity is, respectively,
increased. In the latter system PA behaves as a typical
transferase [14,15]. The nucleophile or S
1
 [16] specicity of
the ... close to the position found in the
complex with PG [19]. The bond lengths and valence angles
of the nitroaniline moiety were taken from the X-ray data
for the system cyclophilin A and the tripeptide ... described [26].
The other substrates were obtained in dry organic
solvents in the presence of organic base (pyridine,
N-methylmorpholine). The solvents used for the synthesis
of the respective...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... may
influence the shape and volume of the acyl binding pocket
and thereby alter the binding mode and affinity of the
enzyme for PAA and derivatives thereof, while maintaining
the hydrophobicity of the binding ... site.
To test the effect of the mutations on the speci city for
phenylacetylated substrates, the steady-state kinetic param-
eters for the hydrolysis of the chromogenic substrate
NIPAB and the inhibition ... AD and P refer to the acyl donor and
product, respectively. The speci city (k
cat
/K
m
)ofPAforthe
produced antibiotic is in general 10-fold higher than the
speci city for the corresponding acyl...
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Install and Configure the Email Server
Ngày tải lên :
27/08/2012, 09:34
... republished
another one working at company2.com. Their user name used will be
sandra@company1.com and sandra@company2.com. But in SAM, they will both
have the same user name, sandra, so one of them must ... to install, add the domain manually be clicking the
server name in the left pane and then click New domain in the right pane. Remember to
set the properties before you add the domain.
Click ... ‘pop’ the emails out to the clients. It’s
the SMTP server that is communicating with other SMTP servers and receives and sends
emails.
Open Computer Management
Expand Services and Applications,...
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The Art of Writing and Speaking the English Language
Ngày tải lên :
07/11/2012, 15:59
... or
aspirates. The chief difference between b and p is that one has the voice sound and the other has not. Most of
the other consonants also stand in pairs. We may say that the sonant consonant and its ... a hard sound as in the, than, bathe, scythe, etc., and a soft sound as in thin, kith, bath,
Smith, etc. Contrast breathe and breath, lath and lathe; and bath and baths, lath and laths, etc.
S ... c is the same as s, and the hard sound the
same as k. The soft sound of g is the same as j, and the hard sound is the true sound of g as heard in gone, bug,
struggle.
Important Rule. C and G...
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John Wiley and Sons - The Portable MBA in Project Management
Ngày tải lên :
07/02/2013, 09:32
... name on the cover of a book that is the
product of many, many hands. To all those who have given me their time and
trust, I offer my thanks and these acknowledgments:
Larry Alexander and Karen ... has been in the training and consulting fields for 22 years working
with for-profit and nonprofit organizations such as McDonald’s, Land’s End,
the U.S. Navy, and the IRS. Author and editor of ... process and the at-
tributes of the successful change agent.
By the end of Part One, you will understand the case for project
management the relative importance of projects to your firm and what
constitutes...
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- khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản
- khảo sát chương trình đào tạo gắn với các giáo trình cụ thể
- xác định thời lượng học về mặt lí thuyết và thực tế
- tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam
- điều tra đối với đối tượng giảng viên và đối tượng quản lí
- điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1
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- khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu
- nội dung cụ thể cho từng kĩ năng ở từng cấp độ
- xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct
- phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ
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