city and colour the girl lyrics video
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
Ngày tải lên :
18/02/2014, 14:20
... resulted in 55% and 51%
loss of the initial activities of BHLmL2 and BHAb,
respectively. These results strongly suggest that Ser is
involved in the catalytic activity of BHLmL2 and
BHAb and are consistent ... respectively.
The influence of pH on the activities of the BH
enzymes was investigated in the pH range 6–11. The
pH optima were determined to be in the basic range
(pH 8.0–10.0) for BHLmL2 and BHAb ... 6 and
Fig. 4, the Hill equation provided an excellent fit, with
coefficients of determination of 0.992 for BHLmL2
and 0.997 for BHAb. In addition, the fits of the
V = f(S) curves were sigmoidal and...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
Ngày tải lên :
18/02/2014, 16:20
... evaluated by the statistical potential
used in the present study. In the case of the RNA muta-
tions to the SRP complex, the mutated RNA residues
are located in a double-stranded region of RNA, and do
not ... onto the RNA bases nearest the center of
the b-sheet structure of the RRM. We then scored
these sequence-variant structures with the distance-
dependent potential function.
Figure 2 shows the ... score; the blue line represents the rank of these
sequences using the distance-dependent potential. The points in
each colored line are sorted independently by rank; the x-axis is the
sort order. The...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... investigation
of the catalytic mechanism and substrate speci city
and to understand the differences therein for this class
of enzymes. In order to identify the subsites of the
ervatamins and to understand ... both the molecules of the
asymmetric unit runs along the extended backbone of
residues 65–64 and is partly exposed to the solvent
(Fig. 2B). On the other hand, the P3 moiety of E-64 in
each of the ... using the programs cns [58] and detwin and
sfcheck in the CCP4 suite [59] confirmed that the data
were twinned, and allowed us to calculate the twinning
fraction (0.394) (Fig. 6A,B) and the twinning...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
Ngày tải lên :
18/02/2014, 17:20
... binding of the
Sfbgly interactions with the aglycone. Thus, the effect
of the alteration of the glycone structure in the stabil-
ity of the ES
à
complex (DDG
à
) for the wild-type and
mutant ... another important issue in understand-
ing the aglycone speci city is the contribution of the
interactions with the aglycone to the catalysis in
b-glycosidases. Mutational studies with ZmGlu1 and
SbDhr1 ... is that the
interactions with aglycone do not affect the binding of
the glycone within the Sfbgly active site.
This hypothesis was further investigated by deter-
mining the influence on the glycone...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... internal membrane side is
the rate-limiting s tep and not – as proposed for most of the
other electrogenic symporters – the return of the unloaded
transporter to the outside of the membrane [17].
In ... alanine, serine
and proline but also osmolytes such as sarcosine and
betaine, and the
D
-enantiomers of serine and alanine. The
apparent affinities of PAT1 substrates are mainly in the
range of 2–15 ... The apparent K
m
values of
D
-Pro,
D
-Ala and OH-Pro increased only 1.5- and 2.1-fold by
depolarizing the membrane from )120 to )20 mV. On the
other hand, the affinity of b-Ala did not change significantly
within...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
Ngày tải lên :
08/03/2014, 08:20
... 3B). Thus for the SBD tree there is not an
obvious border between the hydrolases and transglycosi-
dases, but rather there may be one between the compact
cluster of Bacillus and Thermoanaerobacter ... 251 CGTase with the SBDs
from the CGTases from Thermococcus sp. B1001, Bacillus
ohbensis,andThermoanaerobacter thermosulfurogenes are
37.3%, 63.8% and 74.0%, respectively. Even for the
acarviose ... supports the idea
that there has been a separate evolution of this domain [30].
This together with the findings of the present study indicates
a separate evolution of the domains C and E compared to
the...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
Ngày tải lên :
16/03/2014, 04:20
... synthesis.
Conclusions
The N-termini of the catalytic domains of the GTFA
and GTFO reuteransucrases are the main determinants
for glucosidic linkage speci city. Within these N-ter-
mini, the ... a)
8
barrel with the three catalytic resi-
dues (Fig. 1C), determine the types and ratios of glu-
cosidic linkages synthesized (Table 1).
The A1
⁄
O1 and A2
⁄
O2 regions within the
N-termini of the catalytic ... sequencing),
both p15-GTFA-dN-RS and p15-GTFO-dN-RS were
digested with XbaI and SalI, SalI and SacI, and SacI and
KpnI, and corresponding fragments were exchanged, yielding
the six constructs p15-GTFA-O1-dN-RS,...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
Ngày tải lên :
16/03/2014, 16:20
... speci city is more
pronounced and the synthetic capacity is, respectively,
increased. In the latter system PA behaves as a typical
transferase [14,15]. The nucleophile or S
1
¢ [16] speci city of
the ... close to the position found in the
complex with PG [19]. The bond lengths and valence angles
of the nitroaniline moiety were taken from the X-ray data
for the system cyclophilin A and the tripeptide ... described [26].
The other substrates were obtained in dry organic
solvents in the presence of organic base (pyridine,
N-methylmorpholine). The solvents used for the synthesis
of the respective...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... may
influence the shape and volume of the acyl binding pocket
and thereby alter the binding mode and affinity of the
enzyme for PAA and derivatives thereof, while maintaining
the hydrophobicity of the binding ... site.
To test the effect of the mutations on the speci city for
phenylacetylated substrates, the steady-state kinetic param-
eters for the hydrolysis of the chromogenic substrate
NIPAB and the inhibition ... interactions
with PAA and shields the binding site from the solvent. A
third phenylalanine, bF57, is located at the bottom of the
hydrophobic cleft. The shortest distance between the side
chain of bF57 and PAA...
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comparing and contrasting the two videos of romeo and juliet
Ngày tải lên :
21/03/2014, 21:59
... sorrowful. Then when Juliet shot herself in the head, it was really dramatic, as you heard the sound
of the shot.
To summarise the two films, all I can say is that I loved them both, because they are ... are so different,
but if I had to choose, I’d choose the Luhrmann version, starring Claire Danes and Leonardo
DiCaprio, just because I loved the ending.
...
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THE FACILITATIVE LEADER IN CITY HALL Reexamining the Scope and Contributions ppt
Ngày tải lên :
22/03/2014, 19:20
... very similar, but the capacity of the organization in which they work and the tools
of leadership and the resources on which they can draw diff er substantially. ey also
fi ll their positions ... delegator/organizer, the mayor helps
the council and manager understand and maintain their roles, including helping
the council members understand their responsibilities. Finally, in the policy initiator ... others to u nderstand and accept the leader’s
point of view by the quality of the ideas presented and by the leader’s eff ectiveness
at persuasion. is approach does not depend on rewards and...
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Commercial Sexual Exploitation of Children in New York City, Volume One: The CSEC Population in New York City: Size, Characteristics, and Needs doc
Ngày tải lên :
24/03/2014, 21:20
... 20% and then they change it. And
then they want 50%, and then 75%, and then they wanna try and take all your money.
Then when you try to leave them, they try to find you, beat the shit outta ... used,
and they [customers] would call and tell us where to meet them at in the City, like
around where Covenant House is. And we’d go and meet ‘em, and do it. And then get
our money and go ... out
of the van and approached them and others who were working the area. He said that the two
young girls looked “like they were about 14 years old.” But he also noted that some of the
girls...
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Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate specificity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx
Ngày tải lên :
31/03/2014, 07:20
... d
Phe-Fe
between the center of the aromatic ring and the TH iron not
exceeding 6.5 A
˚
were included in further analysis. In the
conformation with the lowest total energy (13.5 kcalÆmol
)1
),
the distance ... H-bond
between the amine group of
L
-DOPA and the backbone
oxygen of PAH residue Leu249, and a second H-bond
between the
L
-DOPA carboxyl moiety and the Leu249
nitrogen (Fig. 5). The next favorable ... with
the access of the essential cofactor dioxygen to the catalytic
center, thereby blocking the enzymatic reaction. DA and
L
-DOPA were directly coordinated to the active site iron via
the hydroxyl...
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