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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... resulted in 55% and 51%loss of the initial activities of BHLmL2 and BHAb,respectively. These results strongly suggest that Ser isinvolved in the catalytic activity of BHLmL2 and BHAb and are consistent ... respectively. The influence of pH on the activities of the BHenzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range(pH 8.0–10.0) for BHLmL2 and BHAb ... 6 and Fig. 4, the Hill equation provided an excellent fit, withcoefficients of determination of 0.992 for BHLmL2 and 0.997 for BHAb. In addition, the fits of the V = f(S) curves were sigmoidal and...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
... evaluated by the statistical potentialused in the present study. In the case of the RNA muta-tions to the SRP complex, the mutated RNA residuesare located in a double-stranded region of RNA, and donot ... onto the RNA bases nearest the center of the b-sheet structure of the RRM. We then scoredthese sequence-variant structures with the distance-dependent potential function.Figure 2 shows the ... score; the blue line represents the rank of thesesequences using the distance-dependent potential. The points ineach colored line are sorted independently by rank; the x-axis is the sort order. The...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... investigationof the catalytic mechanism and substrate speci city and to understand the differences therein for this classof enzymes. In order to identify the subsites of the ervatamins and to understand ... both the molecules of the asymmetric unit runs along the extended backbone ofresidues 65–64 and is partly exposed to the solvent(Fig. 2B). On the other hand, the P3 moiety of E-64 ineach of the ... using the programs cns [58] and detwin and sfcheck in the CCP4 suite [59] confirmed that the datawere twinned, and allowed us to calculate the twinningfraction (0.394) (Fig. 6A,B) and the twinning...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... binding of the Sfbgly interactions with the aglycone. Thus, the effectof the alteration of the glycone structure in the stabil-ity of the ESàcomplex (DDGà) for the wild-type and mutant ... another important issue in understand-ing the aglycone speci city is the contribution of the interactions with the aglycone to the catalysis inb-glycosidases. Mutational studies with ZmGlu1 and SbDhr1 ... is that the interactions with aglycone do not affect the binding of the glycone within the Sfbgly active site.This hypothesis was further investigated by deter-mining the influence on the glycone...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... internal membrane side is the rate-limiting s tep and not – as proposed for most of the other electrogenic symporters – the return of the unloadedtransporter to the outside of the membrane [17].In ... alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D-enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are mainly in the range of 2–15 ... The apparent Kmvalues ofD-Pro,D-Ala and OH-Pro increased only 1.5- and 2.1-fold bydepolarizing the membrane from )120 to )20 mV. On the other hand, the affinity of b-Ala did not change significantlywithin...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... 3B). Thus for the SBD tree there is not anobvious border between the hydrolases and transglycosi-dases, but rather there may be one between the compactcluster of Bacillus and Thermoanaerobacter ... 251 CGTase with the SBDsfrom the CGTases from Thermococcus sp. B1001, Bacillusohbensis,andThermoanaerobacter thermosulfurogenes are37.3%, 63.8% and 74.0%, respectively. Even for the acarviose ... supports the ideathat there has been a separate evolution of this domain [30].This together with the findings of the present study indicatesa separate evolution of the domains C and E compared tothe...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
... synthesis.Conclusions The N-termini of the catalytic domains of the GTFA and GTFO reuteransucrases are the main determinantsfor glucosidic linkage speci city. Within these N-ter-mini, the ... a)8barrel with the three catalytic resi-dues (Fig. 1C), determine the types and ratios of glu-cosidic linkages synthesized (Table 1). The A1⁄O1 and A2⁄O2 regions within the N-termini of the catalytic ... sequencing),both p15-GTFA-dN-RS and p15-GTFO-dN-RS weredigested with XbaI and SalI, SalI and SacI, and SacI and KpnI, and corresponding fragments were exchanged, yielding the six constructs p15-GTFA-O1-dN-RS,...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... speci city is morepronounced and the synthetic capacity is, respectively,increased. In the latter system PA behaves as a typicaltransferase [14,15]. The nucleophile or S1¢ [16] speci city of the ... close to the position found in the complex with PG [19]. The bond lengths and valence anglesof the nitroaniline moiety were taken from the X-ray datafor the system cyclophilin A and the tripeptide ... described [26]. The other substrates were obtained in dry organicsolvents in the presence of organic base (pyridine,N-methylmorpholine). The solvents used for the synthesisof the respective...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
... mayinfluence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the hydrophobicity of the binding ... site.To test the effect of the mutations on the speci city forphenylacetylated substrates, the steady-state kinetic param-eters for the hydrolysis of the chromogenic substrateNIPAB and the inhibition ... interactionswith PAA and shields the binding site from the solvent. Athird phenylalanine, bF57, is located at the bottom of the hydrophobic cleft. The shortest distance between the sidechain of bF57 and PAA...
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THE FACILITATIVE LEADER IN CITY HALL Reexamining the Scope and Contributions ppt
... very similar, but the capacity of the organization in which they work and the tools of leadership and the resources on which they can draw diff er substantially. ey also fi ll their positions ... delegator/organizer, the mayor helps the council and manager understand and maintain their roles, including helping the council members understand their responsibilities. Finally, in the policy initiator ... others to u nderstand and accept the leader’s point of view by the quality of the ideas presented and by the leader’s eff ectiveness at persuasion. is approach does not depend on rewards and...
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Commercial Sexual Exploitation of Children in New York City, Volume One: The CSEC Population in New York City: Size, Characteristics, and Needs doc
... 20% and then they change it. And then they want 50%, and then 75%, and then they wanna try and take all your money. Then when you try to leave them, they try to find you, beat the shit outta ... used, and they [customers] would call and tell us where to meet them at in the City, like around where Covenant House is. And we’d go and meet ‘em, and do it. And then get our money and go ... out of the van and approached them and others who were working the area. He said that the two young girls looked “like they were about 14 years old.” But he also noted that some of the girls...
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Install and Configure the Email Server
... republished another one working at company2.com. Their user name used will be sandra@company1.com and sandra@company2.com. But in SAM, they will both have the same user name, sandra, so one of them must ... to install, add the domain manually be clicking the server name in the left pane and then click New domain in the right pane. Remember to set the properties before you add the domain. Click ... ‘pop’ the emails out to the clients. It’s the SMTP server that is communicating with other SMTP servers and receives and sends emails. Open Computer Management Expand Services and Applications,...
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The Art of Writing and Speaking the English Language
... oraspirates. The chief difference between b and p is that one has the voice sound and the other has not. Most of the other consonants also stand in pairs. We may say that the sonant consonant and its ... a hard sound as in the, than, bathe, scythe, etc., and a soft sound as in thin, kith, bath,Smith, etc. Contrast breathe and breath, lath and lathe; and bath and baths, lath and laths, etc.S ... c is the same as s, and the hard sound the same as k. The soft sound of g is the same as j, and the hard sound is the true sound of g as heard in gone, bug,struggle.Important Rule. C and G...
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