chromatography tandem mass spectrometry lc ms ms
KHỐI PHỔ LC MS mass spectrometry LC MS
Ngày tải lên :
24/05/2014, 20:42
... T-Wave thứ hai: cho MS2 TOF: Lựa chọn V mode/ W mode GC -MS Tách GC Xác định MS Có thư viện phổ chuẩn Interface Mass spectrometer acts as a detector for GC LC- MS • Tách LC Xác định MS • Khó khăn: loại ... phun điện ESI (Electrospray Ionization) LC- MS • Tách LC Xác định MS • Khó khăn: loại ảnh hưởng dung môi thành phần pha động; thư viện phổ • Để ghép nối LC MS: • giao diện ion hoá hoá học áp suất ... Ví dụ Ứng dụng LC- MS • Phát tạp chất thuốc tổng hợp, • LC: pha động ACN-amoni ethanoat, C18 • giao diện phun điện • Sắc đồ TIC: ion mẹ rửa giải phút thứ 17,7 pic tạp trước sau • • MS: Chất 8,35...
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Báo cáo y học: "Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry." ppsx
Ngày tải lên :
13/08/2014, 15:21
... mass spectrometry; LC- ESI /MS: liquid chromatography- electrospray mass spectrometry; HPLC-DAD: highperformance liquid chromatography- diode array detection; MS/ MS: tandem mass spectrometry; RDA: ... which are referred to as quasi -MS/ MS /MS ions The comparison of the spectra of quasi -MS/ MS and of quasi -MS/ MS /MS may yield the quasi-third order precursor ions The quasi -MS4 spectra may be obtained ... liquid chromatography- electrospray mass spectrometry (LC- ESI/ MS) method to characterize O-diglycosyl flavanones of Fructus Aurantii (Zhiqiao) and ultra-pressure liquid chromatography (UPLC) retention...
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Identification of metabolites of meisoindigo from interspecies microsomes and in vivo biological fluids in rats by liquid chromatography tandem mass spectrometry
Ngày tải lên :
14/09/2015, 14:08
... myelogenous leukemia HPLC high performance liquid chromatography UPLC ultra performance liquid chromatography UFLC ultra fast liquid chromatography MS mass spectrometry MS/ MS tandem mass spectrometry ESI ... (RIA), gas chromatography coupled with mass spectrometry (GC -MS) , liquid chromatography (LC) coupled with ultraviolet (UV), fluorescence, radioactivity or tandem mass spectrometry (MS/ MS) Among ... incubation……………………………………25 2.2.4 Achiral LC- MS/ MS analysis………………………………………26 2.2.5 Isolation of metabolites by preparative HPLC and MS/ MS /MS 29 2.2.6 Chiral LC- MS/ MS analysis………………………………………29 2.2.7...
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Báo cáo y học: "Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)" pps
Ngày tải lên :
13/08/2014, 15:21
... pressurized liquid extraction and high performance liquid chromatography- electrospray ionization tandem mass spectrometry (HPLC-ESI -MS/ MS) Anal Chim Acta 2006, 567(2):218-228 28 Xie PS, Leung ... mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD -MS) Chinese Medicine 2010 5:4 ... study aims to identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC by using high-performance liquid chromatography coupled with diode array detector and mass spectrometry...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
Ngày tải lên :
05/03/2014, 21:20
... capabilities of the ICP -MS instrument, particularly in the sample introduction and plasma systems Details are given in the following sections: Solvent resistant hardware Many ICP -MS instrument fittings, ... to maintain plasma stability Performance The ICP -MS sample introduction setup for the analysis of volatile organic solvents such as isopropyl alcohol (IPA) involves the use of a lower sample flow ... account, additional interferences may be formed, as illustrated in Table For quadrupole ICP -MS (ICP-QMS) the established and most effective method of reducing polyatomic interferences in high...
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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
Ngày tải lên :
20/06/2014, 00:20
... described in Methods Mass spectrometry A Sciex API 3000 tandem MS system was used for MS- MS detection with an electrospray ion source in the negative ion mode (ESI-) Compound specific mass spectrometer ... detection such as mass spectrometry (MS) in contrast to the mostly applied ELISA analytical methods applied yet For a sensitive detection, including structural information, tandem mass spectrometry ... monitoring) mode was chosen to perform the MS- MS detection MRM mode allows a simultaneous registration of all MS- MS transitions at a scan time of 150 ms for each fragmentation At the used ESI...
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Water quality application of inductively coupled plasma mass spectrometry (ICP MS) part 2 determination of 62 elements
Ngày tải lên :
03/07/2016, 18:45
... coupled plasma mass spectrometry (ICP -MS) for the determination of elements — Part 1: General guidelines and basic principles Terms and definitions For the purposes of this document, the terms and definitions ... inductively coupled plasma mass spectrometry Blackie & Son Ltd., London (1989) [5] JARVIS, K.E GRAY, A.L and HOUK, R.S Handbook of inductively coupled plasma mass spectrometry, Blackie, Glasgow ... parts, under the general title Water quality — Application of inductively coupled plasma mass spectrometry (ICP -MS) : Part 1: General guidelines and basic principles Part 2: Determination of 62 elements...
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Highly sensitive and accurate screening of 40 dyes in soft drinks by liquid chromatography–electrospray tandem mass spectrometry
Ngày tải lên :
25/08/2016, 22:30
... nylon membrane prior to LC- MS/ MS analysis 2.3 Instrumentation LC coupled with electrospray ionization tandem mass spectrometry (ESI -MS/ MS) was used for screening The LC system was Agilent (Palo ... detected This suggested that the HPLCMS /MS method is appropriate for the screening of illegal dyes in foods Conclusion In summary, by combining SPE cleanup and HPLC -MS/ MS, an accurate and highly sensitive ... column (100 × 2.1 mm i.d., 3.0 m) (Welch Materials, Maryland, USA) F Feng et al / J Chromatogr B 879 (2011) 1813–1818 1815 Fig HPLC-ESI -MS/ MS chromatograms from a 40-dye mixed standard solution...
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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
Ngày tải lên :
14/02/2014, 08:20
... to real-time monitoring, tandem mass spectrometry (MSn) helps to identify unknown species, validate proposed intermediates and further understand the reaction mechanisms To demonstrate this, an ... have low proton affinities Rapid Commun Mass Spectrom 2008; 22: 2993–2998 DOI: 10.1002/rcm Monitoring of organic chemical reactions using EESI -MS Figure Mass spectra of the Michael addition reaction ... range (MassLynx 4.0, Waters, Manchester, UK) Collisioninduced dissociation (CID) was performed at a collision energy of 10–25 arbitrary units, as defined by the manufacturer PEA (99%), benzyl alcohol...
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Tandem Mass Spectrometry – Applications and Principles Edited by Jeevan K. Prasain docx
Ngày tải lên :
31/03/2014, 19:20
... purified:
…(Thomson 1921) J J Thomson made this statement about mass spectrometry, but it may be even more apt in describing tandem mass spectrometry As such, besides being the father of mass spectrometry, ... (2000) Tandem mass spectrometry: dissociation of ions by collisional activation Journal of Mass Spectrometry 35(9): 1069-1090 Sleno, L and D A Volmer (2004) Ion activation methods for tandem mass spectrometry ... Introduction Tandem MS is considered as a mass spectrum of a mass spectrum raised to the power n, where n is the number of MS/ MS spectra, obtained per molecular ion When a peptide is analyzed by mass spectrometry, ...
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báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
Ngày tải lên :
12/08/2014, 03:21
... time of flight mass spectrometry; MS/ MS: tandem mass spectrometry; MW: molecular weight; NCBI: National Center for Biotechnology Information; PCR: polymerase chain reaction; RP-HPLC: reverse phase-high ... thermolysin or trypsin and introduced by nanoflow RPHPLC ESI into a QSTAR Pulsar i mass spectrometer (Applied Biosystems, Foster City, CA) MS/ MS spectra generated from each digest were used to interrogate ... gliadin sequences from the cultivar under study Methods for MS/ MS data analysis evolve rapidly Search algorithms that match the MS/ MS spectra of peptides with protein sequence information from...
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Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry" pptx
Ngày tải lên :
13/08/2014, 15:21
... performance liquid chromatography (HPLC) and gas chromatography (GC) have been employed to determine the level of anthraquinones in Radix Polygoni Multiflori [32,33] Using HPLC-DAD and mass spectrometry, ... Figure HPLC fingerprints of R-RPM and its corresponding P-RPM from various sources in China Table MS data of major identified/unknown compounds in the HPLC chromatograms of R-RPM Peak No Mass Spectra ... Radix Polygoni Multiflori; P-RPM: processed Radix Polygoni Multiflori; HPLC: high performance liquid chromatography; MS: mass spectrometry; THSG: 2,3,5,4′-tetrahydroxystilbene-2-O-b-D-glucopyranoside...
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Algorithms for peptide and PTM identification using tandem mass spectrometry
Ngày tải lên :
12/09/2015, 08:18
... spectra data generated by mass spectrometry (MS) or tandem mass spectrometry (MS/ MS) The advance in tandem mass spectrometry (MS/ MS) technology has made highthroughput mass spectra generation possible ... problem, we first define some general terms In tandem mass spectrometry (MS/ MS), a peptide sequence ρ = (a1a2…al) is fragmented into a spectrum S The parent mass of the peptide ρ is given by M = ... there is a directed edge (u,v) (or mass α difference) in the graph Gi( Sα ) BST graph Gi(BST) MST graph Gi(MST) Tag Mass Graph Hi(T) MST Mass Graph Path in Tag H(MST) PiH Special case of Gi(T) in...
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NGHIÊN CỨU ĐỊNH LƯỢNG ĐỘC TỐ SINH HỌC BIỂN ASP TRONG THỦY SẢN VÀ SẢN PHẨM THỦY SẢN BẰNG PHƯƠNG PHÁP SẮC KÝ LỎNG GHÉP KHỐI PHỔ TANDEM LC-MS/MS
Ngày tải lên :
16/04/2013, 19:56
... chuột 10 1.4.2 Phương pháp sắc ký lỏng (LC- UV, LC- DAD, LC- FLD, LC- MS/ MS) 11 1.5 Ưu nhược điểm phương pháp dẫn đến việc sử dụng phương pháp LC- MS/ MS phân tích DA 12 1.5.1 Phương pháp ... diễn đường tuyến tính .48 Hình 28 Kết chạy mẫu nghêu Nam Định LC- MS/ MS HPLC-UV 53 Hình 29 Kết chạy mẫu CRM LC- MS/ MS HPLC-UV 54 v MỞ ĐẦU Theo ước tính Tổ chức nông lương (FAO), tổng kim ... ký lỏng (LC- UV, LC- DAD, LC- FLD, LCMS /MS) Thường mẫu ly trích dung môi hữu làm cách cho qua cột chiết pha rắn (nếu cần) Sau đó, dịch chiết làm hòa tan dịch pha động tiêm vào hệ thống HPLC với đầu...
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Tài liệu Make-up Solvent Considerations for Prep and Semi-Prep LC/MS Mass-Based .... doc
Ngày tải lên :
24/01/2014, 04:20
... flow mass spectro collect Flow Splitter Inlet Make-up Pump Semi-Prep Filter Assembly Figure 1: Single-Injection LC/ MS System Figure 2: Make-up Splitter Allows Solvent Choices Dilution Factor= mass ... (242) shows that unlike DMSO adduct (298)- relative abundance of Na+ adduct increases with cone voltage (note DMSO adduct at 20V) 20V 65V 157 common DMSO solvent ion 2(DMSO) H+ from sample solvent ... employed as the dilution solvent This choice can significantly influence the ionization for LC/ MS target mass ions Solvents such as 1,4 Dioxane can increase the solubility range of a methanol make-up...
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Phương pháp phổ khối lượng (Mass Spectrometry - MS)
Ngày tải lên :
11/04/2016, 16:30
... thiết bị phân tích khối Giao diện kết nối GC vào MS tương đối đơn giản Sơ đồ khối cấu tạo thiết bị GC /MS- MS (kiểu tứ cực chập ba) − Với LC/ MS, đầu LC dòng dung môi pha động đưa trực tiếp toàn dòng ... trình nạp mẫu từ LC vào MS thường phải có giao diện ghép nối phù hợp (đai chuyển, chia dòng, phun sương,…) Một ghép nối LC/ MS cần có đặc tính: Cho phép chuyển mẫu hiệu xác từ LC sang MS mà không làm ... PHÁP HPLC -MS/ MS Thực hành quy trình tối ưu hoá điều kiện sắc ký - khối phổ cho hoạt chất Amlodipin nội chuẩn Tiến hành phân tích mẫu chuẩn amlodipin huyết tương người phương pháp HPLC -MS/ MS (dựng...
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