chromatography and mass spectrometry of complex enzyme digests

Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea ﬂexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

Ngày tải lên : 20/02/2014, 23:20

... resembles a ¨ ¨ right hand: two b-sheets and the a-helix form ﬁngers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a loop between the B6 and B9 strands forms a cord (Fig ... C-terminal b-strand A4 gives additional hydrogen bonding with b-strand A5 and the extension of b-strand B3 interacts with a b-strand B4 BAX also has a longer C-terminal b-strand A4 and a short additional ... between and 12 (Table 6) There is one completely conserved salt bridge between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of the loop between b-strands B7 and A6...

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SAP2000® Linear and Nonlinear Static and Dynamic Analysis and Design of Three-Dimensional Structures

Ngày tải lên : 06/09/2012, 15:56

... and all associated documentation are proprietary and copyrighted products Worldwide rights of ownership rest with Computers and Structures, Inc Unlicensed use of the program or reproduction of ... trademark of Microsoft Corporation Adobe and Acrobat are registered trademarks of Adobe Systems Incorporated DISCLAIMER CONSIDERABLE TIME, EFFORT AND EXPENSE HAVE GONE INTO THE DEVELOPMENT AND DOCUMENTATION ... bridge spans 60 feet, and has a width and height of 12 feet each The supports are rollers at one end, and pins at the other The trusses and cross members are to be constructed of 2L4X4’s, while the...

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SAP2000® Linear and Nonlinear Static and Dynamic Analysis and Design of Three-Dimensional Structures GETTING STARTED doc

Ngày tải lên : 01/04/2014, 03:20

... unmatched in terms of ease -of- use and productivity Creation and modification of the model, execution of the analysis, and checking and optimization of the design, and production of the output are ... well as all of the settings described herein, can be considered to be part of the model Output and Display Definitions The definition of the SAP2000 model and the results of analysis and design ... create and analyze the model The design of steel and aluminum frame members features initial member sizing and iterative optimization The design of concrete frame members includes the calculation of...

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Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

Ngày tải lên : 08/03/2014, 02:20

... factor of 18.8% and an Rfree of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2) The resulting model is depicted in Fig The Ca backbones of the ... [38] and in the CD-degrading TVAII [13], but not in the CD-degrading enzymes ThMA and BaCD nor in the majority of a-amylases Ca-II is located in the loop between a1 and a2 of domain A (Fig 2) and ... 1) All a-helices and b-strands are labeled Fig B-factor plots of the main chains of the two molecules of CDase in the asymmetric unit The averages of molecules A (solid line) and B (broken ˚ ˚...

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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the ﬁlamentous fungus, Talaromyces emersonii ppt

Ngày tải lên : 23/03/2014, 13:20

... mechanism of action Kinetic parameters and enzyme ligand interactions of GH7 enzymes are well characterized [19–21] Genes from this family have been cloned and characterized from a variety of fungal ... space group of the 1Q9H crystal, were found The best solutions had correlation coefﬁcients of 56.1% and 55.3%, and R-factors of 40.0% and 40.9% for 1CEL and 1GPI, respectively Reﬁnement of the models ... Denaturation and refolding of reCBH and enzyme assay reCBH (1 mg) was denatured by incubation in a solution of M urea/0.1 M Tris/HCl, pH 8.0, in the presence of 100 mM dithiothreitol and mM EDTA,...

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Báo cáo Y học: Modeling the three-dimensional structure of H+-ATPase of Neurospora crassa Proposal for a proton pathway from the analysis of internal cavities pptx

Ngày tải lên : 23/03/2014, 21:20

... surface envelope of the enzymes Cavities are colored by atom type (red stands for O, blue for N and yellow for S) beginning of the transmembrane domain The presence of this string of polar cavities ... side-chain oxygens of Glu309 on M4 and of Asn796 and Asp800 on M6 In our PMA1_NEUCR model, these residues correspond to Ile331, Ile332, Val334, and Val336 on M4 and Ala726 and Asp730 on M6 Alanine-scanning ... located between Val289 and Ile293 in the N-terminal end of M3, and Trp756 and Gly757 in the cytoplasmic loop connecting M6 and M7 As shown by mutagenesis in yeast, mutation of Val289 to Phe resulted...

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three dimensional mapping of optical near field of a nanoscale bowtie antenna

Ngày tải lên : 06/05/2014, 08:54

... size of 260 nm × 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its ... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie...

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three dimensional mapping of optical near field of a nanoscale bowtie antenna

Ngày tải lên : 06/05/2014, 08:58

... size of 260 nm × 260 nm and a gap of about 62 nm at the fused silica/aluminum interface and 134 nm at the top of the film The illumination is a λ = 457 nm plane-wave Experimental results and discussions ... The aperture has a size of 260 nm × 260 nm and has a gap of 62 nm (B) SEM image of the NSOM probe The hole on the tip is around 150 nm in diameter (C) Simulation model of bowtie nanoantenna Its ... (full-width at half maximum) of tens of nanometers, and its peak intensity can be orders of magnitude higher than a circularly-shaped aperture with a similar level of field confinement [3] Bowtie...

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Báo cáo toán học: " THE THREE DIMENSIONAL POLYOMINOES OF MINIMAL AREA" docx

Ngày tải lên : 07/08/2014, 06:20

... area |c| of the polyomino c is the number of its unit squares We denote by Cn the set of all the polyominoes of area n The perimeter P (c) of a polyomino c is the number of unit edges of the dual ... sequence S0 , · · · , Sn , · · · of the sets of standard polyominoes is the greatest sequence of subsets of the inﬁnite sequence M0 , · · · , Mn , · · · of the sets of minimal polyominoes enjoying ... side of the parallelepiped) References G.E Andrews, The theory of partitions, Encyclopedia of Mathematics and its Applications, Addison– Wesley, 1976 G Ben Arous and R Cerf, Metastability of the...

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báo cáo khoa học: " Three-dimensional reconstruction of cell nuclei, internalized quantum dots and sites of lipid peroxidation" docx

Ngày tải lên : 11/08/2014, 00:22

... mixture of chloroform and methanol (2:1 [v/v]) After extraction, 0.2 volumes of 0.9% NaCl solution were added and the chloroform-containing phase was collected After evaporation of the chloroform and ... example of 3-D reconstruction of nuclei, of chromatin distribution and of sites of lipid peroxidation in cells undergoing oxidative stress by nanoparticles Chromatin reorganization in cell nuclei and ... in (a) and (b) of Fig Discussion In this study we present a versatile, simple and suitable approach to 3-D reconstruction of intracellular QD location and of changes in nuclear morphology and lipid...

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báo cáo khoa học: "Size-dependent endocytosis of gold nanoparticles studied by three-dimensional mapping of plasmonic scattering images" docx

Ngày tải lên : 11/08/2014, 00:22

... the radius of the nanoparticle, l is the incident wavelength, n is the refractive index of environmental medium and εr and εi are the real and imaginary parts of the dielectric constant of the nanoparticle, ... movement and uptake of AuNPs for 45 nm and 70 nm AuNPs Many 45-nm AuNPs are moved and then uptaken by cells, while many 75-nm-AuNPs only moved to the apical surface of the cells Wang et al Journal of ... the binding energy between ligands and receptors This energy refers to the amount of ligand-receptor interaction and the diffusion kinetics for the recruitment of receptors to the binding site...

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báo cáo khoa học: " The first three-dimensional visualization of a thrombus in transit trapped between the leads of a permanent dual-chamber pacemaker: a case report" docx

Ngày tải lên : 11/08/2014, 02:22

... echocardiography PM, TB and MP performed the transthoracal and transesophageal echocardiography PM, TB, MP and AZ analyzed the images of the offline reconstruction AM and HT have been involved ... leads The technology of an offline 3D TEE reconstruction technique helped us to identify that the mass was trapped between the leads of the PM and defined the origin of the thrombus Although 3D ... [2] Anticoagulation and thrombolysis are known to reduce the size of the thrombi present in the cardiac and pulmonary vascula- Page of ture, but they also increase the risk of fragmentation which...

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Báo cáo y học: "Three dimensional evaluation of posture in standing with the PosturePrint: an intra- and inter-examiner reliability study" potx

Ngày tải lên : 13/08/2014, 14:20

... evaluate head, rib cage, and pelvic posture as three rotations and two translations or five degrees of freedom (DoF) In fact for 11 out of 15 variables (a total of 44 Intra- and Inter-examiner ICCs), ... [13,20,28,29] The definition of the ICC method depended on the assumptions of (a) whether each of examiners, time, and participants was a fixed or random factor and (b) the type of error included (true ... methods of calculating ICCs, Standard Errors of Measurement (SEM), and Differences in Examiner's Measurements for a conservative (ICC2,1) and a more usual (liberal, ICC3,1) analysis of our data...

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IN SITU THREE-DIMENSIONAL RECONSTRUCTION OF MOUSE HEART SYMPATHETIC INNERVATION BY TWO-PHOTON EXCITATION FLUORESCENCE IMAGING

Ngày tải lên : 24/08/2014, 12:57

... 24 Specificity of primary antibodies and signal bleed through 25 Image pre-processing 27 Calculation of Pearson’s coefficient 27 Calculation of Mander’s and Mander’s coefficients ... attributes of the heart muscle The goals of my thesis work were twofold First, development and validation of an assay for imaging sympathetic nerves in the living mouse heart, and, second, use of this ... functional and structural properties of the heart Although in vitro studies demonstrate profound effects of sympathetic innervation on ion handling properties of the myocardium via translational and/ or...

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SAP2000 - Three Dimensional Static and Dynamic Finite Element Analysis and Design of Structures

Ngày tải lên : 11/06/2014, 03:16

... Comprehensive Software for the Finite Element Modeling, Static, Dynamic and Non-Linear Analysis and Design of Structures Introduction Powerful and Integrated Structural Analysis and Desi gn Software ... selection and display tools • On screen assignment of properties, loading and supports Actual section view • SAP2000 • Copy/ Paste to and from spread sheets • Unlimited levels of undo and redo ... orthographic and perspective views • Zooming and panning • Standard viewing angles • SAP2000 • User defined and named views Interactive Modeling Guides and Grids • Accurate dimensioning with guidelines and...

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Numerical modeling of three dimensional water waves and their interaction with structures

Ngày tải lên : 13/09/2015, 20:56

... love and care from you iii Table of Contents Acknowledgements ii Table of Contents iv Summary viii List of Tables x List of Figures xi List of Symbols xviii Introduction 1.1 Background of Water ... pressure p and the volume of fluid (VOF) function F , are defined in the center of the cells, while all vectors, i.e., the x-, y- and z-component of the velocities, gravitational accelerations and external ... the incident wave H0 initial height of the hump (1) Hm Hankel function of the first kind of order m Jm Bessel function of the first kind of order m kn wave number of the n-th mode L wave length Ls...

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Development of three dimensional fibrous structures via electrospinning for applications in scaffold based tissue engineering

Ngày tải lên : 14/09/2015, 08:24

... layer of the periosteum is a 3D layer comprising of highly fibrous tissues and vascular network, hence mimicry of this layer would require 3D integration of cells and scaffold Modifications of the ... that an ideal and universal scaffold for repair and regeneration of various tissues in the body does not exist Instead, due to the diversity and complexity of individual tissues and organs, tailor-made ... pre-determined period of time resulted in tough yet flexible nanofiber sheets (Fig 4B, C) that could be handled for downstream applications Morphology and size distribution of PCL and PCL/Col nanofibers were...

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Fabrication of three dimensional free standing electromagnetic metamaterial structures for therahertz frequencies

Ngày tải lên : 06/10/2015, 20:42

... material parameters ε and µ of the strings and retrieved refractive index of the 1SE sample (bottom picture) [30] The shaded bands represent the left handed and right handed pass bands …………………………………… ... becomes negative and has no wave solution µ ε0 ε>0 and µ>0 ε ε

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Effect of periodic suction on three dimensional flow and heat transfer past a vertical porous plate embedded in a porous medium

Ngày tải lên : 05/09/2013, 14:58

... number of research papers of some International Journals Dr Das is currently acting as the honorary member of editorial board of Indian Journal of Science and Technology and as Referee of AMSE ... the following results of physical interest on the velocity and temperature of the flow field and also on skin friction and the rate of heat transfer at the wall The effect of growing permeability ... the velocity and temperature of the flow field are solved employing perturbation technique and the effects of the flow parameters on the velocity and temperature of the flow field and also on...

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02 three dimensional static and dynamic analysis of structure

Ngày tải lên : 12/01/2014, 21:45

... verify the results of all analyses and assume professional responsibility for the results It is assumed that the reader has an understanding of statics, mechanics of solids, and elementary structural ... and are defined in terms of three numbers: modulus of elasticity E , Poisson’s ratio 1-2 STATIC AND DYNAMIC ANALYSIS ν and coefficient of thermal expansion α In addition, the unit weight w and ... trademark of Computers and Structures, Inc SAP90, SAP2000, SAFE, FLOOR and ETABS are trademarks of Computers and Structures, Inc ISBN 0-923907-00-9 STRUCTURAL ENGINEERING IS THE ART OF USING MATERIALS...

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