chromatographic methods gel permeation
Safe carrying methods
Ngày tải lên :
21/08/2012, 17:56
... section 2 they will be in breach of the law and liable for a
fine of $25 000.
Safe carrying methods
Carrying heavy objects can put a strain on your back, arms, shoulders and knees
because ... that are too heavy for you to lift or carry. In these cases,
you may need to use some of these methods:
• Work with someone to carry the load.
• Use a trolley.
• Use a forklift or trolley jack...
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Thu nhận enzyme pectinase từ sp.niger - tinh sạch bằng phương pháp lọc gel và lọc màng
Ngày tải lên :
15/09/2012, 18:22
... Phân tích chế ph
ẩm enzyme pectinase bằng phương pháp lọc gel Bio Gel P 30
Dung dịch enzyme xử lý theo mục 2.5, kết quả sau khi lọc gel với tốc độ chảy qua cột
30ml/giờ thu được 2 peak chính ... hiệu suất cao hơn nên chọn cột lọc gel và kích thước màng
lọc phù hợp với từng loại enzyme.
PURIFICATION OF PECTINASE FROM ASP.NIGER BY THE
TECHNIQUES OF GEL FILTRATION AND CROSS-FLOW MEMBRANE ... inducer obtained the highest activity after 48 hours. Separating enzyme
on a gel filtration column packed with Bio gel P 30, which showed that one peak contained
activity of pectinase, and with...
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- 1.6K
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MOB Subject 5 –Access methods Static and dynamic access techniques
Ngày tải lên :
17/09/2012, 09:13
... makes that it
is largely used), and is given by:
Master of Computer Science 1 - MOB Mobile Internet and Surrounding
1/12 Baey, Fladenmuller – Subject 5
MOB Subject 5 –Access methods
Static and...
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Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
Ngày tải lên :
23/09/2012, 19:25
... centrifuged to separate the gel from the sol phase, allowing the
identification of the gel- forming mucins. Repeated extraction of the gel phase solubi-
lizes the “soluble” gel- forming species, leaving ... a better separation between mucins
Isolation of Large Gel- Forming Mucins 3
3
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... Biochem. Mol.
Biol. 27, 5792.
Methods in Molecular Biology
TM
HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA...
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Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
Ngày tải lên :
23/09/2012, 19:25
... nonsulfated mucins remain
unstained.
Histological Methods for Detection of Mucin 29
29
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... tract.
Histological Methods for Detection of Mucin 39
3. Alternatively, prepared adhesive slides such as Superfrost Plus
®
/(Menzel-Gläser,
Braunschweig, Germany) may be purchased.
4. There are many other methods ... A., and Katsuyama, T. (1997) Histochem-
istry of the surface mucous gel layer of the human colon. Gut 40, 782–789.
Histological Methods for Detection of Mucin 35
10. Antigen retrieval solutions:...
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Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
Ngày tải lên :
23/09/2012, 19:25
... Histologically-based methods for detection of mucin,
in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana,
Totowa, NJ.
2. Davies, R., Carlstedt, I. (2000) Isolation of large gel- forming ... separation. Polyacrylamide gels of
a percentage that will not allow migration of high M
r
mucins even into the stacking gels
are often used. Even at the lower limit of polyacrylamide gel formation (3%) ... Mucins can be detected by chemical methods within acrylamide
Detection and Quantitation of Mucins 45
45
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited...
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Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
Ngày tải lên :
23/09/2012, 19:25
... unfixed mucosa (3). The first two of these methods also measure the
GI Adherent Mucous Gel Barrier 59
unstirred aqueous layer beyond the adherent mucous gel surface, the extent of which
depends ... mounted in a water-soluble gelatin gel. Values
for mucous thickness over the rat gastric mucosa by this method give a mean of
147 µm, which approximates that of the adherent gel remaining after suction ... provided by wrapping them in a 1–cm
2
sand-
GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield...
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Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
Ngày tải lên :
23/09/2012, 19:25
... apparatus to measure radioactivity in flat materials
(gels or filter paper) by autoradiography. It calculates the amount of radioactivity in a
band on gel or on a dot on filter paper, taking into account ... PhosphorImager as au/A
260nm
.
Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Trizol RNA isolation solution (Gibco/BRL, Gaithersburg, MD).
10. Agarose gel electrophoresis apparatus, and 0.8% (w/v) agarose gels.
11. Radiolabeled, homologous cDNA or cRNA probe to quantify the...
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Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
Ngày tải lên :
23/09/2012, 19:26
... × 10
6
) of the gel- forming
mucins in particular, there are few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... perchlorate.
2.3.2. Agarose Gel Electrophoresis
1. Horizontal electrophoresis apparatus, e.g., Bio-Rad DNA subcell Model 96 (25 × 15 cm
gels) and the Bio-Rad subcell (15 × 15 cm gels).
2. Agarose (ultrapure).
3. ... culture, focusing primarily on those mucins
involved in gel formation. At present, the mucins MUC2, MUC5AC, MUC5B, and
MUC6 are known to be gel- forming molecules (1–4). These mucins share common
features...
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Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
Ngày tải lên :
23/09/2012, 19:26
... Subheading 2.3.1.
3. Methods
3.1. Extraction and Purification
For a detailed description, see Chapter 1.
3.2. Rate-Zonal Centrifugation
Because of their extreme size, most gel chromatographic media ... (see Note 5).
Heterogeneity and Size Distribution of Gel- Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield ... biologi-
cal property of gel- forming mucins and a key comparative characteristic when studying
the change of mucous properties through the course of disease.
This chapter describes only methods for assessing...
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Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
Ngày tải lên :
23/09/2012, 19:26
... code as defined in Fig. 1.
Amino Acid Analysis of Mucins 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... desalting column), or the mucin can be dot-spotted onto
polyvinylidene difluoride (PVDF) membrane, or gel separated by electrophoresis and
electroblotted onto PVDF. Note that mucins are often difficult ... Mobile phase B: 15% (v/v) methanol in water.
6. Mobile phase C: 90% (v/v) acetonitrile in water.
3. Methods
3.1. Sample Preparation and Hydrolysis
1. Add an aliquot of a solution of a sample of mucin...
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Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
Ngày tải lên :
23/09/2012, 19:26
... Sequencing Glycopeptides 121
121
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for the
separation of PTH-glycosylated amino acids on a modified Beckman LF3600
sequenator (12). These methods were developed for two reasons: ... with
high molecular weight, heavily glycosylated domains such as those found in mucins.
The two methods for determining the sites of glycosylation in proteins are mass
spectrometry and Edman sequencing....
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Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf
Ngày tải lên :
23/09/2012, 19:26
... R
Trityl C
Synthetic Peptides for Antimucin Antibodies 129
129
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... than peptide alone. Glyco-
peptides can be produced by both chemical (12) and enzymatic (13,14) methods. These
reagents have been of value in assessing the contribution of O-linked N-acetyl-
galactosamine ... cannot be controlled or simply that short peptides do not adhere
well to polystyrene plates. Several methods of peptide modification have been utilized
to overcome these problems. One such procedure...
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Glycoprotein methods protocols - biotechnology 048-9-143-155.pdf
Ngày tải lên :
23/09/2012, 19:26
... Blot Analysis
1. Tris-glycine polyacrylamide gels (precast) (Novex, San Diego, CA).
2. Gel electrophoresis apparatus (Novex Xcell II Mini Cell).
3. Gel running buffer: 25 mM Tris, 192 mM glycine, ... 100 min, or until the bromophenol blue dye reaches the bot-
tom of the gel.
5. Transfer the separated proteins from the gel to a nitrocellulose membrane at a constant
voltage of 100 V for 1 h ... on
polyacrylamide gels. The percentage of polyacrylamide used will vary with the size of
the expected product and the desired separation. Most experiments in the present study
were performed using 8% gels.
1....
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Glycoprotein methods protocols - biotechnology 048-9-159-180.pdf
Ngày tải lên :
23/09/2012, 19:26
... can also occur (Fig. 1) (1,2). Most of the substituents, largely O-acetyl groups
are quite labile during acid or alkaline hydrolysis methods generally utilized for the
release of monosaccharides. ... (Kdn), respectively. Substituents of the hydroxyl groups present on
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... a suitable camera system.
1. Place the gels on a UV transilluminator with maximum emissions at wavelengths of ei-
ther 254 or 365 nm.
2. Photograph the gels through a yellow filter.
Monosaccharide...
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