characterization of pegylated biopharmaceutical products by lc ms and lc ms ms
Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
Ngày tải lên :
19/02/2014, 12:20
... four types, -GlcN-GlcN-, -GlcN-GlcNAc-, -GlcNAc-GlcNand -GlcNAc-GlcNAc-, of which the first is the major type and the last one results from the heterogeneous de-Nacetylation Formation of chito-oligomers ... weight of GlcNAc [24] Further characterization of chitosan and the depolymerization products Further characterization of chitosan and the depolymerization products was performed using a variety of ... action of pronase on -GlcNAc-GlcN-linkage resulting in the products (LMWC and oligomers) with GlcNAc at reducing ends In addition, the presence of GlcNAc in Fraction II and the absence of GlcN in...
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Báo cáo Y học: Characterization of the exopolysaccharide produced by Streptococcus thermophilus 8S containing an open chain nononic acid doc
Ngày tải lên :
08/03/2014, 09:20
... neutralization and removal of boric acid by coevaporation with methanol, the mixture of partially methylated alditols was acetylated with acetic anhydride (3 h, 120 °C), and analyzed by GLC and GLC–EIMS ... composition of Gal, Rib and Glc in the molar ratio of : : (GLC analysis), the deamination product of GalpNAc (2,5-anhydro-Tal-ol-1-d) in a molar ratio of in terms of peak areas compared to Glc, Fig ... spectrum (mixing time 300 ms) of n-EPS, recorded in D2O at 64 °C Diagonal peaks of the anomeric protons, of H-4 of residues A and C, H5 of residue E, and H-3¢a,3¢b,9¢ of residue D are indicated...
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synthesis and characterization of wo3 nanostructures prepared by an aged-hydrothermal method
Ngày tải lên :
20/03/2014, 13:08
... temperature by using ammonium metatungstate as tungsten source and without the presence of additives Experimental 2.1 Synthesis of WO3 Nanostructures WO3 nanostructures were synthesized by using ... step characteristic of mesoporous materials above the relative pressure (P/Po) of 0.4 and specific surface area (SBET) values between 34 and 35 m2 (Table 2) The formation of a mesoporous material ... to the formation of nanostructures in a wide range of thicknesses, most of which had shown an irregular shape The oxygen (O) and tungsten (W) atomic contents were determined by Energy Dispersive...
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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
Ngày tải lên :
12/08/2014, 03:21
... peach and Arabidopsis Figure selected accuracy of the predicted expression patterns of Evaluation of the genes by RT-PCR Evaluation of the accuracy of the predicted expression patterns of selected ... of Ppxero2) of 93°C for 30 sec, 72°C for 15 min, followed by 20 cycles (30 cycles in the case of Ppxero2) of 93°C for 30 sec, 67°C for 15 The amplified products were cloned in pGEM-T vector and ... mediated by the interaction between promoters containing these types of cis-elements and orthologous transcription factors However, the identification and functional characterization of these types of...
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Identification and characterization of conserved regulatory elements by comparative genomics
Ngày tải lên :
11/09/2015, 16:02
... patterns directed by CNEs 1, 2, and 5…………… 102 5.4.3 Summary of the regulatory potential of mouse Foxb1 CNEs…….107 v 5.4.4 Conservation of regulation of Foxb1 between fugu and mouse….108 5.5 ... sequence of the human genome, and to identify and characterize all functional elements which would lead to a more complete understanding of the structure, function and evolutionary history of the ... levels of expression and anterior and posterior boundaries of expression on the anterior-posterior axis and this temporal delay in Hoxc8 expression was sufficient to produce phenocopies of many of...
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Characterization of enamel diffusion modulated by er YAG laser
Ngày tải lên :
12/09/2015, 09:55
... level of absorption of the particular wavelength by tissue The amount of absorbed energy depends on the type and composition of tissues, characteristics of the laser used and modes of emission and ... in that of old teeth (Linden, 1968, Byers and Lin, 2003) The flow of the fluid is higher in the peripheral part of the prism and along the prism than central and perpendicular to the prisms indicating ... prism and interprismatic zones in terms of chemical composition since both contain 86% to 88% by volume of crystallites (Angmar-Mansson, 1970) The angle between crystals of two adjacent prisms can...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
Ngày tải lên :
20/02/2014, 23:20
... included in the calculated average values of uorescence lifetimes ặsDổ and ặsDAổ The values for average lifetimes of Trp85 in the absence and presence of acceptor and efciency of energy transfer ... change in Trp85 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used ... change in Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
Ngày tải lên :
21/02/2014, 00:20
... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... suggests that the bands at approximately 110 and 37 kDa correspond to strongly attached dimers of both NrfA (a2) and NrfH (b2), respectively The presence of dithiothreitol (Fig 1A, lanes and 2) did not...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
Ngày tải lên :
07/03/2014, 12:20
... suggested by its ubiquitous location in the membrane and in the cytoplasm [22], by its constitutive level [21] and by the long half-life (2 h) of the mRNA [23] The dismutation of O2 ) by SOD develops ... (A), paraquat (B) and tert-butyl hydroperoxide (C) Twenty micrograms of cytoplasmic proteins extracted from nonexposed and exposed culture for 30, 60 and 120 were analysed by western blot with ... range of concentrations (0– 100 lgÆmL)1) (B) Effect on protection against DNA cleavage of the replacement of Cys49 of rBcp2 with serine Lane 1, pUC19; lane 2, pUC19 and 10 mM DTT; lane 3, pUC19 and...
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Best Practices in Social Media / Summary of Findings from the Second Comprehensive Study of Social Media Use by Schools, Colleges and Universities docx
Ngày tải lên :
08/03/2014, 19:20
... measures, such as anecdotal evidence and focus groups, and quantitative measures, such as counts of usage and surveys, used in your evaluation of the success of your social media efforts? (Skip ... 31% 27% 16% 8% 2.6 Lack of IT resources 19% 32% 26% 15% 8% 2.6 Concerns about loss of control over content and tone of postings by others 14% 36% 30% 15% 5% 2.6 Lack of institutional clarity ... success of your unit's use of social media, in terms of achieving its goals? Not at all successful Not very successful Somewhat successful Very successful A model for successful use of social...
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Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
Ngày tải lên :
08/03/2014, 23:20
... R3 (PerSeptive Biosystems, Framingham MA, USA) tip, and a sample of the flowthrough carrying the glycans was drop-dialysed and analysed by MALDI-TOF MS RESULTS Isolation of cysteine proteinase ... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... samples of the kininogens were subjected to N-glycan liberation by N-glycosidase F The released glycans were isolated by gel filtration and N-glycan pools were analysed by MALDI-TOF MS The spectrum of...
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Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redefinition of the I-conotoxin superfamily pot
Ngày tải lên :
16/03/2014, 22:20
... presence of reduced and oxidized glutathione with a yield of 70%, and purified to homogeneity by RP-HPLC The identities of the peptides were confirmed by MALDI MS, and the molecular masses of the synthetic ... to d-Phe44 of r11a, i.e d-Phe at position 44 of r11b and HPLC co-injections of each of the two synthetic variants of r11c conotoxin, [l-Leu42]r11c and [d-Leu42]r11c with the natural form were ... sequences of conotoxins r11b and r11c have d-Phe and d-Leu at positions 44 and 42, respectively Henceforth, we will refer to the d version of the peptides simply as r11b and r11c, and the all-l...
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Báo cáo khoa học: Signalling and regulation of collagen I synthesis by ET-1 and TGF-b1 doc
Ngày tải lên :
23/03/2014, 11:20
... possible DAG donors PLC-b, PC-PLC and PLD PLCb, a downstream effector of the Gaq [44] as well as of the bc subunit of Gai [45] was inhibited specifically by U73122 [46] Inhibiting PLC-b by U73122 did ... involvement of the PC-phospholipases We used D609 to specifically inhibit PC-PLC [46], and neomycin [47–49] and N-butanol [50–52] as specific inhibitors of PLD The inhibition of PC-PLC by D609 (Fig 5B) and ... TCA-precipitated and separated by 6% SDS ⁄ PAGE (A) PLCb inhibition by U73122 (10 lM) (B) PC-PLC inhibition by D609 (185 lM) (C) PLD inhibition by neomycin (500 lM) Two specific collagen I bands representing...
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Báo cáo sinh học: " Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes" docx
Ngày tải lên :
18/06/2014, 18:20
... independent series of serial passages (at MOI of 0.02); P1 and P2 were analyzed by RT-PCR and flow citometry at passages and 10 and are represented in all panels as 5P1, 10P1, 5P2 and 10P2 In these ... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... y-axis) and of EGFP by direct detection of its fluorescence (EGFP; x-axis) The controls consisted of cells infected with no virus (control) and the parental virus (YF17D/E200T3) Cells infected by...
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Báo cáo hóa học: " Quality of Life as reported by school children and their parents: a cross-sectional survey" docx
Ngày tải lên :
18/06/2014, 22:20
... translation of the child and parent versions of the KINDL and the ILC The following hypotheses were tested in respect of child and parent reports of QoL in a representative sample of Norwegian ... results of an analysis of unconditional random effects showed that only 3.6% of the total variance of the ILC LQ0-28 scores and 6.5% of the total KINDL Total QoL scores could be explained by differences ... mother and son, father and daughter and father and son reports on the ILC and KINDL were significant, but small and similar (range r = 0.25 to 0.31, and 0.26 to 0.39, on the ILC and KINDL, respectively)...
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báo cáo hóa học: " Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9" docx
Ngày tải lên :
19/06/2014, 22:20
... systemic elevation of sPLA2s are associated with most forms of inflammation [1-5] The secreted isoforms are part of a growing family of PLA2 enzymes whose activity leads to the production of several ... in models of neurodegenerative disease Neurodegeneration and PLA2 isoforms The survival-promoting and anti-inflammatory effects of CHEC-9 are most readily explained by the inhibition of PLA2 activity, ... Identification of inhibitor properties The characteristics of CHEC-9 inhibition were determined using both classical characterization of inhibitor types, i.e., the direction of changes in Km and Vmax, and...
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Báo cáo hóa học: " Construction and characterization of recombinant flaviviruses bearing insertions between E and NS1 genes" pdf
Ngày tải lên :
20/06/2014, 01:20
... independent series of serial passages (at MOI of 0.02); P1 and P2 were analyzed by RT-PCR and flow citometry at passages and 10 and are represented in all panels as 5P1, 10P1, 5P2 and 10P2 In these ... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... y-axis) and of EGFP by direct detection of its fluorescence (EGFP; x-axis) The controls consisted of cells infected with no virus (control) and the parental virus (YF17D/E200T3) Cells infected by...
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báo cáo hóa học:" Women''''s quality of life is decreased by acute cystitis and antibiotic adverse effects associated with treatment" pptx
Ngày tải lên :
20/06/2014, 15:20
... 2) Of all organisms isolated, resistance to TMP/SMZ, ciprofloxacin and nitrofurantoin were 7%, 1% and 8%, respectively For E coli the resistance rates were 7%, 0% and 4% for TMP/SMX, ciprofloxacin ... ciprofloxacin and nitrofurantoin, respectively Clinical outcomes Clinical cure rates did not differ by treatment with 88, 82 and 87% of patients treated with TMP/SMX, ciprofloxacin and nitrofurantoin, ... state of Iowa, both affiliated with the University of Iowa College of Medicine and College of Pharmacy Women aged 18 to 65 were eligible for inclusion in the study if they had clinical symptoms of...
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Báo cáo hóa học: " Nanoscale chemical and structural study of Cobased FEBID structures by STEM-EELS and HRTEM" pptx
Ngày tải lên :
20/06/2014, 22:20
... quantification of the relative amounts of each element, with a collection angle of 24 mrad and a convergence angle of 7.5 mrad Both types of experiments had an acquisition time of 0.8 s/spectrum Results and ... Comparison of the EELS spectra Comparison of the EELS spectra of the Co L2,3 edge (at an energy of 779 eV) for deposits and 2, and references for metallic cobalt and cobalt (II) spectrum, and the ... first estimated by means of the intensity ratio of the L2 and L3 peaks The reference values of I(L2)/I(L3), 0.31 for metallic cobalt and 0.27 for cobalt oxide [CoO] [15], were calculated using...
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