... relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... the amount of glutamate formed, 0.3 mL of the sample was incubated in mL of 50 mM Tris/ HCl, pH 8.0, containing mM EDTA, 0.5 mM APAD and units of glutamate dehydrogenase at 37 °C for 90 After ... centrifugation (14 000 g) for min, the absorbance of the supernatant was read at 363 nm The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lM range of glutamate with a molar...
... Framework of Traffic Source Identification 3.1 Problem Statement 3.2 Components of the Source Identification Model 3.3 Phases of Operations 3.4 Classification ofSource ... developments of web traffic source identifications They serve for the comparative analysis in our research We lay out a framework for traffic source identification in Chapter It classifies the domain of traffic source ... timing information 34 Framework of Traffic Source Identification Applications of Traffic Source Identification Traffic analysis to identify traffic sources or communication paths has a wide range of applications,...
... widely used by the ECR community for the extraction of singly charged ion beams The ‘2D Poisson’ software package has been used in the present work for simulation of the magnetic field pattern using ... conventional ECR ion source uses the principle of adiabatic invariance for mirror reflection, and high magnetic field mode of operation to successfully generate and confine plasma Design of the electromagnet ... described here offers a continuous control of the axial magnetic field, giving tuning capability The fine tuning the magnetic field is very important for the stability of an ECR plasma source, as any...
... could bring to information retrieval, information extraction, digital libraries and so forth, automatic identificationof text types and genres could be particularly useful for problems that ... genre of web pages using the triple 701 This study has the practical aim of finding out whether feature sets for genre identification need to be changed or updated because of ... Biber, 1988; Lee, 2001) Form is a more heterogeneous attribute Form can refer to linguistic form and to the shape (layout etc.) From an automatic point of view, linguistic form is represented by...
... latest refined assays and their utility are provided The use of the CODEHOP methodology for the analysis of larger regions of viral genomes is presented along with the general application of this ... γ1 branch of the phylogenetic tree shown in Figure The identificationof DNA polymerases from two types of EBV-like lymphocryptoviruses corroborates previous reports of the existence of two closely ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24...
... University of Chicago Argonne, LLC, for the United States Department of Energy Office of Biological and Environmental Research under Contract DE–AC02–06CH11357 I am also thankful to the NIH for its ... and the long distances Thanks for being there for me v Abstract Bibek Parajuli IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATIONOF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 ... Characterizationof CB29 binding .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterizationof CB7 binding 123 D Probing CB7 binding of ALDH3A1 site...
... UCSC University of California at Santa Cruz 10 Chapter 1: INTRODUCTION One of the key paradigms of development is the regulation of the genome in a precise and synchronized manner to form a highly ... position of the footprint is read of the gel in relation to the labeled end of the probe The advantage of this method is that single base pair resolution of binding sites can be obtained One of the ... provide additional information on the possibility of a DNA element having a role in cis-regulation A study in 2004 enforced a threshold score of 60% for the maximum possible score for a TFBS and also...
... A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 ACKNOWLEDGEMENT First of all, I would like ... Associate Professor Koh Dow Rhoon, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, for his guidance I am also very grateful to Dr Tin Soe Kyaw for his ... supervisor Associate Professor Fong Kok Yong, Chairman of Division of Medicine, senior consultant, Department of Rheumatology and Immunology in Singapore General Hospital, for his invaluable supervision,...
... advantages of baculovirus include the ease of construction of recombinant viral vector, simple procedure for purification of large volumes of high titre viruses and the virtual absence of cytotoxicity ... characterizes the use of the IFI30 promoter for transcriptional targeting in glioma and paves way for further research in the use of this promoter for in-vivo therapy VI List of Tables Table 2.1- ... gliomas” The prognosis for patients with glioma is often very poor (only ~2% of patients aged 65 years or older and only 30% of those under the age of 45 years at GM diagnosis survive for years or more)...
... inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase the risk of side effects of the statins [3,7].Therefore the developments of novel pharmaceutical formulations ... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... hematocrite of washed erythrocytes was adjusted by PBS to 45% In ml eppendorff tubes, 400 µl of suspension are added to 400 µl of PBS containing the known concentration of the drug and 2.5 mmol of ATP,...
... performance of PVDF-SPS composite membrane should be done The overall findings suggest that the PVDF-SPS membrane offers the potential for improving the performance of the DMFCs, this kind of ... active energy (Ea) of the membranes were calculated, 18.58 kJ.mol−1 for Nafion-117 and 20.05 kJ.mol−1 for PVDF-SPS membrane, respectively Ea of the PVDFSPS membrane is close to that of the Nafion-117 ... hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of PS and SPS polymers,...
... breakdown of lignocellulosic biomass for the establishment of a robust and cost-efficient process for production of cellulosic ethanol Acknowledgements Financial support by the Center for Bioprocessing ... Results and discussion 3.1 Compost characterization A summary of the YWC characteristics is shown in Table All compost samples were of black color owing to the formation of humic substances, carbon ... cellulose and xylan are present in the form of waste paper and plant residues as major constituents of municipal and yard waste [45] For instance, a strain of Scytalidium thermophilum producing...
... the actual site of proteolytic cleavage, data interpretation and the design of followup analyses are straightforward COFRADIC-based sorting of N-glycosylated peptides Glycosylation of asparagines ... COFRADIC procedure was applied to a trypsin digest of 10 lL of mouse serum depleted for its three most abundant proteins (albumin, IgGs and transferrin), and resulted in the characterizationof ... after being metabolized in the tissue or organism to form reactive products One of the drawbacks of COFRADIC relates to the segmentation of the peptide separation flow during the primary run:...
... unknown but in view of the extensive differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation ⁄ desensitization [20–23] and patterns of expression ... isoform of the human thromboxane A2 receptor is a target for prostacyclin-mediated desensitization J Biol Chem 275, 20412–20423 23 Reid HM & Kinsella BT (2003) The alpha, but not the beta, isoform ... Characterizationof the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms Eur J Biochem 269, 4058–4073 26...
... the sets of primers listed in Table After the PCR products were digested with KpnI and SacI, the fragments were inserted into the KpnI/SacI site of Fig Structures of the 5¢-flanking region of mouse ... transcription start sites for the integrin a3 subunit gene, a modified method of 5¢-RACE using a cap site-labeled cDNA library was employed, recently developed for rapid examination of 5¢-end of genes [33] ... The members of the Ets-family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the expression of numerous genes that are critical for various...
... degree -of- fold, Q, is responsible for high yield stress of biological protein materials through breakage of hydrogen bond of β-sheet structural motif For deeper understanding the role of native ... materials, and that mechanical characterizationof protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterizationof biological molecules ... strain Δε0 for a given position vector r of a protein structure is in the form of u ( r ) = Δε ⋅ r (1) Accordingly, the position vector r* of a protein structure after application of discrete,...
... ion source potential of 70 eV and with an ion source temperature of 200 °C Identification of compounds was done by comparing the mass spectra with the NIST database A novel route for geranial formation ... cleavage of apo-10¢-lycopenal (C27; substrate II) led to a more complex mixture of products, including three compounds (products 1, and 5) identified as dialdehydes on the basis of the fine structure of ... preferences for the three cleavage sites To gain insights into the preference of OsCCD1 for the three cleavage sites, we determined the relative amounts of the C17, C20 and C22 dialdehyde products formed...
... Fernandez-Montalvan et al Biochemical characterizationof USP7 Fig Enzymatic characterizationof USP7 (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC (j), SUMO-1-AMC (d) and Nedd8-AMC ... inhibitor of jB kinase, a regulatory role can be presumed for USP7 phorsphorylation The identification of K869 as the ubiquitination site of USP7 represents additional evidence for the interaction of ... Institutes for Biomedical Research (NIBR) Journal compilation ª 2007 FEBS 4261 ´ ´ A Fernandez-Montalvan et al Biochemical characterizationof USP7 Table Kinetic parameters for the hydrolysis of Ub-AMC...