characterisation of tissue engineering constructs by raman spectroscopy and x ray micro computed tomography µct ioan notingher and julian r jones

DISCRIMINATION OF COLOR COPIER/LASER PRINTER TONERS BY RAMAN SPECTROSCOPY AND SUBSEQUENT CHEMOMETRIC ANALYSIS

Ngày tải lên : 24/08/2014, 11:52

... manufacturers are interchangeable or compatible, (e.g Canon and Kodak, Sharp and Ricoh, Konica and Minolta, and Okidata and Xerox) For example, a Xerox cartridge can be used in an Okidata printer, ... methods often referred to as chemometrics 1.1 Copier Toners and Their Analysis 1.1.1 Background There are two main types of printer cartridges: inkjet and xerographic toner The latter is used ... xerographic printing process Copolymeric resins make up anywhere from 65-80% of the mixture that is used in toner Exact formulations vary from manufacturer to manufacturer and are considered trade...

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DISCRIMINATION OF COLOR COPIER/LASER PRINTER TONERS BY RAMAN SPECTROSCOPY AND SUBSEQUENT CHEMOMETRIC ANALYSIS

Ngày tải lên : 24/08/2014, 12:40

... factor PIAS: Protein inhibitors of activated STAT RANKL: Receptor activator of nuclear factor kappa-B ligand RGD: Arginine-Glycine-Aspartic acid ROS: Reactive oxygen species xii Runx2: Runt-related ... matrix of bone CTSK is primarily expressed by the osteoclast, with some expression occurring in the lung [28] The gene for CTSK is found on chromosome and transcription of CTSK is activated by RANKL ... side of the receptor, which leads to recruitment of STAT3 JAKs phosphorylate a tyrosine residue of the YXXQ motif on the receptor STAT3 can also be activated by the receptors for epidermal growth...

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Báo cáo hóa học: " Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy" pot

Ngày tải lên : 20/06/2014, 02:20

... air The authors also pointed out the possible observation of these modes by micro -Raman spectroscopy They neither (1) performed the micro -Raman experiments; nor (2) calculated/predicted the Raman ... involved in the vibration To understand what types of modes are being excited by the Raman spectroscopy experiment, we turn to a theoretical analysis of the problem There are several routes that can ... spectral resolution of our Raman system is about 1.0 cm-1, we conclude that the relatively broad Raman peak observed here likely resulted from inhomogeneous broadening In order to further test our...

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Raman spectroscopy and field electron emission properties of aligned silicon nanowire arrays

Ngày tải lên : 16/03/2014, 15:19

... TEM carbon support ﬁlm Raman scattering measurements were performed using a Renishaw (RMRe1000) micro -Raman spectrometer at room temperature Raman scattering modes were excited by means of the ... and porous silicon [23] and SiNWs fabricated by thermal evaporation [12] According to the theoretical model proposed by Richter et al [24] and Campbell et al [25], the ﬁrst-order Raman spectrum ... deionized water, blown dry in air and subjected to further analysis Sirion FEG (Philips XL30) scanning electron microscopy (SEM) attached with an energy-dispersive X- ray spectrometer (EDXS, Genesis7000...

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báo cáo hóa học: " An overview of tissue engineering approaches for management of spinal cord injuries" docx

Ngày tải lên : 19/06/2014, 10:20

... retrograde degeneration of corticospinal tract axons and increases axonal regenerative activities in rats Regenerating axons growing from the rostral part of the lesion cross the implant and ... with peripheral nerve fragments lead to partial recovery of paraplegic rats [32] Improved motor recovery and reduced spinal cyst formation of rats was also observed by implantation of macrophages ... after cryopreservation [48] and after establishment of a clonally derived cell line [49] Boosting of regenerative capability of OECs by overexpression of brain-derived neurotrophic factor (BDNF)...

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Tissue Engineering II Basics of Tissue Engineering and Tissue Applications doc

Ngày tải lên : 27/06/2014, 10:20

... membranes · Microenvironment · Microreactors · Reaction engineering · Reacting systems · Transport phenomena · Tissue engineering Introduction 1.1 Overview and Motivation Major thrust areas of research ... engineered tissues The engineering of mechanisms to properly replace the role of neighboring cells, the extracellular matrix, cyto-/chemokine and hormone traf- Controlling Tissue Microenvironments ... Our approach is ﬁrst to understand the requirements of speciﬁc tissue systems and to know how the microenvironment of each meets its particular needs This requires an understanding of the microenvironment...

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PRINCIPLES OF TISSUE ENGINEERING 3RD EDITION - PART 1 pot

Ngày tải lên : 29/06/2014, 09:21

... Biomedical Research Cambridge, MA 02143 FM_P370615.indd xxi xxi Gregory R Dressler Department of Pathology University of Michigan Ann Arbor, MI 48109 George C Engelmayr, Jr Harvard-M.I.T Division of ... 2007, Elsevier, Inc All rights reserved 6/8/2007 4:03:16 PM CHAPTER ONE • THE HISTORY AND SCOPE OF TISSUE ENGINEERING most extreme form of reconstructive surgery, transferring tissue from one individual ... Reichenspurner Department of Cardiovascular Surgery University Medical Center Hamburg-Eppendorf D-20246 Hamburg Germany Ellen Richie MD Anderson Cancer Center University of Texas Smithville, TX 78957...

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PRINCIPLES OF TISSUE ENGINEERING 3RD EDITION - PART 2 (end) doc

Ngày tải lên : 29/06/2014, 09:21

... vitro and prematurely express markers associated with terminal differentiation (Brissette et al., 1996) Furthermore, when Foxn1 is overexpressed, markers associated with earlier stages of differentiation ... ISEMFS • of BM-derived gastrointestinal epithelial cells expressed the proliferative marker Ki-67 and a larger proportion of expressed markers of terminal differentiation (interestingly, transplanted ... between Foxn1 and Six1 Further studies are required to determine whether a regulatory relationship exists between these genes and Foxn1 The expression patterns of Foxn1 and Gcm2 clearly deﬁne...

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New strategies for global optimization of chemical engineering applications by differential evolution

Ngày tải lên : 09/09/2015, 18:55

... Prof S B Chen, Assoc Prof M S Chiu, Prof I A Karimi, Assoc Prof S Lakshminarayanan and other professors in the Chemical and Biomolecular Engineering Department who have contributed directly or ... 111 4.8 NFE and SR of IDE with Four Different Stopping Criteria (SC-1, SC-2, NRmax and Gmax) for Solving VLE-EIV Problems 113 XX 4.9 Performance and Time Required by BARON for VLE-EIV Problems 115 ... NFE and SR of IDE with Four Different Stopping Criteria: SC-1, SC-2, NRmax and Gmax for Solving VLE-LS problems with NP = 50D 107 4.7 The Performance and Time Required by BARON for VLE-LS Problems...

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Automatic microassembly of tissue engineering scaffold

Ngày tải lên : 11/09/2015, 09:17

... dimensions of the microparts were carefully controlled to achieve desired friction between microgripper and microparts and between microparts Factors that affect the qualities of the microparts were ... Microgripper and Part, and between Parts 43 3.5 Properties of Fabricated Microparts 49 3.6 Summary and Discussion 50 Design and Fabrication of Microgripper 53 4.1 Design of ... image of naked gripper (cross) 140 LIST OF FIGURES xiv Figure 7.12: Sharpness of 20 images (backgrounds are part wafer): image of gripper with part (circle); image of naked gripper (cross)...

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Chapter 2 literature review design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 10:20

... control temperature over for micro- architecture, problems with residual solvent Highly porous structures, Phase separation Lack of control over micro- architecture, allows limited range of pore ... by the researchers at the University of Freiburg (Landers and Rolf, 2000) and was termed as ‘bioplotter’ This technique involves a moving extruder head (x- y-z control) and uses compressed air ... results, microstereolithography (µSL) shows high potential in the production of 35 Chapter Two: Literature Review 3D polymeric structures with micrometer resolution Presently, some researchers...

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Chapter 4 results discussions design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 10:23

... row: plan view & bottom row: x- sectional view Extrusion Pressure: Similar to liquefier temperature, increase of extrusion pressure caused the increase of RW and thus decreased the pore size and ... From the rapid prototyping viewpoint, the diblock and the triblock copolymers offered a wide range of temperatures for practical thermal processing The X- ray diffraction pattern of mPEGOH exhibited ... – 4.12 The graphs demonstrate that the liquefier temperature and extrusion pressure affect in the similar manner i.e with the increase of temperature or pressure the RW increases and consequently,...

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Chapter 5 conclusions recommendations design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 10:23

... mm the pore width (in x- or ydirection) and porosity varied from 500 to 1000µm and 50 to 66%, respectively Besides, the process parameters (liquefier temperature, extrusion pressure and deposition ... control the pore shape, size and porosity The 0/90 lay-down pattern produced quadrangular pores, 0/60 pattern resulted in triangular pores, whereas 0/30 pattern formed complex polygonal pores ... scaffold processing The DRBRP was designed to give the user freedom to process a large variety of polymers and/ or scaffolds Virtually, DRBRP system would allow a wide range of thermoplastic material...

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Design and development of tissue engineering scafflods using rapid prototyping technology chapter 3

Ngày tải lên : 14/09/2015, 11:12

... copolymers were characterized by H nuclear magnetic resonance (1H NMR), size exclusion chromatography (SEC), infrared spectroscopy (IR), differential scanning calorimetry (DSC), X- ray diffraction, and ... tetrahydrofuran (THF) and eluted in a series of configurations through a Styragel column refractor (Waters, Milford, MA) at a flow rate of 1mL/min Polystyrene standards (Polysciences, Warrington, ... pore interconnectivity) was characterized by an algorithm similar to mercury intrusion porosimetry (Abell, 1999) In other words, by starting at the periphery of the scaffold and working inwards,...

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Design and development of tissue engineering scafflods using rapid prototyping technology

Ngày tải lên : 14/09/2015, 11:12

... micro- featured environment with natural extracellular matrix comprised of a complex mixture of pores, ridges, fibres and networks Fundamental understanding of interactions between cells and their living ... Chapter One: Introduction 1.1 Background At present, many strategies for tissue engineering (TE) require scaffolds that, by acting as a surrogate extracellular matrix, provide an initial site for ... that requires preparation of filament or any other pre-cursor This work involves design and manufacturing of the necessary hardware namely, liquefier or melting chamber, insulating jacket for the...

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Tài liệu Báo cáo khoa học: Steady-state and time-resolved ﬂuorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Ngày tải lên : 20/02/2014, 23:20

... apo-CRP structure induced by binding of cAMP and DNA by measuring uorescence resonance energy transfer (FRET) and uorescence anisotropy decay Two tryptophan residues, Trp13 and Trp85, were used ... from the equation: QD ẳ QRF SD ARF SRF AD 1ị where SD and SRF are the respective areas under the emission spectra of the donor and a reference compound, and ARF and AD are the respective absorbances ... absence and presence of acceptor and efciency of energy transfer are presented in Table The transfer efciency varied between apoprotein and after binding cAMP at micromolar or millimolar concentrations...

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Best Practices in Social Media / Summary of Findings from the Second Comprehensive Study of Social Media Use by Schools, Colleges and Universities docx

Ngày tải lên : 08/03/2014, 19:20

... your current position? Executive director, director or manager Associate, assistant or deputy director/manager Specialist, coordinator, or administrative assistant Associate or assistant vice president ... Survey • Summary Topline Findings • April 13, 2011 Overview of findings Background Slover Linett Strategies Inc and mStoner partnered with CASE for the second year in a row in order to research ... that person (selected above) report to? Executive director, director or manager Vice president, vice chancellor or assistant head of school Associate, assistant or deputy director/manager Associate...

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Báo cáo khoa học: Signalling and regulation of collagen I synthesis by ET-1 and TGF-b1 doc

Ngày tải lên : 23/03/2014, 11:20

... purified RNA were reverse-transcribed by reverse transcriptase core kit (Eurogentec, Cologne, Germany) and subjected to PCR in the presence of specific primer pairs (ETA-receptor: forward primer: 5¢-TGCCCTCAGTGAACATCTTAAGC-3¢, ... myocardial fibroblasts a shift towards the ETA-receptor profoundly interferes with cellular Ca2+ regulation [57] Moreover, it is described that the ETA-receptor regulates proliferation by proand ... according to the manufacturer’s instructions RNA loading and transfer was evaluated by re-probing the membranes with an 18S ribosomal RNA cDNA probe Real Time PCR (RT-PCR) Two lg of purified RNA...

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Báo cáo khoa học: Speciﬁc Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf

Ngày tải lên : 30/03/2014, 02:20

... USA) Resonance Raman and near-infrared FT -Raman spectroscopy Room-temperature resonance Raman spectra (spectral resolution of 1.4 cm)1) were recorded with a liquid nitrogencooled CCD detector (SPEC-10-400B ... Near-infrared FT -Raman spectroscopy 1170 (R4 ) Resonance Raman spectroscopy all-trans spirilloxanthin in LH1 The Raman bands from 15-cis spirilloxanthin in the RC normally seen at 1528, 1239 and ... b-polypeptides of LH2 from Rps acidophila (PDB record 1nkz) and Rs molischianum (PDB record 1lgh) as well as those of LH1 from Rs rubrum (PDB records 1xrd and 1wrg) were used as the templates MODELLER is...

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báo cáo hóa học:" Detection of postoperative granulation tissue with an ICG-enhanced integrated OI-/X-ray System" docx

Ngày tải lên : 18/06/2014, 15:20

... absence of visibly increased fluorescence in the region of surgery compared to normal contralateral muscle An increased fluorescence of the left paravertebral soft tissues was interpreted as presence ... standard deviation (SD) and the relative fluorescence signal enhancement (ΔSI%) of the paravertebral soft tissue in the area of previous surgery of the experimental group compared to the controls ... manually repositioned by the operator in order to match the anatomical area of surgery ΔSI was calculated by subtraction of SI of the postoperative granulation tissue from the SI of the normal muscle:...

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