cellularity communities in stationary phase cultures stem cell niches and aging

Tài liệu Review of Chronic Graft- Versus-Host Disease in Children After Allogeneic Stem Cell Transplantation: Nursing Perspective pptx

Tài liệu Review of Chronic Graft- Versus-Host Disease in Children After Allogeneic Stem Cell Transplantation: Nursing Perspective pptx

Ngày tải lên : 12/02/2014, 19:20
... mends a system for scoring chronic GVHD manifestations in organs and sites, including the skin, mouth, eyes, gastro­ intestinal tract, liver, lungs, joints and fascia, genital tract, and performance, ... (Th2) cells can initiate a response against autoantigens leading to B -cell hyperre­ activity and production of autoantibodies, causing targetorgan damage, including skin, mucosa, eyes, joints, and ... GVHD is initiated, T-cells produce additional proinflammatory cytokines, including tumor necrosis fac­ tor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-2, which in turn attract more T-cells...
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Báo cáo sinh học: " Risk factors in the development of stem cell therapy" pdf

Báo cáo sinh học: " Risk factors in the development of stem cell therapy" pdf

Ngày tải lên : 18/06/2014, 19:20
... stem cells that originate from the inner cell mass of a blastocyst stage embryo The embryonal carcinoma cells replicate and grow in cell culture conditions In 1981, embryonic stem cells (ES cells) ... between somatic stem cells and cancer stem cells (self renewal, asymmetric division and relative slow proliferation) a direct link between somatic stem cells and cancer stem cells remains to be shown ... by cell sorting to minimise the number of pluri/ multipotent stem cells in the cell preparation [86], or to separate tumourigenic stem cells from non-tumourigenic stem cells (by e.g cell sorting...
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Báo cáo y học: "Marked increase of procalcitonin after the administration of anti-thymocyte globulin in patients before hematopoietic stem cell transplantation does not indicate sepsis: a prospective study" pptx

Báo cáo y học: "Marked increase of procalcitonin after the administration of anti-thymocyte globulin in patients before hematopoietic stem cell transplantation does not indicate sepsis: a prospective study" pptx

Ngày tải lên : 13/08/2014, 16:20
... antibody administration [8] Pihusch and colleagues [9] prospectively studied PCT, CRP, and interleukin-6 (IL-6) in 350 stem cell recipients Conditioning with ATG increased all monitored markers In neutropenic ... (Figure 2) Microbiological cultures of the pharyngeal, nose, and rectal swabs and urine samples yielded clinically insignificant results Blood cultures obtained during conditioning did not grow any ... (GGT), and bilirubin to assess liver function and of urea, creatinine, and glomerular filtration rate (GFR) to assess renal function Pharyngeal, nose, and rectal swabs and urine samples were obtained...
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Báo cáo y học: "Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress" ppt

Báo cáo y học: "Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress" ppt

Ngày tải lên : 14/08/2014, 16:21
... µM menadione exposed Time course at minute intervals in cells from stationary phase cultures Time course at minute intervals in cells from stationary phase cultures exposed to 50 µM menadione ... puzzling refereed research Figure 1to 50 µM minute intervals in cells from stationary phase cultures exposed Time course at 30 menadione Time course at 30 minute intervals in cells from stationary ... 10 minute time point after re-feeding cells in stationary phase cultures, there were significant increases in over 1,000 transcripts [14,36] The major overlaps in RNAs that increased during both...
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Báo cáo y học: "Correction: Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress" pot

Báo cáo y học: "Correction: Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiae produces a massive increase in transcript abundance in response to stress" pot

Ngày tải lên : 14/08/2014, 17:22
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1

Ngày tải lên : 09/09/2015, 17:54
... to induce pluripotency in adult somatic cells (Takahashi and Yamanaka, 2006), further demonstrating the pivotal role of transcription factors in the maintaining and inducing the pluripotent cell ... brain, forming the central nervous system Following neural tube closure, patterning is important for determining regional cell fate, and this is mediated by signaling between adjacent cells by cellsurface ... morphogenetic protein (BMP) antagonists such as chordin, noggin and cereberus BMP4 is pivotal in neural induction, as it inhibits cells from forming neural tissue (Finley et al., 1999) Therefore, inhibition...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 2

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 2

Ngày tải lên : 09/09/2015, 17:54
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3

Ngày tải lên : 09/09/2015, 17:54
... target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent ... governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation   104  Chapter VII – Long Non-coding RNAs are Indispensable in ... lncRNAs using anti-SUZ12, anti-SOX2 and anti-OCT4, respectively lncRNA_ES1 and lncRNA_ES2 were enriched in the SUZ12 RIP and SOX2 RIP, indicating interaction * and ** indicate p-values of < 0.05 and...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4

Ngày tải lên : 09/09/2015, 17:54
... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... silencing factor, is a transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the ... expressed in the midbrain and prospective dopamine neurons Rmst transcript was detected by in situ hybridization in the mouse embryonic brain (A, B) Rmst as detected in both thr roof plate (RP) and...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5

Ngày tải lên : 09/09/2015, 17:55
... is a RNAbinding protein and probably binds directly to RMST SOX2 and hnRNPA2B1 may interact via protein-protein interactions The transcription factor SOX2 may then bind to target chromatin regions ... protein-coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS One 6, e28318 Chen, M., Zhang, J., and Manley, J.L (2010) Turning on a fuel switch of cancer: hnRNP proteins ... elucidated in this thesis Data indicated that RMST is abundant in neurons, and it interacts with the SOX2 via the RNA-binding protein hnRNPA2B1 Expression analyzes following perturbations of cellular...
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Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation

Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation

Ngày tải lên : 11/09/2015, 09:02
... signalling pathways maintaining ES cell pluripotency Embryonic stem cells are maintained by a network of extrinsic and intrinsic signals that collectively regulate the properties of pluripotency and ... pathways involving Zic3 The Zic and Gli family proteins physically interact through their zinc finger domains to regulate neural and skeletal patterning117,118, and the Zic proteins are known to bind ... Zic3 and Sox2 co-occupy physical binding sites in mouse ES cells 4.3 Discussion 4.3.1 Zic3 and Sox2 regulate a common set of pathways in ES cells 4.3.2 Zic3 and Sox2 are interacting partners in...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2

Ngày tải lên : 11/09/2015, 16:06
... L-glutamine, 10mg/mL streptomycin and 10U/mL penicillin The cells were cultured in an incubator at 37°C, 5% CO2 in a humidified environment 2.1.2.1.2 Over-expression of SPHK1 and SPHK2 in CHO Cells ... inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF and SOV) were freshly added each time before using Substrate preparation: 1mM D-erythro-sphingosine was prepared by mixing 20μl sphingosine (50mM) ... lymphoma cell line The cells were cultured in RPMI 1640 supplemented with 10% FBS (GIBCO, Invitrogen Singapore), 2mM glutamine, 10U/ml penicillin and 10mg/ml streptomycin at 37C, 5% carbon dioxide in...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3

Ngày tải lên : 11/09/2015, 16:06
... streptomycin and 10U/ml penicillin Cells were cultured in an incubator at 37°C, 5% CO2 in a water-saturated environment 3.1.1.3 Stem Cells Seeding Density Unless otherwise stated, stem cell seeding ... Penicillin and Streptomycin DMEM+10% FBS +1% Penicillin and Streptomycin Supplements N/A 0.5mM isobutyl-methylxanthine, 1μM dexamethasone, 10μM insulin, 200μM indomethacin, 1% Penicillin and Streptomycin ... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4

Ngày tải lên : 11/09/2015, 16:06
... would be convincing that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally ... cultured in 6-well plates were washed twice with 1x PBS and starved in serum-free DMEM for half an hour 1μM S1P was then added into the wells and incubated at 37°C for 5min, 10min, 30min, and 60min ... S1P in human BM- and AD- MSCs proliferation proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells in...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5

Ngày tải lên : 11/09/2015, 16:06
... the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, ... expanded to study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem ... for SPHK inhibitors in adult -stem cell differentiation In the final part of my research project (Chapter 4), the bioactive lipid S1P was studied for its role in promoting human BM- and AD- MSCs...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6

Ngày tải lên : 11/09/2015, 16:06
... “Charaterization of N,N-dimethyl-Derythro-sphingosine-induced apoptosis and signaling in U937 cells: Independece of sphingosine kinase inhibition.” Prostaglandins & other lipid Mediators 86: 18-25 Kim, ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...
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Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation

Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation

Ngày tải lên : 11/09/2015, 16:06
... Therefore, mediating stem cells differentiation, in particular, promoting stem cells differentiation, in a controlled way, would be highly desirable in stem cells research and in their future clinical ... particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult stem cell proliferation and differentiation ... S1P in stem cells research, derived from the current understanding on human stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS...
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The role of microRNAs in embryonic stem cell development and differentiation

The role of microRNAs in embryonic stem cell development and differentiation

Ngày tải lên : 12/09/2015, 08:16
... receptor Multiple cloning site Methyl-CpG-binding proteins Mouse embryonic stem cell Midbrain-hindbrain isthmus/boundary MicroRNA MicroRNA MicroRNA-containing RNA-induced silencing complexes MicroRNA ... Seng, Huibin, Yin Loon, Minh, Sandy, Phil and all other past and present members of Bing’s lab, thank you for your friendship, and for making the lab such a stimulating and fun place to work in I ... synthase kinase-3 High content screening Histone deacetylases Human embryonic stem cell Inner cell mass Inhibitor of differentiation In situ hybridization Janus-associated tyrosine kinase Leukemia inhibitory...
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Clinical significance of cancer stem cell markers and other related proteins in colorectal carcinomas biological and methodological considerations

Clinical significance of cancer stem cell markers and other related proteins in colorectal carcinomas biological and methodological considerations

Ngày tải lên : 03/10/2015, 20:57
... also maintain their inherent stem cell capacity to self-renew and differentiate In this thesis, the molecular and clinical significance of cancer stemcell markers and its related proteins in colorectal ... considered as a stem cell disease These studies suggest that many signalling pathways involved in the maintenance of normal stem cells are mutated in human cancers – these include WNT, beta-catenin, TGF-beta ... transformation involving stem cells (Figure 3) The first step is that the tumour is initiated by a single mutation that disrupts the regulated asymmetric division in stem cells The mutated “cancer” stem cells...
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