cells hscs and kupffer cells proliferate due to cde diet and likewise rapidly growing liver related cell lines express m2 pk
Báo cáo y học: "Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet" ppsx
... former CDE study using C57Bl/6 mice [5] and confirmed our results (data not shown) Oval cells, HSCs and Kupffer cells proliferate due to CDE diet and likewise rapidly growing liver related cell lines ... stellate cells (HSCs) [9] and Kupffer cells [10] of rat liver A switch from M1- to M2- type was demonstrated in rapidly growing cells [11], and M2- type was found to be expressed in oval cells [12,13] ... findings in rats that endothelial cells, biliary cells, Kupffer cells [7,10] and HSCs [9] express M2- Pk Furthermore, also infiltrating immune cells may contribute to M2- Pk expression demonstrated by...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... susceptibility of these cell lines to DNR-induced apoptosis (40.15% in KG1a cells and 86.23% in Kasumi-1 cells) , compared to DNR only (3.17% in KG1a cells, 5.94% in Kasumi-1 cells) These results ... KG1a and Kasumi- 1cells were insensitive to DNR KG1a, Kasumi-1 and U937 AML cells were stained with PE-conjugated CD34 antibody and subjected to flow cytometry to determine the purity of CD34+ cells ... progenitors to current chemotherapies CD34 + AML cells are 10-15-fold more resistant to DNR than CD34- AML cells [8] CD34+ KG1a and TF-1 AML cell lines are 30-40 fold more resistant to mitoxantrone...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
... correlations were used to assess comethylation between probes and to compare methylation and gene expression We used 10,000 permutations of the gene expression to methylation assignments to assess the ... 12:R10 http://genomebiology.com/2011/12/1/R10 cells using phytohemagluttinin We obtained the transformed cell lines from the Coriell Cell Repositories Methylation data were obtained using the ... methylation is negatively correlated with gene expression (a) Methylation levels are low in the top quartile of highly expressed genes (left), and high in the bottom quartile of lowly expressed genes (right),...
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báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
... Figure Cells growth curve and MTT analysis for MDA-MB-231 and MCF-7 cells A: cells growth curve analysis for MDA-MB-231 and MCF-7 cells B: MTT analysis for MDA-MB-231 and MCF-7 cell compared to blank ... continued to observe for days, drew growth curve The 96 filter were seed with × 103 cells/ filter, and cells were cultured for 24, 48, 72 and 96 hours, respectively, then added 20 ul MTT to cells and ... MDA-MB-231 cells lane 4: blank control group in MCF-7 cells lane 5: PG group (empty vector) in MCF-7 cells lane 6:PGM2 group in MCF-7 cells B: Column diagram analysis for protein expression of...
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Báo cáo y học: "Biochemical and morphological changes in endothelial cells in response to hypoxic interstitial edema" pdf
... volume and surface of endothelial and epithelial cells in the air-blood barrier Histograms of frequency distribution of cytoplasm volume density in endothelial (A) and epithelial (C) compartments and ... HYPOXIA h HYPOXIA h CARDIOG EDEMA Cell volume Normality test Skewness Mean ± SE Median Cell surface Cell volume Cell surface Cell volume Cell surface Cell volume Cell surface F F F P P P F F 1.53 ... response to hypoxia (open circles, pooled data from and h), there is a definite trend for the data to scatter towards high Sv values and, correspondingly, very low cell volume in endothelial cells...
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Báo cáo y học: "Thymic plasmacytoid dendritic cells are susceptible to productive HIV-1 infection and efficiently transfer R5 HIV-1 to thymocytes in vitro" pdf
... infected DC to CD4+ T cells [29] Tissue DC isolated from tonsils and skin (Langerhans cells) can transfer both R5 and X4 HIV-1 to CD4+ T cells [13,30]; however, there have not been any studies to determine ... transcription factors in thymic and CD34+ progenitor-derived plasmacytoid and myeloid dendritic cells: evidence for distinct expression profiles Exp Hematol 2004, 32:104-112 20 Keir ME, Stoddart CA, ... fetal liver and thymic tissues (SCIDhu-thy-liv) infected with R5 HIV-1BaL At day post infection, p24+ cells were visible in both the cortex and medulla (Figure 5C), and CD123+ cells were found to...
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Báo cáo y học: " Dendritic cell-mediated HIV-1 transmission to T cells of LAD-1 patients is impaired due to the defect in LFA-1" docx
... virions and expression of LFA-1 on target cells [36-40] To critically test this hypothesis, we performed transmission experiments with HIV-1 produced both in C33A cells and in PM1 T cells C33A cells ... added an inhibitor of the viral protease (saquinavir, [23,24]) To distinguish virus transmitted to T cells from HIV in DC, we co-stained with CD3 and DC-SIGN DC and T cells tend to cluster, which ... 100 10 1 days LAD-1 and LAD-1/variant T cells form smaller clusters with DC DC attract T cells and form large clusters in vivo and in vitro, a process that is dependent on cell- cell adhesion [26,27]...
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Dendritic cells respond differently to live and killed bacteria molecular mechanisms of pathogen recognition by dendritic cells and implications for vaccine development
... antibodies (for B cells) and the generation of armed effector T cells such as cytotoxic T cells and helper T cells (Th) (for T cells) Some of these B and T cells will eventually become memory cells The ... Toll-like receptors (TLRs) expressed on antigen-presenting cells (APC), such as dendritic cells, upregulates cell- surface expression of co-stimulatory (CD80 and CD86) molecules and major histocompatibility ... priming of both B and T cells, an understanding of how E.coli interacts with APCs such as Dendritic cells which secretes cytokines that primes both B and T cells, could potentially leads to the development...
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Analysis and compensation of log_domain filter deviations due to transistor nonidealities
... Introduction LOG operator EXP opemtor Figure 1-4: Signal companding by LOG and E X operators represent the log-domain inputs and output, respectively Due to the LOG-linear-EXP format of the cell, an isolated ... log-domain cells, (b) log-domain 41 integrator, and (c) log-domain biquad Effects of fmite beta on filter Q factor 42 (a) Compensation of the nonideal effects due to finite beta, and ... as an example, pris marily due to its simplicity and sufficiency to reveal the undedying principles Here, we follow a bottom-up approach To begin with, the log-domain cells wiU be analyzed under...
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Children’s Health Deficits due to Diarrhoea: Effects of Water Supply and Sanitation Systems in Slums with Different Water Logging Conditions
... Short-term and Heavy rainy, SDG: Short-term and Drainage, LSD: Long-term and Stagnant, LHT: Long-term and Heavy rainy, LMK: Long-term and Monsoon flood, PDL: Persistent and Drainage, PHS: Persistent and ... correlation between DALYs lost due to diarrhoea and some factors relevant to hygiene practices, including using overhead hanging latrines, not washing hands before eating and after defecation, as well ... model life tables and, due to disability from age a to age (a + L), consists of the following equation derived by Murray (1994) and Homedes (2000): - 280 - Journal of Water and Environment Technology,...
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Energy savings due to daylight and artificial lighting integration in office buildings in hot climate
... Saudi Arabia Square 10 3.6 m 0.53 W /m2. 0C 0.61 W /m2. 0C 0.94 W /m2. 0C 0.51 W /m2. 0C 0.5 for external walls and the roof 15 m2/ person [14] 22 W /m2 [15] & [16] 15 W /m2 [17] 0.5 ACH (building with average ... consuming a large proportion of the total energy to maintain lighting requirements and visual comfort As much as 20% of the total energy consumed by an office building goes to lighting [10] Therefore, ... practiced designs and operational characteristics as revealed from survey questionnaire on selected local design offices and interviews of building operators as well as relevant standards and logic judgment...
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The NCI Strategic Plan for Leading the Nation To Eliminate the Suffering and Death Due to Cancer pdf
... tumor stem cells and characterize the interactions between these cells and stromal cells > Continue investigations to describe the role of inflammatory and immune cells in tumor initiation and progression ... elucidates the dynamic and spatial interactions among molecules in a cell, among cells, between cells and their microenvironment, and between the organism and its macroenvironment, and considers differences ... cancer cells from the primary tumor become less adhesive to the cells around them, break loose, and enter either the circulatory or lymphatic system Cells in the lymphatic system may come to rest...
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Health Education to Villages: An integrated approach to reduce childhood mortality and morbidity due to diarrhoea and dehydration; Maharashtra, India 2005 – 2010 pptx
... toilets, increased advocacy and education will be necessary to convince people to use the toilets, to create separate male and female toilets in schools, and to promote proper practices of hand ... recognizing symptoms of dehydration and properly managing diarrhoea, and on promoting these practices to mothers It is usually diarrhoea which first brings babies into contact with doctors and the health ... bottle, with a label about how to recognize signs and degrees of dehydration, how to prepare home fluids for rehydration, and how to mix and prepare home-made and packaged ORS Additionally, they...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... amongst tumor cell lines, including inflammatory cytokines To reduce background differences in gene expression related to tissue-specific expression patterns, a group of human HNSCC cell lines consisting ... that will help elucidate the role of these cells in the ontogeny, spread, and treatment of cancer Page of 20 Methods Cell Lines and Cell Culture Tumor cell lines were obtained from the American Type ... comparisons to medium only CD33+ controls C, Heatmap showing expression of immune modulatory cytokines by HNSCC cell lines in relation to their ability to induce CD33+ MDSC MDSC-inducing cell lines...
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Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio" potx
... both costimulatory and survival signals to CD4+ T cells and induces Th17 cytokine expression [25,26] However, it is still not clear if C5a can impact human T cells and if Th17 cells are associated ... staining buffer Cells were then washed and subsequently sorted on a FACS Aria (BD Biosciences) BD FACSDiva software was used to sort the cells Cell culture and flow cytometry PBMC cells were cultured ... (Minneapolis, MN) and were performed based on kit protocols Apoptosis Assay Cell sorting To sort CD4+ T cells and monocytes, × 10 PBMCs were stained with allophycocyanin-labeled CD3 (clone Apoptotic cells...
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Báo cáo sinh học: " Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus" ppt
... compared the cytopathogenicity and virus yields of the viruses in normal CHSE-214 cells and in cells constitutively expressing ASMx1 The CHSE-214 cells constitutively expressing ASMx1 [15] were a ... propagated and titrated in CHSE-214 cells and/ or TO cells as previously described [6,8] To demonstrate the effects of ASMx1 on the replication of the two ISAV strains, we compared the cytopathogenicity ... CHSE-214 cells CHSE-214 cells1 CPE development2 CPE severity3 Virus titer4 Normal ASMx1 overexpression 5–14 days 6-* days Complete (5+) Incomplete (3+) 5.50 4.50 1CHSE-214 cells: Normal CHSE-214 cells...
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báo cáo hóa học:" Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus" docx
... compared the cytopathogenicity and virus yields of the viruses in normal CHSE-214 cells and in cells constitutively expressing ASMx1 The CHSE-214 cells constitutively expressing ASMx1 [15] were a ... propagated and titrated in CHSE-214 cells and/ or TO cells as previously described [6,8] To demonstrate the effects of ASMx1 on the replication of the two ISAV strains, we compared the cytopathogenicity ... CHSE-214 cells CHSE-214 cells1 CPE development2 CPE severity3 Virus titer4 Normal ASMx1 overexpression 5–14 days 6-* days Complete (5+) Incomplete (3+) 5.50 4.50 1CHSE-214 cells: Normal CHSE-214 cells...
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