cell s dna genome rna is synthesized in the nucleus and exported to the cytoplasm
báo cáo khoa học: "The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress" ppt
... t-test) (H) and (I) Histochemical analysis of GUS expression in pVvMYB60:GUS leaves in response to ABA (H) GUS staining of stomata in a control leaf (I), GUS staining of stomata following hours ... the same conditions, the lines expressing the VvMYB60 gene displayed a rate of water loss indistinguishable from the one observed in the wildtype, thus demonstrating complete rescue of the stomatal ... function in guard cells Expression features of VvMYB60 in grape organs It has been previously shown that the Arabidopsis AtMYB60 gene is expressed in seedlings, rosette leaves, stems and flowers and...
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Regulation of DNA (cytosine 5) methyltransferase 1 in the cell cycle and its role(s) in doxorubicin mediated micronuclei formation
... DNMT1 is essential for maintaining CpG island hypermethylation in colorectal cancer cells, casting doubts to the presumed role of DNMT1 in previous studies These conflicting findings were resolved ... of Topoisomerase IIα The SET and RING fingerassociated (SRA) domain of UHRF1 shows preferential binding to hemimethylated CG sites and is responsible for the binding and recruitment of HDAC1 to ... 2001) DNMTs bear ten characteristic sequence motifs, Motifs I to X Most of these motifs are discernable in the DNA cytosine-methyltransferases from bacteria, fungi and plants to mammals (Bestor, 2000)...
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Báo cáo Y học: The plant S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes pot
... amino acids between Arabidopsis and rice proteins) A multiple alignment including the proteins from Arabidopsis and rice and the MgPIXMT sequences from Synechocystis and R capsulatus (P26236) shows ... Expression of truncated portions of the protein, [(a) D80-protein missing the ®rst 79 amino acids and similar in size to the prokaryotic MgPIXMT; and (b) D161-protein missing the ®rst 160 amino ... chlorophyll synthesis, the enzyme must be connected to other enzymes of the chlorophyll synthesis pathway Several enzymes were reported to be present in or to associate with the envelope: the protoporphyrinogen...
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Processing Fluency and Aesthetic Pleasure: Is Beauty in the Perceiver''''s Processing Experience? pptx
... versus exaggerated forms, and why artists and scientists have often considered truth and beauty as two sides of the same coin Processing Fluency The Concept of Processing Fluency The processing ... Perceiver 's History With the Stimulus Psychological research on factors underlying beauty has also investigated the history of a perceiver 's experience with the stimulus This research points to the ... interpret the positive affect elicited by processing fluency as their response to the target, resulting in more positive evaluations This assumption is consistent with the feelings-as-information...
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Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... in the L-MAT T -cell apoptosis induced by TCDD Synthetic peptides corresponding to the pseudosubstrate domains of PKC have been used as specific inhibitors of PKC in in vitro assays N-myristoylation ... cell apoptosis (Fig 1C) at doses suggestive of inhibition of PKCd and PKCh These results indicate that nPKC (PKCd and ⁄ or PKCh) is involved in the TCDD-induced apoptosis of L-MAT cells The incubation ... apoptosis We used several PKC-selective inhibitors to determine whether PKC is functionally involved in the L-MAT cell apoptosis induced by TCDD and attempted to identify the specific PKC isoform (s) ...
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Báo cáo sinh học: "Protein kinase CK2a is overexpressed in colorectal cancer and modulates cell proliferation and invasion via regulating EMT-related genes" docx
... of staining The immunohistochemically stained tissue sections were scored separately by two pathologists who were blinded to the clinical parameters For assessment of CK2a, the entire tissue section ... also assessed CK2a expression in normal-CRC tissue pairs by western blot Similar to the result in our immunohistochemistry assay, CK2a expression was significantly higher in colorectal tumor tissues ... mucosa tissues N: normal colorectal mucosa tissue; T: tumor tissue (B) Quantitative analysis of CK2a protein expression in eight pairs of CRC tissues and adjacent normal colorectal mucosa tissues...
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Báo cáo hóa học: " Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a " pptx
... cleavage in the normal cells Thus, HIV-1 infection induces apoptosis of NBS1-deficient cells in an integrasedependent manner This finding is consistent with the failure of PIR rather than a defect in ... relative to the control cells (Figure 6A) Thus, NBS1 is involved in the joining of viral to host DNA Retroviral infection triggers apoptosis of NBS1-deficient cells in an integrase-dependent manner The ... anti-HIV-1 therapy, since development of resistance against inhibitors of these proteins is unlikely [6,8,9,27,42] NBS1 and its interactive partners, being such co-factors, are thus potential targets...
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Báo cáo y học: "Relaxin''''s induction of metalloproteinases is associated with the loss of collagen and glycosaminoglycans in synovial joint" ppsx
... fibrocartilaginous tissues from different sites, including the pubic symphysis and synovial joints These findings also suggest that in fibrocartilaginous tissues, including the TMJ disc and possibly the pubic ... hormones MS retrieved tissues from animals and assisted in several MMP assays SK conceived the study, participated in its design and coordination, supervised the statistical analysis, and wrote the ... increases GAG synthesis [15,16] MMPs contribute substantially to tissue degeneration in inflammatory joint diseases, including rheumatoid arthritis and osteoarthritis [33-35] Our findings show...
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Tài liệu A Women’s Health Intervention for Gynecological Problems in the Deployed Environment ppt
... to avoid vaginitis, UTIs, and menstrual symptoms during deployment This study has potential of significantly contributing to improving female Soldiers’ readiness and filling a gap in military ... urination) Vaginal symptoms: Itching, burning, pain, & discharge Menstrual complaints: Pain, heavy bleeding, missed or irregular periods, PMS Women in Bureau of Medicine Operational Obstetrics ... feminine • hygiene and menstrual self-care practices lead to a decrease in genitourinary complaints during deployment? Specific Aims To increase knowledge level about feminine hygiene in the...
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Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx
... be obtained We therefore introduced the gene into pET11a, and were then able to successfully express it and then isolate it using the steps summarized in Table E coli strain BL21 CodonPlus RIL ... activity bands with T litoralis (data not shown) These results suggest that P horikoshii and T peptonophilus each produce two distinguishable forms of dyel-proDH Because its genome has been sequenced ... gels and inserted between the SphI and KpnI sites of plasmid pUC19 and the BamHI and SphI sites of pUC18, respectively The E coli strain JM 109 cells were transformed with these recombinant plasmids...
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Tài liệu Báo cáo khoa học: "THERE STILL IS GOLD IN THE DATABASE MINE" potx
... linguistic forms and speech acts than when interfacing to a simple db system areas has been going on for some time and shows no sign of running dry One criticism of the simple db interfaces is ... interest in database interfaces and in which considerable research is needed Large, shiny nuggets of theory are waiting to be discovered by enterprising computational linguists! I Speech input Interest ... expressions are almost entirely absent from simple database query, but this need not be the case The point of the spectrum is that there is a continuum from "database" to "knowledge base", and...
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Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx
... unclear and is dependent on the isoform and the tissue examined; however, it appears to be mainly nuclear in cell lines and brain where, nevertheless, there is additional cytoplasmic expression ... In this study, an XRbm9 isoform expressed at steady state in the oocyte cytoplasm was identified The amino-terminal-most sequence of XRbm9 is particular, as it is extended in comparison with the ... hRbm9 was inserted into the EcoRI-SmaI sites of the pBSKS+ vector to generate pKS hRbm9DN The EcoRVSmaI fragment from this plasmid was inserted in frame with Gal4AD into the SmaI site of the pADGal4...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... tissues These results suggest that transcription of OsPol k reflects the level of cell proliferation and is perhaps related to meiosis OsPol k was actively transcribed in rice cells in suspension ... OsPCNA was also incubated for 10 at 37 °C In this case, the product size was not increased OsPCNA was expressed and purified as described previously [17] Terminal transferase assay A single-stranded ... DNA synthesis Each of the four dNTPs was assayed individually as a substrate for incorporation opposite the four possible template bases, in the presence of Mn2+ ions OsPol k preferentially inserted...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
... brain mitochondria in the presence of these electron donors This is of relevance in brain, because in this tissue glucose is metabolized providing the NADH-yielding substrate pyruvate Under these ... contribute to the induction of oxidative stress during ischemia ⁄ reperfusion in brain There is a growing body of information concerning the mechanism of ROS generation by the respiratory chain in mitochondria ... points towards the involvement of oxidative stress [5,6] Further investigations using animal models of stroke made it clear that mitochondria are injured during cerebral ischemia and postischemic...
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Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc
... on the cell membrane No increase in the cell surface NCT level in cells overexpressing Synoviolin was observed (Fig 5D) Discussion In this study, we showed that Synoviolin is involved in the intracellular ... receptor-like receptor [20] In this study, we addressed whether Synoviolin is involved in the degradation of PS cofactors using synoviolin-null cells, as PS cofactors undergo the ubiquitin ⁄ ... We also determined the levels of c-secretase-unrelated ER protein (calnexin) and cytoskeleton protein (tubulin) in these cells as the internal control proteins The calnexin and tubulin levels were...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
... amounts in both tissues [20] Interestingly, Ankrd2 was previously suggested to be cleaved by calpain [29] but CARP, which was shown to be the first MARP whose expression increases in response to ... (Gibco) cDNA were synthesized from lg of total RNA using the SuperScript first strand synthesis system for RT-PCR kit (Invitrogen) and random oligonucleotides Expression of calpain 3, CARP, NF-jB and ... specific signals trigger its dissociation from a muscle specific inhibitor [46] We therefore tested our hypothesis using ectopic gene expression in non muscular cells, the only system leading to uncontrolled...
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Hướng dẫn khắc phục lỗi “username is not in the sudoers file...” trong Ubuntu doc
... /etc/sudoers.d/ instead of # directly modifying this file # # See the man page for details on how to write a sudoers file # Defaults env_reset # Host alias specification # User alias specification ... /etc/sudoers.backup sudo nano /etc/sudoers - Và nhập đoạn mã vào file hiển thị: # # This file MUST be edited with the 'visudo' command as root # # Please consider adding local content in /etc/sudoers.d/ ... theo, chọn Check all file systems - Nhấn Enter hệ thống hiển thị thông báo: Finished, press Enter - Tiếp theo, chọn Drop to root shell prompt: - Khi hình Command Prompt hiển thị, gõ lệnh: usermod...
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Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt
... fluorescence intensity was used for analysis Statistical analysis Values were expressed as the mean ± SE The statistical analysis was performed with Student s t-test For multiple treatment groups, ... expression An expression vector coding a double-stranded RNA (dsRNA) targeting nucleotides 470– 494 of the rat MEF2B mRNA sequence was introduced into A7r5 cells Following single cell cloning of the ... series of deletion mutants of the 5130 Binding of MEF2 to the consensus sequence in the NOX1 promoter To verify whether the transcription factor MEF2 actually binds to the consensus sequence in the...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose