... the cell, working with other factors to halt the cellcycle and mediate DNA repair. Interestingly, a much higher proportion of NFT were labeled in AD cases than in control cases. These findings ... neuronal cellcycle are un-clear. In this regard, given that a dysregulated cellcycle is a characteristic of cancer, we speculated that altera-tions in oncogenic proteins may play a role in neurodegeneration. ... in all cases of Alzheimer disease. These findings not only further define the cellcycle reentry phenotype in Alzheimer disease but also indicate that the neurofibrillary tangles which define...
... gene transcription [3].Interest in control mechanisms of cyclin B1 and cyclinB2 cell cycle- dependent transcription began early[3,30–33]. When investigating the regulation of humancyclin B1 transcription, ... alterations during the cell cycle, making it unlikely that protein binding to theCDE ⁄ CHR is affected by change in DNA methylationduring different phases of cell division [34].Another cellcycle gene, ... Promoterbinding factors regulating cyclin B transcriptionin thesea urchin embryo. DNA Cell Biol 14, 869–881.34 Tscho¨p K & Engeland K (2007) Cell cycle- dependent transcription of cyclin B2...
... aprotinin, leupeptin and pepstatin, twice in kinasebuffer, and used for in vitro kinase assays. In vitro kinase assaysRecombinant human protein kinase CK2 holoenzyme(0.5 U, corresponding to ... 2A,C; in C, only a faint signal wasdetected).The above results indicated that the C-terminus ofRio1p might play a role in the interaction with Cka2p in yeast cells. To determine domains of ... G1phase [11].Keywordsatypical protein kinase; casein kinase 2substrate; cell- cycle phase-dependentdegradation; protein–protein interactions;Rio1 protein kinase21CorrespondenceM. AngermayrE-mail:...
... of the geminin proteindiffers between cell line s, r esulting in gross differences in relative amounts of Cdt1 and its i nhibitor in different cell lines. I t would b e interesting to investigate ... during the cell cycle in human cells, accumulating only du ring the G1 ph ase,when licensing is legitimate [13]. When cells exit the cell cycle, licensing in lost, a nd is est ablished again ... full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti-Cdt1 and anti-geminin Ig. See Materials...
... proteins act as mediators of various cellcycle regulators, in- cluding Cdk activity. The CKS (Cdc28 kinase subunit) protein family isthe mammalian homolog of the yeast Cdk binding proteins Cks1 ... Chaperone protein involved in protein folding, Dix et al. 199670 kDa protein 2 (HSP70-2) leads to spermatogenic arrest at metaphase stage in knockouts. Cell Cycle Regulation in Mammalian Germ Cells 351Table ... However, understanding cellcycle regulation in mammalian germcells has considerably lagged behind that of the mitotic cell cycle. Challengesto investigations of meiosis in mammals have included the...
... variant containing the DNA binding motif but not the pRb-binding ortransactivation domain or introduction of a competitor plasmid containingmultiple E2F binding sites, preventing the binding of E2F ... Thepocket-domain is responsible for binding to proteins containing LXCXE motifs while thepocket-domain and the C-domain are involved in binding E2F proteins. Mdm2 (as wellas c-Abl) binds to the C-domain. ... overlapping roles in controlling cell cycle exit upon growth-inhibiting signals. Only ablation of all pocket proteins fullyalleviated a cellcycle arrest upon serum withdrawal, cell- cell contact inhi-bition,...
... for linking cell cycle regulation to internal and external signaling pathways via regulated protein kinaseactivities.1IntroductionThe importance of ubiquitin-mediated proteolysis for cellcycle ... remain to be elucidated (Kominami et al.1998; Suzuki et al. 2000). Interestingly, there are three classes of related F-box proteins in both yeast and mammalian cells that are involved incell cycle control. ... the spindle assembly checkpoint, which will be discussed in greaterdetail below, maintains Cdc20 in an inactive state until bipolar attachment ofThe ubiquitin-proteasome pathway incell cycle...
... HeLa cellcyclein a site-specific manner.Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4To determine which kinase is involved in the phos-phorylation of MCM4 in phases ... ofDNA in a single cell cycle. Inactivation of CDK1 leadsto re-replication of DNA in eukaryotic cells includinghuman cells [7]. Targets of the kinase in the regulationof DNA replication include ... proteins onlicensed chromatin in the G1phase was detached fromchromatin. Phosphorylation of MCM4 and other fac-tors may be involved in stronger binding of MCM4protein to chromatin in the...
... protein in CHO-BP1a cells, p21 and p27 proteins were markedlyinduced, as shown by immunoblot analysis (Fig. 4B). In accordance with the induction of these cdk inhibi-tors, SREBP-1a-expressing cells ... activation of acyclin E–cdk2 complex during the G1phase of thehuman cell cycle. Science 257, 1689–1694.20 Weinberg RA (1995) The retinoblastoma protein and cell cycle control. Cell 81, 323–330.21 ... (1998) Characterization of two Chinese hamsterovary cell lines expressing the COOH-terminal domainsof sterol regulatory element-binding protein (SREBP)-1. Cell Struct Funct 23 , 187–192.33...
... colony-forming cells was determined as indicated in the methods section.Fig. 6. Effect of fatty acid-unsaturation-inducer dioctylphtalate onh-Bax-induced cell death kinetics. WtB1cellsweregrowninacom-mercial ... measured inYeast Nitrogen Basemedium (7.05 ± 0.05; n ¼ 3). Kinetics of Bax-induced cell death varied accordingly to the extent of Bax-inducedmitochondrial lipid oxidation. In the minimal mediumwithout ... dioctylphtalate, Bax-induced cell death kinetics wasmuch slower than inYeast Nitrogen Base medium (Fig. 6).The addition of dioctylphtalate restored cell death kineticscomparable to experiments in Yeast...
... trends in the Stanford yeast cell- cycle data to uncover genome-wide oscillations in expression in 4500 of the 6178gene expression profiles. These were typically of cell- cycle or half -cell- cycle ... 40-min cycles (Fig. 3A,C)are similar to one another in forming a bowl or conicalshape around the steady state. In other projections ofthe phenelzine-treated cells, the increase incycle ... the epithelial cells of thegastro-intestinal tract are similar – if on different timescales. In the gastrointestinal tract of mammals the cell- cycle time of a particular cell is in the range5–10...
... colony as a whole.An intriguing candidate is the lectin-like proteininvolved in flocculation, a cell wall protein that bindsto mannose chains on the surface of other cells toprovide the physical ... cultured yeast cells in terms of transcriptional activity led to thenotation that collective modes of whole population ofcells indeed in uence transcriptional machinery. Thissynchronization, having a ... unity indicates the non-noisy character of theextracted modes, pointing to the highly coordinatedgene expression behaviour of these cells.HeLa cells display whole genome cell cycle independent...
... cellcycle [11–15]. Cer-tain transacting proteins such as HuR were proposedas regulators of changes in mRNA stability during the cell cycle [15]. In this context it is of interest that duringvertebrate ... elutriated cells. The cell cycle distribution of the cell fractions is shown in Fig. 4.ABCDFig. 1. Steady-state levels of cyclin mRNAs in enriched cellcycle fractions of mouse EL4 cells. Cells ... anincrease in late S and G2⁄ M phase confirming previousobservations [26,27], but no cell cycle- dependentchange in stability. The cyclin E1 mRNA wasincreased in the G1phase of the cell cycle, ...