cd4 t helper cells
Báo cáo y học: "Between adaptive and innate immunity: TLR4-mediated perforin production by CD28null T-helper cells in ankylosing spondylitis" pptx
Ngày tải lên :
09/08/2014, 07:20
... of TLRs as an alternative stimulatory pathway on CD4+ CD28null T cells Patients and methods A total of 90 consecutive patients with spondyloarthropathies, 72 patients with RA and 64 age-matched ... n.d., not determined production of INF-γ and T- helper- 1 accentuated immune responses [13,14] After in vitro activation, mouse CD4+ T cells express TLR3 and TLR9, and treatment of these cells with ... induced on CD4+ CD28+ T cells nor on CD8+ T cells after incubation with TNF-α (data not shown) To examine the effects of TNF-α blocking treatment on the expression of PRRs on CD4+ CD28null T cells in...
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Báo cáo y học: "Polarized subsets of human T-helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts" doc
Ngày tải lên :
09/08/2014, 07:20
... immunosorbent assay T cell membranes were solubilized in 1% NP40 to detect cytokine content Statistical analysis Student's t test was used with two-tailed P values or Mann– Whitney U test using StatView ... by fibroblasts was shown to depend on the type of Th cell that stimulates them This is consistent with the capacity of fibroblasts to activate flexible patterns of cytokine production readily ... but was strongly induced by Th2 and to a lesser extent by Th1 cells – an effect due to cytokines (IL-1 and TNF-α) shared by the two T cell subsets This indicates that the biological activities...
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Báo cáo y học: "CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individual" docx
Ngày tải lên :
13/08/2014, 13:22
... Competing interests The author(s) declare that they have no competing interests Authors' contributions All authors participated in the design of the study KA conducted the patient contact and ... [18] Three of the allergic patients had allergic asthma The allergic subjects received no corticosteroid treatment for three months prior to the study The asymptomatically sensitized and healthy ... characterization, cell isolation and stimulation assays MH conducted the flow cytometry and analyzed the data All authors contributed towards the manuscript preparation with MH as the main author...
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Báo cáo y học: "Differentiation of naive CD4+ T cells towards T helper 2 cells is not impaired in rheumatoid arthritis patients" pps
Ngày tải lên :
09/08/2014, 01:23
... activity [17,20,21] Together these data suggest that RA patients may benefit from therapies aimed at the regulation of the Th cell balance towards Th2 cell activity It also implies that intrinsic ... contribute to this relatively enhanced peripheral Th2 activity in RA patients compared to healthy controls The reduced capacity of Th2 cells to migrate to the arthritic sites could subsequently ... RA patients than for healthy controls Although we show that IL-7, and in particular the combination of IL-7 and IL-4, increased Th2 activity rather than Th1 activity of naive CD4+ T cells, this...
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Báo cáo y học: " Expansion of CD4+CD25+ helper T cells without regulatory function in smoking and COPD" potx
Ngày tải lên :
12/08/2014, 13:22
... confirmed in longitudinal studies These data therefore indicate the expansion of a T cell population without a regulatory function, which may contribute to the persistent cytotoxic T cells responses ... supports a potential involvement of the acquired immune response in the pathogenesis of COPD To further evaluate the role of regulatory T cells in COPD and to clarify whether CD4 + CD25 bright cells ... fluorescence intensity, is associated to both current smoking and COPD (Figure 2b) Even though the number of patients in the present study is rather small, the data are consistent with previously...
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Báo cáo y học: " Antigen-sensitized CD4+CD62Llow memory/effector T helper 2 cells can induce airway hyperresponsiveness in an antigen free setting" pptx
Ngày tải lên :
12/08/2014, 18:21
... cells that are not stimulated with antigen does not alter the intrinsic AHR [39] The latter finding is strongly supported by the present findings that antigen-stimulated cells mediated AHR induction, ... OVA stimulation potentiates the intensity of transfer-mediated AHR In another experiment, OVA-sensitized spleen cells were stimulated with OVA in vitro and then transferred This treatment increased ... response, but not Th1 type Antigens that elicit Th2 type response, but not Th1 type response, induce transfer-mediated AHR (A) Antigens that elicit Th2 type response induce transfer-mediated AHR...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... NK cells initially-obtained their name due to their natural cytotoxicity against tumor cells requiring no prior sensitization, unlike T cells [4] It is well established that the cytotoxicity ... cytotoxic against the K562 cell line and that 3a-G1 doesn 't affect their cytotoxicity when compared with untreated cells [see Additional file 1] Contrary to expectation, we could not demonstrate ... interacts with CD4+ T cells We believe that this interaction might drive the inhibition of CD4+ T cell proliferation observed not only among PBMCs but also when pure CD4+ T cells were stimulated...
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Báo cáo y học: "Resistance to IL-10 inhibition of interferon gamma production and expression of suppressor of cytokine signaling 1 in CD4+ T cells from patients with rheumatoid arthritis" ppsx
Ngày tải lên :
09/08/2014, 01:24
... phosphorylation and activation of the latent transcription factors R571 Arthritis Research & Therapy Vol No Yamana et al Figure (a) control RA HC p-Tyr-STAT3 HC STAT3 p-Tyr-STAT1 STAT1 (%pSTAT3/STAT3) ... p-Tyr-STAT3 STAT3 IL-10 (ng/ml) 10 HC PB RA ST CD4+ T cells CD4+ T cells of IL-10-mediated (RA) STAT3 in CD4 T cells from (a)transcription (STAT) and from healthy controls (HC) patients with ... phosphorylation was expressed as % total STAT3 protein (c) STAT3 phosphorylation in ST CD4+ T cells from RA patients Representative results of STAT3 phosphorylation in CD4+ T cells from three synovial tissue...
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Báo cáo y học: "The p38 mitogen-activated protein kinase signaling cascade in CD4 T cells" docx
Ngày tải lên :
09/08/2014, 07:20
... cascade This deficiency might contribute to the higher activation threshold of naive T cells than that of memory T cells In addition to co-stimulatory molecules, some cytokine receptors can activate ... derived from atopic asthmatic patients partly inhibited the expression of IL-5 but did not alter that of IL-4 [68] Together, these data clearly indicate that the role of p38 MAPK in Th2 cytokine expression ... proteins to TTP, thereby withholding TTP from the mRNA degradation machinery [97,98] However, it should be noted that the interaction of 14-3-3 proteins with TTP and its role in mRNA stabilization...
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Báo cáo y học: "Are CD4+CD25-Foxp3+ cells in untreated new-onset lupus patients regulatory T cells" doc
Ngày tải lên :
09/08/2014, 14:22
... patients correlated with disease activity and that the cell number significantly decreased after glucocorticoid treatment [2] Whether these cells are Tregs or activated Teffs remains to be determined ... in a heterodimeric complex with TSLP receptor [15] TSLP-activated dendritic cells might participate in the homeostatic maintenance of CD4+ and development of Tregs in thymus [16] In this study, ... in CD4+ CD25- T cells It is also important to note that, although the CD25low population lies adjacent to CD25- on a FACS plot (as shown in Figure 2), they belong to two different cell entities...
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Báo cáo y học: " Heterogeneity of human effector CD4+ T cells" doc
Ngày tải lên :
09/08/2014, 14:22
... cells upon stimulation by IL-12 activates the signal transducer and activator of transcription (STAT)-1 in the naive CD4 T cells Activated STAT-1 upregulates T- box expressed in T cells (Tbet) expression, ... Th17 cells can shift to Th1 cells Among different CD4+ effector T cells, the Th1 cells appear to be the more stable Although this complex situation makes it more difficult to extrapolate the role ... Moreover, CD4+ Th cells seem to exhibit a great plasticity not only in the context of effector responses, but also of regulatory responses Th2 cells can shift to Th1 cells or to Th9 cells, whereas Th17...
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Báo cáo y học: "Evidence that Gag facilitates HIV-1 envelope association both in GPI-enriched plasma membrane and detergent resistant membranes and facilitates envelope incorporation onto virions in primary CD4+ T cells" doc
Ngày tải lên :
12/08/2014, 04:21
... respectively As shown in Figure 2, L30E mutation in Gag restricted envelope association with DRM fractions of CD4+ T cells in contrast to the wild type Our results were further substantiated by the ... compartment As shown in Figure 1A, due to L30E mutation in Gag, Env failed to recruit CD59 in contrast to pNL4.3 wild type Our data indicate that the mutation in Gag MA region (L30E) restricts the ... post-translationally modulated in the Golgi apparatus, traverses into the secretory pathway towards assembling onto budding virions We envisage that Gag by acting as cargo transport intermediate...
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Báo cáo y học: "NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigenspecific CD4+ T cell polarization" pot
Ngày tải lên :
12/08/2014, 11:22
... Student’s t test Hodgkins et al Respiratory Research 2010, 11:102 http://respiratory-research.com/content/11/1/102 post administration of DT in CD11c-DTR Tg+ mice We next set out to test whether ... of total lung cells Thus, it is possible that this depletion minimizes the initial number of naïve T cells activated, but that after repeated exposure to antigen, clonal expansion allows for the ... subsets The myeloid DC subset is attributed with T cell stimulatory capacity, having the ability to induce Th1, Th2, or Th17 type responses [19], as well as non-inflammatory T regulatory (Treg)...
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Báo cáo y học: " Natural killer cells control a T-helper 1 response in patients with Behçet''''s disease" doc
Ngày tải lên :
12/08/2014, 12:20
... immunoblotting for the detection of phosphorylated Stat4 (pStat4) and total Stat4 (b) Phosphorylation status of Stat4, which is expressed as the ratio of the intensity of pStat4 to that of total Stat4, ... was semiquantitatively analyzed using the image/J® software The phosphorylation status of Stat4 was expressed as the ratio of the intensity of phosphorylated Stat4 to that of total Stat4 IFNγ expression ... but the cytotoxic activity was the same among the NK cells of iBD patients, aBD patients, and healthy controls The reason for this inconsistency is unknown, but the cytotoxic activity of NK cells...
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Báo cáo y học: "Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis" docx
Ngày tải lên :
12/08/2014, 14:20
... of TIM-3, compared to healthy controls Both these factors may contribute to the characteristic Th1-type inflammation Differences in the exact type of antigen(s) presented in the respective patient ... Institutet, that were distributed to Johan Grunewald, Anders Eklund and Jan Wahlström and were important for the study design, data collection, analysis and interpretation of data Statement on ... subgroups (data not shown) TIM-1 was not detectable on T cells from either patients or controls Discussion In this study, we demonstrate a decreased mRNA and protein level of TIM-3 in BALF CD4+ T cells...
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Báo cáo y học: "Defective response of CD4+ T cells to retinoic acid and TGFb in systemic lupus erythematosus" ppt
Ngày tải lên :
12/08/2014, 17:22
... indicating that treatment was not the main cause for low CD4+ T cell counts However, treatment was associated with a further decrease in the percentage of CD4 + T cells (untreated patients: 11.51 ... albeit incomplete, and have concluded that these cells represent an attempt to control active autoimmune activation [14] The size of the Treg compartment results from the combined contribution ... suggested that CD25- Tregs correspond to activated T cells without suppressive activity [13] The other group working with treated patients has shown that the CD25- Tregs retain a suppressive function,...
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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
Ngày tải lên :
12/08/2014, 23:20
... the up-regulatory effect of NF-κB with regard to HIV-1 transcription and the potent induction of this transactivator by TLR2 stimulation, we thought that the TLR2-mediated augmentation in de novo ... monitored using the fluorescent cytotoxic MTS assay Cell viability was not affected by the studied TLR ligands used at concentrations known to modulate the DC-mediated transfer of HIV-1 (data not ... efficient than TLR5, and stimulations as evidenced by the higher production of IL-6, TNF-α and RANTES Knowing that TLR4 stimulation can activate pathways resulting in both NF-κB activation and secretion...
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Báo cáo y học: " On the steps of cell-to-cell HIV transmission between CD4 T cells" pptx
Ngày tải lên :
12/08/2014, 23:22
... entire synaptic buttons [20] Although this latter observation may be consistent with trogocytic events, we have described that trogocytosis occurs at the VS from the target to the infected cells, ... transferred to uninfected cells by trogocytosis through the formation of tethering tubes, potentially allowing for viral RNA to enter the cytoplasm of target cell (without exposure to the extracellular ... transfer to primary CD4 T cells An alternative approach to characterizing transport phenomena at the VS is the evaluation of the transfer of membrane lipids from MOLT infected cells to target...
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Báo cáo y học: " CD4+CD25+ T regulatory cells from FIV+ cats induce a unique anergic profile in CD8+ lymphocyte targets" pps
Ngày tải lên :
13/08/2014, 01:20
... lymphocyte targets following their interaction with activated Treg cells To further define the relationship between activated Treg cells and CD8+ targets in FIV infected cats, we asked if Treg cells ... contained in this manuscript and drafted the manuscript WT assisted with study design, data interpretation and manuscript revisions MT assisted with study design, data interpretation and manuscript revisions ... CD8+ T lymphocyte targets without these cells exhibiting regulatory function; however, the function of Foxp3 in these target cells in unclear [46-48] Further investigation is needed to clarify the...
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Báo cáo y học: "Distinct host cell proteins incorporated by SIV replicating in CD4+ T Cells from natural disease resistant versus non-natural disease susceptible hosts" docx
Ngày tải lên :
13/08/2014, 01:20
... infection that primarily targets T cells prompted Yeung et al [29] to utilize the JURKAT cell line These authors capitalized on the availability of a shRNA library that targets 54,509 human targets ... rev, tat and nef) Please note that p27 was not detected in the database search, which we reason is due to the fact that trypsin was used to fragment the proteins, and p27 does not contain a trypsin ... substrate specificity to the 26 S complex It has been demonstrated that the 26 S protease regulatory subunit (MSS1 protein) enhances the HIV1 ‘tat’-mediated transactivation [33] and associates...
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