cation of a clone from a gene library
Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx
... TACAAT TATAAT TAAAAT TACTAT TATAAT TAATTT TATAAT TATAAT + – – + – + + CIRCE – This work [10] [9] [61] [62] [63] [38] [64] [37] Fig SDS/PAGE analysis of expression and puri cation of the recombinant ... alkaline phosphatase Materials and methods Bacterial identi cation and culture conditions DNA manipulation and sequence analysis Plasmid DNA preparation, puri cation of DNA from agarose gel, and restriction ... spoIIG amyR rA consensus r70 consensus TTAGACA TTAGACA TTGTACA TTGTTAC TTGTATT TTGACAG TTGTTTT TTGACA TTGACA 17 17 17 20 17 21 16 16–18 16–18 TTTG TTTG GTTG TATG TATG CTTG TGTG TNTG – TACAAT TACAAT...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... cluster of S alboniger [6] They were accordingly named ataP3, ataP5, ataP4, ataP10 and ataP7 The two additional ones were named ata12 and ataPKS1 (Figs and 3) All shared a codon usage and a G+C ... complementation assays The ataP5, ataP4 and ataP10 genes were also independently inserted in the pIJ702 vector and the resulting plasmids pA 2A5 , pA 2A4 and pA 2A1 0, respectively (Materials and methods), ... in A2 0 1A biosynthesis In contrast, upstream of ard1 there are six contiguous coding sequences with identical orientation (Figs and 3A) Of these, five (ataP3, ataP5, ataP4, ataP10 and ataP7) are...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG Ó FEBS 2002 CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig ... CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and TRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢), respectively The PCR ... 32281–32287 31 Itadani, H., Nakamura, T., Itoh, J., Iwaasa, H., Kanatani, A. , Borkowski, J., Ihara, M & Ohta, M (1998) Cloning and characterization of a new subtype of thyrotropin-releasing hormone...
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Báo cáo khoa học: Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library pdf
... folded GABARAP [22] The addition of CRT to GABARAP resulted in the disappearance of GABARAP resonances, a clear indication of binding (Fig 3B) Only weak amide signals for a Gln ⁄ Asn side chain and ... et al (2001) Functional specialization of calreticulin domains J Cell Biol 154, 961–972 Harada K, Takeuchi H, Oike M, Matsuda M, Kanematsu T, Yagisawa H, Nakayama K-II, Maeda K, Erneux C & Hirata ... interact with CRT By contrast with GABARAP, no quantitative data are available for these interactions Most importantly, the interaction of CRT with GABARAP occurs with natively folded GABARAP This...
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Báo cáo khoa học: Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H pot
... Muroya A, Tsuchiya D, Ishikawa M, Haruki M, Morikawa M, Kanaya S & Morikawa K (2001) Catalytic center of an archaeal type Ribonuclease H as revealed by X-ray crystallographic and mutational analyses ... Structure of ribonuclease H phased at A resolution by MAD analysis of the selenomethionyl protein Science 249, 1398–1405 Ishikawa K, Okumura M, Katayanagi K, Kimura S, Kanaya S, Nakamura H & Morikawa ... 5¢-TTCAAGAATTCTCATGTTTTGAC -3¢ for the 5¢-primer, 5¢-CGCGTCGACACTAACAG GGCTGATTGACGAGTC-3¢ for the 3¢-primer, 5¢TCTACCAGAG ATG TCGACATTATCGGTTGTG-3¢ for the 5¢-fusion primer and 5¢-TAATGTCGA CAT CTCT GGTAGACTTCCTGTAA-3¢...
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Báo cáo khoa học: Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics pdf
... according to the manufacturer’s instructions The cDNA was amplified with Lipases in papaya latex the primers 5¢-ACTCAAATCACTAGTATTCTTCCACCA-3¢ and 5¢-CATTTGAACATAAACATGAACAAATAAGTT-3¢ and the following ... percentage of release from position was calculated as follows: area fatty acid ⁄ (area fatty acid + area lysoPtdCho) Pancreatic PLA2 was used as a positive control, and T lanuginosus lipase as a PLA1 ... close relative of C papaya is V heilbornii (mountain papaya or babaco), formerly known as Carica pentagona [30] The Vasconcellea and Carica genera are close enough that hybrids can be obtained under...
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Báo cáo khoa học: Identification of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebrafish Molecular cloning, expression, characterization and ontogenic study ppt
... 5¢-TATGTAGGAGCTACAAGAAACATTGAAGGC-3¢ 5¢-CAATTCTTACTAGCTGCAGGGAGGGTTGGT-3¢ 5¢-GAATTGGCCCTAATTTGCACATTAAAGATA-3¢ 5¢-GCCTGAAGTTTTTGGTTCACAGTGAAATTT-3¢ 5¢-ACACTCTGAAGGGGAATTAGGATTAAGAAA-3¢ 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ ... 5¢-CTGACTATACAAGGCTGTGTGCCACAAAAC-3¢ 5¢-GAAAACACATCACGTACCCTCCCTCTCTGCG-3¢ 5¢-ACATCATGGTATATTATTCATTTAGCTGACACTTT-3¢ 5¢-ATGGATGAGGAAATCGCTGCCCTGGTC-3¢ 5¢-TTAGAAGCACTTCCTGTGAACGATGGA-3¢ 3835 A novel ... ATGAGCCGGAGAACAAGCGAAACT-3¢) and antisense (5¢-CGCGGATCCTTATATAGTGAAGCGTATTGGAAG FEBS Journal 272 (2005) 3828–3837 ª 2005 FEBS S Yasuda et al A novel zebrafish cytosolic sulfotransferase AGGACA-3¢)...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... recombinant NaoA after Sephadex G75 chromatography; lane 5, purified recombinant NaoA after DEAE-Sepharose Fast Flow chromatography; lane 6, standard molecular mass markers (phosphorylase b, 97 kDa; ... this gene was designated naoA (nitroalkane-oxidizing enzyme gene) Expression of naoA in E coli To study the function of the naoA gene, it is necessary to obtain an adequate amount of NaoA protein ... metal ions may also act in other ways We conclude that NaoA is a nitroalkane dioxygenase-like enzyme rather than a nitroalkane oxidase Its characteristics are not identical with those of any...
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx
... primers A3 were as follows: 5¢-GCCGGATCCCCGATGACGATGACAAG GATGGATATATAAGA-3¢ as forward primer containing a BamHI restriction enzyme site (underlined) and corresponding to five codons encoding an enterokinase ... such as A (Ala) or D (Asp) Only AaHIT4 and BmKAS, a specific anti-insect toxin, also contained a Tyr residue at this position AaHIT4, the unique anti-insect toxin also has a toxic effect on mammals ... These data indicated that rBmKIM had toxicity to both mammals and insects, though the toxicity was at a lower level Assay of antiarrhythmia activity Table illustrates the effects of rBmKIM on aconitineinduced...
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Báo cáo sinh học: " Identification of a differentially expressed gene, ACL, between Meishan · Large White and Large White · " pps
... TCCACCACCCTGTTGCTGTA CCCTTTGCCATTGTTATA TCAGAGGTCGGTCAAACG ACGGGCAATACCCTCTGG AGGACTCGTCGGGAAGCA CCACCAGTCCTGATGCCA AGCCTTCAAGCGGGGAG CAAGCTCTTGAAAGGCATCG AGAGGGCTTCCTGGCTCA AACGCAGAGTACGCGGG CAGCCAAGGGTGGTCCTGC ... ACCACAAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA TCTGGGAGGTGTCAACGAG GGTCTTGGCATAGTCATAGGT GCTACGCGTTCAGCACTATCAGATCGGG GCTACGCGTCCTTCCTAGCCCCACCT GCCACGCGTATCTATTAGCCTCGTCCCAC GATACGCGTCAGCCCGCCACATCTCAG GATACGCGCATAGCCCAGCCCATCTC ... CAGAGTACTTTTTTTTTTTTTTTT AGCAGGGGCTGTATCGTC NGTCGASWGANAWGAA GTNCGASWCANAWGTT WGTGNAGWANCANAGA NCAGCTWSCTNTSCTT CTGCTCTCTACGAAAGGCCGTGC CCCAACTCGCCGCCTACCTTCC TCGCCGCCTACCTTCCGGAGCGC ACCACAAGTCCATGCCATCAC...
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Báo cáo sinh học: "Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus" pps
... and µL was used to inoculate a new overnight culture This was repeated on day 2, 3, 4, and until 100 generations had passed BAC DNA from each clone was prepared as above on day and NotI and HindIII ... libraries, Genomics 52 (1998) 1–8 [18] Rogel-Gaillard C., Bourgeaux N., Billault A. , Vaiman M., Chardon P., Construction of a swine BAC library: application to the characterization and mapping of ... Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector, Proc Natl Acad Sci USA 89 (1992) 8794–8797 [21] Suzuki K., Asakawa S.,...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... gtgactgcag gaaggggatc cggctgctaa caaagcccga 280 281 aaggaagctg agttggctgc tgccaccgct gagcaataac 320 321 tagcataccc 361 TTTTTGCTGA cttggggcct AAGGAGGAAC ctaaacgggt TATATCCGGA cttgaggggt TATCCCGCAA ... MCoTI-II gene cttaaggtatactcgccgtcgcgtaccgccgcacacgggcttt gaattccatatgagcggcagcgatggcggcgtgtgcccgaaa M S G S D G G V C P K taagacttttttacggcggcgctatcgctaacgggcccgcgc attctgaaaaaatgccgccgcgatagcgattgcccgggcgcg ... Gtttttgctt ggatcccagg ttgttgtaca gaatgctggt 120 121 catatgagcg gcagcgatgg cggcgtgtgc cgaaaattct 160 161 gaaaaaatgc cgccgcgata gcgattgccc gggcgcgtca 200 201 tttgccgcgg caacggctat tgcggctaac tcgagcccgg 240...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... TCATCAT-3¢; rOMM-64-IV, 5¢-CGCGGATCCGCCCCT GTTAATGATGGAACC-3¢ and 5¢-CGCCTCGAGCTAA GAAGACTGGGCTGCCAG-3¢; rOMM-64-V, 5¢-CGCGG ATCCAGGCAAGATTTTAAGCATCCA-3¢ and 5¢-CGCC TCCACCTAAGAGGCATCCTTGTCCAC-3¢; ... 5¢-CGCCTCCA CCTAAGAGGCATCCTTGTCCAC-3¢; rOMM-64-II, 5¢CGCGGATCCACCGTAGACACTTATGATATA-3¢ and 5¢-CGCCTCGAGCTAAGAGTCAGCTTGCACGTC-3¢; rOMM-64-III, 5¢-CGCGGATCCGCTGATGTGACCAGT GATGAC-3¢ and 5¢-CGCCTCGAGCTATTTGGGCTCTT ... digestion of genomic DNA contamination in the total RNA was confirmed by lack of ampli cation of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢)...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... N-terminus of P40 as it appears in protein extracts of M pneumoniae Determination of the N-terminus of P40 by Edman degradation failed due to the limited amount of starting material An alternative ... processing of the gene product of MPN 142 Cleavage into P40 and P90 takes place after amino acid 454 The N-terminus of the mature P40 starts with amino acid 26 The molecular mass of about 36 000 Da of ... FEBS I Catrein et al nated sulfonic acid group and lost during fragmentation, which causes neutralization of the b-fragments The formation of sodium and potassium adducts was possibly a general property...
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Báo cáo khoa học: Identification of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx
... glycoforms were also observed for strains 425/93 and 1000 (Table 1) Core oligosaccharide from the 1000 galE mutant strain was also prepared and examined by MS A range of molecular masses was found ... presence of an additional phosphate group (1032), and additional PEtn moiety (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group and two additional ... liberated oligosaccharide and an associated extracellular polysaccharide Carbohydr Res 279, 227– 244 24 Aspinall, G.O., Monteiro, M .A & Pang, H (1995) Lipo-oligosaccharide of Campylobacter lari...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... percent of the amount of NADH used in our standard NR assay, and 60% of the amount of ATP in a GS assay, was converted into AMP within 10 at 30 °C by an amount of a Mono Q fraction that appeared ... in a 100-lL incubation at 30 °C for 10 min, using the amounts and concentrations of ATP and NADH used in standard GS and NR assays, respectively Data are presented as mean ± SEM Cofactor AMP generated ... trichloroacetic acid The A5 04 was measured and the c-glutamyl hydroxamate produced was quantified using commercial c-glutamyl hydroxamate as standard Control assays were performed in the absence of ATP...
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Báo cáo khoa học: The identification of a phospholipase B precursor in human neutrophils doc
... the appearance of a significant deacylation activity Any bacterial contamination of our protein preparations that might be responsible for activation of the PLB precursor at prolonged storage was ... staining as described in Materials and methods Marker proteins and their corresponding molecular masss are indicated in lane Lane 2, material from the acid extracts of granules; lane 3, material ... and bacterial, protease contamination Protein concentration was determined with a Bio-Rad protein assay kit using BSA as a standard according to the manufacturer’s protocol Any bacterial and protease...
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Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx
... GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGG Ó FEBS 2003 3020 P Prijatelj ... GGGCaagcTTCCCCGTCTCCtCCAGGATCATCtTCCCG GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG GACTGCAaCCCCAAAtCGGACAG CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC GGAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC ... each ampli cation primer, 400 lM of each of the four deoxyribonucleoside triphosphates, Taq DNA ligase buffer (New England BioLabs) and 100 ng of template DNA Manual hot start ampli cation was...
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Báo cáo khoa học: Identification of a glycosphingolipid transfer protein GLTP1 in Arabidopsis thaliana ppt
... GAATTCAGAGAAAGAGATACGAGATGGA-3¢) and U660033Not (5¢-TCATAAGGCGGCCGCCTACATCG ATCTTAATCTGCTCAA-3¢) A fragment carrying At3g21260 cDNA was amplifed from A thaliana RNA by RT-PCR RNA was isolated from ... GGTTTCTAAACCAACACGT-3¢) and GLTP1PRONBAM (5¢-CTCCTTGGATCCGCCTGAGAATTGAAAAA GGTGGG-3¢) A 1.3 kb fragment carrying the At1g21360 promoter was amplified using primers 21360PRUXBA (5¢-AACGATCTAGATTAAGAATGTAATCACATTAGG ... Histochemical GUS activity assays A 1.8 kb DNA fragment carrying the At2g33470 promoter was amplified from the A thaliana Col-0 genome using primers GLTP1PROUXBA (5¢-AGACTGCTCTAGAATG GGTTTCTAAACCAACACGT-3¢)...
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Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG CTGGTGTGGGCAGCAGAACTCAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGAGTTCTGCTGCCCACACCAG CTGGTGTGGGCAGCAGAATACAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGTATTCTGCTGCCCACACCAG ... Sense Antisense Sense Antisense Sense Antisense Sense Antisense CTGGTGTGGCCCACAGAACTCAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGAGTTCTGTGGGCCACACCAG CTGGTGTGGCCCACAGAATACAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG ... LumiGLOTM was from Cell Signaling (Beverly, MA, USA), and AdvantageÒ polymerase mix was from Clontech (Palo Alto, CA, USA) All other reagents were of the best quality and commercially available Expression...
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