calix 4 arene derivatives binding stoichiometry and spectroscopic evaluation of the host guest recognition mechanism
Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
Ngày tải lên :
07/03/2014, 17:20
... (UAS) and is characterized by two imperfect palindromes of 16 bp and a spacing of between 29 and 42 bp between their centres (Fig 1A) [20] The regions covered by XylR on the Pu and of HbpR on the ... impaired in binding of the R13 region Both of these patterns contributed to the loss of binding contacts of mutant 72 Construction of a DA HbpR derivative In order to study whether the A-domain ... described in the main text Relevant restriction sites and the direction of transcription for the hbpR and the luxAB genes are indicated (A) (B) Detail of the region of the calmodulin -binding protein...
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Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa pdf
Ngày tải lên :
16/03/2014, 18:20
... due to the amount of Pr present in the Pfr-enriched form The observed Pfr ground state is the opposite of that used by almost all other known members of the classic phytochrome family [12, 24] More ... photochemical reaction, as a H 247 Q mutation resulted in a spectral shift of the Pr and Pfr forms Therefore, the BLD may play a role in stabilization and co-ordination of the chromophore and possibly its covalent ... and plant phytochromes To investigate the role of this histidine residue, a H 247 Q mutant was generated H 247 Q was able to form a photoconvertible holoform with blue-shifted extrema (6 94 and 746 ...
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Báo cáo hóa học: " Development and preliminary evaluation of the participation in life activities scale for children and adolescents with asthma: an instrument development study" pptx
Ngày tải lên :
18/06/2014, 22:20
... SD 49 1 549 696 1.737 49 5 636 798 929 362 356 366 9 14 3 34 301 277 716 t df P -.0 74 -1.975 -2.079 -1.569 244 244 99† 102† 941 049 040 * 120 *p-value significant < 05 †Levene's Test for Equality of ... PI After being informed of the purpose and nature of the study, requirements and responsibilities of subjects, and risks and benefits, families agreeing to participate in the first two studies ... support for the qualitatively-derived theoretical underpinnings of the instrument The PLA contributes to the advancement of science by offering a tool to measure what is hypothesized to be the primary...
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báo cáo hóa học:" A Process and Outcomes Evaluation of the International AIDS Conference: Who Attends? Who Benefits Most?" docx
Ngày tải lên :
20/06/2014, 08:20
... (1, N = 242 8) = 171.35, P < 01], and the majority of these delegates were from Asia [2 (4, N = 247 2) = 38.31, P < 01] The majority of African respondents were from Southern Africa ( 94% ); the largest ... did not complete and hand in the questionnaire and, of those who did, 41 % did not answer the outcomes question Given the demographics of those participating in the evaluation, the outcomes are ... recommending the IAC to a peer, and the qualitative comments referring to the lack of science, the low quality of the science presentations, and the need to balance psychosocial and policy content...
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the situation of diabetes, pre-diabetes to the khmer in hau giang province and an evaluation of the efficacy of some interventional methods
Ngày tải lên :
25/07/2014, 11:36
... 10,8 49 83,1 59 0,01 41 69 ,4 59 16 ,4 50 84, 7 59 0, 34 46 78,0 59 7,9 29 49 ,2 59 0,55 25 42 ,4 59 13,3 31 52,5 59 0,01 30 50,9 59 3,3 53 89,8 59 0,01 45 76,2 59 15,1 54 91,5 59 0,12 50 84, 7 59 7 ,4 ... between demography and diabetes The prevalence of diabetes of male is 11 ,4% while that of female is 12,3%, Age group has the smallest prevalence of diabetes is 45 – 54 made up 10,2%, the highest is ... 16,5 % The prevalence of diabetes of people who live in the countryside is 12,7 % The prevalence of diabetes of farmers is 13,1%, that of hired labor is 13,3% The prevalence of diabetes of literacy...
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Báo cáo y học: "oronary artery bypass surgery and longitudinal evaluation of the autonomic cardiovascular function" pps
Ngày tải lên :
12/08/2014, 20:21
... were calculated: the standard deviation of all R–R intervals; the standard deviation of the averages of the R–R intervals calculated in segments; the percentage number of pairs of adjacent R– Available ... infarction within the CABG and CAD groups The minimum necessary sample size was determined by setting the statistical power to 0.8 and the alpha error to 0.5 On the basis of the results of the R–R interval ... carries the influence of both sympathetic adrenergic and parasympathetic modulation, and the ratio between LF and HF components should indicate sympathetic modulation, or the reciprocal relation of...
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![Báo cáo khoa học: Calix[4]arene methylenebisphosphonic acids as inhibitors of fibrin polymerization doc](https://media.store123doc.com/images/document/14/rc/tp/medium_tpb1394040066.jpg)
Báo cáo khoa học: Calix[4]arene methylenebisphosphonic acids as inhibitors of fibrin polymerization doc
Ngày tải lên :
06/03/2014, 00:21
... suggesting that the inhibitory effect of C-192 may be a result of the blocking of site ‘A’ by this calixarene The results obtained in the present study suggest that the other types of calixarenes noted ... The addition of calixarene C-192 to the mobile phase decreased the capacity factor, k¢, of the guest molecules (Table 2) This confirms the formation of inclusion host guest complexes There is linear ... values of compounds that cause 50% inhibition of the maximum rate of the polymerization of fibrin produced in the fibrinogen + thrombin reaction Fig The dependence of the PT and APTT ratios on the calixarene...
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Báo cáo khóa học: Metal-binding stoichiometry and selectivity of the copper chaperone CopZ from Enterococcus hirae pot
Ngày tải lên :
23/03/2014, 12:20
... with the concentration of copper, as could be expected with a change in the hydrophobicity of the local environment of Tyr63 and/ or a contribution of the binding of the copper to the thiolates of ... greatly inuence the stoichiometry by changing the form of the complex As these compounds can compete with the protein to bind the metal ion, their inuence on the stoichiometry of the complex EhCopZmetal ... to the protein EhCopZ The stoichiometry of the metalloaded chaperone was found to depend on the experimental conditions used, especially the concentration of the protein and the presence of an...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
Ngày tải lên :
21/02/2014, 00:20
... particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes In addition, the first partial spectroscopic and redox characterization of the NrfH subunit is also ... regard, the best combination of the two subunits that match the smallest molecular mass is a2b2 Overnight incubation of the protein in this detergent led to a decrease of the area of the first peak and ... first time the isolation and biochemical characterization of D desulfuricans ATCC 277 74 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The reassessment of previous spectroscopic...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
Ngày tải lên :
21/02/2014, 15:20
... 8.386 8 .48 4 4. 347 4. 666 4. 252 4. 5 74 4.327 2.389 2.707 4. 155 1.798 2.255 1.978 2. 543 1.978 1.978 3.336 3.336 1.1 34 1.629 1.978 1.629 1.879 3.160 3.782 3.160 3.582 1.7 24 1.978 7.761 7. 043 Table ... CONH CONH¢ a b b¢ c c¢ d d¢ – – 8.9 14 8.510 4. 347 4. 779 4. 445 4. 5 04 4.295 2.397 2. 747 4. 297 1.813 2.270 1.983 2.558 1.983 1.983 3.368 3.317 1.217 1. 645 1.990 1. 645 1.879 3.160 3.705 3.160 3.591 ... 36% of the interaction of the full peptide with the binding cavity of SM3 The sizes of the surfaces were determined using distances between ligand ˚ atoms and protein atoms of less than A These...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
Ngày tải lên :
21/02/2014, 15:20
... at 41 5 and 44 2 nm, and for the oxidized form at 40 3 and 42 2 nm The b–band can be seen at 517 nm and the a–band at 547 nm and 603 nm The difference signals that can be observed between 40 0 and ... c the Soret band was reported to absorb at 41 8 nm, the b-band at 520 nm and the a–band at 550 nm [18] The deviations of approximately nm between the signals of horse heart cytochrome c and the ... different environment of the heme centers The difference signals observed at 44 2 nm and 603 nm can be assigned to the contributions of the hemes a and a3, with the position of the a-band showing a downshift...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
Ngày tải lên :
22/02/2014, 04:20
... albumins On the other hand, the direct thermodynamic and kinetic characterization of Ni(II) binding at site I in HSA and BSA was obtained These data can be very useful in further studies of the toxicologically ... complexes of GGH, where the a carbon of the His residue is the sole source of chirality [10] However, while positions of the component dd bands and of CT transitions are relatively constant, their ... ()0.72) (+0. 34) (+1.33) ()2.50) ()0.95) (+0.53) (+1 .40 ) ()2.79) ()0 .46 ) (+0 .48 ) (+0.72) ()1.99) ()0. 54) (+0 .49 ) (+0.96) ()0. 94) (+0 .40 ) (+1 .42 ) 206 DAHKam 552 47 7 315 275 561 48 5 306 270 567 48 9 308...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
Ngày tải lên :
07/03/2014, 17:20
... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... periods of and s, and then the average 15N-1H NOE was obtained Additional R1 values (20, 40 , 80, 140 , 240 , 40 0, 800, and 1200 ms with a delay of s) and R2 values (16.8, 33.5, 50.3, 67.0, 100.5, 1 34. 0, ... between hHR23B and hHR23A are shown Also, b-N and b-C are the N- and C-termini of XPCB–hHR23B; a-N and a-C are the N- and C-termini of hHR23A FEBS Journal 272 (2005) 246 7– 247 6 ª 2005 FEBS 246 9 Dynamic...
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Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx
Ngày tải lên :
07/03/2014, 21:20
... disposition of the proximal His and Asp residues, affecting the imidazolate character of the His, but also by the formation of a LS species The latter indicating the probable binding of His42 to the ... comparison of the electronic absorption and RR spectra of the wild type and Thr171Ser either in the presence of the aromatic donor BHA or at alkaline pH Addition of saturating amounts of BHA did ... impact on the properties of His170 The strength of the hydrogen bond between the proximal His and Asp residues, and thus the imidazolate character of the His is expected to modulate not only the strength...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
Ngày tải lên :
16/03/2014, 18:20
... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid ... quenching of protein Trp fluorescence and in an increase of the specific DAUDA emission after the noncovalent incorporation of the ligand Binding of DAUDA to Ag-NPA-1 caused 20% quenching of the emission ... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary...
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Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
Ngày tải lên :
16/03/2014, 18:20
... on the effect of two variables, the reaction time and the presence of chloride ions, on the stoichiometry, stereochemistry and degree of folding of the Hg(II)-MT species formed by either the binding ... Notwithstanding this, there is a minor effect of t and X at the beginning and end of the titration Thus, the addition of the first 4 6 of Hg(II) to apo-MT gives rise to Hg-MT species of comparable stoichiometry ... concerning the Zn,Hg4-aMT species, the 244 (+) nm CD band recorded after the addition of Hg(II) to Zn4-aMT under all sets of conditions not only gives a clear indication of the presence of Zn(II) in the...
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Báo cáo Y học: Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72 pptx
Ngày tải lên :
17/03/2014, 17:20
... 3.69 a 4. 62 4. 99 4. 97 3.32 4. 09 3.76 3.51 3.92 3.95 3.62 4. 16 4. 36 3.60 3.87 3.97 4. 05, 3.75 3.95, 3.73 4. 61 4. 96 4. 03 3.58 3. 74 3.70 4. 00 3 .44 3.69 3.66 a 3.87, 3.77 a a a Signals for H6a and H6b ... by the ratio of the integral intensities of the signals in the major and minor series, the average degree of glycosylation with GlcII is 75% Comparison of the 13C NMR spectra of the initial and ... part of antibodies to P penneri Absorption of P penneri O-antiserum with the alkalitreated LPS of P penneri 40 and 41 abolished binding of these antigens and signi®cantly decreased binding of the...
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Báo cáo khoa học: Spectroscopic characterization of the oxyferrous complex of prostacyclin synthase in solution and in trapped sol–gel matrix doc
Ngày tải lên :
23/03/2014, 07:20
... intermediates of microsomal P450s Oxyferrous complex of PGIS 0.00 0.0 –0.08 –0.1 360 40 0 44 0 48 0 360 40 0 44 0 48 0 Wavelength (nm) Fig Absorption spectra of PGIS and its ligand complexes in solution and ... 0.0 380 600 0.25 40 0 42 0 44 0 46 0 48 0 40 0 45 0 500 550 600 650 Wavelength (nm) encapsulated PGIS, with a peak at 43 3 nm and a trough at 41 4 nm (Fig 2C) These results indicate that the substrate access ... spectrum with the peak at 40 6 nm and the trough at 42 6 nm (lower right panel) Results of the binding of NaCN and clotrimazole to PGIS in solution and in sol–gel 360 40 0 44 0 48 0 Wavelength (nm) 0.3 1.0...
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Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
Ngày tải lên :
23/03/2014, 20:22
... identification of the minimal essential region required for the interaction with the middle domain, which mediates the dimerization of HSP90, and the other, the identification of the minimal region of the ... GRP 94- M GRP 94- M GRP 94- C vector GRP 94- C GRP 94- M GRP 94- C 2.1 100.0 1.8 2.5 Fig Suppression of the heat-induced aggregation of CS by the C-terminal domain of GRP 94 (A) One microgram of GST-GRP94M ... residues 629–732 still retained the binding activity The binding activity of residues 650– 732, i.e a further deletion of the N-terminus up to Lys 649 , was 48 .5% of the positive control (Fig 1) Thus,...
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Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx
Ngày tải lên :
30/03/2014, 11:20
... show the evolution of the spectra when the indicated number of Cd(II) was added (B) CD spectrum of the acidification of the final solution of the Cd(II) titration of Zn–ckMT, the arrow shows the ... attributed to the dissimilarity of the starting species CD spectra, and in the case of MT4, to the minor contribution at 350(+) nm assigned to the binding of Cu(I) to the bMT4 domain [16] This ... MT1 and MT4 [16–20] Spectroscopic data provided information on the number of in vivo and in vitro metal–MT generated species, their stoichiometry, and their degree of folding Additionally, the...
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