ca2 handling proteins in the av conduction axis of the failing heart
Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebrafish pptx
Ngày tải lên :
19/02/2014, 00:20
... Follistatin, Chordin and Noggin [12] These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibiting these signaling molecules from binding to their receptors in ... the extracellular space, thus inhibiting ventralizing activities Of these proteins, Chordin has a long-range effect In Xenopus, expression of the chordin gene is localized to the dorsal marginal ... mediated cleavage rate of Chordin, which may change the preference of site utilization; and (c) promotion of the dissociation of Chordin cysteine-rich (CR)-containing fragments from the ligand They...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
Ngày tải lên :
19/02/2014, 07:20
... the proteins of the outer membrane, we set out to determine what proportion was integral and to identify the major protein species To determine whether the major outer membrane proteins were integral ... to determine the proteins present in the aqueous extract (‘Aq’), the detergent phase (‘D’) and the phospholipid-rich pellet (‘P’) Arrowheads designate the size of proteins enriched in the lipid-rich ... protected in the intermembrane space (Fig 3C) An antibody recognizing the GFP tag at the C-terminus of Om14-GFP shows the electrophoretic mobility of the fusion protein is reduced, indicating a 2...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... actin-binding site located in the hnRNP U C-terminus and associate with the phosphorylated C-terminal domain of Pol II [43] Injection of a peptide acting as a competitive inhibitor of protein–protein ... presence of a p160 coactivator Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A ... enhances its activity in the presence of androgen The interaction between the N- and C-termini of the AR does not affect gelsolin FXXFF binding to AR LBD, indicating that the gelsolin FXXFF motif has...
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Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
Ngày tải lên :
07/03/2014, 21:20
... clarify the binding properties of each of these domains we generated synthetic mRNA encoding only the C1 or C2 domains Expression of only the C1 domain resulted in the dorsalization of embryos in ... underlined, and the cysteine knot is boxed Also boxed are the transmembrane domain, the ATP binding site, the L45 loop and the serine ⁄ threonine kinase domain (B) The implied amino acid sequence of CgTGFbsfR2 ... of the genes encoding Cg-BMPR1 and Cg-TGFbsfR2 is shown in Fig 2C The serine ⁄ threonine kinase domain in both proteins is encoded by two exons equivalent to kinase subdomains X and XI [22] In...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
Ngày tải lên :
16/03/2014, 11:20
... pivotal role of the UPR is to maintain ER homeostasis Therefore, the presence of mutated proteins that are unable to fold into their native conformation in the ER induces the UPR in an effort ... signal peptide in the 24-kDa a-zein and the 19-kDa a-zein endosperm storage proteins, respectively These mutant proteins are translocated into the lumen of the ER, but remain anchored to the membranes ... accumulation of unfolded proteins in the ER, BiP is competitively titrated from the luminal domain of IRE1 by the abundant unfolded proteins in the ER lumen, and IRE1 is activated Structural studies of the...
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Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
Ngày tải lên :
30/03/2014, 02:20
... structural domain constituting the Ca2+ binding motif is found in a large number of Ca2+ -binding proteins, and is also known as the EF hand [16] Proteins containing the EF hand are divided into two ... stretching of the 915 nm form shows a downshift of cm)1, indicating a stronger H-bond between the Trp)8 residue of the b-subunit and the C10a acetyl carbonyl (see below) 3D modeling of Ca2+ -binding ... parts of Fig 5A,B In both cases, the Ca2+ -binding cavities are localized in the C-termini, which comprise O of Leu)4, Ser)5, Thr)6, OD1 of Asp)7 and OD1 and OD2 of Asp)13 in Fig 5A, O of Val)3,...
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Báo cáo y học: "Expression of bioactive bone morphogenetic proteins in the subacromial bursa of patients with chronic degeneration of the rotator cuff" pptx
Ngày tải lên :
09/08/2014, 08:22
... aggrecan in this tissue (see below) Within these structures, there were significant deposits of BMP-2 and BMP-7, as visualized by indirect immunofluorescence staining The staining was concentrated in ... supraspinatus insertion site [21] These reports are not only in line with our findings, but also strongly suggest significant influence of molecular events within the subacromial bursa on the fate of the ... staining for AP Note the co-distribution of AP, both the bursa-derived intrinsic enzyme and the C2C12-derived enzyme, with the BMP deposits within the tissue supporting egg maturation [27,28], the...
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Báo cáo y học: "Novel G-protein-coupled receptor-like proteins in the plant pathogenic fungus Magnaporthe grisea" pot
Ngày tải lên :
14/08/2014, 14:21
... only the PTH11 domain was used to build the tree (the amino-terminal CFEM domain seen in a few proteins was not included), the 13 CFEM domain-containing proteins occurred together in one clade, indicating ... other proteins that were retrieved in the BLAST search, including mPR receptors (mPR_dom, Figure 2d) The proteins containing the PFAM GPCR domains are indicated in Table It is worth noting that the ... that mark the boundaries of these domains are given The location of the domains on the membrane topology shown for the M grisea protein is the same for other proteins that share these domains For...
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Báo cáo y học: "Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors" pot
Ngày tải lên :
14/08/2014, 17:22
... 82 proteins involved in these processes (only 26 were described previously) These proteins may play crucial roles in maintaining the eye in a healthy condition Perturbation of these proteins in ... statistical reasons On the other hand, the most abundant proteins in the sample had better sequence coverage in the Orbitrap data than in the The 491 proteins identified in the in- gel analysis were ... approximately one-third of the proteins identified in the LTQ-FT analysis were not detected on the Orbitrap (112 of 320 proteins) Interestingly, most of the proteins that were exclusive to the LTQ-FT analysis...
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Báo cáo y học: "Insights into the regulation of intrinsically disordered proteins in the human proteome by analyzing sequence and gene expression data" potx
Ngày tải lên :
14/08/2014, 21:20
... to the amino group of lysines of target proteins Diverse forms of ubiquitin modifications exist and influence the functional outcome of target proteins in distinct ways [52,53] Mono-ubiquitination ... suggests that there is a reduction in the catabolism of biopolymers (such as proteins) and that the transcripts encoding highly disordered proteins and the resulting protein product remain in the cell ... ubiquitination sites against the percentage of disorder (secondary y -axis, line plots) The transcripts encoding the disordered proteins, the ordered proteins and the proteome are shown in burgundy, mauve...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
Ngày tải lên :
13/11/2014, 09:25
... during the cycles of freezing and thawing, which leads to the exposure of new Ca++-binding sites Binding of Ca++ to these sites caused inhibition of the antifreeze and chitinase activities of these ... II Chitinases Class I and II chitinases belong to the PR-3 family of PR proteins Class I chitinases have a cysteine-rich chitin-binding domain (CBD) at the N-terminus The CBD is linked to the catalytic ... freezing and thawing, which led to the exposure of new Ca++-binding sites Binding of Ca++ to these sites caused inhibition of the antifreeze and chitinase activities of these complexes 1.3 The...
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Sterol rich membrane domains and membrane associated proteins in the fission yeast schizosaccharomyces pombe
Ngày tải lên :
15/09/2015, 17:09
... the actomyosin ring is pushed by the ongoing deposition of septum material in the plasma membrane invaginations following the constricting ring However, in both cases one would expect a linking ... targeting to the middle of the cell (Fig 3, 60 to 90 min) Filipin also stained the plasma membrane invaginations that follow the constricting ring (Fig 3, 105 min) After constriction of the ring to ... staining as observed during cytokinesis (Fig 4D) regardless of the presence or absence of an actomyosin ring (data not shown) However, the majority of cells exhibited a faint filipin staining in...
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The role of TRIP br proteins in the regulation of mammalian gene transcription and cell cycle progression
Ngày tải lên :
16/09/2015, 17:12
... Features PHD zinc finger protein PHD zinc finger protein Composite PHD zinc fingerbromodomain proteins PHD zinc finger protein Bromodomain protein PHD zinc finger proteins PHD zinc finger protein Composite ... Prominent examples of PHD zinc finger and/or bromodomaincontaining proteins The (*) label designates disease-associated PHD zinc finger and/or bromodomain-containing proteins Conservation of the ... So far, the physiologic roles of the TRIP-Br proteins remain elusive Nevertheless, there are several lines of indirect but compelling evidence inferring a role for the TRIP-Br proteins in regulation...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... Protein targeting to the microsomes requires the cotranslational insertion of the newly synthesized protein into the microsomal membrane, where the N-terminal hydrophobic signal sequence of the ... mitochondria-targeting signal at the 33–44 positions of CYP1A1 [4,8,9] This endoprotease was unable to cleave the N-termini of CYP2E1 and CYP2B1 [6,12] In the case of CYP2B1 and CYP2E1, uncleaved intact proteins ... localized inside of the inner membrane Moreover, the imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins are localized in the...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
Ngày tải lên :
19/02/2014, 16:20
... immunoblotting data, it was of interest to re-examine the fate of calsequestrin by studying the entire complement of key Ca2+ -binding elements in dystrophindeficient skeletal muscle fibres Because the ... that eight of these species are greatly reduced in mdx preparations This clearly indicates a drastic effect of the deficiency in dystrophin on the expression of Ca2+ -binding proteins The relatively ... combination of the pI value and molecular mass of individual 2D spots can be useful in the initial identification of proteins However, owing to the abnormal electrophoretic mobility of certain proteins, ...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
Ngày tải lên :
07/03/2014, 00:20
... shown in Fig 1B, the distance between the two termini of the inserted Ca2+ -binding sites in the model structure of the EF-hand of STIM1 is within ˚ 15 A Accordingly, a total of six glycine linkers ... substitutions at Ca2+ -coordinating loop positions and was introduced to perturb the Ca2+ -binding ability of the grafted EF-hand of STIM1 (B) Modelled structure of the engineered protein with the grafted ... is challenging to differentiate which region contributes to the oligomerization process To overcome the limitations of investigating the Ca2+ -binding sites in native Ca2+ -binding proteins, we established...
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Báo cáo Y học: The presence of a helix breaker in the hydrophobic core of signal sequences of secretory proteins prevents recognition by the signal-recognition particle in Escherichia coli doc
Ngày tải lên :
08/03/2014, 09:20
... exposing the signal-anchor domain just outside the ribosome To compensate for the loss of methionines from the deleted domains of the proteins, both constructs contain a C-terminal tetra-methionine ... hydrophobicity of the targeting signal is the parameter discriminating between SRP-dependent and SRP-independent pathways [14] On the other hand, in vitro cross-linking studies have revealed that the binding ... substrates for the SRP pathway To determine whether components of the SRP pathway are indeed involved in the targeting of (G-10L)prePhoE to the translocon, in vitro cross-linking studies were...
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Báo cáo khoa học: Exogenous oxidative stress induces Ca2+ release in the yeast Saccharomyces cerevisiae potx
Ngày tải lên :
15/03/2014, 23:20
... responsible for release of Ca2+ from the vacuole to the cytosol The YVC1 gene deletion drastically reduced the amplitude of the Ca2+ burst, indicating that most of the Ca2+ released into the cytosol after ... by the addition of BAPTA (Fig 2H), suggesting that the small Ca2+ burst seen in yvc1D cells was the result of Ca2+ in ux from outside the cell As BAPTA addition removes 50% of the signal in the ... cytosolic Ca2+ elevation was rather modest in the yvc1D mutant The residual Ca2+ increase observed in the yvc1D mutant could be explained by the existence of a low-affinity Ca2+ system of unknown...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... number of microbes present in the inoculum Afterwards, to examine the killing activity of Esc(1–18) against E coli and to determine whether this process was affected by the ionic strength of the incubation ... (p0), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the subphase was monitored for the ... (Fig 10) The exposure of · 107 cellsÆmL)1 at the corresponding MIC of Esc(1–18) resulted in an irregular rod form with a deep wrinkling of the cell surface (within min) However, all of these changes...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
Ngày tải lên :
16/03/2014, 13:20
... referring to all of them as caveolae, was demonstrated by their content of both caveolin-1 and caveolin-2 The coexistence of caveolin-1 and caveolin-2 in all three caveolae subclasses is in line ... increased in response to insulin in both the LD-caveolae and HD-caveolae, with the increase being particularly pronounced in the LD-caveolae (Fig 4C) The presence of the triacylglycerol-hydrolysing ... Also, the ratio of caveolin to cholesterol varied widely, being about six times lower in the LD-caveolae than in the closed VHD-caveolae, demonstrating that factors other than caveolin control the...
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