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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf

Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf

Báo cáo khoa học

... region was constructed by ampli-fying the whole plasmid pEU-scFvLH by inverse PCRwith the primers s2: 5¢-CAAAAAATTGAATGGCATGAACCGCCGAGCTCCAAC-3¢ and a2 : 5¢-AGCTTCAAAAATATCATTTAAACCCGACGGGCTGCTTTT-3¢(the ... disulfide-containing protein through a batchcell -free system from wheat germTakayasu Kawasaki1, Mudeppa D. Gouda1, Tatsuya Sawasaki1,2, Kazuyuki Takai1,2and Yaeta Endo1,21Cell -free Science and ... was amplified on the pEU–scFvLH using SP6primer (5¢-ATTTAGGTGACACTATAG-3¢)andanti-pri-mer (5¢-ATGGCGCCAGCTGCAGGCTA-3¢, anti-stopcodon in bold), and transcribed in the same way as above.Translation...
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Building wordpress themes from scratch

Building wordpress themes from scratch

Thiết kế - Đồ họa - Flash

... 'Primary Sidebar', 'primary-sidebar' ), 'id' => 'primary-widget-area', 'description' => __( 'The primary widget area', 'dir' ... it is automatically loaded during WordPress initialization (both for admin pages and external pages). ”13You can add features like sidebars, navigation menus, thumb-nail support, and more. ... page as a template in the WordPress editor:This template will supersede any other page-specic template that can be applied to the page. For example, if our page ID is 13, and we have a page-13.php...
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OReilly.Building.a.Web.2.0.Portal.with.ASP.NET.3.5.Jan.2008-BBL

OReilly.Building.a.Web.2.0.Portal.with.ASP.NET.3.5.Jan.2008-BBL

Kỹ thuật lập trình

... can add more widgets from a widget catalog and decorate the page as they like.How an Ajax-Powered Start Page Is DifferentThe advantages of Ajax and a rich client-side experience give users a ... any part of the Start page asynchronously and giveany web site an Ajax look-and-feel. However,UpdatePanels are a significant drag onthe page. The moreUpdatePanels you have, the slower asynchronous ... the SharePoint Portal, then you already know about widgets, which are calledWeb Parts in SharePoint and ASP.NET 2.0.Specifically, an Ajax-powered web portal is a web portal that uses Ajax technologiesto...
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Building a Sample Application Using ASP.NET AJAX

Building a Sample Application Using ASP.NET AJAX

Kỹ thuật lập trình

... 10:47 AM Page 239226 CHAPTER 10 ■ BUILDING A SAMPLE APPLICATION USING ASP.NET AJAXFigure 10-1. An ASP.NET AJAX-based stock applicationUnderstanding the Application ArchitectureThe application ... have added AJAX capabilities to this application, andthe page will not blink as it obtains data and renders the updates.You can see that there is no explicit coding for a partial-page update ... youlooked at a real-world application and how you would implement it as an AJAX applica-tion quickly, simply, and powerfully using ASP.NET AJAX.CHAPTER 10 ■ BUILDING A SAMPLE APPLICATION USING ASP.NET...
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LINUX FROM SCRATCH

LINUX FROM SCRATCH

Hệ điều hành

... 103Installing Man 104Installation of Man 104Contents 104Description 104man 104Linux From Scratch viiiTable of ContentsLinux From Scratch 1Gerard Beekmans 1Dedication 2Preface 10Who ... want to read this bookUsers who don't want to build an entire Linux system from scratch probably don't want to read this book. Ifyou, however, do want to learn more about what happens ... there is a lot of duplicate information in certain parts,it's the easiest way to read it and not to mention the easiest way for me to maintain the book.Part I − IntroductionPart One gives...
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The Practical Guidelines for Building a Business Plan in Five Pages

The Practical Guidelines for Building a Business Plan in Five Pages

Anh văn thương mại

... anticipated that catches management unprepared. A contingency plan should address such occurrences. Natural disasters are a primary contingency that companiesplan for. Like manmade situations, these ... viable method to increase the skill levels of your man-agers and employees goes a long way to creating a success from theplan. Building your education and training as an adjunct to the plan-ning ... brief-ing. Make sure participants understand that your planning model isabout to take a dramatic turn for the better. Business-as-usual can-not be allowed. Building a Business Plan in Five Pages39Figure...
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Lab 5.1.11 Building a Peer-to-Peer Network

Lab 5.1.11 Building a Peer-to-Peer Network

Quản trị mạng

... Layer 3, so that they can communicate. This lab does not require a hub or any other interconnecting network device. A basic CAT 5/5e UTP crossover cable is all that is needed. A crossover cable ... accomplished using a cat 5 or 5e crossover cable. Locate a cable that is long enough to reach from one PC to the other, and attach one end to the 3 - 4 CCNA 1: Networking Basics v 3.0 - Lab 5.1.11 Copyright ... directly connected. The default Gateway is only required on local area networks that are connected to a router. Computer IP Address Subnet mask Default Gateway PC – A 192.168.1.1 255.255.255.0...
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Lab 5.1.12 Building a Peer-to-Peer Network

Lab 5.1.12 Building a Peer-to-Peer Network

Quản trị mạng

... directly connected. The default gateway is only required on local area networks that are connected to a router. Computer IP Address Subnet mask Default Gateway PC – A 192.168.1.1 255.255.255.0 ... two PCs a. Set the IP address information for each PC according to the information in the table. b. Note that the default gateway IP address is not required, since these computers are directly ... restored at the end of the lab. These include IP address, subnet mask, default gateway, and DNS servers. If the workstation is a DHCP client, it is not necessary to record this information. ...
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Lab 5.1.13a Building a Hub-based Network

Lab 5.1.13a Building a Hub-based Network

Quản trị mạng

... accomplished using a Category 5 or 5e straight-through patch cable. Locate two cables that are long enough to reach from each PC to the hub. Attach one end to the NIC and the other end to a port on ... to as a multiport repeater. Hubs are inexpensive and easy to install, but they permit collisions to occur. They are appropriate for a small LAN with light traffic. In addition to the physical ... ping command Background / Preparation This lab focuses on the ability to connect two PCs to create a simple hub-based Ethernet LAN using two workstations. A hub is a networking concentration...
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Lab 5.1.13b Building a Switch-based Network

Lab 5.1.13b Building a Switch-based Network

Quản trị mạng

... the two PCs and the switch will be accomplished using a Category 5 or 5e straight-through patch cable. Locate two cables that are long enough to reach from each PC to the switch. Attach one end ... Layer 3, so that they can communicate. Since this lab uses a switch, a basic Category 5/5e UTP straight-through cable is needed to connect each PC to the switch. This is referred to as a patch ... dedicated bandwidth to workstations. Switches eliminate collisions by creating microsegments between ports to which the two workstations are attached. They are appropriate for small to large LANs...
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Building A Windows Live CD

Building A Windows Live CD

Hệ điều hành

... from a deleted partition. The steps required to add any program will be the same as the ones we will use to add the data-recovery tool. Also, you can have any wallpaper that you want-be ...    Windows XP LIVE CD    Any PC user can create a CD that runs Win XP without being installed, and use it to troubleshoot and recover crashed machines.   Posted by: blackram@projectallabout  Edited by: CairaC    9 CHANGE ... [PEBuilder] Name=”Data Recovery Tool” Enable=1 [WinntDirectories] a= ”Programs\ datarecovery “, 2 [SourceDisksFiles] abc.exe =a, ,1 xyz.dll =a, ,1 [Software.AddReg] ;Add to Start Menu\Programs 0x2,”Sherpya\XPEinit\Programs”,”DataRecovery...
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