brdu incorporated skeletal myoblasts were grown on a glass chamber 1 x 105 per glass chamber the detection of brdu was carried out using brdu label

Studies on the anti cancer potential of sesquiterpene lactone parthenolide

Ngày tải lên : 16/09/2015, 17:14

... stimuli Caspase Bax P Bid APOPTOSIS Bad Bc l-2 Bak a tBid Bak Bak id tB ax Bax a Ba x tBid Bc P Bad l -X Downstream caspase Apaf -1 L Caspase cyto c smac Fig 1. 7 Mitochondria and Bcl-2 family: the central ... et al., 19 99) On the other hand, the activation of caspase and can be negatively 29 regulated by binding of IAPs such as cIAP1/2, XIAP and survivin (Deveraux and Reed, 19 99) The final caspase activity ... apoptosis cascade There are two classical pathways that lead to activation of caspase: the death receptor pathway and mitochondrial pathway The death receptor pathway starts from a group of death receptors...

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Human telomerase reverse transcriptase (hTERT) overexpression modulates intracellular redox balance and protects cancer cells from apoptotic cell death

Ngày tải lên : 14/09/2015, 08:38

... oxide radical (NO.), hydroxyl radical (OH.) and the peroxyl radical and the non radicals hydrogen peroxide 13 (H2O2) and peroxynitrite (ONOO-) They are generated when the unpaired electrons of oxygen ... that an increase in intracellular H2O2 can lead to cytosolic acidification which creates a permissive intracellular milieu for protease activation and/or Bax conformational change and mitochondrial ... et al., 19 99) On the other hand the vast majority of human tumours seem to depend on the reactivation of the telomerase protein to confer cellular immortality These conditions that arise from the...

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Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

Ngày tải lên : 18/02/2014, 13:20

... oral bioavailability and active epithelial excretion of paclitaxel (Taxol) caused by P-glycoprotein in the intestine Proc Natl Acad Sci USA 94, 20 31 2035 Kamimoto Y, Gatmaitan Z, Hsu J & Arias ... determination of the amount of dyes associated with them The advantages of such an assay are that: (a) it is quantitative; (b) it is not affected by intracellular quenching of the dyes; and (c) ... assumption is that the afﬁnity of rhodamines to intracellular membranes is not very different from their afﬁnity towards the extracellular surface of the plasma membrane Because the surface area of...

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Tài liệu World Economic outlook - Financial Stress, Downturns and Recoveries ppt

Ngày tải lên : 18/02/2014, 16:20

... Equatorial Guinea, Ethiopia, Gabon, Ghana, Guinea, Kenya, Madagascar, Mali, Mauritius, Mozambique, Namibia, Niger, Nigeria, Rwanda, Senegal, South Africa, Sudan, Tanzania, Uganda, and Zambia 5Bahrain, ... Initial Conditions 17 9 5 .10 Effect of Fiscal Expansion in a Large Economy 18 2 vii contents 5 .11 Fiscal Expansion in a Large Economy Compared with a Small Open Economy with Monetary Accommodation ... inflation excluding food and fuel has also accelerated markedly, and there are signs of rising inflation expectations and wage increases, although such data are not as systematically available as...

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Tài liệu Báo cáo khoa học: Osmotic stress sensing and signaling in ﬁshes doc

Ngày tải lên : 18/02/2014, 16:20

... Ionic strength and the polyvalent cation receptor of shark rectal gland and 13 14 15 16 17 18 19 20 21 22 23 24 25 artery J Exp Zool Part A Comp Exp Biol 30 1A, 235– 239 Flanagan JA, Bendell LA, ... (8 71 amino acids, AAG28029 .1) , aquaporin (323 amino acids, NP_0 016 41. 1), TonEBP (15 31 amino acids, NP_006590 .1) , and the mouse sequence of TSC22D2-4 (11 6 amino acids, EU00 415 1) were used as references ... the millimolar range over which CaSR operates [11 ] In the mammalian kidney, CaSR regulates the activity of many other signaling pathways, including pathways that are regulated by intracellular...

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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Ngày tải lên : 21/02/2014, 01:21

... Downstream primer (5¢-to 3¢) Annealing temp (°C) Seg size (bp) TGA AGC TGG AAA CCA TCA TTC AAA ACA TTA GCA GGA ATT TTC 52 343 GGA GTT CTG CCA GGG AAC CAC GTG CTG CCT GAG AAG GAT TG GAC AGG GGA GAA ... ATRA or TPA was added at the same concentrations as above The cells were incubated for an additional 24-h period Following the second incubation the cells were counted and separated from the ... staining was performed on cells cultured for days Cystatin F was stained as described above and after the last washing step 20 lgÆmL )1 Texas Red conjugated concanavalin A (Molecular Probes) was...

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Stress symptoms and coping strategies in healthy elderly subjects pot

Ngày tải lên : 14/03/2014, 20:20

... emotional and situational control strategies than confrontive actions (15 ) Furthermore, literature data appoint emotional control, achieved on the basis of emotion-focused coping, as a favorable and ... like personality trait, as well as social and family support, can additionally interfere in the adaptation and control of a given stressful situation(6,9) Regarding the stress manifestation, physical ... regroup the eight different coping styles and classify them on the basis of the characterization of the coping action focus (emotion and problem) This new classification was based on the definitions...

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Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx

Ngày tải lên : 16/03/2014, 13:20

... nm The monitoring light was produced using a 15 0 W xenon lamp (Hamamatsu Photonics, Hamamatsu, Japan) The peak power of the laser was 10 mJ with a pulse width of ns (a repetition rate of 10 Hz) ... FEBS 12 15 Heme electronic states of Leu mutants of Ec DOS N Yokota et al (Table 3) In addition, the rate of auto-oxidation for L99F was fast but comparable to that of the L 115 F mutant (Table 2) The ... La Jolla, CA, USA) with pET2 8a( +)-wild-type Ec DosH as a template and using the following respective 5¢-sense primers: 5¢-gatga gtcgggagACCcagctggagaaaaaag-3¢, 5¢-gatgagtcgggagTTTcag ctggagaaaaaag-3¢,...

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Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt

Ngày tải lên : 18/03/2014, 01:20

... experiments are: Oligo I, 5¢-CAAGGACGTTCGATGCA CTTCCAAAAAACATATAAT-3¢; Oligo II, 5¢-CAAT GTAGTATTAAAAAGTAGTAGTTAAAAGC-3¢; Oligo III, 5¢-GTTTTTATCCGATGCAAATTTTTGCTTTGT GATTG-3¢ The reaction was ... 5¢-GTTGGGAT GAACCAGAAGGA-3¢; reverse primer: 5¢-GAACCA CCGATCCAGACACT-3¢) as a control Reactions with no DNA added served as a negative control The PCR cycling proﬁle was: denaturation at 92 °C ... Hybridization was carried out in the presence of 50 lgÆmL )1 sonicated calf thymus DNA and labeled probe at room temperature for h The membrane was washed, dried and exposed for autoradiography RESULTS...

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Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx

Ngày tải lên : 29/03/2014, 21:20

... (Fig 1B) Ultrastructural examination revealed that relaxin-3 was localized to the dense-core vesicles in the perikarya, and it was also observed in the synaptic terminals of axons [12 ] The NI ... the periaqueductal gray matter, and the area dorsal to the substantia nigra in the midbrain reticular formation By immunostaining using monoclonal antibody against the N-terminus of the human ... in the activation of relaxin-3 gene transcription Regulation of relaxin-3 gene expression Concerning the regulation of relaxin-3 gene expression, relaxin-3 mRNA expression in the NI is enhanced...

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oxidative stress biomarkers and antioxidant protocols

Ngày tải lên : 11/04/2014, 10:00

... Dean 10 1 12 Rapid Determination of Glutamate Using HPLC Technology Aqeela Afzal, Mohammed Afzal, Andrew Jones, and Donald Armstrong 11 1 13 A Rapid Method for the Quantification of ... (19 86) Quantitation of lipid peroxidation products by gas chromatography-mass spectrometry Anal Biochem 15 2, 10 7 11 2 11 VanRollins, M and Murphy, R C (19 84) Autoxidation of docosahexaenoic acid: ... phosphatidylethanolamine and from products of lipid oxidation (4,9 ,11 ) The aldehyde bands sometime appear near 16 00 cm 1 (3), depending upon the salt of the carboxylic acid To measure the area of these bands,...

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báo cáo hóa học: " Occurrence of post traumatic stress symptoms and their relationship to professional quality of life (ProQoL) in nursing staff at a forensic psychiatric security unit: a cross-sectional study" potx

Ngày tải lên : 18/06/2014, 18:20

... (47 .1% ) had >12 years of nursing experience in psychiatry, 24 (34.2%) had 4 12 years of experience and only 13 (18 .6%) had less than years of experience Among the respondents, the most experienced ... if one of the treatment principles, i.e making the patient treatable at a lower security level can explain the low compassion satisfaction scores at ward A, the admission ward At this particular ... formal approval as only staff members participated and the study was part of the hospitals internal actions to initiate Page of (page number not for citation purposes) Health and Quality of Life Outcomes...

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báo cáo hóa học: " Prevalence of stress, anxiety and depression in with Alzheimer caregivers" ppt

Ngày tải lên : 18/06/2014, 19:20

... unable to accept the situation and wish to abandon their home Mean values of MMSE, ADL and IADL of the patients and CBI of the caregivers are outlined in Table At the same time as the survey on ... complexity of the treatment, the constant commitment of the person taking care of the AD patient, as well as the inadequacy of the public service; the consequent effects upon the emotional and ... survey on the caregivers, an assessment was made of the severity of the patients' pathological conditions: linear regression demonstrated that an increase in the degree of anxiety and depression is...

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báo cáo hóa học:" Psychosocial stress, demoralization and the consumption of tobacco, alcohol and medical drugs by veterinarians" ppt

Ngày tải lên : 20/06/2014, 00:20

... and the association between onset of use and alcohol-related problems in a general population sample in Germany In this study 75 01 persons were asked about their alcohol consumption This was based ... hours and demoralization Tobacco consumption 19 .4% of veterinarians are smokers The 2003 Telephone Health Survey, based on a randomly generated sample of 8 318 persons in the resident German-speaking ... Gender Male Female 30.3 33.3 1. 2 (0.9 1. 5) 1. 3 (1. 0 1. 7) Professional work Non-clinical area of work1 Practice Owner Employee in a Practice 29 .1 36.5 25 .1 1.4 (1. 0 1. 9) 0.8 (0.6 1. 2) 1. 4 (1. 1–2.0)...

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báo cáo hóa học: " Quality of life in Croatian Homeland war (19911995) veterans who suffer from post-traumatic stress disorder and chronic pain" doc

Ngày tải lên : 20/06/2014, 15:20

... disease with a lifelong course [11 ] One of the main disadvantages of this disease is related to the existence of chronic pain, which may be affecting as much as 80% of the PTSD population [12 ,13 ] ... PTSD Braš et al Health and Quality of Life Outcomes 2 011 , 9:56 http://www.hqlo.com/content/9 /1/ 56 Page of Table The comparison of quality of life among study groups using analysis of variance and ... remains a substantial problem in Croatian health care, with large number of reported cases and high overall burden for both health system and society in total Page of 8 10 11 12 13 14 15 16 17 18 ...

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Stress, Strain, and Structural Dynamics ppt

Ngày tải lên : 29/06/2014, 09:20

... Introduction 11 .2.2 Modes of Vibration 11 .2.3 Solution by the Toolbox 11 .3 Dynamic Response 11 .3 .1 Modal Analysis 11 .3.2 Laplace Transform Method 11 .3.3 Plotting Analytical Solutions 11 .3.4 Runge-Kutta Algorithm ... 10 .4 Analytical Vibration Solutions 10 .4 .1 Analytical Methods 10 .4.2 Solution by the Toolbox 10 .5 Frequency Response 10 .5 .1 Harmonic Excitation 10 .5.2 Base Excitation 10 .5.3 Response under Rotating ... Runge-Kutta Algorithm 11 .3.5 Solution by Superposition 11 .3.6 Harmonic Excitation 11 .4 Dynamic Vibration Absorption 11 .4 .1 Undamped Vibration Absorbers 11 .4.2 Damped Vibration Absorbers 437 437 439...

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Tài liệu tiếng anh tham khảo stress coping and health

Ngày tải lên : 09/07/2014, 08:25

... primary mediator of person–environment transactions was appraisal Three types of appraisal were identified: primary, secondary, and reappraisal Primary appraisal is a judgment about what the person ... both the initial impact of the stressor (alarm reaction) on tissues and the adaptive mechanisms that are a reaction to the stressor In addition, conceptual confusion about the meaning of the term ... an outgrowth of person and environmental interactions As long as a person is capable of evaluating how he or she is feeling and doing at some level, the person has health For example, an infant,...

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Devaluation and the trade balance

Ngày tải lên : 23/07/2014, 16:28

... from a decrease of 19 per cent of the quantity purchased abroad a t constant prices or from a decrease of only 10 per cent of the quantity purchased combined with a fall of foreign prices by 10 per ... as the condition for the ratio of exports to imSince (12 ), the condition for a favorable ports to increase upon devaluation effect of devaluation on the domestic balance, Our results permit the ... considered a s given, the effect of devaluation on the trade as the condition for the foreign balance to im- balance expressed in foreign exchange will be the more favorable, the greater the relative...

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Báo cáo sinh học: " Genome degeneration affects both extracellular and intracellular bacterial endosymbionts" pot

Ngày tải lên : 06/08/2014, 19:20

... they are extracellular or intracellular It appears that the decisive evolutionary constraint is the spatial isolation of the bacteria, either by intracellular confinement in bacteriocytes or, as ... Extracellular in midgut crypts Extracellular in midgut crypts Intracellular Facultative intracellular Extracellular Extracellular Vertical Vertical Horizontal Horizontal/ vertical Horizontal Transmission ... mutualists and pathogens is that in the beneficial bacteria genome degeneration preferentially tends to affect catabolic pathways, whereas in parasitic bacteria predominantly anabolic pathways are...

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Báo cáo sinh học: " Oxidative stress, thermogenesis and evolution of uncoupling proteins" doc

Ngày tải lên : 06/08/2014, 19:20

... termed BMCP1) as a sister group of plant UCPs and animal UCP1-3 The other members of the superfamily analyzed - PiC, ANT, OGC (oxoglutarate carrier) and DIC (dicarboxylate carrier) - were found ... potential members of the superfamily (NCBI conserved domain Pfam0 015 3, mito_carr superfamily) The elucidation of the three-dimensional structure of the ANT has confirmed this structural arrangement ... all other UCPs Even in the carp UCP1, position 13 4 is Asp, and the biochemical characterization of carp UCP1 revealed no nucleotidesensitive basal proton conductance [12 ] In addition, several groups...

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