bluefin tuna thunnus maccoyii implication for precise estimation of recruitment abundance indices
Fisheries science JSFS , tập 76, số 2, 2010 3
... thank Prof Masaaki Tanaka of the Faculty of Environmental and Information Sciences, Yokkaichi University, for his advice regarding the identification of the phytoplankton, Dr Mark J Grygier of Lake ... for the growth of a wide range of species, indicating that only a few species of facultatively anaerobic bacteria may inhabit the whale intestine E faecalis is often isolated from the feces of ... source of mortality Such a potential linkage of growth to survival led to the assumption that the detection of strong selection for fast growth during early life of a given cohort is a symptom of...
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BÁO CÁO " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu s vannamei) " pptx
... effect of temperature and salinity on osmoregulation of juvenile and subadult Penaeus stylirostris Aquaculture 209:307-317 Lignon J.M 1987a Ionic permeability of isolated gill cuticle of shore ... Castille Jr F.L and A.L Lawrence 1981a The effect of salinity on the osmotic; sodium, and chloride concentrations in hemolymph of euryhaline shrimp of genus Penaeus Comp Biochem Physiol 68A:75-80 ... THAM KHẢO Avenet P and J.M Lignon JM 1985 Ion permeabilities of gill lamina cuticle of the crayfish Astacus leptodactylus (E.) Journal of Physiology 363:377-401 Cameron J.N and P Thomas 1992 Calcitonin-like...
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Báo cáo nghiên cứu khoa học: " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu svannamei)" ppsx
... effect of temperature and salinity on osmoregulation of juvenile and subadult Penaeus stylirostris Aquaculture 209:307-317 Lignon J.M 1987a Ionic permeability of isolated gill cuticle of shore ... Castille Jr F.L and A.L Lawrence 1981a The effect of salinity on the osmotic; sodium, and chloride concentrations in hemolymph of euryhaline shrimp of genus Penaeus Comp Biochem Physiol 68A:75-80 ... THAM KHẢO Avenet P and J.M Lignon JM 1985 Ion permeabilities of gill lamina cuticle of the crayfish Astacus leptodactylus (E.) Journal of Physiology 363:377-401 Cameron J.N and P Thomas 1992 Calcitonin-like...
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Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot
... complexes by forming nucleosomes, when their synthesis ceases at the end of S-phase This would explain the end of the activity period of DNA polymerases Instead of assuming a neutralization of the ... measured in the presence of added mM spermidineÆ3HCl (?) to neutralize the inhibitory activity of PMLA In the absence of spermidineÆ3HCl, activities of DNA polymerase, except of DNA polymerase b-like, ... During continued addition of the polymerase, the enzyme activity increased in parallel with the activity of the control experiment in the absence of extract An amount of 0.4 U of the reporter DNA polymerase,...
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effects of magnesiumphosphoruspotassium containing in water on molting, growth and survival rate of the white shrimp (litopenaeus vannamei) juveniles, reared in low salinity water
... iii TABLE OF CONTENTS iv LIST OF TABLES vii LIST OF FIGURES viii LIST OF FIGURES viii LIST OF ABBREVIATIONS .ix LIST OF ABBREVIATIONS ... 1999) K+ Weekly AAS (APHA et al., 1999) Growth indices performance Survival rate (%) End of the experiment End of the experiment 11 Shrimp performance indices are measured: - Weight gain, WG: WG ... mean initial total number of shrimp; Nf: mean of total number of shrimp at the end of experiments 3.3.2 Experiment 2: Effects of Mg-P-K supplemented on molting intervals of white shrimp L vannamei...
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Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx
... recognition of pNPP by the pNPP site at the rear surface of the N-domain is due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398 The hydrophobicity of the binding ... presence of any of these H4-H5 loopGST ể FEBS 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3929 Fig Recognition of FITC by amino acids forming the ATP binding site of ... pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+/K+ ATPase and H+/K+ ATPase in the range of the binding site for pNPP Bold, amino acids interacting with...
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Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
... Ashcroft, at the Diabetes Research Laboratories, University of Oxford for the use of his luminometer; and to Dr Simon Golding at the Department of Physiology, University of Oxford for the use of ... by impairment of the Ó FEBS 2003 ATP regeneration system, nor by loss of ATP or by changes in concentrations of Na+ and K+ levels These experiments were performed in the absence of a-crystallin, ... cytosolic side of the ghost cell The means by which it affords such protection is unknown but presumably inactivation of the enzyme by the modifier is due to the targeting of a domain of the enzyme...
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Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
... amount of a : (v/v) mixture of methanol and chloroform in a test tube and the solvent was then evaporated with nitrogen gas ¯ushing The ®lm of soy-PtdCho which formed on the inner wall of the ... subunit dissociation, conformational change of protein, and/or the destruction of integral structure (lipid bilayer and protein) The issue of clarifying the precise nature of the interrelationship ... of transmembrane a helices, and a separation in the contact surface of membrane and protein due to the thickening and shrinkage of lipid bilayer For the last case, a quantitative estimation of...
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Na+, k+ ATPase in single skeletal muscle fibres and the effects of ageing, training and inactivity
... isoforms (α1-3, β1-3) of the NKA are expressed The basic function of each isoform is listed below in Table 1.1 Table 1.1 Basic functions of the NKA isoforms in skeletal muscle NKA Function isoform ... of each isoform, as a knockout of either of the three α isoforms leads to the death of rats and mice before or immediately following birth (Lingrel et al., 2007) Research using isoform knockout ... identify which of the α isoforms may have been detected and furthermore no information can be obtained regarding abundance of the beta isoforms or FXYD1 37 The use of rat skeletal muscle for quantifying...
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Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx
... structure of the cytoplasmic domain, i.e an open form and a compact form The Ca2–E1 state is an open form [45–47] Binding of the nucleotide converts it into a compact form [47] The compact form induced ... presence of NaCl at concentrations of more than mm (Fig 1B) In the presence of 0.3 mm KCl, the inhibitory effect of Na+ was absent in the absence of oligomycin (Fig 1C) The addition of oligomycin ... different from that of the Ca2– nucleotide–E1 state [47] The crystal structure of Ca2+-ATPase is suggested to be similar to that of Na+ ⁄ K+-ATPase [48–50] Therefore, the conformations of the intermediates...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... re-replication of DNA in a single cell cycle Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7] Targets of the kinase in the regulation of DNA replication ... the retarded mobility of the protein in SDS gel, compared with the mobility of protein pre1226 Fig Specificity of binding of phosphoMCM4 antibodies The binding specificity of six antiphosphoMCM4 ... question of whether CDK2 is responsible for the phosphorylation of MCM4 at CDK sites during interphase, a dominant-negative mutant of CDK2 [16] was expressed in HeLa cells, and the effect of its...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... phases of the cell cycle [0 h (S), 10 h (G2 ⁄ M) and 20 h (G1)] after synchronized cells were released at the S phase In the case of HoxA5, recruitment of RNAP II and MLL1, and the level of H3K4 ... these marks were attenuated for the rest of the cell cycle (10 and 20 h), correlating with the expression of the gene (Fig 6C,D) The correlation of promoter occupancy of MLL1, H3K4 trimethylation ... observed during mitosis However, the recruitment of MLL1 and the level of H3K4 trimethylation are modulated in the promoters of specific Hox genes as a function of their expression Importantly, as...
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Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx
... and -insensitive a-isoforms of Na+/K+-ATPase in rats All isoforms of the catalytic a-peptide of Na+/K+-ATPase have similar, high affinity for ouabain [17] with Kd in the order of one or a few nanomolar ... cascade of events initiated by ouabain occupancy of a small fraction of the sodium pumps One of them had advocated the role of EDLF for years [15] Possible pitfalls for a novel role of cardiac ... (35 kDa for the protein) with chaperone-like function Four isoforms of the a-peptide have been described (one in testes) In most mammalian species, Na+/K+-ATPase, irrespective of isoform composition,...
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Báo cáo Y học: Na+/K+-ATPase as a signal transducer Zijian Xie and Amir Askari docx
... the transactivation of the EGF receptor by Src is sufficient to account for the recruitments of Shc (SH-2 domain-containing protein), Grb2, Sos (a mammalian homologue of Son -of- sevenless), and ... the existence of another positive feed-back cycle; i.e the requirement of rise in [Ca2+]i for MAPK activation, and the necessity of MAPK activation for rise in [Ca2+]i Two pools of Na+/K+-ATPase ... activation of this cascade, unlike that of ROS generation, also requires the rise in [Ca2+]i that is caused by ouabain’s inhibition of the ion transporting function of Na+/K+-ATPase [25] One locus of...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
... others [28] In the history of the study of the growth of E coli there is one result that should be noted, that of Hoffman and Frank [29] who performed early time-lapse studies of bacterial growth They ... curve of radioactivity per cell indicates exponential growth The uptake of molecules is exponential for precursors of protein [23], stepwise for precursors of DNA [3,27,28,3038], exponential for ... be a precise measure of cell mass, as one must also assume that the diameter is constant For the sake of clarity of argument, it is accepted here that cell length of S pombe is a measure of cell...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... [30] For analysis of kinetics of apopto¨ sis, 3T6 cells (70% confluence) were infected the virus at a multiplicity of infection of plaque-forming unit per cell Viral adsorption was carried out for ... levels of apoptotic markers during the late stages of MPyV infection However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt MPyV, and replication of ... The plasmid for production of VP2 was prepared by insertion of amplified sequences into the HindIII and BglII sites, and the plasmid for production of VP3 was prepared by insertion of amplified...
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Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc
... for RNA 2614 GAT TGT AA GG CCC ATC TA GAT GGA GGG RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR RT-PCR Control Control for for for for for for for for expression of pri-miR9-1 expression of ... induction of P19 cells Experimental procedures Recombinant constructions of reporter gene to monitor primary and mature form of miR9 To detect the transcript level of pri-miR9, the upstream region of ... H Ko et al Bioimaging of miR9 and miR9* during neurogenesis higher endogenous level of mature miR9 than of mature miR9* In vivo visualization of unbalanced expressions of mature miR9 and miR9*...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... Therefore, binding of Mg2+ in a negatively cooperative fashion to the M3 site of dimeric APase increases the rate of the conformational change responsible for the activation of the enzyme Conformationally ... properties of APase from E coli The influence of Mg2+ on the kinetic properties of APase from E coli in the presence of a Zn2+ to dimer ratio of : in 2A2M1P buffer, (pH 10.5) at 25 °C in the absence of ... saturation of the subunits with Mg2+ could be the principal cause of conformational asymmetry of the homodimeric enzyme Reaction mixtures with and without 0.05 mM Pi, at a Zn2+ to dimer ratio of Fig...
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Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx
... analysis of the percentage of heat induced SK aggregation at pH 7.0 (A) Aliquots of SK were preincubated at 90 °C for 10 at different sample concentrations prior analysis (B) Aliquots of SK of 9.9 ... fluorescence spectrum of a 10 lM solution of ANS in the presence and absence of 10 lM of SKA1, at pH 7.0 and 4.4 The presence of the fragment produces a large increase in the intensity of ANS fluorescence, ... the absence of the SK fragment (B inset) Dependence with the pH of the wavelength of the maximum of the fluorescence spectra for the ANS + SK 1–63 mixtures relative to the spectrum of free ANS...
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Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... mass of 90 kDa and are typically homodimers of 45-kDa subunits [44,45] In the presence of Mg2+, glyoxysomal CS of plants also forms tetramers [43] However, there are also a number of bacteria for ... analysis of the evolution of these enzymes than we have performed here However, our aim was not to perform an exhaustive analysis but to obtain an overview of the patterns of similarity for the ... evolution of all of the enzymes of the pathway (Fig 4), it is clear that only about half of the enzymes of the tricarboxylic acid cycle, the major pathway of carbon metabolism in mitochondria of oxygen-respiring...
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