bioinformatic and functional optimization of antisense phosphorodiamidate morpholino oligomers pmos for therapeutic modulation of rna splicing in muscle

Bóa cáo y học: "Prospective, randomized trial comparing fluids and dobutamine optimization of oxygen delivery in high-risk surgical patients [ISRCTN42445141]" pot

Ngày tải lên : 12/08/2014, 23:23

... Effects of epinephrine, norepinephrine, or the combination of norepinephrine and dobutamine on gastric mucosa in septic shock Crit Care Med 1999, 27:893-900 Swisher SG, Holmes EC, Hunt KK, Gornbein ... marker of inflammation The dobutamine infusion was maintained for 24 hours in the postoperative period and then was slowly reduced until complete interruption The mechanical ventilation and weaning ... nosocomial infections, Centers for Disease Control definitions were used [20] Postoperative fistulas and dehiscence of anastomosis were determined by visualizing the elimination of intestinal content...

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CFD for hydrodynamic efficiency and design optimization of key elements of SHP

Ngày tải lên : 05/09/2013, 14:58

... publications being book in Small Hydro, 52 in Journals with referee and 110 in International Conferences; Supervisor of several post-doc, PhDs and MSc students and author of innovative real solutions in ... resulting in the Laplace equation, verifying that streamlines and equipotential lines are mutually perpendicular originating a flow net of streamlines and equipotential lines When the stream lines ... along the AB and CD segments In Graph the inversion of the flow velocity in the middle of the draft tube enhance the inversion of the flow due to the influence of the rotational speed and the pressure...

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Cooling load and COP optimization of an irreversible Carnot refrigerator with spin-1/2 systems

Ngày tải lên : 05/09/2013, 15:28

... engineering, and marine nuclear reactor engineering He is a Professor in the Department of Power Engineering, Naval University of Engineering, P R China Professor Sun is the author or coauthor of ... Degree in 2007 in science from Peking University, P R China, and received his MS Degree in 2009 in power engineering and engineering thermophysics from the Naval University of Engineering, P R China ... BS Degrees in 1982 in Physics from the Wuhan University of Water Resources and Electricity Engineering, P R China and received his PhD Degrees in 1998 in power engineering and engineering thermophysics...

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Numerical simulation and optimization of CO2 sequestration in saline aquifers for enhanced storage capacity and secured sequestration

Ngày tải lên : 05/09/2013, 16:10

... conducting several benchmark studies [3, 4] In this paper, we consider the optimization of two engineering techniques to increase the sequestration efficiency and safety of GCS in saline aquifers In ... number of independent variables reduces to two The injection rates of CO2 and the injection rates of water are chosen as the final optimization design variables Optimization of WAG operation for ... efficiency of GCS by increasing the required injection pressure to maintain well injectivity, and most importantly, it can potentially jeopardize the integrity of the formation matrix Therefore, it...

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Tài liệu Physical health and functional ability of an elderly, population in Sri, Lanka doc

Ngày tải lên : 14/02/2014, 06:20

... the time of the interview was identified as an "informant" At each interview where an informant was present, assessment of the informant of the health status of the elderly person was obtained Results ... carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full) A younger member of the household ... (IADL)" and included ability to travel outside, go shopping, prepare own meals and handle money A limited number of tests of physical performance for assessing the functioning of upper and lower...

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in complex with ImmGP, indicating a role in transition state stabilization during catalysis [17] A series of mutations ... activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain ... Mutations of Asp13 7in human HPRT to Asn resulted in a 290-fold and 18-fold decrease in kcat values for nucleotide formation with guanine and hypoxanthine bases, respectively, indicating that Asp137...

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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... amino acids located within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and ... also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA for ... coding for cytokines and chemokines (IL-1, IL-2, IL-6, TNFa, GM-CSF, interferons b and c) contain regulatory elements in the 3¢ UTR that are important for the half life of cytokine mRNAs For...

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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

Ngày tải lên : 18/02/2014, 16:20

... tight main-chain and medium side-chain restraints Model building was carried out in o [29] and coot [30] In chain A, residues and 169–176 are missing; in chain B, residues 167–172 are missing; in ... in chain C, residues 1, and 169–174are missing; in chain D, residues 168–174 are missing; in chain F, residues and 168–175 are missing All amino acid residues were present in the E chain, and part ... usually found in nucleotide binding enzymes is not present in UpUMPK [19] UpUMPK contains instead a glycine-rich motif within amino acids 44–54 that is responsible for binding of the phosphate...

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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

Ngày tải lên : 18/02/2014, 18:20

... protein Similarly, both the R186 residue in the Rep245 domain and K300, its counterpart in Pfu-primase, were contained in a loop that is plausibly involved in DNA recognition and binding and is ... stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then recording the CD spectra of the incubated samples at indicated temperatures Thermophilicity and thermostability ... a program for display and analysis of macromolecular structures Mol Graphics 14, 51–55 Supporting information The following supporting information is available: Fig S1 The in uence of (A) divalent...

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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

Ngày tải lên : 19/02/2014, 17:20

... DNA and RNA were performed as described previously [57] Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the ... Mancini JA (1992) Identiﬁcation of amino acid residues of 5-lipoxygenase-activating protein essential for the binding of leukotriene biosynthesis inhibitors Mol Pharmacol 42, 94–102 38 Mancini ... A major subgrouping is visible with MGST1, PGES and Insect in the upper part of the tree and the remaining families ⁄ groups in the lower part In the lower part, MGST2, FLAP and LTC4 synthase...

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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Ngày tải lên : 21/02/2014, 00:20

... obtained by simple rotation of the same model The flexible LID domain appears at top of the three figures, NMP-binding site and ATP binding domain are displayed, NH2- and COOH-termini are also indicated ... that of E coli AK The flexible LID domain which provides open and closed conformation to the protein during binding of the substrate is shown in the top-most portion of the protein ATP may bind ... the amino acid sequence of AK2 protein The purification and functional folding of L donovani AK from the E coli cell lysates was carried out by washing and dissolving the inclusion bodies in M...

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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identiﬁcation of a novel subtype of thyrotropin-releasing hormone receptor ppt

Ngày tải lên : 21/02/2014, 03:20

... consisting of 94 °C for 45 s, 45 °C for min, and 72 °C for min, followed by a ﬁnal extension at 72 °C for 10 Five microliters of this ampliﬁed mixture was then submitted to nested PCR using more internal ... domain protein of 401 amino acids (45.2 kDa) for xTRHR2 and 397 amino acids (45.0 kDa) for xTRHR1 Alignment of the deduced amino acid sequences with that of xTRHR3 (Fig 3) showed that xTRHR2 and ... especially xTRHR3 and xTRHR2 This is particularly interesting considering the functional importance of the third intracellular loop and the C-terminal tail in receptor coupling and regulation The...

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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Ngày tải lên : 21/02/2014, 15:20

... The cluster of basic residues in the C-terminal domain may constitute heparin binding-site residues that coordinate with heparin-binding residues in the N-terminal domain The cluster of hydrophobic ... demonstrated in a molecular modeling experiment that the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional ... study concluded that HSPG binding at the cell surface is required for maintaining LPL stability LPL’s C-terminal domain also appears to be involved in the binding of the enzyme to receptors such...

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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

Ngày tải lên : 06/03/2014, 00:21

... monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction between monomers also involves portion of chains D and E of ... the ﬁrst and last turns of helices E and F, and the C-terminus helices I and J In addition, there is a lysine-rich N- and C-terminus, in accordance with the basic isoelectric point of CagS However, ... H pylori strains harbouring a functional cagPAI, is the activation of nuclear transcription factor-jB and the induction of pro -in ammatory production of cytokines, mainly interleukin (IL)-8 [12]...

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Báo cáo khoa học: Identiﬁcation, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

Ngày tải lên : 07/03/2014, 12:20

... substrate for fatty acylation and, thereby, be involved in membrane anchoring of the pilin Since PilA4 is similar to the meningococcal pilin and contains several central serine residues, it is tempting ... amounts in the inner and outer membranes The presence of PilQ in inner membranes is interesting, because secretin-like proteins of type IV pili and type II protein translocation machineries are ... for biogenesis of the DNA translocator and ⁄ or is involved in the stabilization of PilQ and PilA4 proteins in the outer membrane Moreover, the absence of any PilW-like proteins in the transformation...

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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Ngày tải lên : 07/03/2014, 15:20

... extracellular domain of corticotropin 2830 A Pisarchik and A Slominski (Eur J Biochem 271) 30 31 32 33 releasing factor-R1 contains major binding determinants for urocortin and astressin Endocrinology ... according to the isoform they contained For example, pCRFR1a corresponds to the plasmid containing CRFR1a isoform We also constructed an artificial CRFR1h2 isoform by introduction of two point mutations ... urocortin The artificial isoform (CRFR1h2) with the insertion of 37 amino acids between ligand binding domain and the first extracellular loop was able to produce 2828 A Pisarchik and A Slominski...

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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Ngày tải lên : 07/03/2014, 16:20

... in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP-binding sites were tested for interaction with the binding partner in ... biogenesis remains unknown In this study we mapped the mutual binding sites and assayed the effects of deletion and point mutations in Pex1p and Pex6p for the interaction of the proteins and for their ... the inverse importance of D1 and D2 in Pex1p and Pex6p for the interaction of these two proteins While a fragment of Pex6p consisting of N and D1 alone exhibits signiﬁcant binding properties for...

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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx

Ngày tải lên : 07/03/2014, 16:20

... also contains a GAL4 gene under the control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the ... odorants in two strains expressing either I7 OR and Golf, or I7 OR and Ga15 Strains were grown in minimal media with 2% glucose (uninduced) or subsequently in minimal media with 2% galactose (induced) ... expression is obtained in induced strains at 15 °C while the levels of Golf and Ga15 not change signiﬁcantly In addition, the protein levels of I7 OR, Golf and Ga15 in strains from Fig 4A were...

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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Ngày tải lên : 07/03/2014, 21:20

... protein used in this assay indicating that an equal amount of protein was used in all our samples and that the lack of interaction between VBARP and Nef is not due to the lack of input proteins ... variant A Fig Interaction of Vpr and VBARP proteins in vitro and in vivo (A) In vitro translated 35S-labeled VBARP-His isoforms were incubated with Vpr or Nef and immunoprecipitated using a-His (lanes ... Protein Analysis System (expasy; http://lsexpasy.org/tools) proteomics server of the Swiss Institute of Bioinformatics were used for in sillico characterization of VBARP, including prediction of...

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Báo cáo khoa học: Sulfation of hydroxychlorobiphenyls Molecular cloning, expression, and functional characterization of zebraﬁsh SULT1 sulfotransferases docx

Ngày tải lên : 08/03/2014, 02:20

... protein was detected, indicating Fig (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs ... Sepharose, and the bound GST fusion protein was treated with mL of a thrombin digestion buffer (50 mM Tris/HCl pH 8.0, 150 mM NaCl, 2.5 mM CaCl2) containing UÆmL)1 bovine thrombin Following a 30-min incubation ... tyrosineO-sulphate-binding protein and tyrosine-sulphated proteins in bovine liver membrane lysates Biochem J 275, 259–262 16 Sanger, F., Nicklen, S & Coulson, A.R (1977) DNA sequencing with chain-terminating...

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