biochemical regulation of lysine and threonine biosynthesis
Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc
Ngày tải lên :
20/02/2014, 02:21
... between the methionine and threonine biosynthesis pathways occurs at the level of homoserine and involves dierent enzymes and dierent allosteric patterns [8] of methionine (Fig 1) and can be considered ... input variables and to explain the behaviour of the branch-point in terms of the kinetic properties of CGS and TS Sensitivity analysis In a rst analysis, uxes of cystathionine and threonine (Fig ... independent of what happens on the cystathionine side The Phser branch-point combines the divergence of two uxes (uxes of cystathionine and threonine) with the convergence of two uxes (uxes of Phser and...
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Regulation of navigation and vessel-source pollution in the Northern Sea Route - Article 234 and state practice
Ngày tải lên :
01/11/2013, 09:20
... 15 of the Economic Zone Edict; Arts and 15 of the Environmental Edict; and Art 11 of the Decree of the Council of Ministers of the USSR, June 1990, ‘On Measures of Securing the Implementation of ... owner of a vessel registered with a state party and carrying over 2,000 tons 87 88 89 90 91 92 94 95 See Arts 1(4), and 7(4) of the NSR Regulations; Art 14 of the Economic Zone Edict; and Art of ... shall establish periods and areas closed for navigation, and also other measures ensuring the safety of shipping and the prevention, reduction, and control of pollution of the marine environment...
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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc
Ngày tải lên :
16/02/2014, 09:20
... tumorigenesis and genetic diseases [47] Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene ... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... A.-K Wenke and A.-K Bosserhoff stage of development They found that AP-2a, AP-2b, AP-2c and AP-2e are differentially expressed during the preimplantation period, and, with the exception of AP-2a,...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... polymerases and components of the transcription machinery in the nucleus The role of actin in transcription and its regulation Actin is both a major cytoskeletal component of all eukaryotic cells and ... component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA-binding proteins [4,22]; and (h) actin is a component of PICs and depletion of actin prevents ... actin monomers and prevents Rho-dependent nuclear accumulation of MRTF-A and SRF Actin and ABPs in transcription regulation activation [46], blocks the nuclear accumulation of MRTF-A and -B in the...
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Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx
Ngày tải lên :
07/03/2014, 21:20
... Kroger ¨ the 5¢-UTR and 3¢-UTR of CfNR allow control of both the timing and level of expression of introduced genes in transgenic C fusiformis Results Increasing the efficiency of C fusiformis transformation ... 3C) However, at molar ratios of nitrate to ammonium of 10 (Q10) and 25 (Q25), the levels of GFP fluorescence were 44% and 83%, respectively, of the fluorescence levels of nitrate-grown cells Thus, ... mixtures of ammonium and nitrate At relative molar concentrations of nitrate vs ammonium of 50 (Q50) and 100 (Q100), GFP fluorescence levels were virtually indistinguishable from fluorescence levels of...
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Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
Ngày tải lên :
23/03/2014, 07:20
... nucleus Regulation of gene expression by HIPPI Caspase Nuclear pore complex HIP1 Fig Possible mechanisms of regulation of transcription and apoptosis by HIPPI and HIP-1 in HD Interaction of HIP-1 ... transcription caspase-6, and caspase-3 Cleavage of Bid and release of cytochrome c and apoptosis-inducing factors from the mitochondria are also observed Coexpression of wild-type htt exon and Hippi decreases ... models, brains of transgenic animals, and post-mortem brains of HD patients [2] Aggregate formation is enhanced with the increase in the number of glutamines in vitro and in vivo, and is believed...
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Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
Ngày tải lên :
31/03/2014, 08:20
... (Fig 10, lane and 2) and wild-type (Fig 10, lane and 4) strains bearing multiple copies of MRG19 and grown in the presence of glucose (Fig 10, lane and 3) and glycerol (Fig 10, lane and 4) It is ... Expression of Mrg19p as a function of cell density Wild-type cells transformed with multiple copies of MRG19 (lane and 3) and wild-type cells (lanes and 5) were allowed to grow to an OD600 of 0.5 and ... we monitored the rate of oxygen uptake in log and stationary phase cultures of wild-type and MRG19 disruptant cells The rate of oxygen uptake was increased in wild-type and MRG19 disruptant cells...
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Báo cáo y học: "α Impact of VIP and cAMP on the regulation of TNF-α and IL-10 production: implications for rheumatoid arthritis" pps
Ngày tải lên :
09/08/2014, 01:23
... presence of VIP (lane 2), and that the combination of VIP and rolipram activates both CREB and ATF-1 upon LPS stimulation (lane 3) α VIP fails to modulate T-cell production of IL-10, TNF-α, γ and ... mouse, the augmentation of IL-10 and downregulation of TNF-α and IFN-γ results in a Th2 cytokine profile that is dependent on cell types present, differentiation status, and the activation stimulus ... cells, and synovial cells — all of which play an important role in the pathology of RA — and compare the findings with murine VIP data already published Materials and methods Reagents Capture and...
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Báo cáo y học: "T-cell contact-dependent regulation of CC and CXC chemokine production in monocytes through differential involvement of NFκB: implications for rheumatoid arthritis" docx
Ngày tải lên :
09/08/2014, 08:23
... concentrations of the following: 25 ng/ml TNFα (gift from Dr W Stec, Centre of Macromolecular Studies, Lodz, Poland), 100 ng/ml IL-6 (gift from Dr P Ramage, Sandoz, Pharma Ltd., Basel, Switzerland) and ... production of high levels of chemokines in M-CSF-differentiated human monocytes (macrophages) Levels of both CC chemokines (MCP-1, MIP-1α, MIP-1β and RANTES) and CXC chemokines (IL-8, GROα and IP-10) ... RANTES and MCP-1 was unaffected (Figure 2c) This inverted pattern of utilization of NFκB for the T-cell contact-dependent induction of CC and CXC chemokines in monocytes is surprising and indicates...
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Báo cáo y học: "Composition and regulation of maternal and zygotic transcriptomes reflects species-specific reproductive mode" doc
Ngày tải lên :
09/08/2014, 20:22
... ranking of IGR length between chicken and human, and another for its fold change between mouse and human Comparison of fold changes of all-maternal one-to-one orthologs versus the set of all one-to-one ... Materials and methods) To compare the relative contribution of post-transcriptional regulation among different classes of maternal transcripts, we used the length of the 3' UTR as an estimate of the ... strict-zygotic, P < 10-4 and P < 10-3; the genome-wide set of all core and metazoan genes, P < 10-21 and P < 10-72) Similarity in regulatory architecture of maternal and zygotic genes across...
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Ceftriaxone-induced up-regulation of cortical and striatal GLT1 in the R6/2 model of Huntington’s disease pps
Ngày tải lên :
10/08/2014, 05:21
... to food and water ad libitum Both the housing and experimental use of animals followed the National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use ... WTs and R6/2s indicate saline treatment, and WTc and R6/2c indicate ceftriaxone treatment N refers to number of animals per group Genotype and CAG repeat length We used PCR for genotyping and ... analyses and genotyping, and helped with data interpretation BRM participated in study design, and helped with data collection and analysis GVR conceptualized and designed the study, and revised...
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Báo cáo y học: "Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate" pps
Ngày tải lên :
11/08/2014, 03:20
... on apoptosis and priming of oxidative burst activity We report differential effects of DFP on neutrophil apoptosis and priming of oxidative burst activity, reflected in suppression of constitutive ... adding 70 μL of 2.8 mM TMB in PBS and 60 μL of mM hydrogen peroxide After minute of incubation at room temperature, the reaction was blocked with 50 μL of stop solution (500 μL of 10 mM sodium ... μg/mL) and analyzed using a BD FACS CANTO Flow Cytometer Data were analyzed using BD FACS DIVA software A minimum of 10 000 events were collected and analyzed at excitation wavelength of 488 nm and...
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Báo cáo y học: "Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate" pptx
Ngày tải lên :
11/08/2014, 06:22
... on apoptosis and priming of oxidative burst activity We report differential effects of DFP on neutrophil apoptosis and priming of oxidative burst activity, reflected in suppression of constitutive ... adding 70 μL of 2.8 mM TMB in PBS and 60 μL of mM hydrogen peroxide After minute of incubation at room temperature, the reaction was blocked with 50 μL of stop solution (500 μL of 10 mM sodium ... μg/mL) and analyzed using a BD FACS CANTO Flow Cytometer Data were analyzed using BD FACS DIVA software A minimum of 10 000 events were collected and analyzed at excitation wavelength of 488 nm and...
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Báo cáo y học: "Advances in understanding the regulation of apoptosis and mitosis by peroxisome-proliferator activated receptors in pre-clinical models: relevance for human health and disease" potx
Ngày tải lên :
13/08/2014, 13:20
... PPAR ligands seen in pre-clinical models of efficacy and safety in the context of human health and disease Peroxisome proliferator-activated receptors: structure, ligands, expression and target ... PPARα ligands such as Wy-14,643, ciprofibrate and clofibrate are known to produce peroxisome proliferation and liver tumors in rats and mice [75,76] However, since PP belong to the class of carcinogens ... Pennie W, Hedley K, Soames A, Kimber I and Roberts RA Downregulation of Lactoferrin by PPARalpha Ligands: Role in Perturbation of Hepatocyte Proliferation and Apoptosis Toxicol Sci 2002, 68:304-313...
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pelaez - regulation of banks and finance; theory and policy after the credit crisis (2009)
Ngày tải lên :
03/11/2014, 19:19
... a model of linear demand and supply and a 10.1057/9780230251250 - Regulation of Banks and Finance, Carlos M Peláez and Carlos A Peláez The Theory of Regulation and Finance 31 rectangle of gain ... and services by a system of simultaneous equations of demand functions by consumers, supply functions by producers, and identities of demand and supply The arguments of the demand functions of ... 10.1057/9780230251250 - Regulation of Banks and Finance, Carlos M Peláez and Carlos A Peláez The Theory of Regulation and Finance The sum of the inequalities results in the left-hand side of prices multiplying...
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Epigenetic regulation of normal and malignant hematopoiesis
Ngày tải lên :
05/09/2015, 11:25
... normal hematopoiesis and leukemia The methylation of histones on lysine and arginine residues by HMTs represents another level of gene regulation, and is probably the most complex of the epigenetic ... activities of HATs, which catalyse the transfer of acetyl groups from acetyl-CoA to lysine residues of target proteins, and HDACs, which catalyse the removal of acetyl groups The ability of histone ... mSin3A and HDAC1 in MEL and human T-ALL cells was linked to transcriptional repression and inhibition of erythroid differentiation (Huang and Brandt, 2000) The association between TAL1/SCL and mSin3A/...
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Manipulation of cyanogenesis and starch biosynthesis in cassava
Ngày tải lên :
16/09/2015, 17:11
... parts of many crops and an understanding of regulation of its biosynthesis will aid to make directed changes in composition/quality Second, increase in human population and shrinking land and water ... various stages of growth Effects of concentration of urea on gelatinization of starch granules from the cassava plants Effect of M urea on the swelling of starch granules of wild-type and mutant ... MALDI-TOF-MS spectrum of starch samples from 5-month-old PRC 60a and mutant S9 plants MALDI-TOF-MS spectrum of starch samples from 7-month-old PRC 60a and mutant S9 plants MALDI-TOF-MS spectrum of...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
Ngày tải lên :
19/02/2014, 13:20
... acetate, and 10.8 mM IPP and 10.8 mM DMAPP, respectively; *, internal standard (acetate) FMN, we observed the appearance of the signals of both methyl groups and of the methine group of the enzymatically ... Salmonella and Shigella sp.) of the proteobacteria group 10) are devoid of genes for the biosynthesis of IPP and DMAPP The same is true for the genome of the archaeon N equitans No putative orthologs of ... A vinelandii and P luminescens and to Idi-1 protein of the Bacteroid C hutchinsonii (expect values 3e-22, 5e-19 and 8e-20, respectively) Phylogenetic pattern of Idi proteins Paralogs of Idi-2...
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Báo cáo Y học: Agmatine oxidation by copper amine oxidase Biosynthesis and biochemical characterization of N-amidino-2-hydroxypyrrolidine pdf
Ngày tải lên :
17/03/2014, 23:20
... stoichiometric analysis of the enzymatic oxidation of agmatine yields a molar ratio of substrate (i.e agmatine) to O2 and H2O2 of : : Figure shows the 1H-NMR spectra of agmatine before (Fig 2A) and after (Fig ... time (% 15 s) Values of the first-order rate-limiting catalytic constant (kcat) and of the Michaelis constant, as determined in the absence and presence of the inhibitor (K and K app , m m respectively), ... mixing time (% 15 s) Values of the first-order rate-limiting catalytic constant (kcat) and of the Michaelis constant determined in the absence and presence of the inhibitor (K and K app , m m respectively)...
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Báo cáo khoa học: Regulation of maize lysine metabolism and endosperm protein synthesis by opaque and floury mutations potx
Ngày tải lên :
23/03/2014, 15:21
... protein)1) and SDH specific activity (nmol NAD + reducedÆmin)1Æmg protein)1) were determined in extracts of 20 DAP maize endosperms and following the addition of lysine (L) and/ or threonine (T) Standard ... endosperm and the protein was shown to activate the transcription of the 22 kDa a-zein [22] and 14 kDa b-zein genes [23], together with the b-32 [24] and cyPPDK1 (one of two cytosolic isoforms of pyruvate ... between that of Oh43fl1, similar to that of the wild type, and that of Oh43o2 From these results it was possible to assess the importance of lysine- rich nonzeins with accuracy, because of the quantitation...
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