binding of effector molecules
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
Ngày tải lên :
18/02/2014, 13:20
... active
pumping of the agents out of the cells and their pas-
sive uptake [10,31] and (b) competition between the
active pumping of the agents out of the cells and their
intracellular binding to receptors ... (2001) Characterization of binding of
leukotriene C4 by human multidrug resistance protein
1. Evidence of differential interactions with NH2-and
COOH-proximal halves of the protein. J Biol Chem
276, ... Subsequently, all of the upper phase, including
part of the oil cushion, was removed, leaving a fraction of
the oil above the cell pellets. The cell pellets were dissolved
by addition of 0.1 mL of guanidine...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
Ngày tải lên :
18/02/2014, 16:20
... amount of precipitation in
the presence of high concentrations of Arg-HCl
in the absence of the chaperone precluded analysis of
the effect of this concentration on the protective
ability of aB-crystallin.
Reduction-induced ... most likely
employs various methods of binding (or binding
modes) in order to prevent the aggregation of stressed
proteins. Some of these binding modes (or binding
sites) are favoured by phosphorylation ... the amount of precipita-
tion of catalase by 190 ± 5% (Fig. 3A). The presence
of aB-crystallin at a 1.0 : 0.5 w ⁄ w ratio of cata-
lase : aB-crystallin inhibited the precipitation of cata-
lase...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
Ngày tải lên :
18/02/2014, 17:20
... Discussion
Action of reversible effectors on AAA activity of
BuChE
The investigation of the effects of the ligands
(tyramine, serotonin and benzalkonium) on the AAA
and esterase activities of BuChE was ... wild-type enzyme
reflects the binding of benzalkonium to PAS.
Effects of benzalkonium on ASCh hydrolysis
Under our experimental conditions, a study of the
inhibition of ASCh hydrolysis was not possible
because ... higher
conformational plasticity of the active site gorge of
D70G compared with that of the wild-type enzyme for
acylation with neutral ester.
Effects of benzalkonium on ATMA hydrolysis
Hydrolysis of ATMA by wild-type...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
Ngày tải lên :
19/02/2014, 02:20
... indicated.
(B) The glycoforms of the protein bands were analyzed by calcula-
tion of the percentages of the di- (d), mono- (j) and nonglycosy-
lated (m) isoform as arithmetic means of separate gel runs. ... (B) Signal intensities of the di- (d), mono- (j)
and nonglycosylated isoform (m) of PrP
C
were quantified and calcu-
lated as percentages of the total signal. The glycoforms of the pro-
tein bands ... the
di-glycosylated isoform of PrP
C
than did antibodies to
the structured core region. However, the glycoform
patterns of mouse PrP
C
always showed the highest sig-
nal intensity of the di-glycosylated isoform,...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
Ngày tải lên :
19/02/2014, 05:20
... targets).
Effects of DNA cis-platination on sequence-
specific DNA binding of p73 proteins
We tested the influence of DNA modification with
cisplatin on the binding of two p73 isoforms, p73d and
p73b, ... inhibits
p53 binding to a synthetic p53 DNA -binding site. Here we demonstrate
that the effects of global DNA modification with cisplatin on binding of
the p53 or p73 proteins to various p53 DNA -binding ... probe
(Fig. 6A). Addition of either of the unmodified frag-
ments (70 ng per sample) resulted in a partial decrease
(by 35–45%) of the R
53
band intensities due to binding
of a portion of the p53 molecule...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
Ngày tải lên :
19/02/2014, 05:20
... phospholipid and membrane binding of the
other members of the PEBP family. Consequently, the
binding characteristics of PEBP proteins, including
those of the binding of I
C
to phospholipid membranes,
are ... PtdIns(3,4,5)P
3
.
Involvement of the CPY -binding sites of I
C
in its
membrane binding
As I
C
–CPY has no affinity for phospholipid membranes,
to obtain additional information on the involvement of
the CPY -binding sites of ... membranes.
Results
Membrane -binding properties of I
C
In an attempt to detect and characterize the membrane
binding of I
C
, a member of the PEBP family, we first
performed a liposome -binding assay of this inhibitor
for...
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Tài liệu Báo cáo khoa học: Inorganic phosphate regulates the binding of cofilin to actin filaments pdf
Ngày tải lên :
19/02/2014, 07:20
... regulation
of actin dynamics in the cell.
Results
Fluorescence measurements of the effect of Pi
on the rate of cofilin binding to TRC–F-actin
A convenient way to study the binding of cofilin to
F-actin ... fluorescence intensity of TRC–F-actin is
decreased > 70% upon binding of cofilin, while the
fluorescence intensity of G-actin changes little upon
addition of cofilin [21]. The binding of 5 lm cofilin ... our observations, the rate
of cofilin binding to F-actin in the presence of
2–30 mm Pi is the same at pH 6.5 and 8.0. The influ-
ence of Pi on the relative rates of cofilin binding at
these two pH...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
Ngày tải lên :
19/02/2014, 13:20
... complex [20]. Hence, the
mechanism of binding of fosfomycin by reinforcement of an
initial binding site (mainly K22 and R397) through recruit-
ment of secondary binding partners located in a flexible
loop ... involved in fosfomycin
binding, and in view of the multitude of interactions, it was
assumed that deletion of a single interaction would not lead
to a complete loss of fosfomycin binding. Therefore ... evidence from studies of small angle
X-ray scattering [14] and the protective effect of UDPNAG
on proteolysis of MurA [15]. Here we report the thermo-
dynamic profile of UDPNAG binding to two MurA...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... retarda-
tion. Iron loading of HepG2 cells resulted in increased expression of
Nor3.2-reactive CD1d molecules at the plasma membrane. Expression of
classical MHC class I and II molecules, ICAM-1 and ... appearance of
Nor3.2-reactive CD1d molecules at the cell surface
of iron-loaded HepG2 cells could be a consequence of
CD1d misfolding in intracellular compartments with a
subsequent release of the ... of CD1d molecules at the cell
surface of iron-loaded HepG2 cells in a non-native
form may have implications at two different levels; at
the level of the hepatic cell itself and at the level of...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
Ngày tải lên :
19/02/2014, 17:20
... in
favour of a specific binding of phosphate to cyto-
chrome c
3
[23] instead of a simple electrostatic effect
of increased ionic strength at least up to 0.2 m concen-
tration. The region of positively ... approximation of
the redox centres of the donor and acceptor, and that
the reduction potentials ensure a favourable driving
force, which is one of the main determinants of the
rate of electron ... Experimental measure-
ments of the reduction potentials of proteins involved
in complexes have been reported [5–7], but the effect
of partner binding on the microscopic properties of the
redox centres...
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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells pdf
Ngày tải lên :
19/02/2014, 18:20
... the
amplification of the 3¢ end of the b-gal gene. The presence
of similar amounts of genomic DNA in the reactions was
controlled by concomitant addition of primers that amplify
a 200 bp region of the RNA ... isolation by FACS of living cells expressing low
levels of antigen.
Discussion
The potential of scFvs for demonstrating the presence
of targeted protein antigens in the cytoplasm of mam-
malian ... intracellular
forms of antigen.
To analyze how these scFvs linked to EGFP or
d1-EGFP behave in case of low and constant expres-
sion of b-gal, we took advantage of the availability of
two cell lines,...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... using deletion mutants of
the 3¢ terminus of the minus-strand RNA but deletions
were made on the basis of the structure of the 5¢UTR of
the plus-RNA, the structure of the 3¢-end of the minus-
strand ... 3’
domains of plus or minus-strand HCV RNA.
(B) RNA products synthesized by HCV NS5B
in the presence of heparin with wild-type or
5’ deleted (–)IRES RNA.
Binding and replication of 3¢-end of HCV ... changing
the Not1-Age 1 fragment of the pCV-UTR4 with the
corresponding fragment deleted from all nucleotides of SLB1
T. Astier-Gin et al. Binding and replication of 3¢-end of HCV minus RNA
FEBS Journal...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... Therefore, HP1
may not participate in the binding of LBR to sperm
chromatin in eggs. In t he case of the binding of LBR to
Fig. 7 . Suppression of the b inding of LBR fragments to chromatin by
phosphorylation ... of the
arginine-serine repeat-region in the N-terminal portion of
LBR by an LBR kinase inhibits the binding of p34 protein
Correspondence to T. Horigome, Department of Biochemistry,
Faculty of ... of binding of LBR to
chromatin, beads bearing LBR fragments were preincu-
bated with a DNA solution and then the binding to
chromatin was examined (Fig. 3, h atched c olumn s). T he
binding of...
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Tài liệu Báo cáo Y học: Binding of gelsolin domain 2 to actin An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin pptx
Ngày tải lên :
22/02/2014, 07:20
... ?–
1–10 No binding ND No binding [51]
18–28 No binding ND 3 m
M
a
84–103 No binding No binding 1–2 mM
a
112–125 No binding 50 mM 4 mM [51]
119–132 2.9 m
M 2.0 mM 12 mM [51]
347–365 2 m
M Binding ... Weeds, A.G. (1994)
Characterization of the F-actin binding domains of villin:
classification of F-actin binding proteins into two groups according
to their binding sites on actin. FEBS Lett. 338, ... F-actin binding of
domain 2 with respect to the F-actin severing and capping
activity of the whole gelsolin molecule.
Keywords: actin; actin -binding proteins; cofilin; gelsolin.
The organization of...
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Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
Ngày tải lên :
22/02/2014, 07:20
... bound enzyme
forms. Our study offers the first EPR description of
important catalytic states of the dioxygenation process of
flavonols and shows that the anaerobic binding of flavonols
produces clear ... alter the EPR spectra of the enzyme
indicating the absence of binding to the copper centre. From
the chemical structures of the tested flavonoids we conclude
that the presence of a free 3-OH group ... specificity of 2,3QD for flavonol binding has been
tested by anaerobically incubating the enzyme with different
flavonoids. The addition of a flavone (apigenin), of a
flavanonol (taxifolin) and of a flavan-3-ol...
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