behavior of real gases at low temperature
Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc
... includingcell-fate specification, regulation of differentiation, andcontrol of cell proliferation and movement [45].Recently, we have shown that activation of the heat-shock response in the ciliate T. ... followed by 45cycles of denaturation at 95 °C for 30 s and annealing andextension at 60 °C for 15 s each. Following amplification,melting curve analysis of the DNA was performed at tem-peratures ... that facilitate template nucleation of microtu-bules by the c-tubulin ring complex at cold temperatures.AbbreviationsqPCR, quantitative PCR; RATE, rapid amplification of telomeric ends; TuRC, tubulin...
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Báo cáo khoa học: ATPase activity of RecD is essential for growth of the Antarctic Pseudomonas syringae Lz4W at low temperature potx
... rate of ATP hydrolysis) for the reactions werecalculated. Km(ATP)was determined at a saturating concen-tration (1 lm) of ssDNA, and Km(DNA)was calculated at asaturating concentration of ... significance of the RecD-associated ATPase activity required during the growth of P. syringae at low temperature (4 °C). The very low (but non-zero) ATPase activity of RecD (approxi-mately 8–10% of the ... (Graphpad Software, San Diego, CA,USA). In each case, Vmax(maximal rate of ATP hydroly-sis), Km(ATP)(ATP concentration at the half-maximal rate of reaction) and Km(DNA)(DNA concentration at the...
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Tài liệu Dramatic Attenuation in Fiber Access Terminals at Low Temperatures doc
... research also reveals that theissue becomes more severe for cables exposed to initially high temperatures (greater than 50˚C)prior to their exposure to low temperatures. Attenuation loss can be as ... governing this application, ADC has concluded that insome - but not all - cases, loose tube OSP cable, dielectric or armored, can experience excessiveattenuation loss at low temperatures (0˚C to ... information from a 1998 Bellcore white paper that outlined similar problems withcertain fibers in varying temperature environments. The paper, entitled "Time- and Temperature- Dependent Material...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... right panel). At low pH, bkcatis larger thankcat, but at high pH bkcatis smaller than kcat.Thatistosay,A328W goes from substrate activation, at low pH, tosubstrate inhibition, at high pH. ... between bkcatand kcat at high pH is large relative to the difference at low pH, andthe difference between bkcat at high pH and bkcat at low pHis relatively large (Fig. 5). In all of these ... titratable, with pKavalues of 6.0–6.6, suggestingthat the catalytic histidine is protonated at pH 5. Theexistence of activity when the catalytic histidine is protonatedindicates that the catalytic-triad...
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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... that is thought to realistically mimicthe interior of the protein matrix.Urea-mediated denaturationThe urea-mediated denaturation of Ngb and Mb was fol-lowed by monitoring the ellipticity at ... systematic analysis of the effect of acid on thedenaturation of 20 small monomeric proteins revealedthat the exposure of a protein to an acid solution caninduce a wide range of conformational ... desired denaturant concen-tration for 5 h at 25 °C in order to attain equilibrium.Denaturation of holoNgb or holoMb was performed in20 mm Tris ⁄ HCl and 0.15 m NaCl (pH 7.0), and that of apoNgb...
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Báo cáo Y học: Characterization of a partially folded intermediate of stem bromelain at low pH ppt
... lessconstant, indicative of the presence of a non-native stableintermediate at low pH.The emission spectrum of stem bromelain at pH 7.0(Fig. 2) shows a maximum at 347 nm that suggests thatsome of the ... han that of t he possiblemolten globule state at pH 0.8 but greater than that of thenative state. Acrylamide quenching data clearly show thatstem bromelain at p H 2.0 is in an u nfolded state ... conformational state that isdifferent from the native state at pH 7.0 as well ascompletely unfolded state in the presence of 6MGdnHCl.Figure 3 shows the near UV CD spectra of the nativestate of...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... undifferentiated state of hESC is affected by exposure to low temperature. The degree of spontaneous differentiation of low temperature- exposed hESC colonies was assessed by morphological observations ... results demonstrated that the majority of hESC survived exposure to low temperature for extended durations. Spontaneous differentiation of hESC after exposure to low temperature Following serial ... chromosomal aberrations. 3. Results Survival rate of hESC after exposure to low temperature As seen in Table 1, the MTT assay yielded lower raw absorbance values upon incubation at 25oC and...
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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc
... limitedcapacities to cope with low temperature, plants fromtemperate climates, such as Arabidopsis thaliana, grow at low temperature and can increase their freezing tol-erance when exposed to low but nonfreezing ... correlation between changes in theregulation of central carbohydrate metabolism andfreezing tolerance [4,8]. In Arabidopsis, the development of leaves at low temperature causes reprogramming of Keywordsacclimation ... the rate of assimilate export tosink organs. A comprehensive overview of interconversion rates is pre-sented, from which accession-specific regulation strategies during exposureto low temperature...
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Maximum reproductive rate of fish at low population sizes potx
... obser-vations are not included in this analysis. The SPRF=0wascalculated using estimates of natural mortality, weight at age, and maturity at age. Maturity and weight at age wereusually estimated ... that often show no strong reduction of recruitment at low spawner abundances over the range of the observations.This erroneous belief is caused by the lack of attention paidto the information ... fishery-independent esti-mates of abundance, do not have a large amount of agingdata, and often assume that the population is at the unfishedequilibrium at the beginning of the fishery. It is critical...
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low temperature hydrothermal synthesis of monodispersed flower - like titanate nanosheets
... investigate the effect of treatment temperature. Upon thermal treatment at 500 °C for 1 h, the titanate nano-sheets were converted into anatase TiO2(Fig. 1f), with slightlydeformation of their structures ... titanate showed high photocatalytic activity for H2evolution from water splitting reaction. Moreover, the sample heat treated at 500 °C exhibited higherphotocatalytic activity than that of commercial ... size at rather low temperature. In this work, we report a simple hydrothermal approach forpreparation of spherical titanate nanosheets by a novel 2-stepmethod combining the synthesis of spherical...
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low - level detection of ethanol and h2s with temperature - modulated wo3 nanoparticle gas sensors
... identification by modulating temperatures of SnO2-based thick film sensors, Sens. Actuators B 43 (1997) 45–51.[25] S. Nakata, T. Nakamura, K. Kato, K. Yoshikawa, Discrimination andquantification of ... temperature profile for each type of sensor. In this way, measuring the response of one sensor at n different temperatures is similar to having an array of nsensors working at fixed temperature. In ... usedto generate the desired concentrations of ethanol and H2Sin synthetic dry air (80% N2and 20% O2) at a constantflow rate of 1 l/min.The working temperature of the sensor was modulatedby...
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Báo cáo khoa học: Variants of b2-microglobulin cleaved at lysine-58 retain the main conformational features of the native protein but are more conformationally heterogeneous and unstable at physiological temperature potx
... exclusionchromatography of dK58-b2m incubated at 310 K [10].The NMR data clearly indicate that the overall fold-ing pattern of the cleaved b2m variants is very similarto that of the wt protein. ... closesimilarity of the patterns is readily appreciated. Theexcellent resolution of the resonances in Fig. 10 couldnot be exploited for quantitation of the relative con-centrations of the two forms ... the expected pattern of the I2interme-diate that was unambiguously recognized in previousstudies of other b2m variants [21,22]. The best estimate of the equilibrium populations of the fully...
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Báo cáo khoa học: Structural adaptation to low temperatures ) analysis of the subunit interface of oligomeric psychrophilic enzymes pdf
... surfaces,rather than with water, decreases at low temperatures,because solvation of nonpolar surface is thermodynam-ically favored at low temperatures. This effect can leadto cold-induced denaturation, ... interactions isdiminished at low temperature. It should be consideredthat, at moderate temperatures, hydrophobic interac-tions are the most relevant forces for the preservation of enzyme structure. ... thatthe loss of activity at moderate temperatures might notbe always directly related to the loss of enzyme struc-ture). It is generally believed that cold adaptationresults from a combination...
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