... some sera to a 9- and a 15-kDa band We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av and the 15-kDa band shows reactivity ... from tomato extract using FPLC Two different isoforms of Lyc e were cloned and expressed in E coli and purified by electroelution Sera from German and Spanish patients with adverse reactions to tomato ... N-glycans containing xylose and fucose residues in the IgEresponse of tomato- allergic patients Materials and methods Preparation of allergen extract Extracts from tomatoand low fat milk were prepared...
... (quenching and xanthophylls cycle) and in the formation of abscisic acid Moreover, many species use them to make coloured flowers and fruits to attract pollinators and seed dispersers In tomato, ... of Psy1 and Psy2 genes and the transcription patterns of both genes in different tomato tissues The role of carotenoids as secondary metabolites in the pigmentation of tomato flowers and fruits ... results 528 Results Isolation of tomato Psy1 and Psy2 genes Using the sequences M8474 and L23424 reported in the NCBI database corresponding, respectively, to tomato Psy1 and Psy2 mRNAs, an extended...
... against the peptides LSGNAVLGDIGVHFK and EIYFEPTK, and produced a PCR fragment of 750 bp that was cloned and sequenced The coding sequence was completed by 5¢- and 3¢-RACE, yielding SoPFK1, which ... feature of chloroplast ATP-PFK [27,28,32], and in contrast to the stimulation of the cytosolic isoenzyme observed in spinachand other plants [27] These Fig Spinach PFK activity assayed in the absence ... Gly105 and Lys124, in the Esc coli enzyme [45] are conserved in the atypical and previously uncharacterized ATP-dependent PFK sequences [3,4,6,39], and they are also conserved in SoPFK1 and SoPFK2...
... both JA and MeJA are published, the ratios depend on the plant species and the tissue analysed and vary from : (JA/MeJA) in Arabidopsis leaves [4] to about 10 : in tomato flowers [38] For tomato ... at 94 °C, at 40 °C and at 72 °C, followed by 30 cycles of 30 s at 94 °C, at 45 °C and at 72 °C Gel electrophoresis, DNA elution and modification was carried out according to standard protocols [24] ... 5¢- and 3¢-RACE, respectively Sequencing and annealing of the 5¢ and the 3¢ sequence revealed an ORF of 789 bp, encoding a 262 amino acid protein (Fig 1) All four peptides from the purified tomato...
... –Pi tomato suspension cells MATERIALS AND METHODS Chemicals and plant material – Fractogel EMD-SO3650 (S) cation exchange resin, and KCl were from BDH Chemicals A Superose 12 HR10/30 column and ... competitive Inhibition of SAP1 and SAP2 by molybdate and fluoride was competitive and mixed, respectively (Table 4) Moreover, I50 and Ki values of SAP2 for molybdate, fluoride, and Pi were generally lower ... inhibition pattern and enzyme-inhibitor dissociation constants for selected inhibitors of SAP1 and SAP2 I50 values, patterns of inhibition and Ki and Ki¢ values (representing competitive and uncompetitive...
... signals at 1721 and 1698 cm)1 and negative ones at 1690 and 1683 cm)1 (shoulder) In addition, there are reproducible positive bands at 1748, 1733 and 1710 cm)1 as well as negative bands at 1754/1755, ... cm)1 (negative) and 1719 cm)1 (positive) In addition, positive bands are observed at 1750–1753, 1736/ 1738, 1703–1706, and 1688 cm)1 and negative bands at 1743/1745, 1706–1710, and 1678–1681 cm)1, ... with model compounds and conclusions drawn from the bacterial reaction centre [12] Thus, the bands at 1644 and 1632 cm)1 have been tentatively assigned to QA vibrations and the band at 1478 cm)1...
... (m) The free energy and the standard volume change are 3.94 kcalÆmol)1 and )66.1 mLÆmol)1, in good agreement with that obtained from fluorescence experiments (3.9 kcalÆmol)1 and )72 mLÆmol)1) Fig ... protein as a model because of its very low DG of unfolding at pH 6.0 and 20 °C ()3.5 kcalÆmol)1) and because of its large standard DV ()120 mLÆmol)1) The response of the 33-kDa protein to pressure ... protein was isolated and purified from spinach chloroplast photosystem II as described in our previous report [11] The purified protein was dialysed against 10 mM NH4HCO3 and then lyophilized The...
... it and add the tomatoes; boil up once, and pour into well sterilized pint jars and seal NO 47 TOMATO MARMALADE Use: quarts of ripe tomatoes, peeled and sliced lemons, cut in halves lengthwise and ... TOMATOES ANDBACON Toast rounds of bread; sprinkle generously with grated cheese; put a slice of tomato on each round, and two slices of bacon on top of the tomato; bake in quick oven until bacon ... flour; strain the tomatoes, and rub the pulp through a sieve; add the water, pepper, sugar, and salt, and put over fire; rub the butter and flour to a smooth paste, and stir into the tomato stock...
... (bacterial wilt disease) and yield of tomato grafted on eggplant rootstocks and non-grafted tomato For the Red River Delta, apart from problems of soil-borne diseases, tomato land is usually flooded, ... Agriculture and forestry (HUAF)- Central Coast Dalat Potato, Vegetable and Fruit Center (PVFC) - Central Highland Materials included: 13 hybrid tomato lines from AVRDC - the World Vegetable Center and ... characteristics - Insect pests and diseases - Yield components and yield (tons/ha) - Morphological characteristics and fruit quality Workshop on GAP for tomato, cucumber seed, seedling and field production...
... hybrid combinations of semideterminate tomatoand 62 hybrid combination of determinate tomato mported from Thailand and India and popular varieties of check Grandeva, DV269, HT42, VL2004 10 potential ... of tomao; Status of tomato production in the world and in Vietnam; Status of research on tomato in the world and in Vietnam Origin of Tomato Solanum lycopersicum is from Andean region including ... gai), contributing to diversity of tomato varieties and improving crop productivity and quality of commercial hybrid tomatoand promote development scale of tomato production with higher economic...
... responsible for tomato texture, such as tomato juiciness, granulosity, hardness and skin resistance The attributes belonging to the taste group (sweetness, sourness, saltiness, tomato flavour and pleasantness) ... juiciness, granulosity and hardness, and by equatorial diameter The fourth factor accounts for a further 11% of the variability, and consists in the Asn, Ser, Glu and Thr amino acids, and in skin resistance ... is used for the tomato ecotypes grown in the Sarno field; green, the tomato ecotypes grown in Ercolano; blue, the tomato ecotypes grown in Sorrento ascorbic and fumaric acids, and two agronomic...
... fifth landmark and occurred between 10 and 12 dpa (Fig 7E and 7F) highlighting the beginning of cotyledon growth The 13–16 dpa embryo was torpedo shape, marking the sixth landmark (Fig 7G and 7H) ... analysis CR and TM conducted the floral landmark study NW and TM conducted the fruit landmark study EvdK supervised the project and conducted the pollination experiment HX, TM, NW and EvdK wrote ... landmarks Flower Development Landmarks; Buzgo et al (2004) Days after flower initiation in tomato Perianth organs Reproductive organs Ovary and ovule development (1) Inflorescence formation and...
... stages Discussion Transcriptomic analysis of tomato carpel development and fruit set To identify the key steps and processes in tomato carpel development and fruit set, we analyzed the carpel transcriptome ... are relevant and asses the importance of GAs for parthenocarpic development and a new role for ethylene in this process Results Transcriptomic analysis of tomato carpel development and fruit set ... involved in tomato carpel development, fruit set and to find differences between normal fruit set and parthenocarpy We analyzed the genes regulated during normal carpel development and fruit set...
... similarities and differences among cultivated and wild tomato species, we mined the EST and unigene tomato database from SGN in search for substitutions and insertions/deletions between and within ... unigenes and perform gene evolution analysis and tests for positive selection We were able to suggest candidate genes and gene families that may be related to domestication of the cultivated tomato and/ or ... intraspecific SNPs in S lycopersicum (L) ESTs and interspecific SNPs between S lycopersicum and S habrochaites (LxH) and between S lycopersicum and S pennellii Perl and R [40] scripts were developed to...
... PSTVd-infected tomato (Viroid-infected tomato, lane 2), non-transformed control (X, lane 3), transgenic plants XS (lane 4) and XAS (lane 5), and tomatoes infected with Potato virus X (PVX-infected tomato, ... stunted, possessed thick, dark leaves, and exhibited reduced flowering and root formation, and XAS plants were taller and had increased numbers of inflorescences and flowers (Figure 3C) Morphometric ... dwarfing and reduced root formation, similar to symptoms of PSTVd infection of tomato plants Results from hormone supplement and gene expression studies suggest that gibberellic acid biosynthetic and/ or...
... aborted during development, and one in which stamens and/ or carpels are never initiated [17] Golenberg and Freeman [18] argued that floral organ identity genes, particularly B and C class genes, will ... both males and female [27] Later in development, SpAG expression is sex-specific and becomes restricted to the microsporangial cells in males and the nucellus in females In contrast, the spinach ... analyzed the function of the spinach B (SpPI and SpAP3) and C (SpAG) class genes by suppressing individual gene expression during floral development We demonstrate that both B and C class genes retain...
... VA(AC4M) and VA(AC2MAC4M)] of VA/ToLCV constructs in tomato plants (A) Total DNA was isolated from the tomato leaves agroinfiltrated with VIGS, VA(AC2M), VA(AC4M) and VA(AC2MAC4M) separately and the ... siRNAs were indeed formed in tomato The band intensities were quantified and were normalized with respect to respective loading controls The normalized values of the band intensity, setting arbitrarily ... replicates in both dicot and monocot plants Figure The viral amplicon construct, VA/pCAMBIA1391Z, replicates in both dicot and monocot plants The plants (Tomato, Tobacco, Arabidopsis and Rice) were agro-infiltrated...
... three exist for tomato (HindIII [12], MboI, and EcoRI) and two exist for potato (HindIII and EcoRI) The tomato libraries are available through the SOL Genomics Network (SGN) [13] and the potato ... species tomatoand potato, and the model plant organisms A thaliana and P trichocarpa Using similar approaches, microsynteny has been observed between A thaliana and B rapa [16]; C papaya and P ... and M acuminata and O sativa [22] A higher number of tomatoand potato BACs display microsynteny to P trichocarpa, than to A thaliana The reduced level of microsynteny between tomato/ potato and...