... 20 21 22 23 24 25 26 27 28 29 Structureof B1 2- dependent glycerol dehydratase (Eur J Biochem 26 9) 4493 coenzyme B1 2: X-ray structureof diol dehydratase-adeninylpentylcobalamin complex Structure ... dehydrataseÆcobalamin Ó FEBS 20 02Structureof B1 2- dependent glycerol dehydratase (Eur J Biochem 26 9) 4491 Fig Structures of the active site and the cobalamin-binding site (A) Stereo drawing of the ... is covered by the corrin ring of cobalamin that is bound on the interface of the a and b subunits Two a subunits form dimer a2 to which two b and two c subunits are bound separately This structure...
... Yoshizawa et al Structureof Bg7S, a XEGIP-like protein of soybean Structured digital abstract l Bg7S binds to Bg7S by x-ray crystallography (View interaction) l Bg7S binds to Bg7S by cosedimentation ... Journal 27 8 (20 11) 1944–1954 ª 20 11 The Authors Journal compilation ª 20 11 FEBS 1945 Structureof Bg7S, a XEGIP-like protein of soybean T Yoshizawa et al Fig Structureof Bg7S from soybean (A) ... supposedly stabilize the three-dimen- Structureof Bg7S, a XEGIP-like protein of soybean sional structureof Bg7S The Cys209–Cys418 bond seems to be significant for stabilization in particular, because...
... quality of protein structures J Appl Crystallogr 26 , 28 3 29 1 51 Lambert C, Leonard N, De Bolle X & Depiereux E (20 02) ESyPred3D: prediction of protein 3D structures Bioinformatics 18, 125 0– 125 6 52 ... (1991) Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteins Antimicrob Agents Chemother 35, 22 94– 23 01 D’Amico S, Sohier ... A & Zavodszky P (20 01) A better enzyme to cope with cold Comparative flexibility studies on psychrotrophic, mesophilic, and thermophilic IPMDHs J Biol Chem 27 6, 28 121 – 28 125 20 Kim S-Y, Hwang...
... Table 2. 3 Table 2. 3 Coordinating atoms of calcium and distances Calcium No Interacting Atoms ASP 44 OD1 ASP 46 OD1 ASP 48 OD1 ILE 50 O ASP 52 OD2 WAT 27 Distance in Å 2. 47 2. 49 2. 50 2. 45 2. 48 2. 70 ... negatively charged residues Asp 128 , Asp 224 , Glu260, and Asp330 that are known to be involved in substrate binding and catalysis (Abad et al, 20 02) 53 Table 2. 4 Exposure of charged residues on various ... experiments 2. 3 RESULTS 2. 3.1 Overview of AmyA structure AmyA consists of a single polypeptide chain of 488 residues and comprises a total of 18 α-helices and 20 β-strands The overall fold of AmyA consists...
... Waals’ contacts between the Tyr205 side chain and importin-a (Table 2) The numbers of contacts are comparable with those of the basic residues at P2 (Lys203: 31 contacts), P3 (Lys204: 20 contacts) ... NLS binding J Biol Chem 27 5, 21 218 21 223 Kobe B (1999) Autoinhibition by an internal nuclear localization signal revealed by the crystalstructureof mammalian importin alpha Nat Struct Biol ... MR, Corbett AH & Hodel AE (20 01) Dissection of a nuclear localization signal J Biol Chem 27 6, 1317– 1 325 Fontes MR, Teh T & Kobe B (20 00) Structural basis of recognition of monopartite and bipartite...
... FEBS Journal 27 4 (20 07) 3044–3054 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 3045 Crystalstructureof BcZBP from B cereus V E Fadouloglou et al A B Fig Structureof the BcZBP dimer (A) ... compilation ª 20 07 FEBS V E Fadouloglou et al Crystalstructureof BcZBP from B cereus Fig Model of the BcZBP–GlcNAc complex Stereoview of the energy minimized putative BcZBP–GlcNAc complex A slice ... Journal 27 4 (20 07) 3044–3054 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 3047 Crystalstructureof BcZBP from B cereus V E Fadouloglou et al Table Deacetylase activity of the BcZBP protein...
... [19 ,24 ,25 ]) SPRK from the psychrotrophic Serratia sp is not found to be considerable less stable than PRK [18], but VPRK is [26 ,27 ] This could possibly in part be related to the number of salt bridges ... (CI2; PDB 1lw6, 1tm1 and 2sni [29 –31]) or eglinC (PDB 1sbn [ 32] ) and subtilisin Carlsberg in complex with eglinC (PDB 1cse [33]), ovomucoid turkey inhibitor (OMTKY3; PDB 1r0r [34]) or the double-headed ... loop of chymotrypsin inhibitor II (CI2) in complex with subtilisin BPN (cyan, PDB 2sni), the inhibitor fragment of lactoferrin in complex with PRK (green, PDB 1bjr), a hepta-peptide in complex...
... Number of unique reflections Resolution range Overall completeness Overall Rmerge I/rI Redundancy 88 606 33 568 ˚ 8.0 2. 75 Aa 92. 4% ( 92. 8% )b 9 .2% (30.4% )b 11.8 (3.9 )b 2. 6 (2. 3 )b ˚ Dataset was collected ... 350 800 65 20 00 150 900 20 0 700 HOP Inhibition and site-directed mutagenesis of T brucei gGAPDH In the absence of a 3D structureof a complex of T brucei gGAPDH with an analogue of 1,3-BPGA, we ... differences may be responsible for the extended/bent conformation of inhibitors inside the active site: (a) substitution of Ser247 in T cruzi gGAPDH by Ala246 in T brucei gGAPDH; (b) different conformations...