0

b and aberrant immune responses

Control of Innate and Adaptive Immune Responses during Infectious Diseases ppt

Control of Innate and Adaptive Immune Responses during Infectious Diseases ppt

Sức khỏe giới tính

... Protection and Pathogen Evasion of Immune Response During Tuberculosis Andre Bafica and Julio Aliberti Abstract An integrated response of the host is essential in health and disease Upon microbial exposure, ... Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianopolis, SC, Brazil e-mail: andre.bafica@ufsc.br J Aliberti (ed.), Control of Innate and Adaptive Immune Responses ... cell responses await to be answered And, in addition, the use of the lipoxygenase system and its mediators by pathogenic microbes as a general mechanism for evasion and manipulation of immune responses...
  • 189
  • 1,399
  • 1
Báo cáo y học:

Báo cáo y học: "The role of leptin in innate and adaptive immune responses" doc

Báo cáo khoa học

... proliferation and IFN-γ production following the stimulation of lymph node cells by mBSA were significantly reduced in ob/ob and db/db mice In contrast, IL-10 production by lymph node cells from ob/ob and ... which is based on the induction of a local Arthus reaction by intraarticular injection of methylated bovine serum albumin (mBSA) into the knee joint of mBSA-immunized mice Ob/ob and db/db mice ... deficiency and leptin administration in disease models mediated by adaptive immune responses in mice Models WT mice Non-obese diabetic mice ob/ob mice ↑ Serum leptin before onset of diabetes ↓ Arthritis...
  • 10
  • 469
  • 0
Báo cáo y học:

Báo cáo y học: "Toll-like receptors and innate immune responses in systemic lupus" docx

Báo cáo khoa học

... pivotal roles in SLE TLR7 and TLR8 bind to single-stranded RNA, TLR3 to double-stranded RNA, and TLR to DNA These receptors are intracellular and are engaged by their ligands in a cytoplasmic compartment ... These and other observations discussed below indicate that innate immune recognition plays a key role in the initial development and amplification of the immune response to both nucleic acid and ... is activated by single-stranded RNA containing U and G rich motifs [17,18] Thus, highly U/G-rich U and Y RNAs bound respectively to snRNP and Ro/La autoantigens both stimulate TLR7 and/ or TLR8...
  • 7
  • 389
  • 0
Báo cáo sinh học:

Báo cáo sinh học: " DNA-vaccination via tattooing induces stronger humoral and cellular immune responses than intramuscular delivery supported by molecular adjuvants" ppt

Báo cáo khoa học

... detection antibody (BD Pharmingen) in 100 μl of PBS were added per well, and the plates were kept at 4°C overnight The plates were washed six times with PBS-0.01% Tween 20 and once with PBS, and this ... research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours ... tattooing has been shown to be higher than that after intradermal injection [7,8] and gene gun delivery [8] DNA vaccines delivered by tattoo were able to induce both cellular [6,7] and humoral...
  • 8
  • 206
  • 0
Báo cáo hóa học:

Báo cáo hóa học: " First-line chemoimmunotherapy in metastatic breast carcinoma: combination of paclitaxel and IMP321 (LAG-3Ig) enhances immune responses and antitumor activity" pptx

Hóa học - Dầu khí

... chemo-immunotherapy protocols To investigate the role of the immune response behind the above clinical results, we analyzed both the absolute numbers of PBMC subsets and any changes in the proportions of key constituents ... anti-LAG-3 mAb (1 7B4 ) diluted in neat human AB (Jacques Boy) was used as reference standard (see [13] for details) Pharmacodynamics Blood samples were collected pre-dosing at D1, D85 and D170 and directly ... (BD Trucount™ tubes, BD Biosciences) After lysis of red blood cells using the BD FACS lysing solution, cells were analyzed using a 6-color FACSCanto cytometer and the absolute number of cells per...
  • 11
  • 472
  • 0
báo cáo hóa học:

báo cáo hóa học: " Aspirin-triggered lipoxin A4 attenuates LPSinduced pro-inflammatory responses by inhibiting activation of NF-B and MAPKs in BV-2 microglial cells" pptx

Hóa học - Dầu khí

... were blocked with 3% BSA in PBS for h and incubated with rabbit anti-NF- B p65 antibodies Page of 12 (1:200, Santa Cruz Biotechnology, Santa Cruz) overnight at 4°C After washing, cells were incubated ... analyze NF- B (A) and AP-1 (B) DNA-binding activity by EMSA, as described in Methods Binding specificity was confirmed by unlabelled probe (100-fold in excess; lane 5) to compete with labelled oligonucleotide ... expression by ATL in LPS-stimulated BV-2 cells BV-2 cells were pretreated with ATL (1, 10 and 100 nM) for 30 in the absence or presence of 100 μM Boc-2 (30 before ATL treatment) followed by incubation...
  • 12
  • 414
  • 0
báo cáo hóa học:

báo cáo hóa học: " The impact of shift work induced chronic circadian disruption on IL-6 and TNF-a immune responses" pot

Hóa học - Dầu khí

... Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit ... completed by 225 shift workers and 137 daytime workers A medical examination was performed and biometrical data including age, gender, height, weight, waist and hip circumference and smoking habits ... sleep disturbances and chronic sleep debt [1-6] To investigate the long-term influence of chronic sleep debt and circadian disruption on the immune system we compared cytokine production and lymphocytes...
  • 5
  • 505
  • 0
Báo cáo khoa học:

Báo cáo khoa học: "Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex" docx

Báo cáo khoa học

... molecules, pp.47-70 In: Barta MHC, differentiation antigen, and cytokines in animal and birds Monographs in Animal Immunol., VA:BAR-1 AB Inc., Blacksburg, 1990 Fenner, F J., E P J Gibbs, F A Murphy, ... vaccine-induced neutralizing antibody titers in swine fever Vet Microbiol 1988, 16, 123-128 Timoney, J F., J H Gillespie, F W Scott, and J E Barlough Hagan and Brunners microbiology and infectious diseases ... domestically available, and boostered at weeks after the first vaccination Sera were collected at the same intervals from peripheral bloods and hog cholera virus-specific antibodies were detected by an indirect...
  • 4
  • 374
  • 0
Báo cáo y học:

Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps

Báo cáo khoa học

... (FACSCalibur, BD Biosciences) gated on CD11c+ and CD1 1b+ cells Analysis of the Dendritic Cell Population in Lymph Nodes BALB/c mice were treated as described above for BAL fluid isolation, and peribronchial ... described above, and the relative numbers of CD11c+ and CD1 1b+ cells were determined by flow cytometry CIN treatment decreased the number of CD11c +b+ cells and increased the number of CD11c +b2 cells ... Jackson Laboratory (Bar Harbor, ME) BALB/c mice have a predominantly Th2type response to allergens, and these were used for most of the experiments The transgenic C57BL/6TG(TcraTcrb)1100mjb/j mice...
  • 11
  • 486
  • 0
Báo cáo y học:

Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps

Báo cáo khoa học

... based on the murine IBD model described above and have, as with Tr1 cells, flagged the importance of IL-10 and TGF-β By blocking IL-10 signalling in vivo with monoclonal antibodies against the ... monoclonal-antibody-mediated blockade of TGF-β abrogates TR cell suppressive functions both in vivo and in vitro [52,53] Interestingly, TGF-β does not necessarily have to act as a soluble factor but can be ... state also seems to be closely related to TR cells’ suppressive ability because if it is broken there is a concomitant loss of regulatory activity both in vitro and in vivo [37] Table summarises what...
  • 7
  • 576
  • 0
Báo cáo y học:

Báo cáo y học: "Negative regulation of cytokine signaling and immune responses by SOCS proteins" potx

Báo cáo khoa học

... kinase/signal transduction and activators of transcription 1) pathway SOCS1 is probably induced by STAT1 and NF- B, and then suppresses both STAT1 and NF- B One possible mechanism of NF- B suppression is ... restored in T and B cells on a SOCS1–/– background (SOCS1–/– transgenic mice) In these mice, DCs were abnormally accumulated in the thymus and spleen and produced high levels of BAFF/BLyS and APRIL, ... system The SOCS box interacts with Elongins B and C, Cullin-5 or Cullin-2, Rbx-1 and E2 [24–26] Thus, CIS/SOCS family proteins, as well as other SOCS-box-containing molecules, probably function...
  • 11
  • 428
  • 0
Báo cáo y học:

Báo cáo y học: "Imbalanced effector and regulatory cytokine responses may underlie mycobacterial immune restoration disease" pot

Báo cáo khoa học

... infection by Mtb and non-tuberculous mycobacteria (NTM) is associated with increased cellular immune responses to PPD [1,2,5,7,17] Our controls were considered to be 'at risk' of developing IRD because ... in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page of (page number not ... of effector and regulatory cytokines, peripheral blood mononuclear cells (PBMC) from both IRD patients and from six non-IRD patients were cultured with antigens and a mitogen control and supernatants...
  • 5
  • 171
  • 0
Báo cáo y học:

Báo cáo y học: " Phytol-based novel adjuvants in vaccine formulation: 1. assessment of safety and efficacy during stimulation of humoral and cell-mediated immune responses" doc

Báo cáo khoa học

... cellmediated anti-tumor immunity, and autoimmune reactivity in BALB/c, C57Bl/6, and autoimmune-prone NZB mice Gender-matched, 8–12 weeks old BALB/c and C57Bl/6 mice were bred in the animal facility ... for by using Azostix (Bayer, Elkart, IN) and Multistix (Bayer, Elkart, IN) respectively As described pre- Page of 11 (page number not for citation purposes) Journal of Immune Based Therapies and ... vaccine adjuvants: present and future Vaccine 2002, 20(Suppl 3):S7-12 Gupta RK, Relyved ER, Lindblad EB, Bizzini B, Ben-Efraim S, Gupta CK: Adjuvant balance between toxicity and adjuvanticity Vaccine...
  • 11
  • 405
  • 0
Báo cáo khoa học:

Báo cáo khoa học: " Characterisation of immune responses and protective efficacy in mice after immunisation with Rift Valley Fever virus cDNA constructs" pot

Báo cáo khoa học

... bN bN1 bN2 bN3 bN1/2 bN2/3 a Eukaryotic expression expression b Prokaryotic subsequently used to coat the inner wall of Tefzel tubings according to the manufacturer's instructions (BioRad Laboratories, ... Protective subunit immunogens to RVFV from bacteria and recombinant vaccinia virus In The biology of negative stranded viruses Edited by: Mahy B, Kolakofsky D New York, NY: Elsevier; 1987:321-329 Besselaar ... as a vaccine Br J Exp Pathol 1949:1-16 Metwally S: Foreign animal diseases 7th edition Edited by: Brown C, Torres A Boca Raton, FL: Boca Publications Group, Inc; 2008:369-376 Botros B, Omar A,...
  • 10
  • 226
  • 0
Báo cáo y học:

Báo cáo y học: "Neonatal immune responses to TLR2 stimulation: Influence of maternal atopy on Foxp3 and IL-10 expression" pot

Báo cáo khoa học

... committee of the Brigham and Women's Hospital and Harvard Pilgrim Health Care, Boston, MA Isolation of CBMC and lymphocyte proliferation Cord blood samples were collected from the umbilical vein ... Rifas-Shiman SL, Lieberman ES, Kleinman KP, Lipshultz SE: Maternal age and other predictors of newborn blood pressure Journal of Pediatrics 2004, 144:240-5 Schaub B, Tantisira KG, Gibbons FK, He H, ... in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page of (page number not...
  • 9
  • 313
  • 0
Báo cáo y học:

Báo cáo y học: "trong mucosal immune responses in SIV infected macaques contribute to viral control and preserved CD4+ T-cell levels in blood and mucosal tissues" pps

Báo cáo khoa học

... (clone MAb11, BD Bioscience), and anti-IFN-g APC (clone B2 7, BD Bioscience) PBS-buffer containing 0.5% Saponin for 45 at 4°C After a final wash cells were resuspended in PBS and analyzed by flow ... stimulation in the CD4+ memory T-cell subset of PBMC (A) and BAL (B) as well as in the CD8+ memory T-cell subset of PBMC (C) and BAL (D) in controllers, progressors and uninfected animals Polyfunctional ... stimulation in the CD4+ memory T-cell subset of PBMC (A) and BAL (B) and in the CD8+ memory T-cell subset of PBMC (C) and BAL (D) in controllers, progressors and uninfected animals The right panels show...
  • 13
  • 253
  • 0
Understanding the adaptive immune responses against newly emerged viruses, SARS coronavirus and avian h5n1 influenza a virus 1

Understanding the adaptive immune responses against newly emerged viruses, SARS coronavirus and avian h5n1 influenza a virus 1

Thạc sĩ - Cao học

... Epstein-Barr virus transformed lymphoblastoid B cell lines and Dr Gijsbert Grotenbreg’s laboratory, NUS, Singapore for performing the HLA typing of the SARS individuals To all fellow laboratory members, ... Engineering and Biotechnology, India and Dr Q Duong Hong, Institute of Biotechnology, Hanoi, Vietnam, and Falko G Falkner, Baxter Bioscience, Austria for providing H5N1 virus-derived DNA constructs and ... of Microbiology, Tumor and Cell Biology, Karolinska Institute For the collaboration work on SARS memory T cell response, I would like to thank Prof Antonio Bertoletti and his lab members from...
  • 4
  • 172
  • 0
Understanding the adaptive immune responses against newly emerged viruses, SARS coronavirus and avian h5n1 influenza a virus 2

Understanding the adaptive immune responses against newly emerged viruses, SARS coronavirus and avian h5n1 influenza a virus 2

Thạc sĩ - Cao học

... against baculovirus-expressed recombinant HA in Balb/c mouse 5.3 102 107 Monoclonal antibody production against baculovirusexpressed recombinant HA 5.3.1 Characterization of monoclonal antibody mAb ... protein by mAb 9F4 111 IV 5.4 Neutralizing ability of mAb 9F4 5.4.1 Neutralizing ability against homologous strain 113 5.4.2 Cross neutralization ability against other H5N1 strains and other subtypes ... MONOCLONAL ANTIBODY, 9F4 6.1 Epitope mapping of 9F4 6.1.1 Binding to internal deletion and substitution mutants 6.2 120 Mechanism of inhibition 6.2.1 Virus binding and Hemagglutinin inhibition (HI)...
  • 13
  • 237
  • 0
Understanding the adaptive immune responses against newly emerged viruses, SARS coronavirus and avian h5n1 influenza a virus 3

Understanding the adaptive immune responses against newly emerged viruses, SARS coronavirus and avian h5n1 influenza a virus 3

Thạc sĩ - Cao học

... segments encode the PB1 (96 kDa), PB2 (87 kDa) and PA (85 kDa) proteins PB1 and PB2 are found to be basic and PA is acidic based on their behavior on isoelectric focusing gels (Horisberger, 1980) The ... added and incubated at 37 oC for h After incubation, plates were washed and incubated further with Φ-labelled goat anti-biotin antibodies (GABA) for h at 37 oC The plates were washed as before and ... contains the RNA-dependent RNA polymerase active site and interacts with PA and PB2 The PB2 subunit is responsible for cap binding and contains a bipartite nuclear localization signal at C terminus...
  • 179
  • 233
  • 0

Xem thêm