automated method for embryonic stem cell culture ý pdf

Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx

Ngày tải lên : 09/08/2014, 20:22

... percentage of euploid cells Embryonic stem cell differentiation The EB3 cells and the parental line E14 cells [43] were allowed to differentiate using the 'hanging drop' method [44,45] The differentiation ... repeats were performed for each biological replicate: this comprises gels for E6 and gels for E7 For each clone we ran three replicates for t0 (control) and three replicates for t48 hours Corresponding ... mES cells, this system allows the efficient and long-term overexpression of the transgene in a dose- and time-dependent manner It is therefore suitable for systematic expression of HSA21 cDNAs Cell...

18
460
0

Guidelines for Human Embryonic Stem Cell Research potx

Ngày tải lên : 06/03/2014, 15:20

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 22 Guidelines for Human Embryonic Stem Cell Research ern form—besides the application of the scientific method...

179
330
0

Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx

Ngày tải lên : 07/03/2014, 00:20

... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... in both wild-type cells and the two Ptb) ⁄ ) ES cell clones Scale bar: 100 lm (B) Cell proliferation assay Cells (5 · 104) were seeded (d0) and counted every day for days of culture The proliferation ... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral...

11
454
0

Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt

Ngày tải lên : 09/08/2014, 20:22

... source of cells to address this issue and elucidate the exact pathways and intermediates between the embryonic stem cell and the mature adipocyte In particular, mouse embryonic stem cells (mESCs) ... that the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose stem cell biology ... differentiation of embryonic stem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G: Differentiation of embryonic stem cells...

16
355
0

Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx

Ngày tải lên : 09/08/2014, 23:20

... pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics ... base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: whole ... Acknowledgements The authors thank Shawn Cokus and Weihong Yan for the data visualization on genome browser, Amander Clark for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli...

12
458
0

Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot

Ngày tải lên : 10/08/2014, 05:20

... human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells ... dendritic cells could be derived from hES-CD34+ cells Large numbers of these hES-DCs cells can now be cultured from a renewable source for use in cell and immune-based therapies Since these cells ... the primary target cells that need to be protected against HIV-1 infection Methods hES cell growth and propagation H1 human embryonic stem cell line (hES) was obtained from WiCell (Madison, Wisconsin)...

9
261
0

Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt

Ngày tải lên : 14/08/2014, 08:20

... crest cells and their lineages by BMP2 promoter-driven selection markers paves the way for ES cell- derived neural crest stem cell biology These cells are attractive candidates for future cell ... Analysis neural crest stem cell associated transcripts in BMP2+ cells Figure of Analysis of neural crest stem cell associated transcripts in BMP2+ cells (a) RT-PCR analysis for the representative ... differentiation of mouse embryonic stem cells into mesoderm and endoderm at the expense of neuroectoderm formation Stem Cells Dev 2005, 14:162-172 Ariizumi T, Asashima M: Control of the embryonic body...

28
288
0

Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx

Ngày tải lên : 14/08/2014, 08:20

... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... mouse embryonic stem cells Nat Genet 2006, 38:431-440 Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH: A protein interaction network for pluripotency of embryonic stem cells...

19
359
0

Báo cáo y học: "Genomic chart guiding embryonic stem cell cardiopoiesis" docx

Ngày tải lên : 14/08/2014, 08:20

... cardiopoietic transformation of embryonic stem cells Discussion Embryonic stem cells are developmentally malleable [32], giving rise to highly specialized and discrete phenotypes crucial to the formative ... control of a discrete biological process Materials and methods Stem cell culture and differentiation Murine CGR8 embryonic stem (ES) cells were cultured without a feeder layer in 7.5% fetal bovine ... From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research Nat Rev Genet 2006, 7:319-327 Srivastava D, Ivey KN: Potential of stemcell- based therapies Genome...

16
110
0

Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc

Ngày tải lên : 14/08/2014, 20:22

... J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic research ... categories adhesion, cell cycle, cell death, cell- cell signalling, cellular metabolism, development, stress response, signal transduction, transcription, and transport separately for upregulated and ... atlas for cardiomyocytes and will be useful for further studies to elucidate their role during developmental processes http://genomebiology.com/2007/8/4/R56 Materials and methods Embryonic stem cell...

19
321
0

Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx

Ngày tải lên : 14/08/2014, 20:22

... circuitry in human embryonic stem cells Cell 2005, 122:947-956 Kim J, Chu J, Shen X, Wang J, Orkin SH: An extended transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132:1049-1061 ... Sox2 via the Oct4/Sox2 complex in embryonic stem cells Mol Cell Biol 2005, 25:6031-6046 Danford T, Rolfe A, Gifford DK: GSE: a comprehensive database system for the representation, retrieval, ... which a stem cell maintains its identity, and how it can be directed to form different cell types Our work will allow for a more detailed examination of the components of this expanded stem cell...

14
205
0

Báo cáo y học: " Analysis of novel geometry-independent method for dialysis access pressure-flow monitoring" pdf

Ngày tải lên : 13/08/2014, 16:21

... the need for phase information (4) For a second expression, we have ΔP = C(QA)2, and we define Poff = C(QA)2 and Pon = C(QA - QB)2 as the ΔP for pump off and pump on, respectively Solving for the ... consistent for sensor needle distances 1, 2, and cm from the dialysis needle tips Results of real-time waveform information obtained during monitoring are shown in Figure The waveform information ... micro-electro-mechanical systems (MEMS) manufacturing method referred to as micro-electro-discharge machining (EDM) has been used for three-dimensional machining of cavities in needle shafts for MEMS sensor...

12
331
0

Dynamic mechanical stimulation for mesenchymal stem cell chondrogenesis in an elastomeric scaffold

Ngày tải lên : 09/09/2015, 08:12

... particular that of stem cells, and the biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage Tissue Engineering The aforementioned weaknesses ... encourage the investigation on stem cells with chondrogenic potential for articular cartilage repair Both pluripotent and multipotent stem cells have been reported for studying cartilage repair(Hwang ... outcome from BMSCs treatment Pluripotent cells utilized in cartilage tissue engineering include embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) (Medvedev et al., 2010,Oldershaw...

142
233
0

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1

Ngày tải lên : 09/09/2015, 17:54

... protein MEF Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... 1.6 A model for lincRNA integration into the molecular circuitry of embryonic stem cells 18 Figure 1.7 Paradigms for how lncRNAs function at the molecular level Figure 4.1 Stromal co -culture of ... (all from Gibco, Invitrogen) For consistency in culture conditions, cells were later switched to the chemically defined mTeSR medium (Stem Cell Technologies) The cells were maintained at 37 °C ...

83
381
0

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 2

Ngày tải lên : 09/09/2015, 17:54

1
214
0

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3

Ngày tải lên : 09/09/2015, 17:54

... indicating that lincRNAs maintain the pluripotent cell state The studies on lncRNAs and pluripotency were all performed in mouse embryonic stem cells (mESCs) Results from the mouse studies may ... differentially expressed lncRNAs LncRNAs that are important for maintenance of stem cell identity should be highly expressed in ES, while lncRNAs important for neuronal differentiation should be upregulated ... lncRNAs, which could possibly be important for pluripotency, neural stem cell identity, or neuronal differentiation RNA from the highly homogenous cell types derived from hESCs were hybridized...

55
327
0

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4

Ngày tải lên : 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation ... RMST knockdown in ReN-VM neural stem cells (Figures 7.4, 7.5 and 8.7) Knockdown of RMST appeared to shut down the neurogenic program of neural stem cells, with the cells adopting a glia lineage...

13
275
0

Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5

Ngày tải lên : 09/09/2015, 17:55

... The cell biology of neurogenesis Nat Rev Mol Cell Biol 6, 777-788 Gregg, C., and Weiss, S (2003) Generation of functional radial glial cells by embryonic and adult forebrain neural stem cells ... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... M.S., and Zeng, X (2010) Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K.,...

34
316
0

Human embryonic stem cell derivatives as cancer therapeutics

Ngày tải lên : 09/09/2015, 18:52

... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... dendritic cell Myeloid progenitor cells Mesenchymal stem cell Natural killer T cell Neural stem cell Poly(2-hydroxyethylmethacrylate) Protein phosphatase 1, regulatory subunit 12C Pluripotent stem cell ... observed 1.2 Adult stem cells and cancer therapy Stem cells, by definition, possess two main characteristics Stem cells are able to produce daughter cells that retain their stem cell potential (selfrenewal),...

166
294
0

Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation

Ngày tải lên : 10/09/2015, 09:08

... viable for studies aimed at deciphering the mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells ... 2: Materials and Methods 2.1 Cell culture U87, a human glioma cell line, and HeLa, a human cervical cancer cell line, were obtained from American Type Culture Collection and cultured in DMEM ... Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL Technologies...

146
511
0