at the crossroad of innate and adaptative immunity
Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... Disease Post-translation change Cataract Truncation Deamidation Oxidation Glycation Phosphorylation Racemization ⁄ isomerization Desmin-related myopathy Desmin-related cardiomyopathy Distal hereditary ... development of therapeutic applications such as the use of carnosine to disaggregate glycated a-crystallin [47] and employing agents that prevent post-translational changes [40] Cataract and a-crystallin ... and drugs The therapeutic application of sHSPs is further suggested by study of other molecular chaperones, with disruption of HSPs that protect deregulated intracellular signaling proteins and...
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Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human aB-crystallin docx
... the absence and presence of ATP at two different molar concentrations of MTB HSP 16.3 In the presence of ATP, the molecular chaperone effect of MTB HSP 16.3 was enhanced for aggregation of CS and ... observed the effects of different concentrations of MTB HSP 16.3 in the presence and absence of ATP on the aggregation of CS In the absence of added MTB HSP 16.3, aggregation of CS increased after ... in an ATP-dependent manner [24] and indicating the involvement of ATP in substrate release [25] In this study, the effect of ATP on recombinant MTB HSP 16.3 was compared with the effect of ATP...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... polymerization of intact actin and aggregation of heated actin Interaction of HSP25 with intact actin Fig Effect of heating on the kinetics of polymerization (A) and saltinduced increase of the light ... °C, and aggregation was initiated by the addition of KCl and MgCl2 up to 50 mM and mM, respectively The increase of ionic strength promotes aggregation of thermally denatured actin [5] Aggregation ... were accumulated in the incubation mixture (Fig 4A) These results agree with other data in the literature [6] and indicate that heat-induced unfolding increased the susceptibility of actin to...
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Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx
... the absence of the sHsp This may reflect the formation of very large aggregates of MDH that also include the sHsp, as evidenced by the loss of sHsp from the SEC profile under these conditions At ... light scattering was actually higher than in the absence of the sHsp This may reflect the formation of very large aggregates of MDH that also include the sHsp At low sHsp concentrations the sHsp ... protect MDH at this ratio As the amount of sHsp to substrate is further decreased, most of the MDH is no longer found in complexes, but rather is aggregated and removed by centrifugation prior...
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Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc
... ice and analyzed on the column equilibrated at 25 °C The elution times of peaks from samples run at 25 °C and 43 °C in (B) are indicated (D) Gel filtration calibration curves at 25 °C (m, ––) and ... solid line) The second peak of a sample with a concentration of mgÆmL)1 eluted at % 200 kDa at room temperature Preincubation of the protein at 40 °C did not result in a shift of the peak when ... the major species in the spectrum Representative charge states of the associated oligomers (XmerY+) are shown NanoESI-MS was used to confirm the effects of concentration and temperature upon the...
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Báo cáo khoa học: Mimicking phosphorylation of the small heat-shock protein aB-crystallin recruits the F-box protein FBX4 to nuclear SC35 speckles docx
... ubiquitination of the latter during mitosis, targeting it for degradation We have indeed previously demonstrated that pseudophosphorylated aB-crystallin together with FBX4 promotes the ubiquitination ... percentage of the total aB-crystallin Values are based on four independent experiments and error bars represent the standard error of the mean (SEM) Asterisks indicate the aB-crystallin mutants that ... aB-crystallin S19D/S45D/S59D show the nuclear bodies, indicating that at least part of the detergentinsoluble fraction of the pseudophosphorylated aB-crystallin is localized in these structures Transiently...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... performed at temperatures of 48–60 °C The optimal rate 5890 of precipitation of yeast ADH for monitoring precipitation was found to be 55 °C (not shown), and the inactivation and precipitation of yeast ... phosphate buffer (pH 7.3) containing 0.02% NaN3, with 100 lL being loaded onto the column The peak positions of the elution of molecular standards are indicated at the top of the graph The elution ... fluorescence of wild-type and mutant Hsp25 reached a maximum at a final concentration of 85 lm ANS, and the fluorescence values presented (Fig 3, Table 2) are the means of the plateau region of the ANS...
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Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf
... aggregation at 25 °C, heat-induced citrate synthase aggregation at 43 °C, and maintenance of citrate synthase enzyme activity at 43 °C, WT p26 possessed the greatest chaperone activity and R114A the ... interaction between strands b2 and b6 of neighboring monomers The p26 modifications examined in this study are not located in either of these strands and they are not considered further As the basic p26 ... tetramers, the next level of structure Modeling indicates that tetramer formation depends upon contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands and in the a-crystallin...
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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx
... Ao and At are the intensities of the band of intact protein at the beginning of trypsinolysis and at the fixed time of trypsinolysis) against the time of incubation (Fig 8D) The apparent rate ... dramatically affect the quaternary structure but, at the same time, induce destabilization of the overall structure of the sHsp [35,36] Therefore, we analyzed the effect of point mutations in the ... content of turns and unordered structure (from 58% to 63%) These data might indicate that mutations in the b5–b7 loop and in the N-terminal part of the b7 strands destabilize the structure of HSP22...
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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc
... knockout of Hsf1 and Hsf4, the abnormal levels of FGF-7 returned to normal, and proliferation and differentiation of the epithelial cells were stabilized These findings indicate that HSF1 and HSF4 ... demethylation of histone H3K9 within the binding regions Lack of HSF4 led to increased H3K9 methylation, which is associated with the generation of heterochromatin, and reduced the binding of HSF1 These ... show that HSF4 promotes the DNA-binding activity of another member of the HSF family, through modulating the chromatin status [106] Conclusions and perspectives Nearly 10% of the genes in the human...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... downstream of the initiator ATG in exon (Ex1) of the 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt and the release of an N-terminal fragment bearing the ... ubiquitylated [20] The accumulation of ubiquitylated abnormal proteins results in the formation of pathologic aggregates that perturb the normal physiology of neurons and lead to proteotoxicity The ... observations support the hypothesis that the expression of expanded httEx1 and the presence of httEx1 aggregates ⁄ inclusion bodies correlate with elevated ROS levels, and that NAC and Hsps have different...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... incubated at 30 and 37 °C, but much of the p26 at the higher temperature Fig Schematic representation of p26 cDNAs cloned in pET21(+) Results obtained by cloning full-length and truncated derivatives ... Laboratories) Reaction mixtures, covered with mineral oil, were incubated for at 94 °C prior to five cycles of 30 s at 94 °C, 45 s at 40 °C, 30 s at 72 °C, then 30 cycles of 30 s at 94 °C, 30 s at ... agar and incubated at 37 °C for 20 h Colonies were counted and the log10 values of colony forming units (cfu) per mL were plotted against the length of heat shock in The results shown are the...
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Báo cáo khóa học: Some properties of human small heat shock protein Hsp20 (HspB6) potx
... Rearrangement of the complexes formed by the 3D mutant of Hsp27 and the wild-type Hsp20 A mathematical summation of the elution profiles of the isolated 3D mutant of Hsp27 and isolated wild-type ... or greater than that of commercial a-crystallin At pH 7.0, the heating of isolated ADH in the absence of divalent cations was accompanied by aggregation and a large increase in the light scattering ... unfold and aggregate These aggregates are retarded on the top of the column and therefore not detected on the chromatogram The data presented indicates that acidic pH induced unfolding of Hsp20 In...
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Báo cáo khoa học: Small heat shock protein Hsp27 prevents heat-induced aggregation of F-actin by forming soluble complexes with denatured actin docx
... aggregation in the course of thermal denaturation of F-actin Previous studies have shown that the DLS method allows determination of the size of particles formed in the process of protein aggregation ... during the course of F-actin thermal denaturation All the methods used here show that the size of these complexes depends on the Hsp27-3D ⁄ actin ratio in the initial mixture of Hsp27-3D and F-actin ... together, all these results support a viewpoint that the formation of soluble complexes with non-native proteins is a conserved feature of the sHSP family of chaperones, and the morphology of these...
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Báo cáo khoa học: Characterization of novel sequence motifs within N- and C-terminal extensions of p26, a small heat shock protein from Artemia franciscana potx
... Mutation Primer sequence N-terminal extension G 5¢-GGCACTTAACCCATGGTACATGGACCTTGATATTGAC-3¢ 5¢-GTCAATATCAAGGTCCATGTACCATGGGTTAAGTGCC-3¢ 5¢-GGACCTTGATATTGACGGTCCAGATACC-3¢ 5¢-GGTATCTGGACCGTCAATATCAAGGTCC-3¢ ... with the peak of G and TS at 348 nm and R at 360 nm The fluorescence intensity of R was the lowest and it was red-shifted in comparison to other samples The extent of tertiary structure modification ... p26 prevented heat induced citrate synthase aggregation and chemically induced insulin denaturation at molar ratios of p26 to substrate similar to those of other sHSPs [59–63] The p26 variants...
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Báo cáo Y học: A critical motif for oligomerization and chaperone activity of bacterial a-heat shock proteins pot
... activity and oligomerization [44] In a-crystallin, on the other hand, numerous point mutations and even truncation of the first half of the N-terminal region did not affect complex formation and functionality ... extracts containing truncated His6-tagged HspH variants according to the schematic representation on the right half of the figure After denaturation and renaturation, the extracts were applied ... filtration over a Superdex 200 column at a flow rate of 0.6 mLÆmin)1 N-Terminally truncated derivatives of HspH (A–D) and HspF (E–G) were analyzed and compared with native HspH (H) and HspF (I) The...
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báo cáo khoa học: "Heat-shock proteins in infection-mediated inflammation-induced tumorigenesis" potx
... professional APCs and T-cells, maturation of DCs by upregulating the expression of costimulatory and antigen-presenting molecules such as B7-1, B7-2 and MHC-II molecules, induction of migration of DC to ... patients may enhance the carcinogenic potential of these agents by increasing their rate of activation Furthermore, in patients with S haematobium and bladder cancer, TAMs attracted to the bladder can ... handful of confirmed associations relative to the diversity of human neoplasms and pathogens Nevertheless, the worldwide population burden of infectious organisms makes understanding their role...
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Local induction of heat shock proteins using magnetic fluid hyperthermia for ocular neuroprotection in glaucoma
... c Identification of the induction of HSPs 72 d Investigation of the correlation between the change of AC heating stress and the behavior of HSPs 72 induction e Infusion of SPNPs to the retina ... Understand the physical mechanisms of the AC heating and the crucial physical parameters to improve the AC heating power and SLP f Confirmation of biocompatibility B Development and characterization ... properties, and AC heating characteristics of SPNPs in both solid state and fluid state d Investigation of the physical contribution of Néel and Brown relaxation loss power to the AC heating power of...
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Immunomodulatory roles of heat shock proteins
... after the role of HSPs in antigen presentation has been discovered, emerging data suggest that they are also effective activators of the innate immune system, promoting the maturation of dendritic ... sonication (30 s pulse on, 15 s pulse off, total process time of 10 min) on ice The lysate was spun at 10, 000 x g for 20 at °C and the supernatant was harvested The supernatant was then equilibrated ... make use of the in vitro cell culture system to investigate the role of the HSPs in antigen presentation and signaling Furthermore, we examine the immunogenicity and regulatory properties of HSPs...
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Báo cáo y học: "Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line" pptx
... gene and hypothesized that the heat shock response is negatively modulated by PARP-1 activation in fibroblasts; therefore siRNA mediated PARP-1 inhibition would lead to augmentation of the heat ... cells (Figure 4A and 4B) Collectively, these experiments suggest that PARP-1 negatively modulates the heat shock response i.e knockdown of PARP-1 led to augmentation of the heat shock response ... and incubated on ice for 15 Nuclear proteins were isolated by centrifugation at 14,000 rpm at 4°C for 10 Page of Protein concentrations of the resultant supernatants were determined using the Bradford...
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