assessment of suppressive capacity by human regulatory t cells using a reproducible bi directional cfse based in vitro assay

Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot

Ngày tải lên : 09/08/2014, 03:24

... determine the differentiation Available online http://arthritis-research.com/content/4/4/241 pathway that a naive T cell will take following activation These include the antigen concentration and ... possible to generate sufficient numbers in humans for clinical trials Although this will be carried out initially with mitogens as the T- cell activating agent, the ultimate goal is to induce autoantigen-specific ... in this fraction is the generation of ‘professional’ regulatory T cells remains to be determined Our finding that both IL-2 and TGF-β are critical in the generation of regulatory T cells is of...

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Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf

Ngày tải lên : 14/08/2014, 16:21

... CAT-3' TNFRSF1B 5'-GTA GCC TTG CCC GGA TTC TGG-3' 5'-ACC CTG CCC CTG CTC TGC TA-3' TRAF1 5'-GGG GCA TAA ACT TTC CTC TTC C-3' 5'-TTT GGG GTT ATA CAT TGC TCA GTG-3' LGALS3 5'-CCT TTG CCT GGG GGA ... downregulation of FHIT also contributes to the inactive status of TP53 Based on our data, we suggest that destabilization and thereby inactivation of TP53 provokes a shift in TReg cells from apoptotic ... directed by the transcription factors T- bet and GATA3, respectively STAT4 and STAT6 were both downregulated in the peripheral TReg cells, indicating a potential inability to be transformed into Th cells...

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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps

Ngày tải lên : 09/08/2014, 01:23

... fraught with complications and an occasional lack of certitude regarding their undeniable existence as a functionally distinct population rather than simply another activation state of conventional ... CD25+CD4+ cells after stimulation through the TCR and might therefore mediate its effects in a membraneproximal manner [53] The level at which these anti-inflammatory cytokines operate to maintain tolerance ... by the TR cells acts as an interleukin-2 sink and hinders the autocrine/paracrine stimulation of TE cells 23 Arthritis Research & Therapy Vol No Fehérvári and Sakaguchi ably an as yet uncharacterised...

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Báo cáo y học: "Apoptotic cell-mediated suppression of streptococcal cell wall-induced arthritis is associated with alteration of macrophage function and local regulatory T-cell increase: a potential cell-based therapy" ppsx

Ngày tải lên : 09/08/2014, 14:22

... reduction of circulating TGFβ in SCWinduced inflammation at day and the slight increase in circulating active TGFβ at day in apoptotic cell-treated animals at the time of articular index reduction ... a specific pathogen-free rodent facility at the National Institute of Dental and Craniofacial Research, National Institutes of Health All animal studies were performed according to National Institutes ... PBS (Biowhittaker) containing 1% BSA (Irvine, Santa Ana, CA, USA) For surface staining, cells were incubated with FITC-conjugated anti-rat CD4 (Caltag, San Francisco, CA, USA) and allophycoyanin-conjugated...

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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx

Ngày tải lên : 18/06/2014, 15:20

... supernatant inhibit the maturation of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric assay of soluble class ... ability of vaccination with autologous FCs to stimulate T cells The ability of vaccination with autologous FCs to stimulate T cells A, Nonadherent PBMCs obtained before and after vaccination were stimulated ... capacities in vitro Treg may contribute to the attenuated CTL responses in the presence of the supernatants A major obstacle to the development of any active immunotherapeutic approach to cancer...

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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps

Ngày tải lên : 12/08/2014, 13:22

... almost CD127 negative, indicating that these T cells were Tregs The most important finding in the present study was that CD39+Tregs could be able to inhibit the generation and differentiation of Th17 ... determined by flow cytometry after surface staining or intracellular staining with anti -human- specific Abs conjugated with either phycoerythrin or fluorescein isothiocyanate These human Abs included ... responses in vivo in a signal transducer and activator of transcription-3-dependent manner, and Treg cell-specific ablation of signal transducer and activator of transcription-3 leads to the loss of their...

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Ecological Assessment of Water Quality by Three-species Acute Toxicity Test and GC/MS Analysis - A Case Study of Agricultural Drains

Ngày tải lên : 05/09/2013, 10:15

... of the ecotoxicity test solution that is made from a concentrated water sample The growth inhibition ratio for algae, immobilization (swimming inhibition) ratio for daphnia and mortality ratio ... exceed in the long term means that it is an undesirable status for the inhabitation of aquatic organisms The results of the measurement and the calculation of DVi, DIi and DI are shown in Table Three ... ratio for daphnia and mortality ratio for fish in each acute toxicity test were used as water quality indexes Of the monitoring sites evaluated in this study, 38% showed good water quality based...

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Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

Ngày tải lên : 07/03/2014, 21:20

... 5¢-TTGCAACTGCTGTGTTCACTGTTC-3¢, 369 bp; human ODC, 5¢-TGTTGCTGCTGCCTCTACGTT-3¢ and 5¢-GCTGGCATCCTGTTCCTCTACTT-3¢, 138 bp [51]; human b-actin, 5¢-ACAATGAGCTGCTGGTGGCT-3¢ and 5¢-GATGGGCACAGTGTGGGTGA-3¢; ... murine b-actin, 5¢-TGGAATCCTGTGGCATCCATGAAAC-3¢ and 5¢-TA AAACGCAGCTCAGTAACCGTCCG-3¢ Human GAPDH primers were included in the Advantage RT-PCR kit Immunoblot analysis Experimental procedures Reagents ... the development of the antiviral state IRF-3-mediated antiviral response involves spermine TCACACGTGCTTGATT-3¢ [50], 362 bp; human and murine ArgI, 5¢-ATTGGCTTGAGAGACGTGGACCCT-3¢ and 5¢-TTGCAACTGCTGTGTTCACTGTTC-3¢,...

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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

Ngày tải lên : 16/03/2014, 12:20

... results obtained indicate that both the substrate speciﬁcity and catalytic efﬁciency of human arginase I are markedly altered by replacement of Asn130 with aspartate In addition to supporting the ... introducing a glutamine residue at this position In fact, the N130Q variant was found to be totally inactive on agmatine, although it retained about 60% of the wild-type arginase activity and exhibited ... compilation ª 2006 FEBS 5627 ´ R Alarcon et al Agmatinase activity of the N130D mutant arginase I Fig Effects of dialysis against EDTA on the catalytic activity of wild-type and N130D variants of human...

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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt

Ngày tải lên : 17/03/2014, 03:20

... host range of JCV replication in vivo [52] Alternatively, rodent, but not human, chromatin might contain a nondiffusable factor that inhibits JCV TAgdependent DNA replication or the phosphorylation ... signiﬁcant activity in the DNA replication assay In both the SV40 and JCV assays, incorporation of nucleotides is dependent upon an intact origin of replication; either plasmids carrying the noncognate ... required to facilitate initiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting in origin unwinding under conditions of altered template DNA superhelicity...

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Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx

Ngày tải lên : 31/03/2014, 07:20

... catalytic capacity for GSNO reduction with NADH as coenzyme Mutational analysis demonstrates that GSNO binds in a similar manner to HMGSH to ADH3 As the major products formed are GSSG and glutathione ... GSSG are indicated by arrows (B) MS of the fraction corresponding to peak in (A) demonstrating the identity and purity of the glutathione sulﬁnamide Notably, no contaminants of GSSG, GSNO, NAD+ ... However, for the bacterial ADH3, Liu et al not report any detection of glutathione sulﬁnamide but propose that the intermediate semimercaptale reacts with NADH to form glutathione amine, which...

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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot

Ngày tải lên : 18/06/2014, 15:20

... response and the generation of tolerance to TAAgs In contrast, partially mature but activated DCs are optimal for antigen-loading strategies that require internalization and cell processing CD83 ... for patients with prostatic cancer and bony metastasis BJU Int 2002, 89:710-713 Stamey TA, Kabalin JN: Prostate specific antigen in the diagnosis and treatment of adenocarcinoma of the prostate ... Cytotoxicity of In Vivo Generated T Cells T2 -cytotoxicity Cumulative cytotoxicity results for all patient samples show that after two cycles of vaccination (the time point associated with the...

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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx

Ngày tải lên : 08/08/2014, 21:20

... routes with ease of applicability that will have persistent clinical effectiveness that can be built within a short duration of therapy time.1 107 Early and Late Effects of SIT on Mast Cells, Basophils, ... several hours, which supports the effect of SIT on early desensitization It has been demonstrated that an absolute amount of histamine released in response to stimulation was decreased after major ... immediate-phase and late-phase allergic inflammation SIT also reduces the number of effector cells and release of their mediators in the target tissues It has a role in cellular and humoral responses...

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Báo cáo y học: " Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen" pptx

Ngày tải lên : 09/08/2014, 01:23

... Discussion Treatment of autoimmune diseases by induction of oral tolerance is attractive because of the few side effects and easy clinical implementation of this approach Although protein antigens administered ... hour at room temperature with biotinconjugated rat antimurine IL-10 and TGF-β monoclonal antibodies, followed by an alkaline phosphataseconjugated goat antibiotin monoclonal antibody (R&D Systems) ... San Jose, CA, USA) Statistical analysis Experimental findings are presented as mean ± standard deviation Statistical significance was determined by Student’s t- test using the SPSS program (version...

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Báo cáo y học: "CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease" potx

Ngày tải lên : 09/08/2014, 01:23

... expression, an enrichment of regulatory T cells was seen Thus, the simple extrapolation of the animal data into future therapeutic strategies that aim at reconstituting this population does not seem to ... spondyloarthropathies Excluded are patients with reactive arthritis and gastrointestinal inflammation Table Cytokine production and proliferation of CD25- CD4+ T cells in in vitro cultures Patient ... local, intra-articular, treatments that the patients were receiving Only those patients with documentation of intra-articular cortisone injection within months before sampling are depicted in...

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Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx

Ngày tải lên : 09/08/2014, 01:24

... patients with RA R549 We demonstrated that anti-CII monoclonal antibodies are capable of initiating arthritis independently of B and T cells during the effector phase of arthritis Already activated ... CII-specific T cells could have a role in the perpetuation and exacerbation of already established disease rather than having any direct influence on the initiation phase of arthritis Competing interests ... autoreactive T cells could have a different effect on the acute phase of arthritis However, T cells again did not affect the initiation phase of the disease; instead, the effect was noted in the...

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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot

Ngày tải lên : 09/08/2014, 01:24

... of differentiating into TR cells following activation are derived from a separate lineage of CD4+ T cells, and whether their induction is a result of the initial activation early in the inflammatory ... death, TR cells lead to the induction of T regulator (Tr1) cells and Th3 cells, which feed back to inhibit inflammation, and the TR cells inhibit proliferation of antigen-specific and bystander ... autoreactive T cells that have escaped into the periphery Regulation of autoreactivity is present at the level of thymic selection, but also in the periphery Those autoreactive cells that escape negative...

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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc

Ngày tải lên : 09/08/2014, 06:22

... much as cells from mice treated with control IgG These data imply that a substantial proportion of the T- cell compartment is still activated 14 days after intra-articular antigen challenge in the ... into antigen-induced arthritis (AIA) mice at day After 24 hours radioactivity in isolated organs and the rest of the body was determined using a γ-counter Thereafter, the total radioactivity ... effective in preventing AIA [10], had no effect on disease at this time point (Fig 5c) Taken together, our data demonstrate that Treg cells can inhibit arthritis development when transferred at the...

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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx

Ngày tải lên : 09/08/2014, 06:23

... benchmark therapy for RA, drastically influences the function of CD4+CD25+ Treg [18] The crucial point of that study was that Treg isolated from patients with active RA before treatment with anti-TNF-α ... shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of fully functional CD4+CD25+ Treg, RA is still not cured These strands of evidence ... suppressor activity, RA is not suppressed but the disease is instead very active The authors provided a startling explanation for this apparent incongruence, in their discovery that tissue-infiltrating...

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Báo cáo y học: "The contact-mediated response of peripheral-blood monocytes to preactivated T cells is suppressed by serum factors in rheumatoid arthritis" ppt

Ngày tải lên : 09/08/2014, 07:20

... by using a commercially available test kit (N antisera against human ApoA-1; Dade Behring, Liederbach, Germany) Statistical analysis For statistical analysis, the software package Sigma Stat (SPSS ... this interaction in the breakdown of extracellular matrix and subsequent joint destruction in RA To investigate cell-contact-mediated activation of monocytes by preactivated T cells, monocytic ... strong inhibitory activity of RA sera cannot be explained by an increased ApoA1 concentration alone, although the result of a significant correlation between ApoA-1 concentration and inhibitory...

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