... rat Caskin1 cDNA was kindly provided by
Thomas Su
ă
dhof (University of Texas Southwestern Medical
Center, Dallas, TX, USA), and the full-length Abi2 cDNA
was donated by Ann Marie Pendergast (Duke ... as far as the measured
NMR parameters were concerned. The temperature was
controlled by an open-cycle Oxford cryostat with a stability
of ± 0.1 °C; the uncertainty of the temperature scale was
±1°C.
1
H-NMR ... spectroscope on sample change was controlled
by measuring the length of the p ⁄ 2 pulse to obtain reliable
M
0
values [55]. The extrapolation to zero time was
performed by fitting a stretched exponential.
V....
... fraction
(LV-EF) was calculated by the modified Simpson´s
method. LA and LV diameter were measured by
M-Mode echocardiography. High grade cardiac val-
vular disease was excluded by Color, PW and ... strain
rate measurements by cardiac ultrasound: principles, imple-
mentation and limitations. Eur J Echocardiogr 2000; 1(3):
154-170.
3. Mirsky I, Parmley WW. Assessment of passive elastic stiffness ... myocardium (see Table 3).
Table 3: Tissue velocities (S´, E´, A´) of basal, mid and
apical segments as assessed by VVI
Basal Mid Apical p ANOVA
S´ (cm/s) 3.64 ± 1.63 2.41 ± 1.07 1.06 ± 0.65...
... task-based teaching and task-
supported teaching. The task-based teaching occurs when the teaching is based exclusively on
meaning-focus tasks, and the task-supported teaching occurs when tasks ... can significantly increase total of time- on-task, which
is useful for pupils in the class and reduce maximum the waste time. Comparing total of time,
which were used by pupils to participate ... (not on task, not misbehaving). At the end of the
observation session, the data are tallied and a percent time- on-task score is assessed. In order to
19
accurately assess time- on-task, the...
...
parent commits: giving the baby a name. As any modern parent
knows, the baby-naming industry is booming, as evidenced by a pro-
liferation of books, websites, and baby-name consultants. Many par-
ents ... black
woman who was upset with the name just given to her baby niece. It
was pronounced shuh-TEED but was in fact spelled “Shithead.” *
Shithead has yet to catch on among the masses, but other ... there was a great overlap between black
and white names. The typical baby girl born in a black neighborhood
in 1970 was given a name that was twice as common among blacks
than whites. By 1980...
... BindingManagerBase object by 1.
Move Next Button.Click
Sets the current record of the bound controls to the next record by incrementing
the Position property of the BindingManagerBase object by 1. ...
Move Last Button.Click
Sets the current record of the bound controls to the last record by setting the
Position property of the BindingManagerBase object to the total number of
records, as returned ... database.
conn.Open( );
Byte[] image = (Byte[])cmd.ExecuteScalar( );
// Write to a stream removing the image header.
Sets the current record of the bound controls to the previous record by...
... classification, we end up with 89 flights representative
of night and early morning observations as well as 73 flights representative of daytime
observations, from 16 July to 31 August 2003. In time ... tagging a tropospher ic particle that has got a high PV value by
diabatic PV production in cloudy areas as a stratospheric-origin particle. Once a parti-
cle has gone across a boundary limit of the ... triggered
by forest fires may be able to inject aerosol into the stratosphere at high latitudes. In
the present case, when progressing from Portugal to United Kingdom, the weak extra-
tropical low was associated...
... that loop
flexibility must be responsible for the endopeptidase
activity of cathepsin B, as well as that endopeptidase
activity should be associated with the occluding loop
displacement from the ... are
shifted. As seen in the tetragonal form of the cathepsin
B chagasin complex (3CBK), the depth of the binding
of inhibitor as well as the shift of the occluding loop
can be additionally extended by ... Cathepsin B differs from other cathep-
sins by its dual role, exhibiting exo- as well as endo-
peptidase activity. The crystal structure of this human
enzyme [11] has revealed that an 20 residues long
insertion,...
... such as Asp12, Glu50, Asp72 and Glu133,
are clustered in the vicinity of Asp136. It has
been reported that the corresponding mutation
(Asp134 fi His) stabilizes Ec-RNase HI by 7.0 °Cin
T
m
as a ... thermophilus RNase HI (Tt-RNase HI).
Gly39 and Gly97 are conserved as Gly41 and Gly100
in Tt-RNase HI, respectively. Val76 is conserved as
Val74 in Ec-RNase HI. Asn90 is conserved as Gln96
in RNase HI ... R97G-RNase HI, D136H-RNase HI,
5·-RNase HI and 6·-RNase HI were constructed by site-
directed mutagenesis using PCR as described previously
[33]. Plasmid pET500M or pET500M4x was used as tem-
plate....
... thioredoxin
fusion by cleavage at the enterokinase site provided by
the vector was unsuccessful. However, the thrombin
site, located 30 amino acids upstream of the native
synthetase sequence, was accessible ... the
native protein) in the soybean arginyl-tRNA synthe-
tase was not targeted by this protease. The position of
the cleavage was confirmed by N-terminal protein
sequencing. The results reported ... amplicon was sequenced using
the M13 primer to give the Canavalia ensiformis 3Â-terminal
55-base sequence. The remaining 5Â region was assembled
taking into account conserved D-loop bases and the
base-pairing...