aqueous reactions and nmr spectroscopy of hydroxamidovanadate
Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... structures were examined using CD spectroscopy and 2D homonuclear/heteronuclear NMR spectroscopy Using these peptides, the interaction between the amphipathic domain of YopD and its cognate chaperone ... the addition of small aliquots of HCl and NaOH NMR Ó FEBS 2002 Tertiary structure of the YopD amphipathic domain (Eur J Biochem 269) 3661 experiments were also conducted between 10 and 50 °C to ... [35] and VMD [36] In order to verify that no residues were in disallowed regions, Ramachandran plot analysis was conducted using the program PROCHECK -NMR [37] RESULTS AND DISCUSSION CD and NMR...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... different techniques, of which MS and NMR are two of the most powerful Previously, we demonstrated the distinction of chain type and blood-group type (such as Lea/x and Leb/y) of underivatized oligosaccharides ... this report, we demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosaccharides isolated from human ... order to understand post-translational glycosylation and its function Expansion of our knowledge on the repertoire of carbohydrate structures, the ÔglycomeÕ, and investigation of oligosaccharide...
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Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx
... transport of lipophilic compounds and drugs and of medium and long chain fatty acids; among them, myristic acid is a stereotypic ligand to investigate fatty acid binding and transport properties of ... report the spectroscopic analysis of the myristate-dependent conformational changes of the N and B states of Mn(III)heme–HSA, by optical absorbance spectroscopy and NMR spectroscopy, that show allosteric ... Between pH 4.3 and 8, in the presence of allosteric effectors, and at pH greater than 8, in the absence of ligands, HSA changes conformation to the basic (B) form with loss of a-helix and an increased...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... A-chain and B-chain of nPS, and in the case of His37, the v1 torsion angle changes from 63.34° to )67.80° and )52.11° for the A-chain and B-chain of nPS, respectively The imidazole ring of His34 ... probe All NMR spectra were acquired at 303 K NMR data were processed using nmrpipe ⁄ nmrdraw [53], and assigned using ansig [54,55] The NMR and MS facilities at the Indian Institute of Science ... structures of PSs from E coli and M tuberculosis Structural analyses Analysis of the sites of substrate binding from NMR data, and structural comparison of the E coli, M tuberculosis and A thaliana...
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Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... enzyme reactions were stopped with addition of 10 lL 10% aqueous trifluoroacetic acid and immediately injected onto RP-HPLC and fractions collected were collected and analyzed with ESI and MALDI-MS ... increments in t1 and spectral widths of 8000 Hz and 17 591 Hz in the 1H and 13C dimensions, respectively A total of 256 summed scans were collected with a relaxation delay of 1.3 s All 1H and 1H-13C ... between GNH3 of GalNAc and GH1 of Gal, which is labeled in bold The amino acids Gly6, Thr10 and Pro13 are represented by 6G, 10T and 13P Ó FEBS 2004 Glycopeptide tx5a synthesis and NMR analysis...
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Báo cáo hóa học: " FTIR and Raman Spectroscopy of Carbon Nanoparticles in SiO2, ZnO and NiO Matrices" pdf
... optical mode of the out of phase mode of the asymmetric vibration or to the longitudinal optical mode of the high frequency vibration of SiO2 [8] On the other side of the major absorption band, at ... (X-HRTEM), and selected area electron diffraction (SAED) Results and Discussion FTIR Spectroscopy Figure 1a shows the variation of total reflectance with the amount of carbon precursor (SUC) of C–SiO2 ... are weaker than the G peaks and this suggests that the grain sizes could be larger than 44 nm [12] From the peak positions xD and xG and intensities ID and IG of the D and G-peaks, respectively,...
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Chemical of alumina reactions in aqueous solution and its application in water treatment
... of these groups to bind protons and adions, and the bonding requirements of protons and adions The molecular structures and compositions of surface functional groups and adion complexes are of ... thermodynamic, mechanistic and kinetic description of surface reactions w3x Because of all the above reasons, the structure and composition of surface groups and reactions with organic and inorganic compounds ... three planes of charge: O-, b- and d-plane are associated with three planes of potential C0, Cb, and Cd and treated as a series of pairs of parallel-plate capacitors with capacitances C1 and C2 w37,87...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... analysis by 1D NMR spectroscopy Molecular alterations of the a-PEC peptide demonstrated by NMR spectroscopy The purpose of the NMR study was not the detailed structural analysis of the two chromopeptide ... presents the purification and molecular analyses of photoactive a-PEC peptides from phycobilisomes of M laminosus Preliminary results of crystallization and NMR spectroscopy offer reliable information ... p.p.m and 9.38 p.p.m., can be attributed to the Z- and E-states, respectively Integration of both pairs of peaks yields the ratios of state populations of Z/E ¼ 12%/88% for the E-state and approximately...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
... single-quantum NMR spectroscopy of aqueous solutions J Biomol NMR 2, 661–665 27 Wuthrich, K (1986) NMR of Proteins and Nucleic Acids John Wiley & Sons, New York 28 Davis, D.G & Bax, A (1985) Assignment of ... studies of the interaction of antimicrobial peptide and lipid bilayers Biochem Biophys Acta 1462, 185–200 Epand, R.M & Vogel, H.J (1999) Diversity of antimicrobial peptides and their mechanism of ... peptide and membranes was obtained upon examination of the NMR- calculated average peptide conformations, displaying the relative positions of the two central lysine residues (Lys-5 and Lys-9) and...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
... difference between the EM2 and EM2OH was observed for the pH dependence of their NMR spectra Characteristically, the NMR spectra of EM2 in water of pH 2.7 and DPC micelles of pH 3.5 were the same ... characteristics of EM2 and EM2OH in DMSO, TFE, H2O (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2) Open and fold represent the conformations rS and n7 in parentheses indicate the reverse S- and numerical ... the solution conformations of EM2 and EM2OH in trifluoroethanol (TFE), water (pH 2.7 and 5.2) and aqueous dodecylphosphocholine (DPC) micelles (pH 3.5 and 5.2); some of the results have been reported...
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Structural and equilibrium unfolding studies of sam domain of DLC1 by NMR spectroscopy
... AND EQUILIBRIUM UNFOLDING STUDIES OF SAM DOMAIN OF DLC1 BY NMR SPECTROSCOPY THESIS BY YANG SHUAI SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF ... acquisition and analysis CHAPTER RESULTS AND DISCUSSION 3.1 Expression and purification of DLC1-SAM 3.1.1 Expression and purification of SAM60 3.1.2 Expression and purification of SAM76 3.2 NMR resonance ... labeling of recombinant proteins have drastically expanded the application of NMR spectroscopy NMR spectroscopy is now not only one of the most powerful techniques for providing atomic details of proteins,...
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Mapping of the binding surface between EPHA5 and antagonist peptide by NMR spectroscopy
... study of these Eph/ephrins will help us to understand the detailed mechanism of the binding and recognition between Eph receptors and ephrin ligands The structures of the ligand-binding domains of ... surface of EphA2 (Figures 4) Eph receptor strands D, E and J, define the two sides of the channel, whereas strands G and M line its back The ligand binds by approximating the side of its β-sandwich ... crystallography, NMR spectroscopy, and cyro-electron microscopy Both X-ray crystallography and NMR spectroscopy can yield information at 11 atomic resolution 1.2.1 Introduction to NMR spectroscopy 1.2.1.1 NMR...
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35768 Study of Electrode Reactions and Interfacial Properties
... extracellular environment of the brain The good temporal and chemical resolutions of such in vivo cyclic voltammetric experiments offer improved understanding of the chemistry of the brain These repetitive ... absorptivity of R and DO and CO are the diffusion coefficient and concentration of O, respectively Hence, A increases linearly with the square root of time (t1/2), reflecting the continuous generation of ... background contributions and holds considerable promise for mechanism diagnosis and kinetic characterization of coupled chemical reactions 46 STUDY OF ELECTRODE REACTIONS AND INTERFACIAL PROPERTIES...
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Bài soạn Chapter 4 Chemical Quantities and Aqueous Reactions
... moles of each of the substances involved in the reaction C8H18(l) + 25 O2(g) → 16 CO2(g) + 18 H2O(g) molecules of C8H18 react with 25 molecules of O2 to form 16 molecules of CO2 and 18 molecules of ... O2 to form 16 molecules of CO2 and 18 molecules of H2O moles of C8H18 react with 25 moles of O2 to form 16 moles of CO2 and 18 moles of H2O mol C8H18 : 25 mol O2 : 16 mol CO2 : 18 mol H2O Chemistry, ... combustion of 9.0 g of glucose? C6H12O6(s) + O2(g) → CO2(g) + H2O(l) convert 9.0 g of glucose into moles (MM 180) convert moles of glucose into moles of water convert moles of water into grams...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... of a hinge point at Arg53 that exhibits conspicuous minima of J(0) and J(xN) and a significant maximum of J(0.87xH), and that emerges not only within helix III, but also over a large portion of ... residues and hence does not conflict with the global interpretation of the data With the exclusion of the N-terminal octapeptidyl and C-terminal nonapeptidyl fragments of Glu17, Leu34 and Lys46, ... dynamics of the rat TTF-1 homeodomain Table Mean values and corresponding standard deviations (in parentheses) of S2 (dimensionless) and se (ps) parameters for the secondary structure elements of the...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... variety of methods, e.g trNOEs [20], STD NMR [21–23], and SAR by NMR [24] TrNOE spectra can also be used to elucidate the 3D structure of the bound ligand Saturation transfer difference (STD) NMR ... intraresidue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference ... resonances of Pro1 and Asp2 have the highest intensities in the STD spectrum, signals of Thr3 are of medium intensity, while the signals of Arg4 and Pro5 have the lowest intensity The d-protons of Pro5...
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Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt
... NMR spectrum of WNV NS2B–NS3pro (K96A) Fig S3 15N-HSQC spectra of combinatorially 15Nlabelled samples of WNV NS2B–NS3pro in the presence of Fig S4 800 MHz 1D 1H NMR spectra of the compounds and ... exception of prolines and a few residues with very broad amide peaks The resonances of the peptide connecting NS2B and NS3pro appeared at chemical shifts characteristic of random coil conformation and ... site A NOESY spectrum of and in the presence of a small quantity of protease revealed an intermolecular cross-peak between the methyl group of and the phthalazine ring of 2, as expected for an...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... observation and dynamics of specific amide protons in T4 lysozyme Biochemistry 24, 817–822 McIntosh LP & Dahlquist FW (1990) Biosynthetic incorporation of 15N and 13C for assignment and interpretation of ... resonance assignments of the [15N]-HSQC spectrum, where it is often difficult to assess the magnitude of chemical shift changes upon ligand binding in [15N]-HSQC spectra of uniformly labelled...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
... 2002 Fig 1D 1H NMR (A) and STD NMR (B and C) spectra of long-chain PS from strain RC1 in the presence (A and B) and absence (C) of mAb 2625 Rather strong unspeci®c irradiation of ligand signals ... to Fig Fig 1D 1H NMR (A and C) and STD NMR (B and D) spectra of long-chain PS from strain RC1 in the presence of mAb 2625 recorded with p2H 7.4 at 293 K (A and B) and 315 K (C and D) Only the ... 269) Ó FEBS 2002 Fig 1D 1H NMR (A and C) and STD NMR (B and D) spectra of middle-chain PSNH4OH/HF from wild-type RC1 (A an B) and mutant 5215 (C and D) in the presence of mAb 2625 Low intensity...
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The Credit Crunch of 2007-2008: A Discussion of the Background, Market Reactions, and Policy Responses potx
... characteristics: The Board of Governors of the Federal Reserve System, the Office of the Comptroller of the Currency, the Federal Deposit Insurance Corporation, and the Office of Thrift Supervision ... scope of regulation necessary for ratings agencies, and review the usefulness of stress testing and “fair value” accounting methods This article consists of two parts: an outline of events and ... spread most often used by central banks to describe the increase in the cost of interbank lending, reflecting credit and liquidity risk See Arain and Song (2008, p 2) and Bank of England (2008,...
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