aptamers from discovery to practice

nanotechnology for cancer therapy, 2007, p.810

Ngày tải lên : 04/06/2014, 14:30

... cytotoxic agent back to the systemic circulation (a macrophage depot system), as well as induction of Kupffer cell apoptosis.10 Following apoptosis, restoration of Kupffer cells may take up to ... targeted delivery to breast cancer cells to improve the efﬁcacy to safety quotient relative to direct administration of this cytotoxic agent.60 Coupling of doxorubicin-loaded liposomes to antibodies ... tumors Secretory phospholiapse A2 not only acts as a trigger resulting in the release of encapsulated cytotoxic drugs from pro-drug ether liposomes, but also generates highly cytotoxic lysolipids...

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INHIBITION OF APE1’S DNA REPAIR ACTIVITY AS A TARGET IN CANCER: IDENTIFICATION OF NOVEL SMALL MOLECULES THAT HAVE TRANSLATIONAL POTENTIAL FOR MOLECULARLY TARGETED CANCER THERAPY

Ngày tải lên : 24/08/2014, 13:10

... chemotherapeutic agents used to treat gliobastomas induce lesions repaired by the BER pathway AR03 is cytotoxic to SF767 glioblastoma cancer cells as a single agent and enhances the cytotoxicity of alkylating ... important to maintain these transcription factors in a reduced state prior to their transport to the nucleus (44) Ape1 has also been shown to accumulate in the nucleus and mitochondria in response to ... an inhibitor of the BER pathway such as methoxyamine (MX) (125) leads to an accumulation of AP sites Failure to repair such accumulated AP sites leads to cytotoxicity, increased apoptosis 25...

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Báo cáo sinh học: " Encapsulation of docetaxel in oily core polyester nanocapsule intended for breast cancer therapy" pptx

Ngày tải lên : 18/06/2014, 22:20

... evaluation of Doc-loaded nanocapsules' cytotoxicity The NCs' cytotoxicity was evaluated using the SUM 225 cell line (Asterand, Inc., Detroit, MI, USA) The cytotoxicity after treatment of these cells ... led to a high EE% (see pairwise comparison: F1 to F13, F3 to F9, F4 to F11, F5 to F10, respectively) At a low PLA content, the EE% was decreasing regardless of the oil content (comparing F9 to ... ultramicrotome (Leica Microsystems Ltd., Milton Keynes, UK) with a Diatome diamond knife (Diatome, Hatfield, PA, USA) The sections were collected on 200-mesh copper grids and put into the TEM...

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báo cáo hóa học:" PTTG: an important target gene for ovarian cancer therapy" doc

Ngày tải lên : 20/06/2014, 07:20

... Ramos-Morales F, Dominguez A, Romero F, Luna R, Multon MC, Pintor-Toro JA, Tortolero M: Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene product Oncogene 2000, 19:403-409 ... may contribute in part to PTTG1's regulatory effect In addition to ID3, Kim et al [72] also showed that the angiogenic inhibitor TSP-1 is decreased by 2.5-fold in response to PTTG1 overexpression ... angiogenesis and metastasis D Apoptosis PTTG1 has been shown to regulate the expression of p53, a tumor suppressor gene that subjects to apoptosis by inducing apoptosis-inducing genes Yu et al [26]...

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báo cáo khoa học: " Polymeric nanoparticle-encapsulated curcumin ("nanocurcumin"): a novel strategy for human cancer therapy" ppsx

Ngày tải lên : 11/08/2014, 00:22

... cancer lines in the laboratory (selected reviews include [1,4-6,40,41]) Equally important, free curcumin was shown not to be cytotoxic to normal cells, including hepatocytes, mammary epithelial ... attributable to the saturated protons of the polymeric network The broad resonance peak at δ = 0.8–1.0 ppm are from the methyl protons of the isopropyl group The signal peaks for the methyne proton (>CH-) ... pro-inflammatory cytokines Nanocurcumin downregulates steady state transcripts of multiple pro-inflammatory cytokines Peripheral blood mononuclear cells (PBMCs) were activated with phytohaemagglutinin...

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báo cáo khoa học: "Free Rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy" docx

Ngày tải lên : 11/08/2014, 00:23

... (H2cit)4 has significant antitumor, cytotoxic, and cytostatic activity on Ehrlich ascites tumor [7] Although toxic to normal cells, its lower toxicity when compared to carboxylate analogues of rhodium ... because they showed toxicity in normal cells [4,31] In our study, Rh2(H2cit)4 was also cytotoxic to in vitro normal cells The IC50 values (Table 2) showed that Rh (H2cit)4 cytotoxic effect was more ... concentrations These effects were related to several structural and morphological alterations, probably coming from cell death by apoptosis and autophagy Further, higher cytotoxicity in the MCF-10A breast...

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Delivery of proapoptotic biomolecules and drugs using amphiphilic block copolymer nanoparticles for anti cancer therapy

Ngày tải lên : 30/09/2015, 05:59

... with topotecan, which were found to have highest effectiveness when cells were exposed to the alkylating agent or platinating agent prior to topotecan treatment [68] Another important factor is ... line) Cytotoxicity studies have shown that the co-delivery system synergistically enhances cytotoxic and anti-proliferative effects in both wild type and TRAIL-resistant SW480 cells Receptor-blocking ... at levels at or close to the maximum tolerated dose (MTD) (Box 1.1) to achieve optimal therapeutic effects with tolerable side-effects Therefore, there is a limitation as to the amount of single...

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báo cáo hóa học:" Development of targeted therapy for ovarian cancer mediated by a plasmid expressing diphtheria toxin under the control of H19 regulatory sequences" doc

Ngày tải lên : 18/06/2014, 15:20

... tumor-selective expression of a toxin, delivered by a non-viral vector Non-viral vectors appear promising due to their potential to overcome the main disadvantage of adenoviral vectors, causing immune responses ... samples from the peritoneum of patients suffering from ovarian cancer were submitted to this study following approval of the Israeli Ethics Committee Samples were kindly given to us from the ... unintended toxicity to normal cells, since the DT-A protein released from the lysed cells is not able to enter neighboring cells in the absence of the DT-B fragment [29] In order to determine...

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Báo cáo hóa học: " Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2-P4 regulatory sequences" potx

Ngày tải lên : 18/06/2014, 16:20

... promoter toxin vector in heterotopic and orthotopic mouse models Heterotopic bladder cancer model was used to evaluate tumor growth inhibition of the double promoter vectors compared to that of ... phenomenon of IGF2 and H19 This vector was then used to transfect and to eradicate tumor cells in culture or to inhibit tumor growth (in vivo), in heterotopic and orthotopic bladder tumor models The ... (containing T7 and Sp6 promoters) were purified from the gel, sequenced and found to be identical to the relevant published sequences in the gene bank 100 to 200 ng from the purified products were used...

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Improvement of different vaccine delivery systems for cancer therapy pptx

Ngày tải lên : 06/03/2014, 02:21

... effectively and directly transferred into the cytosol This system has been applied to deliver proteins into cells [24], but not yet to deliver antigens into DCs for cancer immunotherapy In vitro ... polypeptides will recognize and bind to cell surface receptors that are unique to target cells and deliver the bound DNA into the cells through receptormediated endocytosis Therefore, this process may ... compared to needle intramuscular and biojector administrations in mice model [20] Ultrasound Ultrasound (US) can be used to transiently disrupt cell membranes to enable the incorporation of DNA into...

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Báo cáo khoa học: Hyaluronan–CD44 interactions as potential targets for cancer therapy pptx

Ngày tải lên : 28/03/2014, 23:20

... or hepatocyte growth factor (HGF) [22,23] The rather short cytoplasmic tail of CD44 binds to ankyrin and ezrin–radixin–moesin proteins, providing a link to the cytoskeleton, as well as to merlin, ... hematopoietic cells CD44, in addition to its function to regulate cell proliferation ⁄ differentiation ⁄ survival ⁄ migration into tissues, is implicated in hematopoietic progenitor trafﬁcking to ... from the fact that tumors, similar to wounds, produce chemoattractants, such as cytokines ⁄ chemokines (e.g vascular endothelial growth factor, transforming growth factorb), to recruit MSCs to...

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recombinant antibodies for cancer therapy, methods and protocols

Ngày tải lên : 11/04/2014, 10:25

... candidates for cytotoxic chemotherapy (44) To retarget effector cells of the immune system, bispecific chimeric or humanized antibody molecules have been developed to activate cytotoxic T cells ... since they are unable to mediate natural effector functions owing to the lack of the Fc portion of whole antibodies This was accomplished by linking them to toxins (60–64) cytotoxic ribonucleases ... used to select antigen-specific antibodies from libraries made from human B cells taken from individuals either immunized with antigen (60), or exposed to infectious agents (61), or with autoimmune...

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nanocarriers as an emerging platform for cancer therapy

Ngày tải lên : 28/05/2014, 15:07

... colony-stimulating factor (G-CSF) IL2 fused to diphtheria toxin Anti-CD33 antibody conjugated to calicheamicin Anti-CD20 conjugated to yttrium-90 or indium-111 Anti-CD20 conjugated to iodine-131 Commercial ... target cells relative to normal cells For example, to efficiently deliver liposomes to B-cell receptors using the anti-CD19 monoclonal antibody (mAb), the density of receptors should be in the ... useful antibodies that bind to the same target cells but to different epitopes (a part of a macromolecule that is recognized by antibodies; one receptor may have several epitopes that will be recognized...

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Báo cáo sinh học: "Targeting TNF-α for cancer therapy" potx

Ngày tải lên : 06/08/2014, 19:21

... peptides might also be used to deliver gene therapy vectors into tumors For example, RGD Burton and Libutti: Journal of Biology 2009, 8:85 85.3 Table Angiogenesis factors Factors affecting endothelial ... migration 23-kD glycoprotein derived from fibroblasts and epithelial cells 20 to 25-kD glycoprotein secreted by T cells and natural killer cells; cytotoxic to proliferating ECs Antithrombin (cleaved) ... inhibitors TIMP-1 (TIMP metalloSoluble 8.5-kD collagenase inhibitor peptidase inhibitor 1) TIMP-2 Soluble 21-kD collagenase inhibitor TIMP-3 Extracellular matrix-associated collagenase inhibitor...

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báo cáo khoa học: "Clinical relevance of "withdrawal therapy" as a form of hormonal manipulation for breast cancer" doc

Ngày tải lên : 09/08/2014, 02:21

... exposed to tamoxifen or placebo The tumors regressed initially over months but started to grow towards the end of the study (8 months) on long-term exposure to tamoxifen and placebo Tumors from ... the sensitivity of the tumour cells to therapy and interruptions to therapy allows resurgence of estrogenic stimulation leading to restoration of sensitivity to letrozole on its reintroduction ... apoptosis at a concentration of 10-11 M/L or more of estradiol This is again possible due to activation of crosstalk mechanisms and domination of mitogen-activated protein kinase or growth factors...

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Báo cáo khoa học: "The "Win-Win" initiative: a global, scientifically based approach to resource sparing treatment for systemic breast cancer therapy" pdf

Ngày tải lên : 09/08/2014, 04:21

... suited to LMCs, few suggestions for this are: For older drugs a) To tailor treatment to patients, community and tumor factors (based on clinical, pathological and biological factors) b) To address ... 4-OH-tamoxifen and endoxifen which both have a greater affinity to the estrogen receptors and ability to inhibit cell proliferation when compared to the parent drug The key enzyme in this bio-transformation ... compared to 52 weeks treatment Both arms were similar in outcome [3] We assume that these preliminary results need to be confirmed in a larger sample In addition to the cost savings from the...

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