applications of snake venom proline rich oligopeptides bj pros in disease conditions resulting
The action of snake venom phospholipase a and trypsin on purified myelin in vitro
Ngày tải lên :
24/10/2014, 07:18
... (25pug/mg of myelin protein) *Snake venom (150ug/mg) + trypsin (154ug/mg of myelin protein) tSnake venom+ trypsin (l50.ug/mg)+trypsin (lSgg/mg of myclin protein) Trypsin (15gg/mg of myelin protein) ... Lysophosphatidylcholine (20,ug/mg of myelin protein) Lysophosphatidylcholine (20,ug/mg)+trypsin (lOpg/mg of myelin protein) Snake venom (lOO1ug/mg of myelin protein) Snake venom (lOO,ug/mg)+trypsin (15#ug/mg of ... myelin protein) Phospholipase A (80,cg/mg of myelin protein) Phospholipase A (80jug/mg)+trypsin (I5,pg/ of myelin protein) Snake venom (1504ug/mg of myelin protein) Snake venom (lSO,g/mg)+trypsin...
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Gene regulation of snake venom prothrombin activators
Ngày tải lên :
11/09/2015, 10:03
... AVIT venom toxin whose ancestral protein is the prokinectin protein (Fry 2005) Upon envenomation, the AVIT toxins act as potent agonists of mammalian prokineticin receptors, and their binding ... often result in new ligand-binding specificities (Kini 2002) Chapter I - Introduction RECRUITMENT OF SNAKE VENOM TOXINS FROM BODY PROTEINS Snake venom is a complex mixture of biologically active molecules ... regulation of snake venom prothrombin activators This recruitment of trocarin D from TrFX is one of the many instances which snake venom toxins have evolved from ancestral body proteins by gene...
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Báo cáo khoa học: "General pharmacological profiles of bee venom and its water soluble fractions in rodent models" doc
Ngày tải lên :
07/08/2014, 18:20
... reappearance of the righting reflex Loss of the righting reflex was defined as an inability of a mouse to right itself times within 30 sec, whereas recovery of the righting reflex was defined as the point ... guidelines of the USA National Institutes of Health (NIH publication No 8623, revised 1985) In addition, the ethical guidelines of the International Association for the Study of Pain for investigating ... activity in mice: During the initial period following placement in the activity box, animals of all groups showed increased ambulation that was related to the exploratory phase of being placed in the...
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Proline rich acidic protein 1 in life and death 2
Ngày tải lên :
11/09/2015, 16:05
... progenitors in the fetal small intestinal epithelium (Korinek, Barker et al 1998) In summary, β-catenin/TCF signaling is essential for maintaining the proliferative/undifferentiated state of the intestinal ... variety of bacteria, including some potential pathogens, from the ingestion of food and drink Innate immune mechanisms are thought to be involved in protecting the host from invasion by luminal ... expression of α-defensins, human β-defensins are expressed at multiple sites including intestinal epithelial cells The expression of β-defensins is inducible at sites of infection or inflammation The...
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Proline rich acidic protein 1 in life and death 3
Ngày tải lên :
11/09/2015, 16:05
... and intestinal differentiation 3.1.1 PRAP1 is expressed in epithelial cells of the intestines The expression of PRAP1 in human intestinal tract was studied using immunohistochemistry Paraffin ... the intestinal cells 77 Figure 3.11 PRAP1 is expressed in the epithelial cells of small intestine Representative figure showing PRAP1 immunohistochemical staining in normal human small intestine ... tissue sections of small intestine and colon were used In the small intestine (Figure 3.11), the expression of PRAP1 was found primarily in the epithelial cells at the top of the intestinal mucosa...
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Proline rich acidic protein 1 in life and death 4
Ngày tải lên :
11/09/2015, 16:05
... 3.11 Sequences of the two p53 binding sites located in PRAP1 gene The consensus sequence of p53 was showed in the second row of the table The p53 binding site located in p21 was included as positive ... dose of 5-FU, there was little cell death observed Intriguingly, inhibition of PRAP1 resulted in a significant increase in the cell death, without exerting its effect via the abrogation of S-phase ... that involves both the intrinsic and extrinsic pathways (Figure 3.64) 3.10.2 Inhibition of PRAP1 induces caspase-dependent apoptosis in cells treated with 5-FU We next investigated the involvement...
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Proline rich acidic protein 1 in life and death 5
Ngày tải lên :
11/09/2015, 16:05
... network of tubulin with a distinct microtubule organizing center (MTOC) Similar findings were also observed with the control siRNA treated cells Interestingly, in the cells with overexpression of ... The intensity of staining was also much reduced than that of the control cells The architecture of the microtubulin network was not well-organized as that of the control cells Similar findings ... Identification of PRAP1 binding protein by MS-MS Representative figure showing the identification of PRAP1 binding proteins after performing MS-MS Figure 3.71 PRAP1 physically interacts with Hsp 70...
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Proline rich acidic protein 1 in life and death r6
Ngày tải lên :
11/09/2015, 16:05
... the annexin V-FITC staining assay Annexin V is a phospholipid binding protein that has a high binding affinity for phosphatidylserine (PS) that translocates from inner to outer leaflet of plasma ... epithelial cells of the gastrointestinal tract of both human and rodent, suggesting a conserved function of PRAP1 in the intestine during development Interestingly, the expression of PRAP1 gene ... was detected in the serum of both normal and SLE patients A: Scatter plot showing the distribution of PRAP1 protein level in serum Level of PRAP1 protein in the serum of normal individuals and...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 1
Ngày tải lên :
14/09/2015, 08:41
... scaffolds in vivo • Optimizing the delivery of plasmid DNA encoding bone morphogenetic protein-2 (BMP-2) • Probing the performances of three kinds of delivery methods of plasmid DNA encoding BMP-2 in ... science and bioinformatics (Donald and Mohammad, 2001) Chapter 1.1.1 Cells Tissue engineering utilizes living cells as engineering materials Examples include using living fibroblasts in skin replacement ... developed in this thesis may be of Chapter 18 importance in combining tissue engineering and drug delivery nicely, with potential in clinical applications 1.3 Drug delivery dosage forms In addition...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 2
Ngày tải lên :
14/09/2015, 08:41
... objective of the research project is to conduct an in vitro study of recombinant BMP2 encapsulated in fibrous scaffolds by investigating the effects of HAp content and the different methods of ... days of release F4 seems less integrated and this finding indirectly explains why BMP-2 in F4 was released much faster than all other fibers Chapter 52 Controlled and sustained release of protein ... processes (for instance emulsification and electrospinning as well as post-processing conditions like scaffold handling and incubation) that may result in partial removal of amino acid groups...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 3
Ngày tải lên :
14/09/2015, 08:41
... scaffolds obtained from the electrospinning method, 15mg of each sample of F1-F4 were weighed and dissolved in mL of DMF solution to extract DCM after freeze drying for days inside a Martin Christ ... detected by 0.4 mg/L of 3, 3’-diaminobenzidine in phosphate buffer at a pH of 7.3, in the presence of 0.12 percent of H2O2 Then sections were counterstained with hematoxylin 4.3.3 Statistical ... serum in vivo Sustained release provides the best strategy to maintain high level of BMP-2 in the local area and that is the main motivation to design the release profile of scaffolds This study investigated...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 4
Ngày tải lên :
14/09/2015, 08:41
... the distinct bands present on the gel (Figure 5.6) In other words, the released DNA has survived both the electrospinning process and postprocessing conditions (handling of scaffold, incubations, ... structure of inorganic-organic system as observed in natural bone The mechanical properties of the scaffolds fabricated using different loading methods were also determined As shown in Figure ... together with HAp nanoparticles in order to escape from the direct contact of DCM As a result, in the process of electrospinning, DNA/chitosan nanoparticles are incorporated into fibers together with...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 5
Ngày tải lên :
14/09/2015, 08:41
... indicate that the bioactivity of BMP-2 plasmid released from Chapter 129 all the three kinds of scaffolds was well maintained, which helped improve the formation of new bone and the healing of ... end of the second week was the lowest Interestingly, the concentration increased significantly in the subsequent two weeks, suggesting that the expression of DNA from group C samples was sustainable ... lacunae Original magnification is 100X for all Chapter 123 Besides H&E staining, IHC staining was also performed to check the formation of blood vessels in bone fractures After treatment of bone...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 6
Ngày tải lên :
14/09/2015, 08:41
... cases of scaffolds F1 and F2 To evaluate the effects of the fabrication process (including spray drying, supercritical CO2 foaming, and handling of samples) on the molecular integrity of plasmid ... maintained after going through the processes of spray drying, supercritical CO2 foaming and post-processing conditions (handling of scaffold, incubations, and lyophilization) As shown in Figure 7.7b, ... fabricated by the combination of spray drying and supercritical CO2 foaming techniques and the effects of chitosan incorporation on the following factors was investigated: in vitro release of DNA, cell...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 7
Ngày tải lên :
14/09/2015, 08:41
... encapsulated in foams was in a supercoiled form This kind of DNA conformation may trigger the penetration of DNA molecules into the deeper layer of K20 coating on foam F2 due to the long chain of K20, instead ... equivalents of base, N-methylmorpholine The removal of Fmoc was completed using 20% piperidine in dimethylformamide (DMF) Cycles of deprotection, washings, double couplings, and washings were repeated ... Lysine-based peptides modified foams for tissue engineering and DNA delivery Toward realizing an effective dual system for tissue engineering and DNA delivery, our work presents intriguing findings...
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Applications of electrospinning and supercritical carbon dioxide foaming techniques in controlled release and bone regeneration 8
Ngày tải lên :
14/09/2015, 08:41
... and sustained expression of DNA, which is favorable for DNA delivery and tissue engineering applications In conclusion, this research has shown potential applications of electrospinning and supercritical ... PLGA/chitosan and PLGA/Lysine composites is in form of naked DNA and its transfection efficiency is extremely low, which would hinder their applications in biomedical engineering In future investigations, ... results indicate that the bioactivity of BMP-2 plasmid released from all the three kinds of scaffolds was well maintained, which helped improve the formation of new bone and the healing of segmental...
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Báo cáo khao học: "Influence of the dual arbuscular endomycorrhizal / ectomycorrhizal symbiosis on the growth of Acacia holosericea (A. Cunn. ex G. Don) in glasshouse conditions" pptx
Ngày tải lên :
08/08/2014, 14:20
... fungus Glomus aggregatum growing in a soil collected in Senegal MATERIALS AND METHODS 2.1 Preparation of fungal inoculum A strain of Pisolithus albus COI 024 (Martin, personal communication) was ... stained according to the method of Phillips and Hayman (1970) [23] The extent of colonization was estimated in terms of fraction of root length with visible mycorrhizal structures (length of ... seeds surface disinfected, some plants were contaminated with indigenous rhizobia The main explanation of this contamination was that the irrigation water possibly contained N2-fixing bacteria Root...
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Tài liệu Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons doc
Ngày tải lên :
18/02/2014, 08:20
... neurons In order to determine the localization of PRiMA-linked G4 AChE, sections of tibialis muscle were triple stained for PRiMA, SV48 and AChR The staining of PRiMA was coincident with that of SV48, ... overlapping of PRiMA staining with presynaptic molecules indicates that PRiMA at NMJs is mainly provided by motor neurons Innervation regulates the expression of PRiMA-linked G4 AChE in the spinal ... C-terminal region of PRiMA In addition, no G4 AChE was found in muscles of PRiMA knockout mice, implying that all G4 AChE in muscle is linked with the membrane-anchoring protein PRiMA During muscle...
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Báo cáo khoa học: Identification of crucial residues for the antibacterial activity of the proline-rich peptide, pyrrhocoricin pot
Ngày tải lên :
08/03/2014, 10:20
... activity of a series of pyrrhocoricin analogs, in which either each residue was replaced with alanine (Ala-scan) or arginine-containing modules were inserted into strategically important positions in ... pyrrhocoricin, apidaecin and drosocin [6], and the N-terminal 1–9 fragment of pyrrhocoricin In these derivatives, 5(6)-carboxy-fluorescein was coupled to the a-amino group of an extra N-terminal lysine ... ducreyi in positions 596 and 600, a valine in H in uenzae might allow stronger binding than an alanine in H ducreyi Alternatively, the residues responsible for the positive pyrrhocoricin binding in...
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Tìm thêm:
- hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam
- xác định các mục tiêu của chương trình
- xác định các nguyên tắc biên soạn
- khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn
- khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản
- khảo sát chương trình đào tạo gắn với các giáo trình cụ thể
- xác định thời lượng học về mặt lí thuyết và thực tế
- tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam
- điều tra đối với đối tượng giảng viên và đối tượng quản lí
- điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1
- khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam
- khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu
- nội dung cụ thể cho từng kĩ năng ở từng cấp độ
- xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct
- phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ
- mở máy động cơ lồng sóc
- mở máy động cơ rôto dây quấn
- các đặc tính của động cơ điện không đồng bộ
- hệ số công suất cosp fi p2
- đặc tuyến hiệu suất h fi p2