applications of atomic force microscopy in nanomaterials research
Atomic Force Microscopy in Cell Biology_Methods in Cell Biology Volume 68 pptx
Ngày tải lên :
27/06/2014, 08:20
... understanding of how
nanomachines, like kinesin, convert chemical energy into mechanical movement. So far,
attempts to satisfactorily explain the function of kinesin using the knowledge gained
by ... and
kinematics in addition to the application of other measuring techniques possible. With
this step in the development of scanning probe instruments, the capability of optical
microscopy to investigate ... specific binding, for
instance, to membrane and other molecular structures for a certain time and to track their
motion.
A. Mechanics of Molecular Motors
Kinesins and kinesin-like proteins are of wide...
- 430
- 256
- 0
Atomic Force Microscopy in Cell Biology ppt
Ngày tải lên :
27/06/2014, 17:20
... understanding of how
nanomachines, like kinesin, convert chemical energy into mechanical movement. So far,
attempts to satisfactorily explain the function of kinesin using the knowledge gained
by ... for
instance, to membrane and other molecular structures for a certain time and to track their
motion.
A. Mechanics of Molecular Motors
Kinesins and kinesin-like proteins are of wide interest in ... structure that interacts with its light chain, binding to the cargo.
Several studies on kinesins have shown that the neck and the first hinge region of the
motor play important roles in kinesin directionality,...
- 430
- 285
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 1 ppt
Ngày tải lên :
06/08/2014, 02:20
... Ricci
Atomic Force
Microscopy
Biomedical Methods and
Applications
Imaging Methods in AFM 21
Form the point of view of biomedical applications, interesting experiments
can be performed by coating ... number of data points per line, obtain-
ing at the end a square grid of data points each corresponding to the relative x,
y, and z coordinates in space of the sample surface (11).
Usually during ... Imaging Methods in AFM 13
13
2
Imaging Methods in Atomic Force Microscopy
Davide Ricci and Pier Carlo Braga
1. Introduction
One can easily distinguish between two general modes of operation of...
- 20
- 344
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 2 pptx
Ngày tải lên :
06/08/2014, 02:20
... test pattern (as in Fig. 4) will produce an asymmetric
profile. In the case of contaminants, one often notices an abrupt change of
detail contrast during scanning and a blurring of the image. Sometimes ... atomic force microscopy tips be inspected by atomic
force microscopy? J. Vac. Sci. Technol. B. 9, 1309–1312.
3. Keller, D. and Chou, C. C. (1992) Imaging steep, high structures by scanning
force microscopy ... by inducing stress on one surface of the cantilever as the result
of denser packing of the molecules (8) or a frequency shift in case of dynamic
detection mode (9) as a result of changes in mass.
3....
- 20
- 412
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 3 pdf
Ngày tải lên :
06/08/2014, 02:20
... washed again in PBS before fixing
in 0.01% gluteraldehyde (in S buffer) for 10 min. This is followed by another
wash in PBS before being thoroughly rinsed in deionized and distilled water.
Finally, ... As in the cases described in Subheading 3.1.2., a
soft lever (k
N
< 0.01 N/m) in combination with a low applied force (<1 nN)
will enhance information arising from the softer elements of ... strategy to
gain insight into cytoskeletal dynamics (e.g., refs. 5,12–14). An example of
investigations of slow intracellular dynamics is shown in a sequence of images
in Fig. 3, where the intracellular...
- 20
- 265
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 4 potx
Ngày tải lên :
06/08/2014, 02:20
... imaging difficulties. The inter-
action of glycoaminoglycans chains of the glycocalyx layer with the microscope
tip makes the imaging of the fresh tissue difficult (Fig. 6). When the imaging of
a ... unevenness of the sample sur-
face thus increasing contrast. In addition, information concerning the sample’s
molecular composition can be revealed and help in the interpretation of the effect
of the ... cells
measured by atomic force microscopy: effects of anticytoskeletal drugs and mem-
brane crosslinking. Scanning 20, 389–397.
20. Kuznetsov, Y. G., Malkin, A. J., and McPherson, A. (1997) Atomic force...
- 20
- 319
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 5 doc
Ngày tải lên :
06/08/2014, 02:20
... 95
95
8
Calculation of Cuticle Step Heights from AFM Images
of Outer Surfaces of Human Hair
James R. Smith
1. Introduction
Atomic force microscopy (AFM) is an ideal technique for noninvasive
examination of hair ... providing a wealth of structural informa-
tion not always apparent from electron microscopy. The fine cuticular struc-
ture of human head hair is of interest to those engaged in the fields of
dermatology ... first line consisting of 500 data points of height values
recorded in nanometres. The line of data is then derivatized using a first-order
algorithm to locate the positions of the cuticle steps...
- 20
- 321
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 6 pdf
Ngày tải lên :
06/08/2014, 02:20
... the
recording of pictures with increasing detail. Especially in fluid medium, the
investigation of active cells offers numerous facts. As an example of dynamic
interactions, a series of images ... magnified in Fig. 6).
116 Bischoff et al.
116
Fig. 6. Zooming in on frame B of Fig. 5 (same z scale for both figures). Increasing contrast in the deflection mode indicates
growing roughness of the ... explained in
detail in Fig. 11.
7.9. Cells Can Contaminate or Stick to the Scanning Tip
Occasionally during the scanning procedure, the tip is covered with an in-
definite cluster. Tip-biofouling...
- 20
- 341
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 7 pot
Ngày tải lên :
06/08/2014, 02:20
... 135
nominal setpoint used during imaging in the case of curve 3, we have an inden-
tation of 950 nm, and for curve 4 we find 200 nm. We need to keep these
features and figures in mind in order ... continuously during dem-
ineralization in the wet cell of the AFM. Thus, each pixel of a line and each line of a
scan represent different exposures and offer a continuous record of the demineraliza-
tion ... Bischoff, R., Bischoff, G., and Hein, H J. (2002) Scanning force microscopy
(SFM) visualization of adherently growing cells. Am. Biotech. Lab. 3, 20–22.
20. Bischoff, R., Bischoff, G., and Hoffmann,...
- 20
- 337
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 8 pptx
Ngày tải lên :
06/08/2014, 02:20
... surrounding intertubular dentin, leaving enlarged tubule lumens (Fig.
4B). Exposure increments are continued in steps of 10 s to a minute or more to
follow continued changes in the dentin structure ... ν
i
2
)/E
i
(6)
Indentations can be made at intervals of 1–2 µm using indents of submicro-
scopic size (approx 300–500 nm). The spacing is needed to avoid influence of
one indent on the adjacent indent ... Nanoindentation of Demineralized Dentin
There is considerable interest in measuring dentin or other calcified tissues
as modified by demineralization or hypomineralization. For example, etching
procedures...
- 20
- 332
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 9 docx
Ngày tải lên :
06/08/2014, 02:20
... (1995)
Atomic force microscopy study of fine structures of the entire surface of red blood
cells. Scanning Microscopy 9, 981–988.
5. Siedlecki, C. A. and Marchant, R. E. (1998) Atomic force microscopy ... previous scanning tunneling microscope (5), which provided
information at atomic resolution of specimens that are electrically conducing.
Because SFMs involve interactions between atomic forces (about ... Adapting
atomic force microscopy for cell biology. Ultramicroscopy 82, 289–195.
3. Gunning, P. A., Kirby, A. R., Parker, M. L., Gunning, A. P., and Morris, V. J.
(1996) Comparative imaging of Pseudomonas...
- 20
- 249
- 0
Atomic Force Microscopy in Cell Biology Episode 1 Part 10 potx
Ngày tải lên :
06/08/2014, 02:20
... with
different information (Fig. 3). As outlined in Fig. 3, applying low imaging
forces will result in a more accurate surface visualization (Fig. 3B), whereas
increasing the force will result in obscuring ... daptomycin: atomic force microscopy investigation.
Chemotherapy 14, 336-341.
14. Nagao, E. and Dvorak, J. A. (1999) Developing the atomic force microscope for
studies of living cells. Intern. ... Wisse
1. Introduction
In 1986, Binnig et al. (1) revolutionized microscopy through the invention
of the atomic force microscope (AFM). Subsequently, commercial instruments
of this new imaging technique...
- 20
- 332
- 0
Atomic Force Microscopy in Cell Biology Episode 2 Part 1 ppsx
Ngày tải lên :
06/08/2014, 02:20
... AFM of Protein Complexes 217
217
16
Atomic Force Microscopy of Protein Complexes
Olga I. Kiselyova and Igor V. Yaminsky
1. Introduction
Scanning probe microscopy (SPM) is a rather new family of ... versa. (D) Increasing the inte-
gral gain (see Note 16 during imaging resulted in an artifact-free image and reveal typi-
cal jasplakinolide-induced changes, that is, a loss of jasplakinolide A-sensitive ... marked line in (A).
AFM Imaging of Living Cells 209
3.5.3. Tip-Induced Smearing
In general, the lateral force can wipe away or smear out surface features,
whereas the constant force can deform soft...
- 20
- 297
- 0
Atomic Force Microscopy in Cell Biology Episode 2 Part 2 ppt
Ngày tải lên :
06/08/2014, 02:20
... degree of tilt of the molecules in the gel
compared with the fluid phase because the depression of the tip in a softer fluid
phase varies depending on the composition of this phase. In case of BLES, ... edge of the gel
domains (indicated by letter C in the images) in (A) are not smooth but contain differ-
ent filamentous structure and microdomain (arrow) protruding from such domains as
shown in ... states using a combination of fluorescence and atomic force microscopy
(AFM) (9).
Monolayer films have also become a standard model for studying lipid–
protein interactions and associations in biological...
- 20
- 328
- 0
Atomic Force Microscopy in Cell Biology Episode 2 Part 3 pps
Ngày tải lên :
06/08/2014, 02:20
... (6–9).
Since its invention in 1986, the use of the atomic force microscopy (AFM)
has become a standard technique on various biological applications, including
chromosomes (10–12), not requiring, ... loading force. Do not exceed a loading force of 1 nN.
Start with a loading force of 0.5 nN and then switch to the image. When the
cantilever is in contact with the cell surface, increase the integral ... part of the force curve and the set-point line. This value
(in nm) multiplied by the spring constant gives the loading force (for details, see
Appendix). You should not exceed a loading force of...
- 20
- 315
- 0
