application to high throughput screening and drug discovery
high throughput screening, methods and protocols
... the field of drug discovery, and evolved into the field of high throughput screening (HTS) (1,5–9) Radioligand binding assays have historically been the mainstay of drug discovery and drug development ... provide a means to configure radioligand receptor-binding assays compatible with 96-well and higherdensity arrays to provide higher throughputs, and potentially reduce costs In addition to the SPA ... mainstay of HTS, drug discovery, and drug development References Cook, N D (1996) Scintillation proximity assay: a versatile high- throughput screening technology Drug Discovery Today 1(7), 287–294...
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Báo cáo hóa học: " Research Article Real-Time Perceptual Simulation of Moving Sources: Application to the Leslie Cabinet and 3D Sound Immersion" pot
... of the audio spectrum To simulate air absorption, the control parameters (cutoff frequency and gain) have to be linked to the listener -to- source distance At a given listener -to- source distance x, ... time-dependent and its specific pattern seems to be a highly relevant cue enabling the listener to construct a mental representation of the trajectory [15] Chowning [8] used such a pattern to design ... addition to intensity variations to make a realistic sensation of an an-coming band in his Bolero: the orchestra starts in a low-frequency register to simulate the band playing at a distance, and...
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Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes" pdf
... (NC_011145) Total IS ORF Total IS Different IS A aeolicus VF5 (NC_000918) Total IS ORF procedure While ISsaga is only semi-automatic and requires some user input and expertise, it permits accurate and ... an automatic Glimmer annotation is performed prior to BLASTP and BLASTX Identified ORFs are included in a candidate ORF list The replicon is then subject to BLASTN (filter off, word size and e-value ... computational tools and methods for rapid, highquality IS annotation This is integrated as a module into ISfinder, the prokaryote IS reference centre database [7] and IS repository, which includes...
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báo cáo khoa học: " A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs" docx
... mission The desired screening of thousands of candidate genes would require many person years without automation Relegating this task to a fully automated high- throughput screening system offers ... powdery mildew fungus by automating very time-consuming screening tasks Proved to work dependably and at operational stage now, it provides a novel tool of medium- to high- throughput phenomics in ... can be understood as a proof of concept of how to extend already available microscope hardware to handle challenging screening tasks fully automatically by bringing together research and development...
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báo cáo khoa học: " SNP high-throughput screening in grapevine using the " docx
... assembly and SNP discovery RV conceptualised the project and contributed to the discussion of the results MT supervised the SNP-based marker development and genetic mapping and contributed to the ... genotyping system application in higher plants, which allowed the development of markers anchoring the grapevine genome To date, a few low- and mid -throughput methods based on SSCP and minisequencing ... Fragment Length Polymorphisms) and RAPDs (Random Amplified Polymorphic DNAs), SNPs can be detected through non-gel-based high- throughput assays, saving both time and money [3] Several SNP assay...
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Báo cáo y học: "A novel approach to modelling water transport and drug diffusion through the stratum corneum" pdf
... capillary rise and Pw is the capillary pressure between water and air in the porous medium Thus, we refer to “capillary head”, and the difference between the head pressures at the top and bottom layers ... pressure difference corresponds to 10 × 103 m and 10.002 × 103 m applied to the top and bottom boundaries, respectively These two simulations were done in order to examine Page 12 of 25 Marquez-Lago ... values in order to define the pressure gradient In consideration of the boundary conditions for the top and bottom faces, atmospheric pressure is equivalent to 760 mmHg (or 101,325 N/m2) and the density...
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Báo cáo y học: "Distribution patterns of small-molecule ligands in the protein universe and implications for origin of life and drug discovery" doc
... metabolism-relevant ligands Click here data file bio-ligands Ligands and theand2 of bio-ligands and binding ATP, ligands Additionalfor file 1ownerships of metabolism-relevantADP and block numbers ... diversity of some very common ligands (that is, ATP, NAD and FAD) bound to proteins, Stockwell and Thornton [41] suggested that molecular flexibility is important for ligands to bind diverse proteins ... ACD-SC-derived ordinary molecules; Ligands, sc-PDB-derived ligands; Ligands (≤ 3), ligands covering ≤ folds; Ligands (4-9), ligands covering 4-9 folds; Ligands (≥ 10), ligands covering ≥ 10 folds Implications...
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Báo cáo y học: "Genomics - from Neanderthals to high-throughput sequencing" ppsx
... projects to identify inversion polymorphisms; to map gene-expression variation as quantitative traits; to analyze human demographic history; to detect regions under recent positive selection; and to ... experimentally verifying and enriching genome annotation and understanding how genome sequence relates to whole-organism biology In order to deal with this large volume of functional data and integrate ... standards and sophisticated storage methods are required With the evolutionary history of the genome playing such a major role in its current function, easy access to the reconstructed ancestors...
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1 adaptive mode superposition and acceleration technique with application to frequency response function and its sensitivity
... According to the value of the exciting frequency, the mode truncation can be divided into middle high- mode truncation and low high- mode truncation [3,4] The ‘‘low’’, ‘‘middle’’, and ‘ high ’ frequency ... for many situations where the MSM and MAM are required [5–9] MSM and MAM Assume the eigenvalue and eigenvector matrices of the model defined in Eq (1) are K and U, and K ¼ diagðl1 ; l2 ; ; ln ... respectively, converge to the eigenvectors Up and the eigenvalues in matrix Kpp provided that the initial vectors X ð0Þ are not orthogonal to ¯ q one of the required eigenvectors If the eigenvalue...
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Development of database and computational methods for disease detection and drug discovery
... drug discovery application besides a data storing pool VS is known to contribute to discovery of hits and lead compounds and VS has been investigated and explored intensively Various tools and applications ... Bioinformatics and cheminformatics in drug discovery The combination of random screening and rational drug design have played an important role in drug discovery [44] The traditional drug discovery ... targets and drug of the approved, clinical trial, and experimental drugs These database lead to a better understanding of the disease and benefit for drug discovery Src promotes tumour invasion and...
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On interaction motif inference from biomolecular interactions riding the growth of the high throughput sequential and structural data
... I did and I am indebted to him for that I also wish to thank many friends and colleagues in the Computational Biology Lab for their interesting discussion and warm friendship Huge thanks to Song ... Thanks to its ability to form different structures, RNA can function as catalyst and regulator in nucleic acid processing in addition to its commonly known intermediary role in DNA transcription and ... tedious and expensive methods to apply on the whole protein interaction data of a single organism (called the interactome) Thus it would be beneficial to have a high confidence set of SLiMs to reduce...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... aspects to generate patient and disease specific pluripotent stem cells in two folds One, making such cells helps us in research to understand disease mechanisms, drug screening and toxicity ... hESCs can be used to identify drug targets and test potential therapeutics They can also be used for toxicity testing Also, studying hESCs help us to understand prevention and treatment of birth ... like to express my sincere gratitude to my supervisor associate Prof Cao Tong who guided me through the master’s program He allows me to think independently and is always there to listen and give...
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Development and application of a high throughput assay for discovery of starch hydrolase inhibitors
... DEVELOPMENT AND APPLICATION OF A HIGHTHROUGHPUT SCREENING ASSAY FOR DISCOVERY OF STARCH HYDROLASE INHIBITORS LIU TING TING (BSc, MSc Wageningen University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... 7) To develop the HTS assay, a microplate reader was used to monitor the changes in turbidity in a 96 well microplate It is expected that this method will be applicable to the high- throughput screening ... molecules in units of two or three, and the combined action of salivary and pancreatic amylase leads to the production of large amounts of maltose and maltotriose, and relatively small amounts of...
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Speaking test and some application to vietnamese high school pupil = kiểm tra kỹ năng nói và một số ứng dụng đối với học sinh PTTH ở việt nam
... test and some applications to Vietnamese High School pupils This refers to the kinds of people that candidate is expected to be able to speak to (For example, native speakers of the same age and ... Speaking test and some applications to Vietnamese High School pupils interview to a close, because it is no use to subject them to a longer ordeal On the other hand, the test intents to see what ... important and necessary to help my students develop their speaking skills and testing their speaking ability Therefore, I decide to choose the topic speaking test and some applications to Vietnamese High...
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Báo cáo y học: "High-throughput RNA interference screening using pooled shRNA libraries and next generation sequencing" ppsx
... screening approaches, often called barcode screening Barcode screening offers improvements in speed and scale compared to plate-based screening In barcode screening, a large population of cells is ... divided into reference (vehicle treated) and test (drug treated) sets Alternatively, in a simple viability screen, a sample of cells can be taken and stored for analysis at each passage, to generate ... were taken to assess the cell number (to construct growth curves) and the percentage of GFP positive cells (to assess the number of cells required to maintain hairpin representation) To minimise...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
... [13] and in human macrophages [14] Unlike CCR5, the CXCR4 receptor has only one known ligand, the α-chemokine 'stromal cell derived factor' (SDF)-1 Since the natural CCR5 and CXCR4 ligands and ... astroglioma cell line expressing both CCR5 and CXCR4 in addition to the cellular CD4 receptor, and we demonstrated its usefulness as a tool to evaluate CCR5 and CXCR4 antagonists Results Establishment ... valuable as a tool for high- throughput evaluation of new CCR5 and CXCR4 inhibitors and in vitro evaluation of their therapeutic potential in combination anti-HIV therapy Materials and methods Viruses...
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Báo cáo y học: " Kinetic modeling of tricarboxylic acid cycle and glyoxylate bypass in Mycobacterium tuberculosis, and its application to assessment of drug targets" ppt
... (Rv3339c) and ICD2 (Rv0066c), and two isoforms of ICL [17,18], ICL1 (Rv0467) and ICL2 (Rv1915 and Rv1916), in M tuberculosis H37Rv strain The requisite co-enzymes and co-factors were assumed to be ... starts to increase after VfICD1 and VfICD2 have fallen to approximately 30% of the original values, and becomes equal to Page of 11 (page number not for citation purposes) Theoretical Biology and ... (1) The ratio of inhibitor concentration to inhibitor constant (I/ KI) was assumed to be the same for both ICL1 and ICL2 Two simulations were performed, one with VfICD1 and VfICD2 kept at 2.5%,...
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High Order Sliding Mode Observers and Differentiators–Application to Fault Diagnosis Problem
... make high order sliding mode differentiators appealing in fault diagnosis High Order Sliding Mode Observers and Differentiators 323 Although high order sliding mode observers and differentiators ... Rp is the output vector, and u ∈ Rm is the input vector (the output of actuators), and d ∈ Rq is a bounded unknown input vector which may consist of system uncertainties and/ or disturbances We ... With an intention to use high order sliding mode observers and differentiators to solve difficult fault diagnosis problems, the design of a second order sliding mode observer and a high order sliding...
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Methods to improve virtual screening of potential drug leads for specific pharmacodynamic and toxicological properties
... alternative to high- throughput screening (HTS) and combinatorial chemistry because virtual screening can identify drug candidates in a fast and cheap manner A limitation of computational virtual screening ... known In addition, a broad range of QSTRs and regulatory tools have been developed which include: acute and aquatic toxicity, receptor-based toxicities, and human health effects [20] There is still ... trees should be grown and the number of descriptors to be taken from the square root of the total descriptors [106] RF can handle large number of training data and descriptors Besides classifying...
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High depth resolution rutherford backscattering spectrometry with a magnet spectrometer implementation and application to thin film analysis 2
... accelerator tank is filled with a heavy non-toxic insulating gas SF6 at a pressure of bar SF6 has a dielectric strength approximately 2.5 to times that of air, and serves to prevent corona buildup and ... and analyzer tables, a beam profile monitor (BPM) and a faraday cup measure the beam profile along the x-y plane and the total beam current respectively This facilitates the proper steering and ... terminal relative to the laboratory ground, while a capacitative pickup unit (CPU) measures the ripple of the terminal voltage and feeds the signal back to the voltage regulator controlling the...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu