annotation metabolic pathways and evaluation of protein domains
báo cáo khoa học: " The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana" pdf
Ngày tải lên :
11/08/2014, 11:21
... obtain an Page of 10 integrative understanding of the responses of leaf growth to global warming, we examined the effects of simulated climatic warming on SOD and CAT activities, contents of MDA, proline, ... palisade and spongy tissues) size and number of chloroplasts per cell were determined from 300 cells Chloroplast length and width, area of chloroplast profile, and ratio of total starch grains per chloroplast ... elevated temperatures I and II (Table 1, Figure 3A-D) Starch grains accounted for an average of 15% and 13% Page of 10 of the chloroplast profile at elevated temperatures I and II, respectively;...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
Ngày tải lên :
25/10/2012, 11:10
... also proved that majority of fusion protein was present in inclusion body (Fig 2B) Figure Maps of N-terminal His-tagged gAd-GLP-1-A fusion protein and gAd-GLP-1-A fusion protein (A) N-terminal His-tagged ... the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance of GLP-1 Studies ... part of fusion protein or both of them In other words, we need to know whether each part of the fusion protein play glucose-lowering effect separately We also need to know whether the half-life of...
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Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
Ngày tải lên :
19/02/2014, 16:20
... (EMSA) and RdRp assays, and examined the influence of different cis-acting elements on binding of NS5B protein and RNA synthesis in vitro Materials and methods Expression and purification of NS5B proteins ... mechanism of initiation from the very 3¢ terminal of 3¢ UTR might be one of peculiarities of CSFV replication because the sequence of the 3¢ terminal of 3¢ UTR is necessary for binding of NS5B protein ... these minus-strand RNA fragments (Fig 4C) Therefore, the 3¢ ÔCATATGCTCÕ sequence of 3¢ terminal of the minus-strand genome is essential to binding of the CSFV NS5B protein The minus-strand 3¢ UTR...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
Ngày tải lên :
21/02/2014, 15:20
... structure (strands B, C and part of strand D), along with part of the distant I strand in the C-terminus region that includes Cys160 and connects to the leftovers of strand D via a disulfide bridge ... structure of beta-lactoglobulin and its similarity to plasma retinol-binding protein Nature 324, 383–385 Pervaiz, S & Brew, K (1985) Homology of beta-lactoglobulin, serum retinol-binding protein and protein ... proteolytic/thermal treatment of BLG at relatively high enzyme/ BLG ratios (1 : 10 and : 20) and at the highest temperature compatible with retention of enzyme activity and with the reversibility of structural...
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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx
Ngày tải lên :
07/03/2014, 15:20
... kinetics of reduction of cytochromes b and c1 were measured in the mutant and revertant strains The rates of reduction of cytochromes c + c1, in mutants and revertants, was decreased to 35% of the ... interfacial protein protein interactions between the hinge region of ISP and the extramembranous cd2 helix of cytochrome b are important for maintaining the structure of the hinge region and, by ... spectra of the wild type and mutant cells Optical spectra of cell suspensions of the wild type (wt) and mutant (G167E) were obtained as described in the Experimental procedures wt red, Spectrum of...
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Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf
Ngày tải lên :
15/03/2014, 23:20
... various proteins in addition to TcDHH1, including heat shock proteins, mRNAbinding proteins, initiation and elongation translation factors, ribosomal proteins and metabolic proteins Abundant proteins, ... that proteins directly or indirectly associated with TcDHH1 include those of diverse function, e.g translation-related factors, cytoskeleton proteins, heat shock proteins and metabolic proteins ... trypomastigotes (Meta) and amastigotes (Ama), probed with antiserum against TcDHH1 protein (1 : 100 dilution) The extracts were standardized and all lanes were loaded with 10 lg of protein (B) SDS ⁄...
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Cd toxicity effects on growth, mineral and chlorophyll contents and activities of stress related enzymes
Ngày tải lên :
15/03/2014, 23:22
... level of protein about mg g−1 FW was then maintained Protein contents Enzyme activities and isoenzyme analysis The total soluble protein content was examined in leaf and root samples Roots and ... and Khan N S 1983 Influence of lead and cadmium on the growth and nutrient concentration of tomato (Lycopersicon esculentum) and egg-plant (Solanum melongena) Plant Soil 74, 387–394 Khan D H and ... of Zn and Cd in bean (Van Assche et al., 1988) and to the level of Cu in maize (Mocquot et al., 1996) Enzymes involved in the intermediary metabolism are also altered by toxic amounts of Zn and...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
Ngày tải lên :
16/03/2014, 05:20
... blot analysis of the hemolymph and ovary total protein of dsRNA-injected females (A) Hemolymph sample of NaCl ⁄ Piinjected and dsRNA-injected females Lanes 1–3: NaCl ⁄ Pi-injected and 4–6 dsRNA-injected ... monodon As there is a high degree of sequence similarity and structural conservation of these genes and gene products, confirmation of the true identity and function of these neuropeptides remains ... injection (Figs 4A and 5A) In conclusion, the use of recombinant protein or RNAi alone may not be sufficient to confirm the function of a neuropeptide The combined use of recombinant protein and RNAi described...
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Báo cáo khoa học: A (1fi3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of fibrinogen-like protein and epidermal growth factor gene expression pot
Ngày tải lên :
16/03/2014, 16:20
... (1fi3)-b-Dglucan-binding protein; subsequently, a gene encoding a fibrinogen-like protein, and also one for a cytokine, are strongly expressed Materials and methods Chemicals and enzymes The sources of chemicals and ... prepared and subjected to PAGE in the presence of a low concentration of SDS and in the absence of b-mercaptoethanol After size separation (Fig 2; lane a), the proteins were transferred and – after ... Coomassie Brilliant Blue (Fig 1A) and no change in the banding pattern and their intensities occurred Fig Phosphorylation of a 32 kDa protein after incubation of sponge tissue with curdlan Tissue...
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Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding pot
Ngày tải lên :
16/03/2014, 18:20
... features of proteins of the GH72 family and demonstrate that the most N-terminal highly conserved cysteine is crucial for the folding and maturation of Gas1p Materials and methods Strains and growth ... determined The effects of the replacement of C74, C105 and C265 with a serine residue on the expression and complementation of the mutant phenotype of the gas1 null mutant of S cerevisiae were ... consisting of a structure that contains both a-helices and b-sheets, and that the latter ones predominate Quantification of disulphides and free sulphydryl groups present in Gas1p Proteins of Family...
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Role of C-Reactive Protein and Procalcitonin in Differentiation of Tuberculosis from Bacterial Community Acquired Pneumonia potx
Ngày tải lên :
22/03/2014, 18:20
... sensitivity of 90.0% and a specificity of 58.9%; at a cutoff value of 0.25 ng/mL, the PCT concentration had a sensitivity of 93.1% and a specificity of 59.6% (Fig 1, Table 2) CRP and PCT concentrations ... respiratory secretions and 13 patients Kang YA, et al C-reactive protein and procalcitonin for the diagnosis of tuberculosis 339 Table Clinical and laboratory characteristics of the participants ... Clinical and laboratory characteristics of the patients Of the 87 patients who met the inclusion criteria, 57 had bacterial CAP and 30 had pulmonary TB The median age of the bacterial CAP and pulmonary...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
Ngày tải lên :
30/03/2014, 11:20
... form of dimers [5,14] To understand the importance of capsid protein protein contacts for the stability of coat protein, we determined the stability of dlFG and compared it with the stability of ... presence of both DNA and bis-ANS decreased the stability of the protein (Fig 7B) Effects of high temperature on the secondary and tertiary structure of dlFG and sc-dlFG in the absence and presence of ... protein RNA and protein protein interactions in virus stability, measuring the effects of urea, GdnHCl and high pressure on the structure and stability of whole particles (bacteriophage MS2 and VLPs) and...
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basic protein and peptide protocols
Ngày tải lên :
10/04/2014, 11:03
... otherwise slow up the rate of sample electrophoresis and also spoil the resolution of protein bands As mentioned earlier, protein bands in this system are often slightly misshapen, and this problem is ... wells at the very ends of the row of wells, for samples in these often suffer distortion during the electrophoresis 14 Be wary of the dangers of electric shock and of fire, and of the neurotoxic acrylamide ... aggregation and precipitation of proteins Also, the pH of the stacking gel (pH 6.8) may affect the activity of the protein of interest If this is thought to be a problem (e.g., the protein cannot...
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protein and peptide analysis by mass spectrometry
Ngày tải lên :
11/04/2014, 10:11
... be capable of the analysis of large underivatized peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake toxms ... Separation and solvatton of analyte molecules m the solid phase, avoiding agglomeratron of analyte material, Uptake of laser energy used for desorptton and ronizatlon of matrix and analyte molecules, and ... procedures generally used m protein chemistry m terms of senstttvtty and acceptance of solvents, detergents, and buffers, They must be able to handle impure samples and complex mixtures Last,...
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Báo cáo sinh học: " Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition potx
Ngày tải lên :
18/06/2014, 18:20
... attributed to the expression of the A12L protein and its essentiality in virus replication Essentiality of A12L protein and AG/A cleavage in VV replication The sequence alignment of the A12L open reading ... recruits and organizes the first recognized membrane and induces the correct formation of viral genome content through the proteolysis of viral core/membrane proteins However, the essentiality and ... the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV membranes G7L, a phosphoprotein in...
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Báo cáo sinh học: "Alpha-1 antitrypsin protein and gene therapies decrease autoimmunity and delay arthritis development in mouse model" potx
Ngày tải lên :
18/06/2014, 19:20
... (DK58327) and University of Florida Office of Research Author details Department of Pharmaceutics, University of Florida, Gainesville, FL 32610, USA 2Department of Pathology, University of Florida, ... immunization) of the experiment, these results showed that treatment of hAAT protein (Prolastin®) led to a delayed arthritis onset and amelioration of disease progression in CIA mouse model Human AAT Protein ... the effect of hAAT on autoantibody production, we evaluated the levels of anti-CII autoantibodies in total Ig, and IgG1 and IgG2a subclass at early (day 35) and late (day 49) stages of the disease...
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Báo cáo hóa học: " Vaccinia virus A12L protein and its AG/A proteolysis play an important role in viral morphogenic transition" pdf
Ngày tải lên :
20/06/2014, 01:20
... attributed to the expression of the A12L protein and its essentiality in virus replication Essentiality of A12L protein and AG/A cleavage in VV replication The sequence alignment of the A12L open reading ... recruits and organizes the first recognized membrane and induces the correct formation of viral genome content through the proteolysis of viral core/membrane proteins However, the essentiality and ... the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV membranes G7L, a phosphoprotein in...
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Báo cáo hóa học: " Viable chimaeric viruses confirm the biological importance of sequence specific maize streak virus movement protein and coat protein interactions" pdf
Ngày tải lên :
20/06/2014, 01:20
... determinants of pathogenicity in maize resided in the MSV movement (MP) and coat protein (CP) genes Among the bipartite begomoviruses pseudorecombination of A and B components has formed the basis of many ... were assessed in terms of infectivity and symptomatology, and evidence of biologically-important specific interactions between the MSV MP and CP is presented Results Viability of chimaeric genomes ... comprising both MSV-Kom mp and cp exchanged into the MSV-Set genome showed the highest AUDPC of all the chimaeric constructs, achieving 80% and 85% of the AUDPC figures of KNco and SNco respectively...
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Statin Therapy, LDL Cholesterol, C-Reactive Protein, and Coronary Artery Disease pot
Ngày tải lên :
27/06/2014, 00:20
... changes in the levels of atherogenic lipoproteins, CRP, and HDL cholesterol and the rate of progres- Table Baseline and Follow-up Values for Intravascular Ultrasonographic End Points and Change in Values ... important implications for understanding the pathogenesis of the progression of atherosclerosis and the mechanism of benefit of statin therapy The Pravastatin or Atorvastatin and Infection Therapy (PROVE ... inflammation/ CRP evaluation (PRINCE): a randomized trial and cohort study JAMA 2001;286:64-70 Ridker PM, Rifai N, Rose L, Buring JE, Cook NR Comparison of C-reactive protein and low-density lipoprotein...
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