... expressedGAPDH–citrine (chimeric protein with N-terminalGAPDH and C-terminal citrine) and citrine–GAPDH(chimeric protein with N-terminal citrine and C-terminalGAPDH) were localized in the cytoplasm. PGK–ceru-lean ... of linkers. In the linker con-taining seven amino acids, a Pro-Pro sequence that isnot contained in other linkers is incorporated. This lessflexible structure may inhibit fluorophores to allow ... GAPDH–citrine and PGK–cerulean. Localization of endogenous GAPDH and PGK in HeLa cells was examined by using antibodies against GAPDH and PGK, respectively, followed by incubation in TRITC-conjugated...
... equipment costs involved with splicing, such as purchasing splice machines (up to $30k per unit), cleaving machines, and stripping equipment. Adding up time of installation, labor, and capital ... services.This paper explains the benefits of implementing plug -and- play techniques, beginning at the CO and working through the OSP and into the subscriber premise. Using plug -and- play architectures ... standards and manufacturing improvements that result in lower insertion and return loss.Hardened adapters are integrated into environmentally sealed, preterminated multiport service terminals....
... well be Six Sigma. ” Six Sigmaand design for Six Sigma: what is it? In the recent past, there has been tremendous focus on Six Sigma initiatives by many different companies in various industries. ... . In Chapter 3, we focus on the SixSigma roadmapfor product and process development. Quality Engineering tools and their linkages to the SixSigma roadmap are introduced. After explain-ing ... become world-class in their industry, if not all of industry.For example, Toyota uses lean manufacturing principles to reduce its inventory levels, and Dell is well known for its custom computerassembly...
... throughoutanorganization in identifying and eliminatingproductionwastes.Whenenvironmental wastes are included, Lean andSixSigma become powerful vehicles for engaging employees in identifying and implementing ... bottom line performance. • Businesses are increasingly experimenting with “paths to sustainability” incorporating corporatesocialresponsibility and “triplebottomline”thinkingintothecorefabricofbusiness ... • Shipbuilding&RepairAlthough it originated in manufacturing, Lean production has been rapidly expanding to service industries,includinghealthcare,banking,insurance, and evengovernmentagencies.Overthepastfive...
... of Six Sigma, ” and the other called “Design for Six Sigma, ” which areboth best-sellers in Korea. In 2001, I had the honor of beinginvited to the “Symposium on Concept and Management of Six Sigma ... Robert Galvin devel-oped a concept called Six Sigma. After some internal pilotimplementations, Galvin, in 1987, formulated the goal of“achieving Six- Sigma capability by 1992” in a memo to all Motorola ... rights to order, select and buy goods and services. Especially, in e-business, the cus-tomer has all- mighty power. Competition in quality and pro-ductivity has been ever -increasing. Second-rate quality...
... skills, including understanding individual behaviour, values and attitudes; interpersonal communication skills (understanding thedynamics of transactions between individuals and using these in coaching ... uncompromising, Zander has ‘disappointed’ some analysts by refusing to say what he thinksMotorola should do, but instead insisting on listening to the peopleinside the organization. The parallels ... performance was maintained.We applied the approach in the nuclear industry, in motor manufactur-ing, in an assessment of the potential for transforming the inland mail, and in three different...
... on six sigmaand quality methods, including the in- company presentations,who kept alive my interest and faith insix sigma. I wish them success in implementing sixsigma tools and methods in ... 89calculations and their relationship to six sigma 3.3.7 Use of control charts in factories that are 91approaching six sigma 3.4 Using TQM Techniques to Maintain SixSigma 91Quality in Manufacturing3.4.1 ... was involved with many compa-nies and organizations whose engineers and managers were usingoriginal and ingenious applications of sixsigmain traditional design and manufacturing. Out of these...