and belasco j g 2005 microrna regulation of the mammalian lin 28 gene during neuronal differentiation of embryonal carcinoma cells mol cell biol 25 21 9198 9208
Transplantation of skeletal myoblast in ischemic heart disease 1
... and doubling time 3.2.2 Immucellular dynamics of allogeneic and autologous myoblast transplantation 3.2.3 Elisa for rat IgG and IgM after allogeneic and autologous SkM transplantation 3.2.4 Cell ... immunostaining and doubling time of rSkMs Figure 3.27 Desmin immunostaining and flow cytometry assay for the purity of rSkMs Figure 3 .28 Time observation of the infiltration of macrophages, CD8+, and ... purity Figure 3.5 MHC I staining for hSkMs and myotubes Figure 3.6 MHC II staining for hSkMs and myotubes Figure 3.7 The labeling of SkMs by DAPI, BrdU, and lac-z gene Figure 3.8 Creating and confirming...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... using the following primers: LTA-1F, 5¢-CTC GAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA1R, 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former, and LTA–M2F, 5¢-CAGCTGTTTTGCTTGAATTATG-3¢ and LTA–2R, 5¢-GAATTCATTATGTTTCAGGTTCA ... examine the endothelial cell biology of the mouse, and will accelerate the molecular and cellular analysis of ECs and their heterogeneity in various vascular beds 1994 Construction of the transgene ... enzymatic digestion and sequencing Generation of a transgenic mouse line carrying the CAG–b–geo–tsA58T Ag transgene SalI-digested pCGX harboring the tsA58T Ag cDNA was resolved by electrophoresis, and...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot
... 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢ b Primer pair: 5¢-GCCATTT TAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢ c Primer pair: 5¢-CATCCATAGCAGATAACAGTC-3¢ and 5¢-T CCCAAAGCTCATGTCATAAG-3¢ ... O-glycosylations using the algorithm of Nielsen et al [28] , the extracellular region of bovine and human MUC15 offer 22 and 14 O-glycosylation sites, respectively The majority of these potential O-glycosylation ... results suggest a mechanism whereby alternative splicing regulates the relative levels of both the receptor and its secreted cognate binding protein, generated from the one and same gene, and thereby...
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cancer cell culture, methods and protocols
... 5'-GAAACTGGCCTCCAAACACTGCCCGCCG-3' 5'-GTCTTGTTGGAGATGCACGTGCCCCTTGC-3' D17S5-F D17S5-R 5'-AAACTGCAGAGAGAAAGGTCGAAGAGTGAAGTG3' 5'-AAAGGATCCCCCACATCCGCTCCCCAAGTT-3' D2S44-F D2S44-R 5'-AGCAGTGAGGGAGGGGTGAGTTCAAGAG-3' ... 5'-AGCAGTGAGGGAGGGGTGAGTTCAAGAG-3' 5'-GAAAACACTTCAGTGTATCTCCTACTCC-3' COL2A1-F COL2A1-R 5'-CCAGGTTAAGGTTGACAGCT-3' 5'-GTCATGAACTAGCTCTGGTG-3' PAH-F PAH-R 5'-GTTATGTGATGGATATGCTAATTAC-3' 5'-GTGGTGTATATATATGTGTGCAATAC-3' ... confirm the proposed origin of the cell line but to check for the presence of contamination with other cell lines Other levels of characterization include the monitoring of the general features of the...
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cell and tissue culture
... Cell line NIH3T3 Cell line HeLa Cell line J5 58L Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell line WI-38 Cell line Namalwa Cell line BHK -21 Cell line MDCK Cell line GH3 Cell line ... fusepreferentially with the dividing cells of the spleen, which, of the l3 cells, are principally the Ig-secreting, pla~ue-formingcells Cells are cultured in R I 1640 with glutamine and 10% Fl3S The cells are ... asmonoclonal antiof bodies for diagnostic use In addition, the use of animal cells is expanding in a wide range of other applications: drug screening,tissue en~ineering, gene therapy, ology and traditional...
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introduction to cell and tissue culture
... in mammalian cell culture of the range of cell "behaviors," their inherent stability, the ability to genetically manipulate cell properties, and the technical challenges of growing mammalian cells ... Page Figure 1.1 A photograph of the tissue culture apparatus such as that used at the turn of the century formed with cells, including production of genetically altered cell lines through mutagenesis ... Dr Gordon Sato, who introduced us to the joy of cell culture and the infinite variety of interesting things to with cells A note on the figures: The graphs and tables presented throughout the...
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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf
... part in the development of the intestinal damage (villous atrophy) regulating the deposition, degradation and remodeling of the ECM through the secretion of collagen, MMPs, TIMPs, and TG2, usually ... intestinal flora, the manual management of the endoscope and endoscopic forceps, and their passage through the endoscopic channel together with the patients' gastric juice and saliva For these reasons ... http://www.translational-medicine.com/content/7/1/40 Q-Banding Cells in log phase were cultured with 50 μL of colchicine for hours and mitotic cells were gently blown with a pipette Cells were centrifuged at 235 × g for 10 minutes and the supernatant...
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báo cáo hóa học: "Fibrillar beta-amyloid peptide Aβ1–40 activates microglial proliferation via stimulating TNF-α release and H2O2 derived from NADPH oxidase: a cell culture study" doc
... phosphorylation and prolongation of mitogenic signalling [31,33] Thus CD45 might be one potential target for hydrogen peroxide in regulating microglial proliferation Proliferation of microglia is a ... n fA Figure Production of hydrogen peroxide by isolated microglial cells in the presence of Aβ1–40 peptide Production of hydrogen peroxide by isolated microglial cells in the presence of Aβ1–40 ... preparing the manuscript AT synthesized the Aβ1–40 peptide MS synthesized EUK-8 and EUK-134 and helped to write the manuscript providing useful suggestions GCB conceived of the study, and participated...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
... these findings and to understand the complexity of the emerging diversity of HIV in this region of high diversity Finally, although higher frequencies of drug resistance in patients harboring ... belonged to CRF02_AG, 6% subtype F2 and 41% were Unique Recombinant Forms (URFs) (Table 1) It is interesting to note that majority of viruses were recombinants of CRF02_AG with gene segments of other ... (07CMLPH 128) was an R5 virus but genotype prediction scored it as X4 tropic because of mutations in the V3 region and a net charge of For these field isolates the combined 11 /25 and charge rule...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... subepithelial cells, suggesting that genital epithelial cells are not susceptible to HIV infection and play no part in the transfer of infectious virus across the epithelium As a result, these cells ... H9+ cells (from Dr Robert Gallo), HeLa-CD4-LTR-β-gal cells and the viral strains HIV-1IIIB and HIV-1AZT-RV HIV-1IIIB was propagated by sub-culturing in the MT-2 and H9+ cells, according to the ... virucidal agent that attaches at the viral mem- Page of 11 brane, [36,69] and may protect the natural barrier of the genital epithelium, therefore inactivating the ability of the HIV virus to reach the...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... formation of the skeleton during embryonic or fetal organogenesis Aggregation of cells to a critical size is a fundamental step in initiating organogenesis of vertebrates [33] Hall and Miyake ... microspheres during cultivation Aggregation of cells is the pivotal stage in the development of skeletal tissues and the primary resource from which the skeleton is built and through which the skeleton ... in tissue engineering Int J Oral Maxillofac Implants 2006, 21: 225- 231 26 Boudreau NJ, Jones PL: Extracellular matrix and integrin signalling: the shape of things to come Biochem J 1999, 339(Pt...
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Báo cáo khoa học: " Thermal stability and inactivation of hepatitis C virus grown in cell culture" ppsx
... of these cell lysates in three consecutive passages of inoculated cells (Table 3), while the control cell lysate prepared by freezing and thawing in the absence of any detergents retained high ... RT, cells were washed extensively with PBS and then incubated with an FITC-conjugated goat anti-rabbit IgG (Beijing Zhongshanjinqiao, China) at 1:100 dilution for h Following PBS washes, the ... previous report dealing with the J6 / JFH1 chimeric virus [10] The ability of HCVcc to survive various environmental temperatures warrants precautions in handling and disposing objects and materials...
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Báo cáo y học: "Calcium deposition in osteoarthritic meniscus and meniscal cell culture" pps
... by targeting the calcium deposits (physical target) or by targeting the cells (biological target) Targeting the calcium deposits directly will inhibit the growth of the calcium deposits and reduce ... reduce the detrimental downstream biological effects of these calcium deposits Targeting the cells at the cellular, genetic or epigenetic levels will not only inhibit the formation and growth of ... bone suggests altered bone remodelling, WNT and transforming growth factor-beta/bone morphogenic protein signalling Arthritis Res Ther 2007, 9:R100 Noble J, Hamblen DL: The pathology of the degenerate...
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Báo cáo y học: "Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts" pdf
... patients and all experiments Examples of the regulated genes and gene groups are listed in Table The list of regulated genes is available at the Arthritis Research and Therapy homepage Here, the genes ... to 12.0% of the expressed genes (Figure 4) Proliferation of RASF during cell culture passaging The cell doubling rate was measured during passages to as described above by counting the cells each ... nitrogen affected mainly the gene expression of the first passage of the cells, possibly due to the stress of thawing and the remaining DMSO until the DMSO was completely removed Thereafter, the cells...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
... random occasions, usually family gatherings When measuring time occurs the grandchildren stand against the board, a line is drawn at the top of their head, and the date of the line is noted The ... ciated with the legend of Figure and Figure should be associated with the legend of Figure 2.] Growth of mammalian cells during the division cycle Conlon and Raff [20] have proposed a linear growth ... support the proposal of linear growth or bi-linear growth during the division cycle Of course it is very likely that even at the doubling of a gene there are other limiting factors and there is...
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Cell isolation and expansion using dynabeads
... Circulating endothelial cells General Colorectal cancer cells Mammary carcinoma cells General CD4+ T cells CD8+ T cells Myocardial endothelial cells Embryonic endothelial progenitor cells Skin endothelial ... Mouse regulatory T cells (CD4+CD25+) Mouse B cells Mouse NK cells Mouse dendritic cells Mouse endothelial cells Cell subset General Mammary adipose endothelial cells Hepatic endothelial cells Circulating ... remain on the cells throughout the duration of downstream experiments The binding of antibodies or ligands to cell surface antigens can lead to clustering of receptors, triggering of signaling pathways...
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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF SCIENCE ... potency of stem cells 1.1.2 Fundermental features of stem cells 1.1.3 Signalling pathways modulating pluripotency in stem cells 1.1.4 Transcription factors controlling pluripotency of stem cells ... hybridization of Oloct4 during embryogenesis 75 Fig 3-7 In situ hybridization of Oloct4 during gametogenesis 77 Fig 3-8 Titration of rabbit antiserum against medaka Oct4 using ELISA 79 Fig 3-9 Protein...
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Phytohormones on cell growth and taxol accumulation via cell culture of taxus wallichiana zucc
... if the interested product is a pigment (Fujita et al., 1984) Cloned cells using cell aggregates of Coptis japonica (Yamada and Sato, 1981) which grew 25 faster and produced a higher amount of ... looking closely at my thesis writing and offering outstanding suggestions for improvement I would like to acknowledge the International University through Science and Technology Development Program ... phenolics, hampering the growth of calli and blackening or browning the medium and explants after a certain period that results in death of the cells (Gibson et al., 1993) Use of 28 anti-oxidants...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... containing polysaccharide One milligram of the partially purified polymer contained 309.7 g of protein, 160.2 g of neutral sugar, 58.5 g of hexosamine, 30.8 g of uronic acid and 3.8 g of phosphorus ... Hosenbrough, N J. , Farr, A L and Randall, R J (1951) Protein measurement with the Folin phenol reagent J Biol Chem., Vol.193, No.1, 265-275 Nam, J S., Kwon, G S., Lee, S O., Hwang, J S., Lee, J D., ... profile of the pronase-digested polymer obtained using a Bio-Gel P-100 column As compared with the intact polymer (Fig 1A), the peak of the absorbance at 280 nm showing protein fell against that of...
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Personality Traits and Workplace Culture
... Extraversion • • • Friendliness: The ability to quickly establish relationships with other people Gregariousness: The level of social engagement High scorers enjoy the company of others and tend to be ... responsibilities, the unit of analysis might be the corporation, the division, the department, or the functional arena The first column of the table contains a listing of the organizational culture ... extent to which the members of the organization are comfortable with uncertainty and risk taking • • • Are there lots of rules and regulations that explicitly define the way "things should be done...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose