and alkali induced dna cleavage and determination of cross linked nucleotide

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

Ngày tải lên : 07/03/2014, 03:20

... sheets, subjected to TLC and quantication of radiolabelled ADP and AMP The kinetics of [3H]AMP and [3H]ADP formation in the absence of tRNAGlu (black symbols) or of both L-Glu and tRNAGlu (grey symbols) ... sequence and the mass of the fragments, and from the kinetics of the process (Figs S7S9 and Table S5) we concluded that the main sites of proteolytic cleavage are at the C-terminus of the predicted ... GluRS and GluTR, whose spectrum and DLS signal are shown in Fig S11 The migration positions of GluTR and GluRS, were determined by comparison with those of a homogeneous sample of GluRS (S) and of...

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analysis and determination of the stress intensity factor of load-carrying cruciform fillet welded joints

Ngày tải lên : 24/05/2014, 20:40

... values of stress intensity factor (KI) increased with the increase in crack size The path of crack propagation in case of root crack is longer than that in cases of toe and cold-lab cracks Values of ... cases of toe and cold-lab cracks 35 Journal of American Science 2014;10(1) http://www.jofamericanscience.org Motarjemi, K., Kokabi, A H., Ziaie, A A., Manteghi, S and Burdekin, F M.: Comparison of ... element method, and is one of the most popular techniques used to calculate the stress intensity factors in numerical studies of fractures [10] The site and curved crack growth paths of continuous...

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Separation and determination of the physico chemical

Ngày tải lên : 01/07/2014, 13:23

... (2005) 1039–1044 of the lack of separation of mixtures of pigments However, the fractions that contained a mixture of the pigments were concentrated and rechromatographed 3.3 Purity of curcuminoid ... support and CNPq for the undergraduate and research fellowships References Ahsan, H., Parveen, N., Khan, N U., & Hadi, S M (1999) Prooxidant, antioxidant and cleavage activities on DNA of curcumin and ... absorptivities A1% of 1488, 1468 and 1445 gLÀ1 for the C, DMC and BDMC, respectively 3.4.2 Infrared spectra The infrared spectra are in conformity with the structures of C, DMC and BDMC The absence of absorptions...

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Báo cáo y học: "Electroconvulsive therapy and determination of cerebral dominance" pptx

Ngày tải lên : 08/08/2014, 20:23

... Annals of General Hospital Psychiatry 2004, 3:14 and the introduction of controlled-current ECT machines The utilisation of muscle relaxants, anaesthetics and resuscitation equipment, and electroencephalographic ... Traditionally, the routine clinical determination of cerebral dominance has been through the assessment of hand, foot and eye dominance It certainly is an easy and inexpensive approach, but it does ... uECT treatment were solely ascertained from handedness assessment, then a small proportion of right-handers and a much larger proportion of left-handers would have treatment administered to the...

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Báo cáo y học: " Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1" docx

Ngày tải lên : 12/08/2014, 01:22

... electrophoresis and comparison of ethidium bromide stain intensity with known DNA standards (Hind III cut lambda phage DNA, New England Biolabs Inc, USA) The nucleic acid extracts were diluted to a standard ... nodes helices and b strands with intermittent unstructured intervening regions The arrangement is characteristic of crystallized hydrolase Domain B is composed of a helix and b strands with intermittent ... http://www.virologyj.com/content/7/1/255 Page of Figure Secondary structure prediction of gp17 protein implications for the welfare of both man and animals Our results may inform new ways of genetic manipulation of phages to...

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$fracture mechanics of concrete concepts, models and determination of material properties$

fracture mechanics of concrete concepts, models and determination of material properties

Ngày tải lên : 24/10/2014, 21:58

... model, crack band model, nonlocal strain-softening models, etc.) are described and random particle simulation o f aggregate microstructure is discussed The principles o f implementation of these models ... (work -of- fracture) method, the Jenq-Shah and Karihaloo-Nallathambi methods, and the size-effect method Experimental determination o f the characteristic length for nonlocal continuum models and ... humidity and temperature, as well as the effect o f cyclic loading Chapter is devoted t o the effect of reinforcing bars and their bond slip on fracture propagation, and t o fracture of fiber-reinforced...

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Analysis and determination of cogging torque and unbalanced magnetic forces in permanent magnet spindle motors for hard disk drives

Ngày tải lên : 15/09/2015, 22:03

... thousands of hours, and tolerate thousands of start and stop cycles without failing Second, it must be able to run smoothly with minimum vibration, due to the tight tolerances of the platters and ... orginal design and the optimal design 169 Table 6.5 Determination of cogging torque period (p = 3) 170 National University of Singapore IX List of Tables Table 6.6 Determination of cogging ... University of Singapore t (2.10) the slot pitch, and 23 Chapter2 Review on Computer Modeling and Analysis of PM Brushless DC Motors half of the peak value of flux reduction in the center of the slot...

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Báo cáo Y học: SF2/ASF protein inhibits camptothecin-induced DNA cleavage by human topoisomerase I potx

Ngày tải lên : 24/03/2014, 03:21

... Binding of htopo I and SF2/ASF to DNA revealed by mobility shift assay (A) Binding of htopo I to DNA Lanes: 1, DNA alone; 2, DNA + 0.14 pmol htopo I (B) Aggregation of DNA by htopo I and SF2/ASF ... smaller amount of covalent DNA htopo I complex formed in the presence of SF2/ASF as a primary reason for the inhibition of camptothecin -induced DNA cleavage Effect of SF2/ASF on DNA relaxation ... °C (A) Inhibition by DNA Lanes: 1, no DNA; 2, 0.15 fmol DNA; 3, 1.5 fmol DNA; 4, 15 fmol DNA (B) Eﬀect of camptothecin at 0.15 fmol DNA Lanes: 1, camptothecin, 2, DNA; 3, DNA + camptothecin with...

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Báo cáo hóa học: " Editorial Novel Techniques for Analysis and Design of Cross-Layer Optimized Wireless Sensor Networks" docx

Ngày tải lên : 22/06/2014, 19:20

... terms of the lifetime of the network The particular application they have in mind is the sensing of a binary event by sensors that are errorprone, and also communicate with each other and a central ... networks” and is a fine example of how an improved modeling of the network can alter our perception of what its optimal operation should be In particular, the authors focus on the construction of backbones ... sacrificing so much of their time in order to make this special issue a success ACKNOWLEDGMENTS We would like to thank the devoted staff of Hindawi for their high level of professionalism, and Philip Regalia,...

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Báo cáo lâm nghiệp: "Extraction and study of enzymes linked to malate metabolism in tree leaves" pdf

Ngày tải lên : 09/08/2014, 04:20

... separation of the two malic enzymes and to detect the presence of isoforms The purification study must continue to evaluate the elution buffer and the purification of samples from different periods of ... leaves of the first flush for oak leaves (May 1988) and on the needles of 1987 for spruce needles The enzymatic capacities increased markedly during the growing season (March) of spruce and during ... DEAE-Trisacryl column (25 x 1.6 cm) at a flow rate of ml/min Fifty fractions of ml were collected and tested for enzyme activities For oak leaves (Fig 2A) and spruce needles (Fig 2B), NADP-malic enzyme...

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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf

Ngày tải lên : 24/03/2014, 04:21

... nicking of pBR-322 DNA by a mixture of ADM, Fe3+, Vc and O2 and by a mixture of (1,10-phenanthroline)-copper(II), ME and O2 converted pBR-322 DNA to small DNA fragments [39] and linear DNA [31], ... interactions of DNA with many small molecules, such as BLM and ADM, are at rapid equilibrium: DNA þ L Ð DNA Á L ð13Þ where L is a small molecule that binds to DNA and DNA L the complex between DNA and ... Thermokinetics of DNA cleavage catalyzed by BLM-A5 (Eur J Biochem 269) 2857 Table Comparison of the molar enthalpy change for the cleavage of calf thymus DNA induced by BLM-A5 and those for the scission of...

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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf

Ngày tải lên : 09/08/2014, 09:20

... that apoptosis induced by agarose is a component of total DNA breakage, and that the inhibition of apoptosis allows for more proper estimation of radiation -induced DNA DSB repair and apoptosis ... technique for the determination of DNA double-strand breaks International journal of radiation biology 1989, 56:437-448 McMillan TJ, Tobi S, Mateos S, Lemon C: The use of DNA double-strand break quantification ... agarose-encapsulated cells The range of DNA quantification, beginning just before the compression zone (CZ) and ending at the bottom of the smears, is shown (B) The amount of released DNA (optical density,...

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Báo cáo y học: "Comparative determination of HIV-1 co-receptor tropism by Enhanced Sensitivity Trofile, gp120 V3-loop RNA and DNA genotyping" ppt

Ngày tải lên : 13/08/2014, 01:20

... (0.110-0.166) (n = 2,946 pairs) and DNA- DNA (n = 2,874) distance was 0.121 (0.095-0.152) The median (IQR) intrapatient RNA-RNA (n = 56), DNA- DNA (n = 52) and paired RNA -DNA (n = 120) distances were ... Study population and samples We processed 178 samples (99 plasma RNA and 79 whole-blood DNA) and successfully amplified 155 samples [78 (78.8%) plasma RNA and 77 (97.5%) whole-blood DNA] from 55 ... the rate of successful sequencing from RNA was 40%, 88.2%, and 96.2% at < = 50 cp/ml, 51-500 cp/ml, and >500 cp/ml respectively The rate of successful sequencing from DNA was 95.1%, 100%, and 100%...

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DETERMINATION OF DNA AND OF OTHER NUCLEIC ACID FRACTIONS

Ngày tải lên : 14/07/2015, 09:44

... Journal of the Institute of Brewing, 1966, 52, 184 17 Trcvelyan, W E., Journal of the Science of Food and Agricui~ lure, 1975, 26, 1673 18 Trevelyan, W E., Journal of the Science of Food and Agricullure, ... such as the high UV absorption of TCA, or the interference of PCA with Kjeldahl digestion DNA + RNA guanine and adenine.—Determinations had a standard deviation of ±0-5-1 jitnol/g By subtracting ... single well-defined standard, a solution of adenine and guanine in 1m perchloric acid, which is stable over a long period of time The separate determination of guanine and adenine has proved...

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DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE

Ngày tải lên : 09/09/2015, 08:16

... the impact of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA damage, we show that the level of DNA damage ... and inset) at and dpi Scale bar 20 lm phosphorylated cH2AX by western blot (Fig 4a) Relative intensities of HA and cH2AX bands were quantified and normalized to b-actin, and the levels of HA and ... SSBs [39, 40] While DNA strand breaks can arise directly via the cleavage of DNA backbone by RONS, strand breaks can also arise via DNA lesions that stall replication forks and generate physical...

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DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE

Ngày tải lên : 30/09/2015, 06:20

... bases, deaminated bases, and DNA- DNA and DNA- protein cross- links (Lonkar and Dedon 2011) In addition, attack of ROS / RNS on DNA backbone can also lead to DNA single strand breaks (SSBs), which ... more harmful DNA strand breaks Taken together, DNA damage (in the form of DNA strand breaks) can be induced directly by ROS / RNS, as well as indirectly during the repair of ROS / RNS -induced base ... modified histones that form DNA repair foci at the sites of DNA strand breaks in the lung tissue of infected mice DNA repair foci are induced in lung epithelial cells and infiltrating immune cells...

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Salts Transport in Alkali Soil Reclamation by Gypsum and Prediction of Na Leaching in Field in China

Ngày tải lên : 05/09/2013, 09:38

... application rates of 0.5 wt% and 1.0 wt% Alkali soil and gypsum were mixed uniformly and were packed in an acrylic ring with an outer diameter of 60 mm, inner diameter of 50 mm, and height of 20 mm at ... conductivity and the transport of salts such as cations (Na+, Ca2+, Mg2+ and K+) and Cl- in a column were investigated by using a tensiometer and a four-electrode sensor, and the amounts of these ... of the soil in the center of the column The inner and outer diameters of the stainless steel rods were mm and 16 mm, respectively, and the ratio (I/V2) of the current of an outer electrode (I)...

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Tài liệu TREES OF THE NORTHERN UNITED STATES THEIR STUDY, DESCRIPTION AND DETERMINATION FOR THE USE OF SCHOOLS AND PRIVATE STUDENTS doc

Ngày tải lên : 13/02/2014, 12:20

... little study of the roots of trees is practicable, some knowledge of their forms, varieties, and parts is important The great office of the roots of all plants is the taking in of food from the ... the pile of plants and paper On account of the size of many leaves and flowerclusters, these pressed specimens of trees should not be shorter than from 12 to 15 inches, and even a length of 18 inches ... objects of nature, provided the work chosen were of a character that would admit of its being studied at all seasons and in all localities, and that the subject were one of general interest, and...

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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

Ngày tải lên : 14/02/2014, 22:20

... Phosphorylation and caspases in DNA fragmentation I Kitazumi and M Tsukahara directly involved in the change in active form of caspases and DNA fragmentation -induced factors [5,11] The induction of DNA fragmentation ... P DNA fragmentation Cleaved PARP Cleavage 150 Cleaved caspase-3 Cleavage Activation Caspase-3 Cleavage Activation Fig Regulation of DNA fragmentation by phosphorylation of the MAPK family and ... Kitazumi and M Tsukahara Involvement of caspases in DNA fragmentation One of the direct substrates of caspases in DNA fragmentation is ICAD Its caspase-mediated cleavage causes the release and activation...

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Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site speciﬁcities and release of matrikines pptx

Ngày tải lên : 16/02/2014, 14:20

... and of 15 possible x-Tyr cleavage sites, respectively x-Phe bonds were found to be hydrolyzed by MMP-7 (2 of 16) and MMP-12 (4 of 16) but not by MMP-9 The cleavage behavior of MMP-7, MMP-9, and ... ‘none’, and the mass error tolerances for precursor and fragment ions were typically set to 0.15 u for qTOF MS ⁄ MS and 50 p.p.m and u, respectively, for MALDI-TOF ⁄ TOF MS data The formation of pyroglutamic ... examples show that it is of utmost importance to understand and characterize elastin-degrading processes, including the cleavage behavior of elastinolytic MMPs and the nature of the peptides released...

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