anatomy of the human heart game
Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... all of the OLP epithelium. The count was performed by two investigators without knowledge of whether the samples were control or experimental. The total number of basal and suprabasal epithelial ... Negative controls consisted of omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification ... OLP would require an increased immunolocalization of hMSH2. Therefore, the reduced expression of hMSH2 protein could make the epithelium of OLP more susceptible to DNA mutation, making it prone...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... spectrophotometric methods.Because the enzymatic activity of PRTFDC1towards hypoxanthine and guanine is only 0.26% and0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism ... activity [10]. The structural characterization of numerous com-plexes of the human HPRT and several bacterial andprotozoan HPRTs have been undertaken [13–17]. The structure of human HPRT can...
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Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
... permonomer of poly(dT), showing that the changes weremainly due to the binding of the first DNA, while the binding of the second DNA had less influence(Fig. 5A). To ensure that the estimation of the ... bymutations on the DNA-binding site of Rad51, suggest-ing that the L2 loop of Rad51 is not far from the DNA-binding site. Consistent with this idea, the fluor-escence of the tryptophan inserted in the ... 1).These results confirm that the residues are close to the DNA-binding site.We then examined if these fluorescence changesoccurred by the binding of the first or second DNA,by titrating these...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... encoded another type of KLK11, isoform 2,despite the presence of exon 1c. The secretory path-ways of KLK11 isoforms appeared to differ, althoughboth isoforms were secreted from cells. Hence, the ... show the transcription initiation sites of the human and a white arrowhead shows the transcription initiation site of the mouse. Dashes show gaps and asterisks show the same nucleotide in the ... mechan-ism of KLK11 gene expression.ResultsThree different primary transcripts are derivedfrom the human KLK11 geneWe determined the nucleotide sequences of 15 clones of the PCR products from the human...
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Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc
... and phylogeny. How-ever, the nature of the connections between thesefactors needs to be understood at the level of the pro-tein domain. The global properties of the HTFNtopology are partially ... property of the bHLH domains. Therefore, this seems to be a topo-logical constrain derived from the evolution of thisfamily.Evolution based on domain reusing might explain the abundance of certain ... TFIIE-a, TFIIE-bF 57 42% Zn finger domains. It contains the 90% of the members of nuclear receptorsuperfamily (they are Zn fingersalso) of the HTFN.14-3-3 zeta, STAT1a, STAT1b, dCREB, ATF-1,FTF,...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... cells. The positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D),respectively, whilst the position of the Oct-1 (98 kDa approximately) and the two ... (2002) Characteri-zation of the 5¢ untranslated region of alpha and betaisoforms of the human thromboxane A2 receptor (TP).Differential promoter utilization by the TP isoforms.Eur J Biochem 269, ... (Prm)3within the human TXA2receptor (TP) gene thatdirects expression of TPb in HEL92.1.7 and HEK293cells [25]. A schematic of the human TP gene highlight-ing the positions of the previously...
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Báo cáo khoa học: Novel repressor of the human FMR1 gene ) identification ¨ of p56 human (GCC)n-binding protein as a Kruppel-like transcription factor ZF5 ppt
... to the protein of interest. In the case of the rpL32 fragmentor the (GCC)9sequence, there are several repeatedZF5-binding sites within a single oligonucleotide.Therefore, the affinity of ... (1994)Identification of a GC element in the promotor of the murine L32 ribosomal protein L32 gene and the 3¢-ter-minal region of the human apolipoprotein A-I gene,capable of binding with murine and human ... Institute of Influenza, Ministry of Health of the Russian Federation. The first pair of primers(5¢-GGCCTTCAAGGCATTAAG-3¢,5¢-AAACAAATGGCCTGTCCG-3¢) spanned a 958 bp 5¢-part of the ZF5coding region; the...
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Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin potx
... epitopes locatedin the MUC5AC C-terminal cysteine-rich part.Further localization of the epitopes of 45M1,2-12 M1 and 166M1In order to further locate the epitopes of the hithertounmapped 45M1, ... detailedmap of the epitopes for the mAbs, where 45M1 recog-nizes an epitope located in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, pre-sumably in the last CysD domain of the ... 5. Mapping of the 45M1, 166M1 and 2-12M1 epitopes on the C-terminal cysteine-rich part of human MUC5AC. The upper part of the figure shows a schematic representation of full-length human MUC5AC...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... subunit of Pph3p required for the binding of Psy2p, we examined the sensitivity of the YBL046w ⁄ Psy4p deletion strainto cisplatin. The comparable increased sensitivities tocisplatin of the homozygous ... was the same as that of the controlstrain transformed with the vector YCplac111 (datanot shown). The YBL046w gene was therefore termedPSY4 (for platinum sensitivity 4). The primary target of ... Superose6 at the same anomalous position of 450 kDa as Dro-sophila His6–R2 (Fig. 3) and human His6–R2 [16].Examination of the phenotype of S. cerevisiaestrains carrying a deletion of the YBL046w...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... per-centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cellcycle after treatment with 20 lM or 100 lM ATP for 24 h. The gat-ing used for the quantification of the cells in the G0 ⁄ ... completelyreversed the inhibition of LXF-289 cell proliferationby ATP. Further indirect support for the involvement of ERK1 ⁄ 2 is derived from the observed translocation of ERK1 ⁄ 2 to the nucleus. The translocation ... detectabledecrease of the p50 form of NF-jB1 in the cytosol after10 min, which is still visible after 60 min (Fig. 5D).Simultaneously with the decrease of p50 in the cytosol,an increase of NF-jB1 p50 in the...
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Báo cáo khoa học: The protein shuffle Sequential interactions among components of the human nucleotide excision repair pathway pdf
... late steps of the process. For example, early in the NER process, RPA assists TFIIH in the opening of the DNA helix around the damage site [36]. Further-more, in the presence of the XPA minimal ... sites.Taken together, these findings suggest that the struc-tural properties of DNA-damaged substrates, whetherintrinsic or the result of protein binding, function in the recruitment of the XPC–hHR23B ... Binding of XPG induces the release of XPC–hHR23B, whereas XPF–ERCC1 triggers exci-sion of the damaged DNA and the release of XPAand TFIIH. Subsequently, the newly formed gap in the DNA is filled by...
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Báo cáo khoa học: Identification of NF1 as a silencer protein of the human adenine nucleotide translocase-2 gene pptx
... in front of the HSVtk promoter and transfected into HeLa cells.CAT activity was normalized for transfection efficiency. The values of the activities are set relative to the activity of the clone ... mL of sample. The column was eluted at0.1 mLÆmin)1. Fractions of 0.5 mL were collected after the void volume of the column.SDS/PAGE and protein identification by MALDI-TOF MSSamples from the ... identifica-tion of NF1 by MALDI-TOF MS is based primarily onpeptide mass matches within the conserved DNA bindingdomain, we cannot distinguish between the variousisoforms. Furthermore, NF1 exist in the...
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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx
... localization in the ER [19]. We found that the TMD of UGT1A pro-teins appended to the C-terminus of the ectodomain of CD4 plasma membrane glycoprotein was able to retain the chimeric protein in the ER ... that disruption of the dilysinemotif KSKTH of UGT1A by mutation of lysine toserine residues or by extending the length of the cytoplasmic tail to relocate the dilysine from the crit-ical positions ... together, these data indicated that the TMD domain of UGT1A was sufficient to retain the ectodomain of CD4 protein in the ER. Because the carbohydrate moieties of proteins that are trans-ported to the...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... of the 15N-1H NOEs were obtainedfrom the difference of two independent experiments. The values of error were adjusted for the proper tensor2 analy-sis, by increasing of the errors from the ... further our knowledge withrespect to the mechanisms of action of these proteins,it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the struc-tures of ... characteristics of the 275–284 segment of hHR23B. Because of the high quality of our15N-HSQC spectra, 54 of the 58 protonated backbonenitrogen atoms were available for relaxation measure-ments. The values...
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Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt
... independent of 15d-PGJ2stimulation.Thereafter, the precise site of action of 15d-PGJ2was identified by further 5¢ deletion analysis dissecting the )192 to )154 region of Prm3. Deletion of the PPARc ... (DR5). These dataprovide direct evidence for the role of PPARc in the regulation of human TP gene expression within the vasculature and point to further critical dif-ferences in the modes of transcriptional ... 15d-PGJ2-suppres-sion of luciferase expression. On the other hand, eitherdeletion of the latter PPARc (b) and RXR IV halfsites, such as within the )154 subfragment, or dis-ruption of the RXR IV half...
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