... of nine random amino acids, followedby a His6-tag in the C-terminus of capsid protein 10.Thereby, the phages display a random nonamer ontheir surface, and by interactions ofthe His6-tag the phages ... dis-tributed along the surfaces ofthe body. They are fre-quently found in the mucosa ofthe airways and intestine, in connective tissue ofthe skin, and aroundblood vessels and nerves. Upon ... number of poten-tial substrates during the screening ofthe full human proteome. This highlights the importance of factorsother than the extended cleavage specificity in deter-mining whether...
... compares the studied network withrandomized versions of it with the same size and degree dis-tribution. The so-called Z-score quantifies the differencebetween the studied network and an ensemble of ... analysis ofthe topological modules ofthe Fig. 3(labelled A–G) shows that they include structural and ⁄or functional features. Table 3 summarizes the main structural and functional features of thesegroups. ... functions, and topologi-cal features retain functionality and phylogeny. How-ever, the nature ofthe connections between thesefactors needs to be understood at the level ofthe pro-tein domain. The...
... telephone with the member of the household who was the sole or co-decisionmaker most knowledgeable about the household’s savings and investments. The standard error for the 2011 sample of households ... 353750525232Age of head of household11 Age is based on the age ofthe sole or co-decisionmaker for household saving and investing.2 For the complete time series of data from 1994 through ... U.S. households3Age of head of household11 Age is based on the age ofthe sole or co-decisionmaker for household saving and investing.2 For the complete time series of data from 1994 through...
... conducted over the telephone with the member ofthe household who was the sole or co-decisionmaker most knowledgeable about the household’s savings and investments. The standard error for the 2012 ... in the appendix. 12 See FigureA10 in the appendix.13 Some ofthe variation in the mutual fund favorability rating across the age groups is affected by differences in the frequency ofthe ... Sentiment, and Use ofthe Internet, 2012.” ICI Research Perspective 18, no. 6 (November). Available at www.ici.org /pdf/ per18-06 .pdf. Ownership of Mutual Funds, Shareholder Sentiment, and Use of the...
... isexpressed as the amount of trehalose-P synthesized in nmolÆmin)1 .The insert shows the Lineweaver–Burk plot ofthe data.Table 2. Comparison ofthe effect of heparin on recombinant and native TPS ... wascompared to the amount of NAD+produced (nmol) in the coupled assay, as an indirect measure ofthe amount of trehalose-P synthesized. However, for most ofthe studiesdescribed here, the anthrone ... constants for TPS The effect of substrate concentration on the activity of the TPS was determined as shown in Figs 6 and 7. Fig. 6 shows the effect of increasing concentrations of either UDP–glucose...
... activity (%)pHFig. 8. Effect ofthe pH on the activity ofthe purified laccase. pH in the range of 2.5–8 were tested with 500 lMABTS (d )and1 7lM of syringaldazine ( j) as the su bstrate.Sd 194 ... was 0.5% of the molecular mass of t he native laccase andand 10% for the recombinant l accase. I n the heterologous production of the P. cinnabarinus lac case in P. past oris [14] or the T. villosa ... are indicated the name ofthe primers u sed for amplification ofthe laccase cDNA and addition of cloning sites, recipient Aspergillus expression vector and restriction sites used i n the final cloning...
... temperature and using high concentrations ofthe weak antagonisttheophylline combined with low ¯ow rates. The ®nal ion-exchange step allowed removal of theophylline, and areduction of volume and detergent ... corresponds to the N-terminus ofthe m ature maltose-binding protein. Binding of the fusion protein to the IMAC gel in buffer containing50 mMimidazole indicates the presence ofthe C-terminalhistidine ... achieved.Ligand binding studies The quanti®cation of interactions with speci®c ligandsallows the structural integrity and homogeneity of a puri®edreceptor p rotein to be judged. The M-A2aTr316-H10...
... rooting ofthe fault into the asthenosphere and the interaction between the fault kinematics andthe deformation ofthe asthenosphere due to plate motion remains open. Modified fromTeyssier and Tikoff ... rifting ofthe lithosphere (Wernicke, 1985). The relative thinning ofthe mantle (Ž) andofthe crust (þ) varies across the rift and may result in the development of a stiff heterogeneity below the ... cold, thick, and consequently stiff lithosphere. On the other hand, riftingmay also modify the thermal structure ofthe lithosphere. Depending on the relative stretching ofthe crust and uppermantle,...
... transcript of apredicted size of 13 kb. Both human MUC4 and mouseMuc4 genes are virtually identical in terms ofthe class of introns, the exon number and size of exons. The sizes of the 24 mouse ... mappingexperiments. The last exon is composed of a codingsequence of 193 bp andof an untranslated region of atleast 131 bp. The size ofthe 24 introns ranges from 79 bp toabout 16 kb. The size ofthe largest ... obtained and studiedbut their analysis showed that they did not contain the central region or the 5¢ end ofthe gene. The cosmid clonecontaining the longest part ofthe new gene was namedCAR1 and...
... expres-sion ofthe constructs under the control ofthe CMV promoter; filledcolumns, HA-tagged constructs under the control ofthe weakerPminpromoter. (B) Immunoblot of B2Rwt, mutant 2 ⁄ 1 andthe ... throughchanges in the overall structure ofthe receptor. Thus,these data demonstrate the connection between the structure of ICLs and that ofthe binding site, implyingreciprocally that changes at the binding ... depend on the cell type andthe receptor expression levels. Although the processes of signaling and regulation of human B2R are, in general, fairly well understood, the knowl-edge ofthe molecular...
... Constitutive expression ofthehuman peroxiredoxin Vgene contributes to protection ofthe genome fromoxidative DNA lesions and to suppression of transcription of noncoding DNAAndrey Kropotov1,2,3, ... bars)shows the results of analysis of 8-oxoG in the control and KD-1 cells. In the KD-1 cells, the concentration of endogenous 8-oxoG was significantly increased,indicating that PRDX5 contributed to the ... cellsdecreases formation of 8-oxoG in mitochondrial DNA[20], but the role of this protein in protecting nuclearDNA from oxidation in human cells andthe mecha-nisms of regulation ofthe PRDX5 gene are...
... [34] between the fruit fly andthe mosquito, not only the basic principles of redox metabolism andthe genomicorganization ofthe TrxR gene, but also the mechanisticpeculiarities of these orthologous ... reaction ofthe cata-lytic cycle, of course, represents the net reaction catalyzed byTrxR.Discussion The isolation and characterization of A. gambiae TrxRcontributes to the understanding ofthe ... support the assumption that the substitution ofthe Trx system for GR, as well as the mechanistic particularities ofthe TrxR, are a commonprinciple in dipteran insects. In the context ofthe malarialsystem,...