analytical approaches towards the structural characterization of microbial wall glycopolymers
Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
Ngày tải lên :
18/02/2014, 06:20
... and the protection level increased towards the C-terminus Furthermore, resonances of these last five residues became almost undetectable in the presence of Mn2+ The solvent-exposed C-terminus of ... implications of the observed VpUcyt structure One of the most studied activities of VpU is the induction of CD4 degradation in the endoplasmatic reticulum of infected CD4+ cells Only the cytoplasmic ... elements bear structural similarity to the tight loop formed by L73–V78 of VpUcyt in the presence of DPC micelles Although the structural motifs adopted by the cytoplasmic domain of VpU appear...
- 16
- 632
- 0
Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf
Ngày tải lên :
19/02/2014, 17:20
... three structures are the complexes with the eNOS peptide, the smMLCK peptide and the CaMKI peptide All three of these are structural rel˚ atives and show rmsds of the Ca atoms of 1.705 A ˚ comparing ... PhK13, which lies towards the N terminus of the C-terminal extension (Fig 2), contains a sequence that Fig An overview of the PhKc domain structure The first 296 residues of the subunit encode ... Met72 Fig (Top) Overlay of two spectra from the titration of Ca2+ ⁄ CaM with PhK5 peptide The red spectrum shows the position of peaks prior to the addition of PhK5 peptide The blue spectrum shows...
- 12
- 590
- 0
Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae pot
Ngày tải lên :
08/03/2014, 02:21
... FEBS 2003 Minor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in b a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2 experiments) ... PEtn attached to the phosphate group at O-4 of Kdo [11,13,16] Structure of the core region of the Hep3-glycoforms In the 1H NMR spectrum of OS-1 (Fig 5A), anomeric resonances of HepI–HepIII were ... were found for the residue probably as a result of differences in the chemical environments of the various glycoforms The first spin-system is attributed to the Hex2 glycoform, while the second spin-system...
- 13
- 433
- 0
Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt
Ngày tải lên :
16/03/2014, 16:20
... NMR characterization of the Nogo-A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation of the domain organization of the human Nogo-A protein (A) The domain organization of ... conformation for the peptide NMR spectroscopy was further utilized to explore the structural properties of Nogo-40 The very narrow resonance dispersion of amide protons ( 0.7 p.p.m) and the lack of side-chain ... molecular mass of Ó FEBS 2004 NMR characterization of the Nogo-A functional domains (Eur J Biochem 271) 3517 Fig NMR spectral assignment of Nogo-40 The NH-aH region of a NOESY spectrum of Nogo-40...
- 11
- 493
- 0
Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx
Ngày tải lên :
16/03/2014, 18:20
... the occurrence of the free CP43 subunit in the fraction A of the sucrose gradient (Fig 1B, lane A) The absence of other peripheral densities in the top-view projection map of the red algal PSII ... and D1, and the extrinsic proteins of the oxygen-evolving complex the 33 kDa, cyt c550 and 12 kDa The presence of the cyt c550 in the PSII fraction was confirmed by heme staining of the gel (Fig ... front edge of the main peak of PSII represents the PSI contaminant Samples of PSII complexes for electron microscopy were collected from the maximum of the main peak of the gel filtration The 77K fluorescence...
- 9
- 426
- 0
Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx
Ngày tải lên :
17/03/2014, 10:20
... were identical with those of authentic reference glycoses, and further confirms their characterization The combined MS data and the isolation of the two aminoglycoses with the respective D-galacto ... is of note that the structure of the LPS O-antigen of F columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... J1,2 8.8 Hz The correlation of H2C to the C2C at 55.4 p.p.m is consistent with the presence of a C2 acetamido substituent, and the lack of a proton at C6, considered in conjunction with the long-range...
- 7
- 405
- 0
Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx
Ngày tải lên :
23/03/2014, 09:21
... with the native state of the protein We then carried out the characterization of the thermal properties of the mutant in comparison with those of PfPNP The results obtained indicate that the substitution ... important for the enzyme stability Finally, the characterization of the thermal properties of the C254S ⁄ C256S mutant allowed us to propose that the CXC motif in the C-terminal region of PfPNP may ... oxidation-reduction to rescue the possible damage of the other two disulfide bonds The presence of a low percentage of the protein with Cys254 and Cys256 in the reduced form further supports this hypothesis It has...
- 14
- 384
- 0
Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx
Ngày tải lên :
23/03/2014, 11:20
... for formation of short helices, transiently, and the chain as such is highly dynamic Structure of the core of the oligomers Because of the large size of the core of the oligomer, the NMR spectra ... assembles in the form of rings around the necks of budding vesicles, and then a conformational change in the dynamin collar aids the scission of the vesicle from the parent membrane The coiled-coil ... signals from the 392 interior of the core and thus not give any information on its structural details Nevertheless, we did derive useful insights into the structural aspects of the core of the oligomer...
- 10
- 370
- 0
Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
Ngày tải lên :
23/03/2014, 13:20
... about the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this ... form the membrane subcomplex, while the remaining four subunits (NdhH–K) form the connecting subcomplex, both of them homologous to the bacterial subcomplexes The theoretical molecular mass of the ... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate...
- 12
- 431
- 0
Báo cáo khoa học: Identification and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus influenzae pptx
Ngày tải lên :
23/03/2014, 21:21
... excitation of H-1 H-resonance of the GlcNAc residue in the TOCSY step and of H-3/H-4 1H-resonance of the GlcNAc residue in the NOESY step (A) The assignments of the 1H-resonances of the Gal II ... Sial-lNnT unit is linked to the Hep I residue of the LPS inner core in the sialylated glycoform The structure of the sialylated glycoforms of the O-deacylated LPS of H influenzae strain RM118 and mutants ... perhaps the levels of sialylated species were over-estimated in the MS studies due to the presence of the sialic acid residue in these glycoforms that would be more readily ionized in the negative...
- 11
- 579
- 0
A STUDY ON THE STRUCTURAL FEATUES OF ENGLISH NEWS STORY.DOC
Ngày tải lên :
02/09/2012, 11:12
... “Structure is an art of the performance on content factors of the writers Structurally, the writers can change the orders of the contents of the news story” In fact, the paragraph of a news story ... newspaper Neither the advent of television nor that of internet could affect the importance of the newspaper The reason for it is the width, breadth and the depth of news provided by the newspaper, ... shows the reader the information of a new online game, the next Internet phenomenon to emerge from computers of college students” The characters of the timeliness, the immediacy, the impact and the...
- 62
- 1.1K
- 5
A STUDY ON THE STRUCTURAL FEATUES OF ENGLISH NEWS STORY
Ngày tải lên :
21/12/2013, 14:47
... “Structure is an art of the performance on content factors of the writers Structurally, the writers can change the orders of the contents of the news story” In fact, the paragraph of a news story ... level The main conveyor of news is newspaper Neither the advent of television nor that of internet could affect the importance of the newspaper The reason for it is the width, breadth and the depth ... write the thesis This part includes the aims, scopes, methods and design of the study Part B is the main part of the study that consists of three chapters below • Chapter I focuses on the theories...
- 62
- 377
- 0
Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
Ngày tải lên :
18/02/2014, 17:20
... homology with other M-superfamily conotoxins Nevertheless, the cDNA structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved ... cleavage of the signal peptide and the propeptide, which happens in the maturation process for every conotoxin [8], the removal of the two additional residues Leu and Val at the C-terminus is rather ... forming a loop (Fig 4), so that the rest of the peptide chain of l-Phe13-conomarphin extends roughly along the axis orientation of the C-terminal helix The structures of several d-amino acid-containing...
- 12
- 616
- 0
Báo cáo khoa học: "TOWARDS THE AUTOMATIC IDENTIFICATION OF ADJECTIVAL SCALES: CLUSTERING ADJECTIVES ACCORDING TO MEANING" ppt
Ngày tải lên :
08/03/2014, 07:20
... that the values of the metrics are related to the number of clusters used, and that the humanjudges were allowed to select the number of clusters they considered most appropriate; consequently, the ... cases of pairs Furthermore, some of the associations between adjectives that the system reports appear to be more stable than others; these associations remain in the same group when we vary the ... incorrectness values These sums use correctness values for the quantities in the first column of Table and incorrectness values for the quantities in the second column of Table Furthermore, the summation...
- 11
- 379
- 0
Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deficient families from Southern Italy docx
Ngày tải lên :
16/03/2014, 01:20
... correlation between the PAH genotype and phenotype should be predicted on the basis of the monogenic nature of the disorder, as was the case in 76% of our patients In the remaining cases, there was a ... Arg408) (Fig 2B) The replacement of a hydrophilic Gln with a rigid Pro residue at the center of the Ca8 helix markedly disturbs the structure of the helix itself Indeed, if not breaking the helix architecture, ... as well as other neurological abnormalities [3] The severity of PAH deciency is variable and partly depends on the nature of the mutations of the PAH gene Recently, a novel subtype of PAH deciency,...
- 12
- 491
- 0
Báo cáo khoa học: "Towards the Unsupervised Acquisition of Discourse Relations" pptx
Ngày tải lên :
16/03/2014, 20:20
... triple consisting of the event type of the utterance, the relation word, and the event type of the preceding utterance – add the candidate triple to the BKB, if it found in the BKB, increase ... automatically parsed corpora While the relation word is usually part of the utterance that represents the source of the relation, determining the appropriate target (antecedent) of the relation may be difficult ... where the factual target of the relation was non-adjacent) are equally distributed, they should be filtered out by the significance tests The goal of this paper is to evaluate the validity of this...
- 5
- 341
- 0
Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf
Ngày tải lên :
17/03/2014, 10:20
... active conformation of the hormone [38,40] The changes engineered in the sequence of the peptide, either through the nature of the side chain of the new amino acids or through their non-native conformational ... minimization (REM) Structural characteristics imposed by the introduction of the novel residues in the sequence of the molecule and the differences between the CRH analogue and the native structure ... structure of a CRH synthetic analogue in TFE/H2O (Eur J Biochem 269) 6013 Fig (Continued) Distribution of electrostatic charges on the surface and along the principal axis of the molecule In the synthetic...
- 11
- 515
- 0
Báo cáo khoa học: "INIGATION OF PROCESSING STRATEGIES FOR THE STRUCTURAL ANALYSIS OF ARGOMF" pdf
Ngày tải lên :
17/03/2014, 19:21
... popping off elements of the stack that are also sons and push the resulting tree onto the stack 2b) Otherwise, push ~ onto the stack In short, search for sons: when one son is found, all of them ... l )The city is awful 2)First, no one cleans the parks 3)So the parks are ugly 4)Then, the roads are ugly, too 5)There's always garbage there But sometimes, )=he"multiple" sons are not brothers of ... u g h the list of prior propositions With these clues, the search is (i) guaranteed to find ~ prior proposition wtlic~ relates to the one with the clue (2) restricted even further due to the semantics...
- 6
- 373
- 0
hydrothermal synthesis and structural characterization of fe2o3sno2 nanoparticles
Ngày tải lên :
19/03/2014, 16:48
... suggests the extent of the solubility of Sn4þ in the a-Fe2O3 lattice At x , 0:7 the rutile phase structure becomes dominant The lattice parameters c and a, of the tetragonal SnO2 cell contract as the ... splitting DEQ of 0.29 mm/s and isomer shift d , ¨ 0:31 mm=s; is characteristic of the Mossbauer spectrum of the hematite structure [15] The continuous line represents the fit of the hypothesized Lorentzian ... lineshape The presence of Sn4þ ions in the system increases the complexity of the computer fit For the fit, we have to consider the preference of tin ions for octahedral positions as well as that the...
- 9
- 394
- 0